EuroClonality-NGS Recommendations for Evaluation of B-Cell Clonality Analysis by Next-Generation Sequencing

Next-generation sequencing (NGS)-based clonality analysis allows in-depth assessment of the clonal composition of a sample with high sensitivity for detecting small clones. Within the EuroClonality –NGS working group, a protocol for NGS Ig clonality analysis was developed and validated previously. This NGS-based approach was designed to generate small amplicons, making it suitable for samples with suboptimal DNA quality, especially material derived from formalin-fixed, paraffin-embedded tiss ue. Using expert assessment of NGS Ig clonality results as a reference, a structured algorithmic approach to the assessment of NGS-amplicon–based B-cell clonality analysis was developed.

Source: Journal of Molecular Diagnostics - July 17, 2023 Category: Pathology Authors: Michiel van den Brand, Markus M öbs, Franziska Otto, Leonie I. Kroeze, David Gonzalez-de Castro, Kostas Stamatopoulos, Frederic Davi, Clotilde Bravetti, P. Martijn Kolijn, Elisavet Vlachonikola, J. Peter Stewart, Christiane Pott, Michael Hummel, Nikos Da Tags: Regular article Source Type: research