AhR Activation by TCDD (2,3,7,8-Tetrachlorodibenzo-p-dioxin) Attenuates Pertussis Toxin-Induced Inflammatory Responses by Differential Regulation of Tregs and Th17 Cells Through Specific Targeting by microRNA
The Aryl Hydrocarbon Receptor (AhR) is a transcription factor that, when activated by ligand-binding, has been shown to regulate the immune response. Pertussis Toxin (PTX) is a virulence factor found in Bordetella pertussis, a human respiratory pathogen that causes whooping cough. PTX promotes colonization and disease promotion by triggering a heightened inflammatory response. The role of AhR in the regulation of PTX-mediated inflammation has not previously been studied. In the current study, we investigate if AhR activation by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a well characterized ligand, can attenuate PTX-media...
Source: Frontiers in Microbiology - October 18, 2019 Category: Microbiology Source Type: research

Seroprevalence of an antibody against diphtheria, tetanus, and pertussis among the elderly in Khon Kaen, Thailand.
CONCLUSIONS: In total, 97.2%, 83.5%, and 55.8% of the population had a higher antibody level than the minimal protective level for diphtheria, tetanus, and pertussis, respectively. In order to prevent an outbreak of these diseases in the future, the elderly should be administered with Tdap revaccination to provide diphtheria herd immunity in the population; this will increase cocoon phenomenon for pertussis and protect the population from tetanus-prone injury. PMID: 31627764 [PubMed - in process] (Source: Journal of Health, Population, and Nutrition)
Source: Journal of Health, Population, and Nutrition - October 18, 2019 Category: International Medicine & Public Health Authors: Chinchai T, Posuwan N, Vuthitanachot V, Wanlapakorn N, Poovorawan Y Tags: J Health Popul Nutr Source Type: research

Diphteria-tetanus-pertussis vaccine reduces specific IgE, inflammation and remodelling in an animal model of mite-induced respiratory allergy.
CONCLUSIONS: The DTPw vaccine reduced serum specific IgE, nasal and pulmonary inflammation and remodelling of the lower airways. PMID: 31630941 [PubMed - as supplied by publisher] (Source: Vaccine)
Source: Vaccine - October 17, 2019 Category: Allergy & Immunology Authors: Aun MV, Almeida FM, Saraiva-Romanholo BM, Martins MA, Kalil J, Arantes-Costa FM, Giavina-Bianchi P Tags: Vaccine Source Type: research

A novel multilocus variable-number tandem repeat analysis for Bordetella parapertussis.
Conclusion. Our novel MLVA method has high discriminatory power for genotyping human B. parapertussis. Regarding this organism, this genotyping system is a promising tool for epidemiological surveillance and investigating outbreaks. PMID: 31613204 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 15, 2019 Category: Microbiology Authors: Kamachi K, Otsuka N, Fumimoto R, Ozawa K, Yao SM, Chiang CS, Luu LDW, Lan R, Shibayama K, Watanabe M Tags: J Med Microbiol Source Type: research

Investigation of the effects of oxidative stress ‐inducing factors on culturing and productivity of Bordetella pertussis
In this study, stress was imposed by varying the percentage of dissolved oxygen (DO) and inoculum size, and by adding rotenone and hydrogen peroxide. In this study, fluorescence spectroscopy is used as a tool for measuring oxidative stress. High levels of DO during fed ‐batch operation had no detrimental effect on growth, but the specific productivity of pertactin (PRN) decreased. Cultures that were started with an inoculum size that was 10 times smaller than the control resulted in significantly less PRN as compared to controls where reduction was more signific ant in flasks as compared to bioreactors. A comparison of f...
Source: Biotechnology Progress - October 10, 2019 Category: Biotechnology Authors: Vanessa Zavatti, Hector Budman, Raymond L. Legge, Melih Tamer Tags: RESEARCH ARTICLE Source Type: research

