Could Bordetella pertussis vaccine protect against coronavirus COVID-19?
Publication date: Available online 16 July 2020Source: Journal of Global Antimicrobial ResistanceAuthor(s): Usama M. Alkholy, Mohamed E Salama, Hagar Mahmoud, Ahmed Taher, Khaled M. Elsayes (Source: Journal of Global Antimicrobial Resistance)
Source: Journal of Global Antimicrobial Resistance - July 18, 2020 Category: Infectious Diseases Source Type: research

Comparison of whole-cell versus acellular pertussis vaccine effectiveness in school clusters of pertussis, France, 2013.
CONCLUSIONS: The limited duration of protection conferred by the acellular vaccine reinforces the 2013 introduction of the pertussis booster at six years old. PMID: 32659333 [PubMed - as supplied by publisher] (Source: Medecine et Maladies Infectieuses)
Source: Medecine et Maladies Infectieuses - July 15, 2020 Category: Infectious Diseases Tags: Med Mal Infect Source Type: research

IJERPH, Vol. 17, Pages 4984: Countering Vaccine Hesitancy among Pregnant Women in England: The Case of Boostrix-IPV
This study explored the effects of message framing on vaccine hesitancy for the antenatal whooping cough vaccine. The study also assessed whether the Theory of Planned Behaviour (TPB) constructs had any explanatory utility for vaccine intentions and behaviours in pregnant women. A between-subjects, cross-sectional design was employed. Participants (n = 282) were women who were pregnant (mean = 28 weeks, SD = 7.0), living in England and between 18 and 44 years of age. A self-report web-based survey was used to collect data. Participants were randomly assigned to read either (i) disease risk, (ii) myth busting, or (iii) cont...
Source: International Journal of Environmental Research and Public Health - July 10, 2020 Category: Environmental Health Authors: Mairead Ryan Laura A V Marlow Alice Forster Tags: Article Source Type: research

Acyltransferase-mediated selection of the length of the fatty acyl chain and of the acylation site governs activation of bacterial RTX toxins [Membrane Biology]
In a wide range of organisms, from bacteria to humans, numerous proteins have to be posttranslationally acylated to become biologically active. Bacterial repeats in toxin (RTX) cytolysins form a prominent group of proteins that are synthesized as inactive protoxins and undergo posttranslational acylation on ε-amino groups of two internal conserved lysine residues by co-expressed toxin-activating acyltransferases. Here, we investigated how the chemical nature, position, and number of bound acyl chains govern the activities of Bordetella pertussis adenylate cyclase toxin (CyaA), Escherichia coli α-hemolysin (Hl...
Source: Journal of Biological Chemistry - July 10, 2020 Category: Chemistry Authors: Adriana Osickova, Humaira Khaliq, Jiri Masin, David Jurnecka, Anna Sukova, Radovan Fiser, Jana Holubova, Ondrej Stanek, Peter Sebo, Radim Osicka Tags: Editors ' Picks Source Type: research

Seasonal Bordetella pertussis pattern in the period from 2008 to 2018 in Germany
After the introduction of a vaccine against B. pertussis the seasonal pattern with the highest number of infections in the spring to summer months changed. Recent studies from around the world suggest that B. per... (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - July 3, 2020 Category: Infectious Diseases Authors: Daniel Allermann Hitz, Friedemann Tewald and Maren Eggers Tags: Research article Source Type: research

Toward an Alum Free Mono-Component Monovalent Pertussis Vaccine: A Cytokine Response Assay.
CONCLUSION: Our alum free mono-component monovalent recombinant fusion protein (LLO-PTS1) could bear the capacity to stimulate the release of IFN-γ similar to wP and aP vaccines in the mouse model. Besides, it showed better results in stimulating the release of IL-17 and IL-4 response. This study can be regarded as a platform for further probes in booster pertussis vaccine development. PMID: 32602465 [PubMed - in process] (Source: Iranian Journal of Immunology)
Source: Iranian Journal of Immunology - July 2, 2020 Category: Allergy & Immunology Tags: Iran J Immunol Source Type: research

