Development of a droplet digital PCR for pertussis toxin locus copy number determination in a genetically-modified Bordetella pertussis strain
Biologicals. 2023 May 5;82:101683. doi: 10.1016/j.biologicals.2023.101683. Online ahead of print.ABSTRACTTo improve pertussis toxin (PT) yield in B. pertussis strains for vaccine production a genetically-engineered strain (gdPT 191-134 strain) with a second copy of the genetically detoxified PT (gdPT) locus was developed. The consistency of the production and genetic stability of the strain when used for vaccine production must be established. We developed two simplex ddPCR assays with PCR systems for ptxA, the target gene present in two copies, and pgm, the reference gene present as a single copy. The ddPCR assay had suff...
Source: Biologicals : Journal of the International Association of Biological Standardization - May 7, 2023 Category: Biotechnology Authors: Rapha ël Esson St éphanie Falque Eric Abachin Steve George Nolwenn Nougarede Source Type: research

Development of a droplet digital PCR for pertussis toxin locus copy number determination in a genetically-modified Bordetella pertussis strain
Biologicals. 2023 May 5;82:101683. doi: 10.1016/j.biologicals.2023.101683. Online ahead of print.ABSTRACTTo improve pertussis toxin (PT) yield in B. pertussis strains for vaccine production a genetically-engineered strain (gdPT 191-134 strain) with a second copy of the genetically detoxified PT (gdPT) locus was developed. The consistency of the production and genetic stability of the strain when used for vaccine production must be established. We developed two simplex ddPCR assays with PCR systems for ptxA, the target gene present in two copies, and pgm, the reference gene present as a single copy. The ddPCR assay had suff...
Source: Biologicals : Journal of the International Association of Biological Standardization - May 7, 2023 Category: Biotechnology Authors: Rapha ël Esson St éphanie Falque Eric Abachin Steve George Nolwenn Nougarede Source Type: research

Development of a droplet digital PCR for pertussis toxin locus copy number determination in a genetically-modified Bordetella pertussis strain
Biologicals. 2023 May 5;82:101683. doi: 10.1016/j.biologicals.2023.101683. Online ahead of print.ABSTRACTTo improve pertussis toxin (PT) yield in B. pertussis strains for vaccine production a genetically-engineered strain (gdPT 191-134 strain) with a second copy of the genetically detoxified PT (gdPT) locus was developed. The consistency of the production and genetic stability of the strain when used for vaccine production must be established. We developed two simplex ddPCR assays with PCR systems for ptxA, the target gene present in two copies, and pgm, the reference gene present as a single copy. The ddPCR assay had suff...
Source: Biologicals : Journal of the International Association of Biological Standardization - May 7, 2023 Category: Biotechnology Authors: Rapha ël Esson St éphanie Falque Eric Abachin Steve George Nolwenn Nougarede Source Type: research

Development of a droplet digital PCR for pertussis toxin locus copy number determination in a genetically-modified Bordetella pertussis strain
Biologicals. 2023 May 5;82:101683. doi: 10.1016/j.biologicals.2023.101683. Online ahead of print.ABSTRACTTo improve pertussis toxin (PT) yield in B. pertussis strains for vaccine production a genetically-engineered strain (gdPT 191-134 strain) with a second copy of the genetically detoxified PT (gdPT) locus was developed. The consistency of the production and genetic stability of the strain when used for vaccine production must be established. We developed two simplex ddPCR assays with PCR systems for ptxA, the target gene present in two copies, and pgm, the reference gene present as a single copy. The ddPCR assay had suff...
Source: Biologicals : Journal of the International Association of Biological Standardization - May 7, 2023 Category: Biotechnology Authors: Rapha ël Esson St éphanie Falque Eric Abachin Steve George Nolwenn Nougarede Source Type: research

Development of a droplet digital PCR for pertussis toxin locus copy number determination in a genetically-modified Bordetella pertussis strain
Biologicals. 2023 May 5;82:101683. doi: 10.1016/j.biologicals.2023.101683. Online ahead of print.ABSTRACTTo improve pertussis toxin (PT) yield in B. pertussis strains for vaccine production a genetically-engineered strain (gdPT 191-134 strain) with a second copy of the genetically detoxified PT (gdPT) locus was developed. The consistency of the production and genetic stability of the strain when used for vaccine production must be established. We developed two simplex ddPCR assays with PCR systems for ptxA, the target gene present in two copies, and pgm, the reference gene present as a single copy. The ddPCR assay had suff...
Source: Biologicals : Journal of the International Association of Biological Standardization - May 7, 2023 Category: Biotechnology Authors: Rapha ël Esson St éphanie Falque Eric Abachin Steve George Nolwenn Nougarede Source Type: research

Molecules, Vol. 28, Pages 3937: Qualitative and Quantitative Analysis of the Major Bioactive Components of Juniperus chinensis L. Using LC-QTOF-MS and LC-MSMS and Investigation of Antibacterial Activity against Pathogenic Bacteria
gseon Kim Plants in the genus Juniperus have been reported to produce a variety of chemical components, such as coumarins, flavonoids, lignans, sterols, and terpenoids. Here, ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) and ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) were applied to qualitatively and quantitatively analyze the major bioactive components in an ethanolic crude extract from the leaves of Juniperus chinensis L., which grows naturally in Korea. In addition, the antibacterial activity of the crude e...
Source: Molecules - May 7, 2023 Category: Chemistry Authors: Da Jung Lim Jeong-Sup Song Byoung-Hee Lee Youn Kyoung Son Yangseon Kim Tags: Communication Source Type: research

