Influence of Different Cytokinins on the Growth, [6]-Gingerol Production and Antioxidant Activity of in vitro Multiple Shoot Culture of Ginger (Zingiber officinale Roscoe)

The growth, [6]-gingerol production and antioxidant activity of ginger multiple shoots cultured in MS medium containing 1 mg L–1 α-naphthalene acetic acid (NAA) in combination with varying levels of different cytokinins, namely, benzylaminopurine (BAP), kinetin (KIN) and thidiazuron (TDZ) were determined. The [6]-gingerol content ranged from 125-344 μg per culture bottle, with the highest production found in ginger shoot cultures planted in MS medium containing 2 mg L–1 BAP. Growth was inversely correlated to [6]-gingerol production attributed to the negative effects of oxidized phenols on plant development. The antioxidant activity, as determined by FRAP assay, was highly correlated with total phenolics and [6]-gingerol content.
Source: Research Journal of Medicinal Plant - Category: Complementary Medicine Source Type: research