EuroClonality-NGS recommendations for evaluation of B cell clonality analysis by next-generation sequencing – a structured approach with the DEPART algorithm

Next generation sequencing (NGS)-based clonality analysis allows in-depth assessment of the clonal composition of a sample with a high sensitivity for detecting small clones. Within the EuroClonality-NGS working group, a protocol for NGS IG clonality analysis has been previously developed and validated. This NGS-based approach was designed to generate small amplicons, making it suitable for samples with suboptimal DNA quality, especially material derived from formalin-fixed paraffin-embedded tissue.

Source: Journal of Molecular Diagnostics - July 17, 2023 Category: Pathology Authors: Michiel van den Brand, Markus Mobs, Franziska Otto, Leonie I. Kroeze, David Gonzalez-de Castro, Kostas Stamatopoulos, Frederic Davi, Clotilde Bravetti, P. Martijn Kolijn, Elisavet Vlachonika, J Peter Stewart, Christiane Pott, Michael Hummel, Nikos Darzent Tags: Regular Article Source Type: research