The whole-cell proteome shows the characteristics of macrolides-resistant Bordetella pertussis in China linked to the biofilm formation
Arch Microbiol. 2023 May 6;205(6):219. doi: 10.1007/s00203-023-03566-0.ABSTRACTThe macrolides-resistant Bordetella pertussis (MR-Bp) isolates in China evolved from the ptxP1/fhaB3 allele and rapidly became predominant, suggestive of an adaptive transmission ability. This was different from the global prevalent ptxP3 strains, in which MR-Bp was rarely reported. The study aimed to determine the underlying mechanism responsible for fitness and resistance in these two strains. We identify proteomic differences between ptxP1/fhaB3 and ptxP3/fhaB1 strains using tandem mass tag (TMT)-based proteomics. We then performed in-depth b...
Source: Archives of Microbiology - May 6, 2023 Category: Microbiology Authors: Zhe Lv Sha Yin Kaichong Jiang Wei Wang Yang Luan Shuang Wu Jianfei Shi Zhe Li Xiao Ma Zengguo Wang Hong Yan Source Type: research

The whole-cell proteome shows the characteristics of macrolides-resistant Bordetella pertussis in China linked to the biofilm formation
Arch Microbiol. 2023 May 6;205(6):219. doi: 10.1007/s00203-023-03566-0.ABSTRACTThe macrolides-resistant Bordetella pertussis (MR-Bp) isolates in China evolved from the ptxP1/fhaB3 allele and rapidly became predominant, suggestive of an adaptive transmission ability. This was different from the global prevalent ptxP3 strains, in which MR-Bp was rarely reported. The study aimed to determine the underlying mechanism responsible for fitness and resistance in these two strains. We identify proteomic differences between ptxP1/fhaB3 and ptxP3/fhaB1 strains using tandem mass tag (TMT)-based proteomics. We then performed in-depth b...
Source: Archives of Microbiology - May 6, 2023 Category: Microbiology Authors: Zhe Lv Sha Yin Kaichong Jiang Wei Wang Yang Luan Shuang Wu Jianfei Shi Zhe Li Xiao Ma Zengguo Wang Hong Yan Source Type: research

Tetanus, diphtheria, and acellular pertussis (Tdap) vaccine coverage in adults with chronic respiratory conditions
Pertussis is a highly contagious, vaccine-preventable, respiratory disease caused by the bacterium Bordetella pertussis. Despite high infant vaccination rates and the extension of vaccine recommendations to adolescents and adults, the control of pertussis has proven challenging. Pertussis has remained endemic world-wide and upward trends in incidence, with increased intensity of outbreaks, have been documented in multiple countries.1-5 Although most cases of pertussis occur among infants too young to be vaccinated, the global change in the epidemiology of pertussis has been accompanied by a gradual shift in the age-specifi...
Source: Annals of Allergy, Asthma and Immunology - April 18, 2023 Category: Allergy & Immunology Authors: Sarah Naeger, Denis Macina, Vitali Pool Tags: Original Article Source Type: research

Bordetella pertussis
Trends Microbiol. 2023 Apr 4:S0966-842X(23)00092-6. doi: 10.1016/j.tim.2023.03.012. Online ahead of print.NO ABSTRACTPMID:37024321 | DOI:10.1016/j.tim.2023.03.012 (Source: Trends in Microbiology)
Source: Trends in Microbiology - April 6, 2023 Category: Microbiology Authors: Yasmine Fathy Mohamed Kiruthika Manivannan Rachel C Fernandez Source Type: research

Bordetella pertussis
Trends Microbiol. 2023 Apr 4:S0966-842X(23)00092-6. doi: 10.1016/j.tim.2023.03.012. Online ahead of print.NO ABSTRACTPMID:37024321 | DOI:10.1016/j.tim.2023.03.012 (Source: Trends in Microbiology)
Source: Trends in Microbiology - April 6, 2023 Category: Microbiology Authors: Yasmine Fathy Mohamed Kiruthika Manivannan Rachel C Fernandez Source Type: research

Bordetella pertussis
Trends Microbiol. 2023 Apr 4:S0966-842X(23)00092-6. doi: 10.1016/j.tim.2023.03.012. Online ahead of print.NO ABSTRACTPMID:37024321 | DOI:10.1016/j.tim.2023.03.012 (Source: Trends in Microbiology)
Source: Trends in Microbiology - April 6, 2023 Category: Microbiology Authors: Yasmine Fathy Mohamed Kiruthika Manivannan Rachel C Fernandez Source Type: research

Multiple rib and vertebral fractures associated with Bordetella pertussis infection: a case report
We report the first case of mu... (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - April 6, 2023 Category: Infectious Diseases Authors: Jingqiao Wang, Junxiang Gao, Hongwei Fan, Haonan Guo, Zundong Yin, Mei Dong and Xiaoming Huang Tags: Case report Source Type: research

Bordetella spp. utilize the type 3 secretion system to manipulate the VIP/VPAC2 signaling and promote colonization and persistence of the three classical Bordetella in the lower respiratory tract
ConclusionTaken together, our findings uncover a novel mechanism of bacteria-host crosstalk that could provide a target for the future treatment for whooping cough as well as other infectious diseases caused primarily by persistent mucosal infections. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - March 29, 2023 Category: Microbiology Source Type: research