Probiotics exert reciprocal effects on autophagy and interleukin-1 β expression in Salmonella-infected intestinal epithelial cells via autophagy-related 16L1 protein

Beneficial Microbes, Ahead of Print.
Source: Beneficial Microbes - Category: Gastroenterology Authors: Source Type: research

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koh Disease outbreaks caused by the ingestion of contaminated vegetables and fruits pose a significant problem to human health. The sources of contamination of these food products at the preharvest level of agricultural production, most importantly, agricultural soil and irrigation water, serve as potential reservoirs of some clinically significant foodborne pathogenic bacteria. These clinically important bacteria include: Klebsiella spp., Salmonella spp., Citrobacter spp., Shigella spp., Enterobacter spp., Listeria monocytogenes and pathogenic E. coli (and E. coli O157:H7) all of which have the potential to cause dise...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Review Source Type: research
Publication date: January 2020Source: Microbial Pathogenesis, Volume 138Author(s): Chamith Hewawaduge, Amal Senevirathne, John Hwa LeeAbstractIn the present study, the importance of sodium bicarbonate antacid as an agent for an orally delivered attenuated Salmonella strain secreting Brucella antigens Cu–Zn superoxide dismutase (SodC) and outer membrane protein 19 (Omp19) as a live vaccine candidate against Brucella infection was investigated. First, Brucella antigens SodC and Omp19 were cloned into a prokaryotic constitutive expression vector, pJHL65. Then secretion of proteins was verified after transformation into ...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
In this study, sixty significant differentially expressed genes were screened between susceptible and resistant sheep macrophages by transcriptome RNA-seq. Eight significantly enriched GO terms and six canonical pathways were involved by GO and KEGG enrichment analysis. Furthermore, the function of HMOX1, SLPI and SPP1 was verified by siRNA, which the knockdown of HMOX1 and SLPI increased remarkably the clearance of S. typhimurium, but SPP1 had little effect on the clearance of S. Typhimurium within sheep macrophages. Altogether, these results suggest that many genes of macrophages were reprogrammed via S. Typhimurium infe...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
ConclusionsWe found that EFVF is safe with regard to acute toxicity in rats as well as genotoxicity such as mutagenesis or clastogenesis under the present experimental conditions. These results might support the safety of EFVF as a potential therapeutic material for the traditional use or pharmaceutical development.Graphical abstract
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
Publication date: Available online 8 November 2019Source: Materials Today CommunicationsAuthor(s): Maruthupandy Muthuchamy, Thillaichidambaram Muneeswaran, Rajivgandhi Govindan, Quero Franck, Anand Muthusamy, Song Ji-MingAbstractIn the present study, we report CuO and ZnO nanoparticles (NPs) synthesized through biological route using Camellia japonica plant leaf extract and their efficiency detection of cysteine and dihydronicotinamide adenine dinucleotide (NADH) in addition to their antimicrobial properties. Changes in absorption peak intensity in the presence of cysteine or NADH was assessed by UV-Visible spectrophotomet...
Source: Materials Today Communications - Category: Materials Science Source Type: research
The objectives of this study were to evaluate the antimicrobial activity of 10 essential oil compounds and to determine the combined effects with a commercial bacteriophage (SALMONELEX) against Salmonella. Thymol and carvacrol were the most active compounds tested, with a minimum inhibitory concentration (MIC) of 0.25-1 mg/ml and a minimum bactericidal concentration (MBC) of 0.5-1 mg/ml against three Salmonella strains. Multiplicity of infection (MOI), or bacteriophage/pathogen ratio (PFU/CFU), significantly affected bacteriophage efficacy in media and in chicken products. The bacteriophage decreased Salmonella Typhimurium...
Source: Food Control - Category: Food Science Source Type: research
This report outlines the development of a rapid multiplex real-time PCR procedure that facilitates the identification of Salmonella serogroup I and the serovars of the group. Using Salmonella Gaminara serovar (O16:d:1,7) as an example, first the gene(s) responsible for expression of the somatic O antigen, O16, and the nucleotide sequence of the variable-region of genes encoding the flagellar phase-1 (d) and phase-2 (1,7) antigens were identified. Then, a multiplex real-time PCR was designed that incorporated primers and probes specific for the three target genes and confirmed the specificity. The assay had 100% inclusivity...
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research
In this study, we reported a colorimetric biosensor for ultrasensitive detection of Salmonella Typhimurium using a magnetic grid separation column to efficiently separate target bacteria from large volume of sample and platinum loaded zeolitic imidazolate framework-8 (Pt@ZIF-8) nanocatalysts to effectively amplify biological signal. The target Salmonella cells in large volume of sample were first separated and concentrated using the magnetic grid separation column with immune magnetic particle chains, then conjugated with the immune Pt@ZIF-8 nanocatalysts to mimic peroxidase for catalysis of hydrogen peroxide-3,3′,5,...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research
The CDC have recently published information regarding a multi-state Salmonella outbreak. The infections are linked with ground beef consumption.
Source: Health News from Medical News Today - Category: Consumer Health News Tags: Nutrition / Diet Source Type: news
ConclusionThe lack of evidence-based gold standards for the diagnosis of SAD represents a challenge in clinical practice. In our cohort, we confirmed the insufficient correlation between different methods of specific PPV response measurement, and showed that theS. typhi Vi response was not contributive. Caution in the interpretation of results is warranted until more reliable diagnostic methods can be validated.
Source: Journal of Clinical Immunology - Category: Allergy & Immunology Source Type: research
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