Neurologic alterations in an HIV adult patient with pertussis: a case report
Pertussis is a highly contagious disease of public health interest caused by the bacterium Bordetella pertussis. Although its incidence has decreased substantially after the introduction of a vaccination, the bur... (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - July 2, 2020 Category: Infectious Diseases Authors: Mar ía Camila Arango-Granados and Iván Mauricio Trompa Tags: Case report Source Type: research

Pertussis-like syndrome often not associated with Bordetella pertussis: 5-year study in a large children ’s hospital
. (Source: Infectious Diseases)
Source: Infectious Diseases - June 26, 2020 Category: Infectious Diseases Authors: Qin Xiong Shiying Hao Lei Shen Jian Liu Tingting Chen Guoqin Zhang Yu-juan Huang Source Type: research

Immuno-proteomics analysis between OMV of vaccine and dominant wild type strains of Bordetella pertussis in Iran.
Conclusion: OMV Bp could be considered as an alternative vaccine against pertussis, containing the bacterium's protein antigens that can confer equal efficacy compared to a whole bacterial cell vaccine with advantages such as less side effects and lower costs than acellular pertussis vaccines. PMID: 32494341 [PubMed] (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - June 6, 2020 Category: Microbiology Tags: Iran J Microbiol Source Type: research

Development of a targeted nanoLC-MS/MS method for quantitation of residual toxins from bordetella pertussis
Publication date: Available online 28 May 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lisa R. Szymkowicz, Derek J. Wilson, D. Andrew James (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - May 30, 2020 Category: Drugs & Pharmacology Source Type: research

Effectiveness of rapid multiplex polymerase chain reaction for early diagnosis and treatment of pertussis.
CONCLUSION: Testing patients with respiratory symptoms using mPCR can improve early diagnosis of pertussis, ensure proper treatment, and may help with outbreak control. PMID: 32513618 [PubMed - as supplied by publisher] (Source: Journal of Microbiology, Immunology, and Infection)
Source: Journal of Microbiology, Immunology, and Infection - May 28, 2020 Category: Microbiology Authors: Oh SC, Park SM, Hur J, Choi EY, Jin HJ, Kim YK, Lee JH, Ahn JY, Lee JM Tags: J Microbiol Immunol Infect Source Type: research

Pathogen analysis of pertussis-like syndrome in children
The aim of the study was to identify the pathogens, in addition to bordetella pertussis (B. pertussis), which cause pertussis-like syndrome in children and to compare clinical presentation between those with B. p... (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - May 19, 2020 Category: Infectious Diseases Authors: Wenjing Gu, Kun Wang, Xinxing Zhang, Chuangli Hao, Yanhong Lu, Min Wu, Sainan Chen, Yanyu He, Jun Xu, Xuejun Shao and Yuqing Wang Tags: Research article Source Type: research

Comparative genomics of whole-cell pertussis vaccine strains from India
Despite high vaccination coverage using acellular (ACV) and whole-cell pertussis (WCV) vaccines, the resurgence of pertussis is observed globally. Genetic divergence in circulating strains of Bordetella pertussis (Source: BMC Genomics)
Source: BMC Genomics - May 7, 2020 Category: Genetics & Stem Cells Authors: Shweta Alai, Vikas C. Ghattargi, Manish Gautam, Krunal Patel, Shrikant P. Pawar, Dhiraj P. Dhotre, Umesh Shaligram and Sunil Gairola Tags: Research article Source Type: research

GSE137180 RNAseq analyses of BvgA in Bordetella pertussis
We examined the global transcription of B. pertussis in different strains and culture conditions (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - May 1, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Bordetella pertussis Source Type: research

GSE137027 ChIPseq analyses of BvgA in Bordetella pertussis
We examined the BvgA fixation on the genome of B. pertussis in different strains and culture conditions (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - May 1, 2020 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Bordetella pertussis Source Type: research

The first report of isolation of macrolide-resistant Bordetella pertussis in Japan.
PMID: 32350216 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - April 30, 2020 Category: Infectious Diseases Authors: Yamaguchi T, Kawasaki Y, Katsukawa C, Kawahara R, Kawatsu K Tags: Jpn J Infect Dis Source Type: research

