Facile fabrication of zwitterionic magnetic composites by one-step distillation-precipitation polymerization for highly specific enrichment of glycopeptides.

Facile fabrication of zwitterionic magnetic composites by one-step distillation-precipitation polymerization for highly specific enrichment of glycopeptides.
Anal Chim Acta. 2019 Apr 11;1053:43-53
Authors: Ji Y, Lv R, Song S, Huang J, Zhang L, Huang G, Li J, Ou J
Abstract
Hydrophilic interaction chromatography (HILIC) utilizing zwitterion-modified material as solid phase has attracted extensive attention for selective enrichment of glycopeptides. However, a tedious synthesis and low specificity for glycopeptides have restricted its application. Herein, a facile and effective approach was developed to synthesize a zwitterionic (ZIC) polymer-coated magnetic composites (denoted as Fe3O4@PMSA) with a zwitterion ((2-(methacryloyloxy)ethyl)-dimethyl-(3-sulfopropyl) ammonium hydroxide, MSA) via one-step distillation-precipitation polymerization (DPP). The well-designed composites presented clearly ZIC-polymer shell and superior hydrophilicity (water contact angle 30.2), and the performance for selective enrichment of glycopeptides were investigated with standard and real samples, respectively. Owning to the abundant of ZIC molecules with multi-charge and polar groups on the surface of resulting polymer coating, the Fe3O4@PMSA demonstrated high selectivity for glycopeptides enrichment with IgG digest (twenty glycopeptides identified, S/N ≥ 20) and a mixture of IgG and BSA at the mass ratio of 1:230 (sixteen glycopeptides identified, S/...

Source: Analytica Chimica Acta - February 5, 2019 Category: Chemistry Authors: Ji Y, Lv R, Song S, Huang J, Zhang L, Huang G, Li J, Ou J Tags: Anal Chim Acta Source Type: research