Flow cytometry analysis of CD64, CD18, CD11a and CD11b in four children with Bordetella pertussis infection and admitted to critical care: New biomarkers?
Publication date: October 2019Source: Medicina Intensiva (English Edition), Volume 43, Issue 7Author(s): A. García-Salido, A. Serrano-González, A.M. de Azagra-Garde, M. Nieto-Moro, G.J. Melen, M. Ramírez-Orellana (Source: Medicina Intensiva)
Source: Medicina Intensiva - October 4, 2019 Category: Intensive Care Source Type: research

Comparison of the clinical and laboratory characteristics of pertussis or viral lower respiratory tract infections
Introduction: Whooping cough-like respiratory tract infections (WCLRTI) caused by factors other than theBordetella pertussis are available. Clinical picture is difficult to differentiate between theB. pertussisand viral respiratory infections.Methodology: Eighty-five patients with the diagnosis of WCLRTI were divided into 3 groups. Group 1 involved patients with pertussis shown by nasopharyngeal aspirate culture (NAC) and/or PCR. Group 2 consisted of patients who B. pertussis was not detected by NAC however, clinicians still evaluated them as potential patients of pertussis. Group 3 involved patients with the diagnosis of ...
Source: The Journal of Infection in Developing Countries - September 30, 2019 Category: Infectious Diseases Source Type: research

Rare Detection of Bordetella pertussis Pertactin-Deficient Strains in Argentina
F. Carriquiriborde et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - September 30, 2019 Category: Infectious Diseases Source Type: research

Molecules, Vol. 24, Pages 3434: Hechtia glomerata Zucc: Phytochemistry and Activity of Its Extracts and Major Constituents Against Resistant Bacteria
acute;a del Rayo Camacho-Corona Hechtia glomerata Zucc. is used both as a source of food and in ethnomedicine to treat various diseases derived from bacterial infections such as bronchitis, laryngitis, nephritis, whooping cough, urethritis, and sepsis. There are no previous reports about its chemistry and biological activities. Therefore, the aims of this study were to identify components from organic and aqueous extracts of H. glomerata and test the extracts and major isolate compounds against resistant bacteria. Hexane, CHCl3/MeOH, and aqueous extracts were prepared and analyzed by different chromatographic technique...
Source: Molecules - September 21, 2019 Category: Chemistry Authors: Tommaso Stefani Elvira Garza-Gonz ález Ver ónica M. Rivas-Galindo Mar ía Yolanda Rios Laura Alvarez Mar ía del Rayo Camacho-Corona Tags: Article Source Type: research

Multiplex Polymerase Chain Reaction Panel for Suspected Pertussis: What About a Positive: Mycoplasma pneumoniae: Result?
Background: The use of bacterial multiplex polymerase chain reaction (PCR) in children with suspected pertussis sometimes yields unexpected positive results for Mycoplasma pneumoniae. We aimed to evaluate the clinical significance of positive M. pneumoniae results in this population. Methods: Retrospective cohort of consecutive patients with suspected pertussis tested with a bacterial multiplex PCR (including Bordetella pertussis and M. pneumoniae) between June 2015 and March 2017. Medical records were reviewed to compare demographics, clinical presentations and outcomes of patients positive for M. pneumoniae with tho...
Source: The Pediatric Infectious Disease Journal - September 20, 2019 Category: Infectious Diseases Tags: Original Studies Source Type: research

Commensal Microbes Affect Host Humoral Immunity to Bordetella pertussis Infection [Microbial Immunity and Vaccines]
As important players in the host defense system, commensal microbes and the microbiota influence multiple aspects of host physiology. Bordetella pertussis infection is highly contagious among humans. However, the roles of the microbiota in B. pertussis pathogenesis are poorly understood. Here, we show that antibiotic-mediated depletion of the microbiota results in increased susceptibility to B. pertussis infection during the early stage. The increased susceptibility was associated with a marked impairment of the systemic IgG, IgG2a, and IgG1 antibody responses to B. pertussis infection after antibiotic treatment. Furthermo...
Source: Infection and Immunity - September 19, 2019 Category: Infectious Diseases Authors: Zhang, Y., Ran, Z., Tian, M., Zhou, Y., Yang, J., Yin, J., Lu, D., Li, R., Zhong, J. Tags: Microbial Immunity and Vaccines Source Type: research