Development and Validation of a Bordetella pertussis Whole-Genome Screening Strategy.
Authors: da Silva Antunes R, Quiambao LG, Sutherland A, Soldevila F, Dhanda SK, Armstrong SK, Brickman TJ, Merkel T, Peters B, Sette A Abstract The immune response elicited by the protective whole-cell pertussis (wP) versus the less-protective acellular pertussis (aP) vaccine has been well characterized; however, important clinical problems remain unsolved, as the inability of the currently administered aP vaccine is resulting in the reemergence of clinical disease (i.e., whooping cough). Strong evidence has shown that original, childhood aP and wP priming vaccines provide a long-lasting imprint on the CD4+ T cells...
Source: Journal of Immunology Research - April 25, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain.
Conclusion: The presented technique is a simple and effective way to obtain OMVs from Bordetella pertussis. So it can be utilized as an appropriate procedure in the development of an OMV-based vaccine against pertussis. PMID: 32322378 [PubMed] (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 24, 2020 Category: Microbiology Tags: Iran J Microbiol Source Type: research

Genome diversity and evolutionary characteristics of clinical isolates of Bordetella pertussis circulating in Iran.
Conclusion: No changes in allelic profile of predominant clone in recent years was observed but antigenic divergence between recently circulating isolates and the vaccine strain has been progressed and significantly was higher than previous studies. The comparative genomic analysis of the ptxP3 and ptxP1 isolates indicate that changes in ptxP3 genome structure including 32 unique SNPs and three unique indels may have contributed to the expansion of the ptxP3 clone. We compared ptxP3 and ptxP1 isolates in pathogenicity-associated genes and found five of them were specific for the ptxP3 isolates. The polymorphisms in pathoge...
Source: Iranian Journal of Microbiology - April 24, 2020 Category: Microbiology Tags: Iran J Microbiol Source Type: research

Development and Standardization of a High-Throughput Bordetella pertussis Growth-Inhibition Assay
Bordetella pertussis, the main causative agent of whooping cough, is a reemerging pathogen, and recent vaccine-resistant strain outbreaks and emergence of macrolides-resistant strains in China raised new concerns for control of the disease. New vaccines and potentially new antibiotics are thus needed. B. pertussis is tedious to culture and requires several days of growth to count isolated colonies on agar-based media, making large-scale screening of new anti-B. pertussis compounds or functional evaluation of large sample sizes of immune sera difficult. Here, we developed a scalable, rapid, high-throughput luminescence-base...
Source: Frontiers in Microbiology - April 23, 2020 Category: Microbiology Source Type: research

Multicenter Evaluation of the QIAstat-Dx Respiratory Panel for Detection of Viruses and Bacteria in Nasopharyngeal Swab Specimens [Virology]
The QIAstat-Dx Respiratory Panel (QIAstat-Dx RP) is a multiplex in vitro diagnostic test for the qualitative detection of 20 pathogens directly from nasopharyngeal swab (NPS) specimens. The assay is performed using a simple sample-to-answer platform with results available in approximately 69 min. The pathogens identified are adenovirus, coronavirus 229E, coronavirus HKU1, coronavirus NL63, coronavirus OC43, human metapneumovirus A and B, influenza A, influenza A H1, influenza A H3, influenza A H1N1/2009, influenza B, parainfluenza virus 1, parainfluenza virus 2, parainfluenza virus 3, parainfluenza virus 4, rhinovirus/ente...
Source: Journal of Clinical Microbiology - April 23, 2020 Category: Microbiology Authors: Leber, A. L., Lisby, J. G., Hansen, G., Relich, R. F., Schneider, U. V., Granato, P., Young, S., Pareja, J., Hannet, I. Tags: Virology Source Type: research

Use of a Neonatal-Mouse Model to Characterize Vaccines and Strategies for Overcoming the High Susceptibility and Severity of Pertussis in Early Life
Newborns and unvaccinated infants, compared to other age groups, are more susceptible to pertussis infection, manifesting severe symptoms leading to a higher mortality. The recent increase in pertussis cases demands more effective strategies to overcome this major health problem. In parallel with maternal-immunization, neonatal-immunization (NI) is a strategy needing revision. Here, using the intranasal-challenge-mouse-model we evaluated the protective capacity of NI in both naïve-mice and those with maternally acquired immunity. We tested our acellular-vaccine-candidate based on outer-membrane-vesicles derived from B...
Source: Frontiers in Microbiology - April 17, 2020 Category: Microbiology Source Type: research

