Neuroactivity screening of botanical extracts using microelectrode array (MEA) recordings
Food Chem Toxicol. 2024 Jan 6:114438. doi: 10.1016/j.fct.2024.114438. Online ahead of print.ABSTRACTToxicity testing of botanicals is challenging because of their chemical complexity and variability. Since botanicals may affect many different modes of action involved in neuronal function, we used microelectrode array (MEA) recordings of primary rat cortical cultures to screen 16 different botanical extracts for their effects on cell viability and neuronal network function in vitro. Our results demonstrate that extract materials (50 μg/mL) derived from goldenseal, milk thistle, tripterygium, and yohimbe decrease mitochondr...
Source: Food and Chemical Toxicology - January 8, 2024 Category: Food Science Authors: Regina G D M van Kleef Michelle R Embry Constance A Mitchell Remco H S Westerink Source Type: research
Non-targeted chemical analysis of consumer botanical products labeled as blue cohosh (Caulophyllum thalictroides), goldenseal (Hydrastis canadensis), or yohimbe bark (Pausinystalia yohimbe) by NMR and MS
Anal Bioanal Chem. 2023 Nov 1. doi: 10.1007/s00216-023-05004-y. Online ahead of print.ABSTRACTConsumers have unprecedented access to botanical dietary supplements through online retailers, making it difficult to ensure product quality and authenticity. Therefore, methods to survey and compare chemical compositions across botanical products are needed. Nuclear magnetic resonance (NMR) spectroscopy and non-targeted mass spectrometry (MS) were used to chemically analyze commercial products labeled as containing one of three botanicals: blue cohosh, goldenseal, and yohimbe bark. Aqueous and organic phase extracts were prepared...
Source: Analytical and Bioanalytical Chemistry - November 1, 2023 Category: Chemistry Authors: Giovanni O Quiroz-Delfi Cynthia V Rider Stephen S Ferguson Alan K Jarmusch Geoffrey A Mueller Source Type: research
Molecules, Vol. 28, Pages 2025: Yohimbine Alleviates Oxidative Stress and Suppresses Aerobic Cysteine Metabolism Elevated in the Rat Liver of High-Fat Diet-Fed Rats
ajewski
Yohimbine is a small indole alkaloid derived from the bark of the yohimbe tree with documented biological activity, including anti-inflammatory, erectile dysfunction relieving, and fat-burning properties. Hydrogen sulfide (H2S) and sulfane sulfur-containing compounds are regarded as important molecules in redox regulation and are involved in many physiological processes. Recently, their role in the pathophysiology of obesity and obesity-induced liver injury was also reported. The aim of the present study was to verify whether the mechanism of biological activity of yohimbine is related to reactive sulfur specie...
Source: Molecules - February 21, 2023 Category: Chemistry Authors: Ma łgorzata Iciek Magdalena G órny Magdalena Kota ńska Anna Bilska-Wilkosz Marta Kaczor-Kami ńska Jacek Zagajewski Tags: Article Source Type: research
Evaluation of the Herb-Drug Interaction Potential of Commonly Used Botanicals on the US Market with Regard to PXR- and AhR-Mediated Influences on CYP3A4 and CYP1A2
In this study, hydroethanolic extracts of 30 top-selling botanicals (herbs) commonly used as ingredients of herbal dietary supplements in the US were screened for their potential to activate the human pregnane X receptor (hPXR) and human aryl hydrocarbon receptor (hAhR) and to increase the activities of hPXR- and hAhR-regulated drug metabolizing cytochrome P450 enzymes (i.e., CYP3A4 and CYP1A2, respectively). Of the 30 botanicals tested, 21 induced PXR and 29 induced AhR transcriptional activities. Out of the 21 botanicals that induced hPXR transcriptional activity, 14 yielded >50% induction in CYP3A4 activity at concen...
Source: Journal of Dietary Supplements - August 26, 2022 Category: Nutrition Authors: Mona H Haron Olivia Dale Katherine Martin Bharathi Avula Amar G Chittiboyina Ikhlas A Khan Bill J Gurley Shabana I Khan Source Type: research
Molecules, Vol. 25, Pages 3274: A Fast and Reliable UHPLC –MS/MS-Based Method for Screening Selected Pharmacologically Significant Natural Plant Indole Alkaloids
Molecules, Vol. 25, Pages 3274: A Fast and Reliable UHPLC–MS/MS-Based Method for Screening Selected Pharmacologically Significant Natural Plant Indole Alkaloids
Molecules doi: 10.3390/molecules25143274
Authors:
Danuše Tarkowská
Many substances of secondary plant metabolism have often attracted the attention of scientists and the public because they have certain beneficial effects on human health, although the reason for their biosynthesis in the plant remains unclear. This is also the case for alkaloids. More than 200 years have passed since the discovery of the first alkaloid (morphine), and several thousand s...
Source: Molecules - July 17, 2020 Category: Chemistry Authors: Danu še Tarkowská Tags: Article Source Type: research
A fatal case report of acute yohimbine intoxication
ConclusionOnly a very few number of yohimbine fatalities has been reported so far. In the current case, yohimbine acute intoxication appeared to be the most likely cause of death. In fact, the blood concentration highlighted here is the highest ever reported to date. (Source: Forensic Toxicology)
Source: Forensic Toxicology - December 5, 2019 Category: Forensic Medicine Source Type: research
Biomedical analysis of New Psychoactive Substances (NPS) of natural origin
Publication date: Available online 30 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alfredo Fabrizio Lo Faro, Annagiulia Di Trana, Nunzia La Maida, Adriano Tagliabracci, Raffaele Giorgetti, Francesco Paolo BusardòAbstractNew psychoactive substances (NPS) can be divided into two main groups: synthetic molecules and active principles of natural origin. With respect to this latter group, a wide range of alkaloids contained in plants, mainly from Asia and South America, can be included in the class of NPS of natural origin. The majority NPS of natural origin presents stimulant and/or hallucin...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 31, 2019 Category: Drugs & Pharmacology Source Type: research