[Vaccine hesitancy: How to lift the brake?]
CONCLUSION: Anti-vaccine ideas were born with vaccines; they are abundantly spread through the Internet and social networks and can give a false impression of their basis in reality. It is time for positive action not merely a defensive approach. PMID: 31522949 [PubMed - as supplied by publisher] (Source: Revue des Maladies Respiratoires)
Source: Revue des Maladies Respiratoires - September 18, 2019 Category: Respiratory Medicine Tags: Rev Mal Respir Source Type: research

[Febrile seizures, epilepsy, migraine, diabetes, and heart disease as well as measles, chicken pox, and whooping cough in children and adolescents in Germany : Results from KiGGS Wave  2].
[Febrile seizures, epilepsy, migraine, diabetes, and heart disease as well as measles, chicken pox, and whooping cough in children and adolescents in Germany : Results from KiGGS Wave 2]. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2019 Sep 16;: Authors: Poethko-Müller C, Thamm R, Heidemann C, Busch M, Neuhauser H Abstract Trends of frequent chronic diseases and health problems, e.g. allergic diseases, have already been published based on the KiGGS Wave 2 study as part of the health monitoring of children and adolescents in Germany. The present work complements these ...
Source: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz - September 16, 2019 Category: International Medicine & Public Health Authors: Poethko-Müller C, Thamm R, Heidemann C, Busch M, Neuhauser H Tags: Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz Source Type: research

Collaborative study for the calibration of the replacement International Standard for pertussis toxin for use in histamine sensitisation and CHO cell clustering assays
Publication date: Available online 13 September 2019Source: BiologicalsAuthor(s): Alexandra Douglas-Bardsley, Catpagavalli Asokanathan, Sharon Tierney, Jason Hockley, Kevin MarkeyAbstractPertussis toxin (PT) in its detoxified form is one of the major protective antigens in vaccines against Bordetella pertussis (whooping cough). Reference preparations of native PT are required for the quality control of pertussis vaccines. Stocks of the first WHO International Standard (IS) for PT (JNIH-5) were low and a replacement was required. One candidate material was donated by a vaccine manufacturer to NIBSC. It was formulated, lyoph...
Source: Biologicals - September 15, 2019 Category: Biology Source Type: research

Collaborative study for the calibration of the replacement International Standard for pertussis toxin for use in histamine sensitisation and CHO cell clustering assays.
Abstract Pertussis toxin (PT) in its detoxified form is one of the major protective antigens in vaccines against Bordetella pertussis (whooping cough). Reference preparations of native PT are required for the quality control of pertussis vaccines. Stocks of the first WHO International Standard (IS) for PT (JNIH-5) were low and a replacement was required. One candidate material was donated by a vaccine manufacturer to NIBSC. It was formulated, lyophilised into sealed glass ampoules and coded 15/126. An international collaborative study assessed the suitability of this material to replace JNIH-5. Fourteen laboratori...
Source: Biologicals : Journal of the International Association of Biological Standardization - September 13, 2019 Category: Biotechnology Authors: Douglas-Bardsley A, Asokanathan C, Tierney S, Hockley J, Markey K Tags: Biologicals Source Type: research

Clinical characteristics, molecular epidemiology and antimicrobial susceptibility of pertussis among children in southern China.
CONCLUSIONS: These findings indicate that leukocytosis is not a sensitive indicator of pertussis. Isolates with the gene profile ptxP3/prn2 were highly circulating in Shenzhen and less resistant to macrolides, different from patterns observed in other parts of China. PMID: 31493136 [PubMed - as supplied by publisher] (Source: World Journal of Pediatrics : WJP)
Source: World Journal of Pediatrics : WJP - September 6, 2019 Category: Pediatrics Authors: Zhang JS, Wang HM, Yao KH, Liu Y, Lei YL, Deng JK, Yang YH Tags: World J Pediatr Source Type: research