Pertussis in Lao PDR: Seroprevalence and disease
Whooping cough or pertussis, a highly infectious disease that mainly occurs in children, is caused by the gram-negative bacteria Bordetella pertussis. Symptoms such as fever, runny nose and cough, occur within seven to ten days after exposure and develop into a paroxysmal cough followed by whooping (Guiso et al. 2011; World Health Organization 2015). Symptoms are often uncharacteristic, including apnoea, cyanosis and lymphocytosis and may not be recognized early on. Complications include pneumonia, otitis media, encephalitis or convulsions. (Source: International Journal of Infectious Diseases)
Source: International Journal of Infectious Diseases - April 8, 2020 Category: Infectious Diseases Authors: Daria Kleine, Somxay Billamay, Phetsavanh Chanthavilay, Sodaly Mongkhoune, Chirapha Keokhamphoui, Chanthala Souksakhone, Phonethipsavanh Nouanthong, Bouaphan Khamphaphongphane, Claude P. Muller, Antony P. Black Source Type: research

Emergence of pertactin-deficient pertussis strains in Australia can be explained by models of vaccine escape
In this study, we considered a deterministic 2-strain compartmental model to characterize the relative fitness of PRN– strains and vaccine efficacy against PRN– infection in comparison to the wild-type pertactin-expressing (PRN+) strains. We first showed that the model’s equilibrium behavior allows for replacement and co-existence, depending on key parameters related to transmission, vaccine efficacy and durations of immunity. We then fitted the model to epidemiological and pathogen PRN data from the state of New South Wales, Australia.Fitted model parameters showed that the changes in pertussis epidemiol...
Source: Epidemics - February 10, 2020 Category: Epidemiology Source Type: research

A highly sensitive one-tube nested quantitative Real-Time PCR assay for specific detection of Bordetella pertussis using the LNA technique
(Source: International Journal of Infectious Diseases)
Source: International Journal of Infectious Diseases - February 8, 2020 Category: Infectious Diseases Authors: Rui-qing Zhang, Zheng Li, Gui-xia Li, Yan-qing Tie, Xin-na Li, Yuan Gao, Qing-xia Duan, Le Wang, Li Zhao, Guo-hao Fan, Xue-ding Bai, Rui-huan Wang, Zi-wei Chen, Jin-rong Wang, Yong Wu, Meng-chuan Zhao, Zhi-shan Feng, Ji Wang, Xue-jun Ma Source Type: research

[Editors' Choice] Livening up pertussis vaccine development
Vaccination with a Bordetella pertussis live attenuated vaccine induces a beneficial TH1 cell bias and broadly targeting functional antibodies in humans. (Source: Science Translational Medicine)
Source: Science Translational Medicine - February 5, 2020 Category: Biomedical Science Authors: Boyle, M. Tags: Editors ' Choice Source Type: research

Comparative Clinical Evaluation of NeoPlex RB-8 with Seeplex PneumoBacter ACE for Simultaneous Detection of Eight Respiratory Bacterial Pathogens [Bacteriology]
This study compared the clinical concordance of NeoPlex RB-8 with another method, Seeplex PneumoBacter ACE detection assay (Seeplex PB ACE), which simultaneously detects S. pneumoniae, M. pneumoniae, C. pneumoniae, L. pneumophila, H. influenzae, and B. pertussis. We tested 2,137 nasopharyngeal swab and sputum specimens using both assays. For discordant Bordetella parapertussis and M. catarrhalis specimens, we also performed bidirectional sequencing. For S. pneumoniae, M. pneumoniae, C. pneumoniae, L. pneumophila, H. influenzae, and B. pertussis, which are detected by both NeoPlex RB-8 and Seeplex PB ACE, the positive and n...
Source: Journal of Clinical Microbiology - January 28, 2020 Category: Microbiology Authors: Kim, J. W., Hong, S. S., Lee, I. S., Chi, H. Y., Kim, S.-O., Kim, H. N., Hong, S. P. Tags: Bacteriology Source Type: research

