Hypoxia and HIF-1 α regulate endoplasmic reticulum (ER) stress in nucleus pulposus cells: Implications of ER stress for extracellular matrix secretion

ER stress is shown to promote nucleus pulposus (NP) cell apoptosis and intervertebral disc degeneration. However, little is known about ER stress regulation by the hypoxic disc microenvironment and its contribution to extracellular matrix homeostasis. NP cells were cultured under hypoxia (1% pO2) to assess ER stress status and gain- and loss-of-function approaches were used to assess the role of HIF-1 α in this pathway. Additionally, the contribution of ER stress induction on the NP cell secretome was assessed by a non-targeted quantitative proteomic analysis by SWATH-mass spectrometry.

Source: American Journal of Pathology - December 8, 2020 Category: Pathology Authors: Emanuel J. Novais, Hyowon Choi, Vedavathi Madhu, Kaori Suyama, Sandra I. Anjo, Bruno Manadas, Irving M. Shapiro, Ant ónio J. Salgado, Makarand V. Risbud Source Type: research