Discovery of a potent and selective human AC2 inhibitor based on 7-deazapurine analogues of adefovir
Bioorg Med Chem. 2023 Oct 26;95:117508. doi: 10.1016/j.bmc.2023.117508. Online ahead of print.ABSTRACTAdefovir based acyclic nucleoside phosphonates were previously shown to modulate bacterial and, to a certain extent, human adenylate cyclases (mACs). In this work, a series of 24 novel 7-substituted 7-deazaadefovir analogues were synthesized in the form of prodrugs. Twelve analogues were single-digit micromolar inhibitors of Bordetella pertussis adenylate cyclase toxin with no cytotoxicity to J774A.1 macrophages. In HEK293 cell-based assays, compound 14 was identified as a potent (IC50 = 4.45 μM), non-toxic, and selective...
Source: Bioorganic and Medicinal Chemistry - November 6, 2023 Category: Chemistry Authors: Pavel Kraina Michal Česnek Eva Tlou šťová Helena Mertl íková-Kaiserová Camryn J Fulton Emily K Davidson Brenton P Smith Val J Watts Zlatko Janeba Source Type: research
Antibody and B-cell immune responses against Bordetella pertussis following infection and immunization
J Mol Biol. 2023 Nov 3:168344. doi: 10.1016/j.jmb.2023.168344. Online ahead of print.ABSTRACTNeither immunization nor recovery from natural infection provides life-long protection against Bordetella pertussis. Replacement of a whole-cell pertussis (wP) vaccine with an acellular pertussis (aP) vaccine, mutations in B. pertussis strains, and better diagnostic techniques, contribute to resurgence of number of cases especially in young infants. Development of new immunization strategies relies on a comprehensive understanding of immune system responses to infection and immunization and how triggering these immune components wo...
Source: Mol Biol Cell - November 5, 2023 Category: Molecular Biology Authors: Bahaa Abu-Raya Mirjam J Esser Eve Nakabembe Jes ús Reiné Kyle Amaral Annieck M Diks Esther Imede Sing Sing Way Ali M Harandi Andrew Gorringe Kirsty Le Doare Scott A Halperin Magdalena A Berkowska Manish Sadarangani Source Type: research
Cost of illness due to pertussis in adults ≥50 years of age in the United Kingdom
CONCLUSION: Total cost of pertussis in the UK among adults ≥50 years of age is substantial and highest for adults 55-59 years of age. Indirect costs were the main contributors to the economic burden.PMID:37839946 | DOI:10.1016/j.vaccine.2023.10.010 (Source: Vaccine)
Source: Vaccine - October 15, 2023 Category: Allergy & Immunology Authors: Versteeg Jan-Willem Jamet Nicolas Redekop Ken Source Type: research
DNA-Dependent Interferon Induction and Lung Inflammation in Bordetella pertussis Infection
Journal of Interferon&Cytokine Research,Volume 43, Issue 10, Page 478-486, October 2023. (Source: Journal of Interferon)
Source: Journal of Interferon - October 9, 2023 Category: Research Authors: Jeremy Ardanuy Karen M. Scanlon Ciaran Skerry Nicholas H. Carbonetti Source Type: research
Combined regulation of pro-inflammatory cytokines production by STAT3 and STAT5 in a model of B. pertussis infection of alveolar macrophages
Bordetella pertussis is a highly contagious respiratory pathogen responsible for whooping-cough or pertussis. Despite high vaccination coverage worldwide, this gram-negative bacterium continues to spread among the population. B. pertussis is transmitted by aerosol droplets from an infected individual to a new host and will colonize its upper respiratory tract. Alveolar macrophages (AMs) are effector cells of the innate immune system that phagocytose B. pertussis and secrete both pro-inflammatory and antimicrobial mediators in the lungs. However, understanding their role in B. pertussis pathogenesis at the molecular level i...
Source: Frontiers in Immunology - September 28, 2023 Category: Allergy & Immunology Source Type: research
Influence of the COVID-19 pandemic measures on incidence and representation of other infectious diseases in Germany: a lesson to be learnt
ConclusionOnline available data provided valuable sources for research purposes in infodemiology or infoveillance. (Source: Journal of Public Health)
Source: Journal of Public Health - September 27, 2023 Category: Health Management Source Type: research
GSE241920 Quantitative PCR (qRT-PCR) analysis of micro RNA expression in mouse cells
Contributors : Kinarm Ko ; Yukyeong LeeSeries Type : Expression profiling by RT-PCROrganism : Bordetella pertussis ; Mus musculusTo comprehend the underlying mechanisms of NSC proliferation and differentiation, microRNA expression was investigated. Total RNA was extracted from NSCs, ESCs, and MEFs, and microRNA expression was examined using RT-PCR. This was accomplished utilizing the System Biosciences' Mouse miRNome Sanger miRVase microRNA Profiler Set. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - September 4, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by RT-PCR Bordetella pertussis Mus musculus Source Type: research
DNA-Dependent Interferon Induction and Lung Inflammation in Bordetella pertussis Infection
Journal of Interferon&Cytokine Research, Ahead of Print. (Source: Journal of Interferon and Cytokine Research)
Source: Journal of Interferon and Cytokine Research - August 31, 2023 Category: Molecular Biology Authors: Jeremy Ardanuy Karen M. Scanlon Ciaran Skerry Nicholas H. Carbonetti Source Type: research
Severe problem of macrolides resistance to common pathogens in China
With the widespread use of macrolide antibiotics in China, common pathogens causing children’s infections, such as Streptococcus pneumoniae, Streptococcus (including Group A streptococcus, Group B streptococcus), Staphylococcus aureus, Bordetella pertussis, and Mycoplasma pneumoniae, have shown varying degrees of drug resistance. In order to provide such problem and related evidence for rational use of antibiotics in clinic, we reviewed the drug resistance of common bacteria to macrolides in children recent 20 years. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - August 10, 2023 Category: Microbiology Source Type: research
Integrating proteomic data with metabolic modeling provides insight into key pathways of Bordetella pertussis biofilms
This study compared the proteomic expression of biofilm and planktonic B. pertussis cells to identify key changes between the conditions. Major differences were identified in virulence factors including an upregulation of toxins (adenylate cyclase toxin and dermonecrotic toxin) and downregulation of pertactin and type III secretion system proteins in biofilm cells. To further dissect metabolic pathways that are altered during the biofilm lifestyle, the proteomic data was then incorporated into a genome scale metabolic model using the Integrative Metabolic Analysis Tool (iMAT). The generated models predicted that planktonic...