Efficient production of cyclic adenosine monophosphate from adenosine triphosphate by the N-terminal half of adenylate cyclase from Escherichia coli
In this study, we aimed at developing an efficient biocatalytic process for bio-production of cyclic adenosine monophosphate (cAMP) from adenosine triphosphate (ATP). First, adenylate cyclase from Escherichia coli MG1655 (EAC) and Bordetella Pertussis (BAC) were expressed in E. coli BL21(DE3) and comparatively analyzed for their activities. As a result, EAC from E. coli MG1655 exhibited a higher activity. However, amount of EAC were obtained in an insoluble form. Therefore, we expressed the first 446 amino acids of EAC (EAC446) to avoid the inclusion body. The effects of induction temperature, incubation time, and incubati...
Source: Chinese Journal of Chemical Engineering - January 23, 2020 Category: Chemistry Source Type: research

High prevalence of Bordetella pertussis in young hospitalized infants with acute respiratory infection in the south of China: age- and season-dependent effects
With great interest we have read the report of Zhang et al. concerning the increased susceptibility to pertussis in Chinese adults at childbearing age, as determined in a comparative seroprevalence study using samples collected from 2010 to 2016.1 The authors describe that about 5% of the individuals had PT-IgG antibodies, which is indicative of a recent infection. In the adults 20 –39 years of age, 29.1% subjects had undetectable PT-IgG antibodies in 2010 but 57.4% in 2015/2016. It is well known that adolescents and adults have become the reservoir of pertussis and an important source of transmission to vulnerable i...
Source: Journal of Infection - January 22, 2020 Category: Infectious Diseases Authors: Heping Wang, Yuejie Zheng, Ronald de Groot, Yonghong Yang, Dimitri A. Diavatopoulos, Yunsheng Chen, Marien I. de Jonge, Jikui Deng Source Type: research

Chemical Synthesis and Immunological Evaluation of Pertussis-Like Pentasaccharide Bearing Multiple Rare Sugars as a Potential Anti-pertussis Vaccine.
Abstract The 60-year high Bordetella pertussis ( B. pertussis ) infection rate in the world suggests the urgent need for new anti-pertussis vaccines. Lipopolysaccharide (LPS) of B. pertussis is an attractive antigen for vaccine development. With the presence of multiple rare sugars and unusual glycosyl linkages, B. pertussis LPS is a highly challenging synthetic target. In this work, aided by molecular dynamics simulation and modeling, the pertussis LPS like pentasaccharide was chemically synthesized for the first time. The pentasaccharide was conjugated with a powerful carrier, bacteriophage Qβ as a vaccine ...
Source: Angewandte Chemie - January 17, 2020 Category: Chemistry Authors: Wang P, Huo CX, Lang S, Caution K, Nick ST, Dubey P, Deora R, Huang X Tags: Angew Chem Int Ed Engl Source Type: research

1146: Seroprevalence of Bordetella pertussis antibodies and anti- pertussis antibody response after Tdap vaccine in pregnant women
The primary aim was to determine the seroprevalence of anti-pertussis toxin antibodies (anti-PT IgG) among Thai pregnant women. The secondary aims were to evaluate antibodies response after tetanus toxoid, reduced diphtheria toxoid, and acellular pertussis (Tdap) vaccination between seronegative and seropositive mothers and to compare the different antibody titers at delivery among seropositive mothers who received Tdap to those who received tetanus-diphtheria vaccine (Td). (Source: American Journal of Obstetrics and Gynecology)
Source: American Journal of Obstetrics and Gynecology - December 31, 2019 Category: OBGYN Authors: Sanitra Anuwutnavin, Nalat Sompagdee, Wanatpreeya Phongsamart, Sansnee Senawong, Pinklow Umrod, Amporn Robkhonburi Tags: Poster Session V Source Type: research

[Impact of whooping cough vaccine during pregnancy on the resurgence of the disease and its form of presentation in paediatric emergency departments].
PMID: 31889662 [PubMed - as supplied by publisher] (Source: Anales de Pediatria)
Source: Anales de Pediatria - December 27, 2019 Category: Pediatrics Authors: Gangoiti I, Martinez-Fernandez E, Garmendia O, Diez A, Mintegi S Tags: An Pediatr (Barc) Source Type: research

Incidence of pertussis in the province of Pesaro-Urbino (Italy).
Authors: Brindicci G, Loconsole D, Carboni D, Genga R, Moschini E, Montorzi G, Viscogliosi F, Pompili M, Agostini M, Ripanti G Abstract Pertussis vaccination coverage in the Marche region is one of the lowest in Italy, with the province of Pesaro-Urbino remaining stable below 95% coverage since at least 2013. In this paper, we retrospectively analyzed all whooping cough notification cards arriving at the prevention department of the Area Vasta 1 Health Office in the Marche region and relating to the Pesaro-Urbino province (Italy). Between 2012 and 2017, there were 28 reported cases of pertussis with a peak in 2016 ...
Source: Infezioni in Medicina - December 19, 2019 Category: Infectious Diseases Tags: Infez Med Source Type: research

Selective protection of murine cerebral G i/o -proteins from inactivation by parenterally injected pertussis toxin
AbstractPertussis toxin (PTX) is a potent virulence factor in patients suffering from whooping cough, but in its detoxified version, it is applied for vaccination. It is thought to contribute to the pathology of the disease including various CNS malfunctions. Based on its enzymatic activity, PTX disrupts GPCR-dependent signaling by modifying the α-subunit of heterotrimeric Gi/o-proteins. It is also extensively used as a research tool to study neuronal functions in vivo and in vitro. However, data demonstrating the penetration of PTX from the blood into the brain are missing. Here, we examined the G αi/o-modifyi...
Source: Journal of Molecular Medicine - December 6, 2019 Category: Molecular Biology Source Type: research

Structural and Functional Characterization of the Globin-Coupled Sensors of Azotobacter vinelandii and Bordetella pertussis
Antioxidants&Redox Signaling, Ahead of Print. (Source: Antioxidants and Redox Signaling)
Source: Antioxidants and Redox Signaling - December 5, 2019 Category: Research Authors: Francesca Germani Marco Nardini Amy De Schutter Bert Cuypers Herald Berghmans Marie-Louise Van Hauwaert Stefano Bruno Andrea Mozzarelli Luc Moens Sabine Van Doorslaer Martino Bolognesi Alessandra Pesce Sylvia Dewilde Source Type: research

Engineering of an Iranian Bordetella pertussis strain producing inactive pertussis toxin.
Conclusion. A B. pertussis of the predominant genomic and virulence lineage in Iran was successfully engineered to produce inactive PTX. This attenuated strain will be useful to further studies to develop both whole cell and acellular pertussis vaccines. PMID: 31778110 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 28, 2019 Category: Microbiology Authors: Nikbin VS, Keramati M, Noofeli M, Tayebzadeh F, Kahali B, Shahcheraghi F Tags: J Med Microbiol Source Type: research

Human bocavirus in children hospitalized for acute respiratory tract infection in Rome.
CONCLUSIONS: HBoV can be detected alone and in co-infection respiratory samples of children with an acute respiratory tract infection. A cause-effect relationship between HBoV and respiratory infection is not clear, so further studies are needed to clarify this point. PMID: 31776891 [PubMed - as supplied by publisher] (Source: World Journal of Pediatrics : WJP)
Source: World Journal of Pediatrics : WJP - November 27, 2019 Category: Pediatrics Authors: Petrarca L, Nenna R, Frassanito A, Pierangeli A, Di Mattia G, Scagnolari C, Midulla F Tags: World J Pediatr Source Type: research

Early Protection against Pertussis Induced by Live Attenuated Bordetella pertussis BPZE1 Depends on TLR4.
In this study, we show that BPZE1 induces protection in mice against B. pertussis within days after vaccination, at a time when Ab and T cell responses were not detectable. Early protection was independent of T and B cell responses, as demonstrated by the use of SCID mice. Instead, it was due to TLR4-dependent signaling through the MyD88-dependent pathway of the innate immune response, as demonstrated in experiments with TLR4-deficient and MyD88-knockout mice. TLR2-dependent signaling did not play a major role in early protection. In addition, this study also shows that even at high doses, BPZE1 is safe in the severely imm...
Source: Journal of Immunology - November 15, 2019 Category: Allergy & Immunology Authors: Debrie AS, Mielcarek N, Lecher S, Roux X, Sirard JC, Locht C Tags: J Immunol Source Type: research