Source: Frontiers in Microbiology - August 3, 2023 Category: Microbiology Source Type: research
T cell reactivity to Bordetella pertussis is highly diverse regardless of childhood vaccination
Cell Host Microbe. 2023 Jul 16:S1931-3128(23)00266-4. doi: 10.1016/j.chom.2023.06.015. Online ahead of print.ABSTRACTThe incidence of whooping cough due to Bordetella pertussis (BP) infections has increased recently. It is believed that the shift from whole-cell pertussis (wP) vaccines to acellular pertussis (aP) vaccines may be contributing to this rise. While T cells are key in controlling and preventing disease, nearly all knowledge relates to antigens in aP vaccines. A whole-genome mapping of human BP-specific CD4+ T cell responses was performed in healthy vaccinated adults and revealed unexpected broad reactivity to h...
Source: Cell Host and Microbe - July 25, 2023 Category: Microbiology Authors: Ricardo da Silva Antunes Emily Garrigan Lorenzo G Quiambao Sandeep Kumar Dhanda Daniel Marrama Luise Westernberg Eric Wang Adam Abawi Aaron Sutherland Sandra K Armstrong Timothy J Brickman John Sidney April Frazier Tod J Merkel Bjoern Peters Alessandro Se Source Type: research
T cell reactivity to Bordetella pertussis is highly diverse regardless of childhood vaccination
Cell Host Microbe. 2023 Jul 16:S1931-3128(23)00266-4. doi: 10.1016/j.chom.2023.06.015. Online ahead of print.ABSTRACTThe incidence of whooping cough due to Bordetella pertussis (BP) infections has increased recently. It is believed that the shift from whole-cell pertussis (wP) vaccines to acellular pertussis (aP) vaccines may be contributing to this rise. While T cells are key in controlling and preventing disease, nearly all knowledge relates to antigens in aP vaccines. A whole-genome mapping of human BP-specific CD4+ T cell responses was performed in healthy vaccinated adults and revealed unexpected broad reactivity to h...
Source: Cell Host and Microbe - July 25, 2023 Category: Microbiology Authors: Ricardo da Silva Antunes Emily Garrigan Lorenzo G Quiambao Sandeep Kumar Dhanda Daniel Marrama Luise Westernberg Eric Wang Adam Abawi Aaron Sutherland Sandra K Armstrong Timothy J Brickman John Sidney April Frazier Tod J Merkel Bjoern Peters Alessandro Se Source Type: research
T cell reactivity to Bordetella pertussis is highly diverse regardless of childhood vaccination
Cell Host Microbe. 2023 Jul 16:S1931-3128(23)00266-4. doi: 10.1016/j.chom.2023.06.015. Online ahead of print.ABSTRACTThe incidence of whooping cough due to Bordetella pertussis (BP) infections has increased recently. It is believed that the shift from whole-cell pertussis (wP) vaccines to acellular pertussis (aP) vaccines may be contributing to this rise. While T cells are key in controlling and preventing disease, nearly all knowledge relates to antigens in aP vaccines. A whole-genome mapping of human BP-specific CD4+ T cell responses was performed in healthy vaccinated adults and revealed unexpected broad reactivity to h...
Source: Cell Host and Microbe - July 25, 2023 Category: Microbiology Authors: Ricardo da Silva Antunes Emily Garrigan Lorenzo G Quiambao Sandeep Kumar Dhanda Daniel Marrama Luise Westernberg Eric Wang Adam Abawi Aaron Sutherland Sandra K Armstrong Timothy J Brickman John Sidney April Frazier Tod J Merkel Bjoern Peters Alessandro Se Source Type: research
Ultrastructural analysis of the intracellular surfactant in lungs of healthy and ovalbumin sensitized and challenged < em > Brown Norway < /em > rats
Conclusion: The obtained results suggest that asthma-induced surfactant dysfunction is not related to disturbances in the intracellular surfactant´s ultrastructural correlates.PMID:37480230 | DOI:10.1080/01902148.2023.2238232 (Source: Cell Research)
Source: Cell Research - July 22, 2023 Category: Cytology Authors: Andreas Schmiedl Stefanie Frank Thomas Tschernig Jens M Hohlfeld Source Type: research
Ultrastructural analysis of the intracellular surfactant in lungs of healthy and ovalbumin sensitized and challenged < em > Brown Norway < /em > rats
Conclusion: The obtained results suggest that asthma-induced surfactant dysfunction is not related to disturbances in the intracellular surfactant´s ultrastructural correlates.PMID:37480230 | DOI:10.1080/01902148.2023.2238232 (Source: Experimental Lung Research)
Source: Experimental Lung Research - July 22, 2023 Category: Respiratory Medicine Authors: Andreas Schmiedl Stefanie Frank Thomas Tschernig Jens M Hohlfeld Source Type: research