Surfaceome analysis of Australian epidemic Bordetella pertussis reveals potential vaccine antigens.
This study established surface shaving in B. pertussis, confirmed key expression differences and identified unknown surface proteins which may be potential vaccine antigens. PMID: 31703933 [PubMed - as supplied by publisher] (Source: Vaccine)
Source: Vaccine - November 5, 2019 Category: Allergy & Immunology Authors: Luu LDW, Octavia S, Aitken C, Zhong L, Raftery MJ, Sintchenko V, Lan R Tags: Vaccine Source Type: research

Quantifying outcome misclassification in multi-database studies: The case study of pertussis in the ADVANCE project.
CONCLUSION: Retrieving BorPer cases using only specific concepts has low sensitivity in PC databases, while including cases retrieved by unspecified concepts introduces false positives, which were approximately estimated to be 28% in one database. The share of cases that cannot be retrieved from a PC database because they are only seen in hospital was approximately estimated to be 15% in one database. This study demonstrated that quantifying the impact of different event-finding algorithms across databases and benchmarking with disease surveillance data can provide approximate estimates of algorithm validity. PMID: 31...
Source: Vaccine - October 31, 2019 Category: Allergy & Immunology Authors: Gini R, Dodd CN, Bollaerts K, Bartolini C, Roberto G, Huerta-Alvarez C, Martín-Merino E, Duarte-Salles T, Picelli G, Tramontan L, Danieli G, Correa A, McGee C, Becker BFH, Switzer C, Gandhi-Banga S, Bauwens J, van der Maas NAT, Spiteri G, Sdona E, Weibel Tags: Vaccine Source Type: research

High Prevalence of Macrolide-Resistant Bordetella Pertussis and ptxP1 Genotype, Mainland China, 2014 –2016
L. Li et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - October 28, 2019 Category: Infectious Diseases Source Type: research

Hfq modulates global protein pattern and stress response in Bordetella pertussis.
In this study, we used gel-free nanoLC-MS/MS-based proteomics to investigate whether Hfq, a highly conserved post-transcriptional regulatory protein, is involved in B. pertussis adaptation to low iron environment. To this end, we compared the protein profiles of wild type B. pertussis and its isogenic hfq deletion mutant strain under iron-replete and iron-depleted conditions. Almost of 33% of the proteins identified under iron starvation was found to be Hfq-dependent. Among them, proteins involved in oxidative stress tolerance and virulence factors that play a key role in the early steps of host colonization and bacterial ...
Source: Journal of Proteomics - October 24, 2019 Category: Biochemistry Authors: Hayes JA, Surmann K, Lamberti Y, Depke M, Dhople V, Blancá B, Ruiz E, Vecerek B, Schmidt F, Völker U, Rodriguez ME Tags: J Proteomics Source Type: research

AhR Activation by TCDD (2,3,7,8-Tetrachlorodibenzo-p-dioxin) Attenuates Pertussis Toxin-Induced Inflammatory Responses by Differential Regulation of Tregs and Th17 Cells Through Specific Targeting by microRNA
The Aryl Hydrocarbon Receptor (AhR) is a transcription factor that, when activated by ligand-binding, has been shown to regulate the immune response. Pertussis Toxin (PTX) is a virulence factor found in Bordetella pertussis, a human respiratory pathogen that causes whooping cough. PTX promotes colonization and disease promotion by triggering a heightened inflammatory response. The role of AhR in the regulation of PTX-mediated inflammation has not previously been studied. In the current study, we investigate if AhR activation by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a well characterized ligand, can attenuate PTX-media...
Source: Frontiers in Microbiology - October 18, 2019 Category: Microbiology Source Type: research

Seroprevalence of an antibody against diphtheria, tetanus, and pertussis among the elderly in Khon Kaen, Thailand.
CONCLUSIONS: In total, 97.2%, 83.5%, and 55.8% of the population had a higher antibody level than the minimal protective level for diphtheria, tetanus, and pertussis, respectively. In order to prevent an outbreak of these diseases in the future, the elderly should be administered with Tdap revaccination to provide diphtheria herd immunity in the population; this will increase cocoon phenomenon for pertussis and protect the population from tetanus-prone injury. PMID: 31627764 [PubMed - in process] (Source: Journal of Health, Population, and Nutrition)
Source: Journal of Health, Population, and Nutrition - October 18, 2019 Category: International Medicine & Public Health Authors: Chinchai T, Posuwan N, Vuthitanachot V, Wanlapakorn N, Poovorawan Y Tags: J Health Popul Nutr Source Type: research

Diphteria-tetanus-pertussis vaccine reduces specific IgE, inflammation and remodelling in an animal model of mite-induced respiratory allergy.
CONCLUSIONS: The DTPw vaccine reduced serum specific IgE, nasal and pulmonary inflammation and remodelling of the lower airways. PMID: 31630941 [PubMed - as supplied by publisher] (Source: Vaccine)
Source: Vaccine - October 17, 2019 Category: Allergy & Immunology Authors: Aun MV, Almeida FM, Saraiva-Romanholo BM, Martins MA, Kalil J, Arantes-Costa FM, Giavina-Bianchi P Tags: Vaccine Source Type: research

A novel multilocus variable-number tandem repeat analysis for Bordetella parapertussis.
Conclusion. Our novel MLVA method has high discriminatory power for genotyping human B. parapertussis. Regarding this organism, this genotyping system is a promising tool for epidemiological surveillance and investigating outbreaks. PMID: 31613204 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 15, 2019 Category: Microbiology Authors: Kamachi K, Otsuka N, Fumimoto R, Ozawa K, Yao SM, Chiang CS, Luu LDW, Lan R, Shibayama K, Watanabe M Tags: J Med Microbiol Source Type: research

Investigation of the effects of oxidative stress ‐inducing factors on culturing and productivity of Bordetella pertussis
In this study, stress was imposed by varying the percentage of dissolved oxygen (DO) and inoculum size, and by adding rotenone and hydrogen peroxide. In this study, fluorescence spectroscopy is used as a tool for measuring oxidative stress. High levels of DO during fed ‐batch operation had no detrimental effect on growth, but the specific productivity of pertactin (PRN) decreased. Cultures that were started with an inoculum size that was 10 times smaller than the control resulted in significantly less PRN as compared to controls where reduction was more signific ant in flasks as compared to bioreactors. A comparison of f...
Source: Biotechnology Progress - October 10, 2019 Category: Biotechnology Authors: Vanessa Zavatti, Hector Budman, Raymond L. Legge, Melih Tamer Tags: RESEARCH ARTICLE Source Type: research

Flow cytometry analysis of CD64, CD18, CD11a and CD11b in four children with Bordetella pertussis infection and admitted to critical care: New biomarkers?
Publication date: October 2019Source: Medicina Intensiva (English Edition), Volume 43, Issue 7Author(s): A. García-Salido, A. Serrano-González, A.M. de Azagra-Garde, M. Nieto-Moro, G.J. Melen, M. Ramírez-Orellana (Source: Medicina Intensiva)
Source: Medicina Intensiva - October 4, 2019 Category: Intensive Care Source Type: research

Comparison of the clinical and laboratory characteristics of pertussis or viral lower respiratory tract infections
Introduction: Whooping cough-like respiratory tract infections (WCLRTI) caused by factors other than theBordetella pertussis are available. Clinical picture is difficult to differentiate between theB. pertussisand viral respiratory infections.Methodology: Eighty-five patients with the diagnosis of WCLRTI were divided into 3 groups. Group 1 involved patients with pertussis shown by nasopharyngeal aspirate culture (NAC) and/or PCR. Group 2 consisted of patients who B. pertussis was not detected by NAC however, clinicians still evaluated them as potential patients of pertussis. Group 3 involved patients with the diagnosis of ...
Source: The Journal of Infection in Developing Countries - September 30, 2019 Category: Infectious Diseases Source Type: research