Investigation of the Effects of Oxidative Stress Inducing Factors on Culturing and Productivity of Bordetella Pertussis
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - September 4, 2019 Category: Biotechnology Authors: Vanessa Zavatti, Hector Budman, Raymond L. Legge, Melih Tamer Tags: RESEARCH ARTICLE Source Type: research

Structure and membrane-targeting of a Bordetella pertussis effector N-terminal domain
Publication date: Available online 2 September 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Adi Yahalom, Geula Davidov, Sofiya Kolusheva, Hadassa Shaked, Shiran Barber-Zucker, Raz Zarivach, Jordan H. ChillAbstractBteA, a 69-kDa cytotoxic protein, is a type III secretion system (T3SS) effector in the classical Bordetella, the etiological agents of pertussis and related mammalian respiratory diseases. Like other cytotoxicity-mediating effectors, BteA uses its multifunctional N-terminal domain to target phosphatidylinositol (PI)-rich microdomains in the host membrane. Despite their structural simil...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - September 3, 2019 Category: Biochemistry Source Type: research

The Potential Role of Subclinical Bordetella pertussis Infection in Epilepsy
(Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - August 28, 2019 Category: Microbiology Source Type: research

Impact of Oxidative Stress on Protein Production by Bordetella pertussis for Vaccine Production
Publication date: Available online 23 August 2019Source: Biochemical Engineering JournalAuthor(s): Vanessa Zavatti, Hector Budman, Raymond L. Legge, Melih TamerAbstractThe response of Bordetella pertussis to induced oxidative stress during fermentation was assessed. B. pertussis is the causal agent of whooping cough in humans and purified pertactin (PRN) is used as one of the components in the production of acellular pertussis vaccines. When grown in bioreactors, bacteria adapt to environmental conditions which may include high levels of reactive oxidative species (ROS) that stress cells and can have detrimental effects on...
Source: Biochemical Engineering Journal - August 25, 2019 Category: Biochemistry Source Type: research

Functional Programming of Innate Immune Cells in Response to Bordetella pertussis Infection and Vaccination.
Authors: Gillard J, van Schuppen E, Diavatopoulos DA Abstract Despite widespread vaccination, B. pertussis remains one of the least controlled vaccine-preventable diseases. Although it is well known that acellular and whole cell pertussis vaccines induce distinct immune functionalities in memory cells, much less is known about the role of innate immunity in this process. In this review, we provide an overview of the known differences and similarities in innate receptors, innate immune cells and inflammatory signalling pathways induced by the pertussis vaccines either licensed or in development and compare this to p...
Source: Advances in Experimental Medicine and Biology - August 22, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research

The role of respiratory virus infection in suspected pertussis: A prospective study
ConclusionRespiratory virus infections are frequent in cases of clinical suspicion of pertussis and may actually exceed the prevalence of BP. Clinical/laboratory characteristics may suggest the etiology, but they are not pathognomonic, which stresses the need for respiratory virus and Bordetella pertussis research in this clinical situation. (Source: Journal of Microbiology, Immunology and Infection)
Source: Journal of Microbiology, Immunology and Infection - August 10, 2019 Category: Microbiology Source Type: research

Randomized Controlled Trial on the Effects of Morning versus Evening Primary Vaccination on Episodes of Hypoxemia and Bradycardia in Very Preterm Infants.
CONCLUSIONS: We did not identify a difference in CER between morning and evening vaccination but could confirm increased body temperatures and vaccination titers following vaccination. PMID: 31394525 [PubMed - as supplied by publisher] (Source: Neonatology)
Source: Neonatology - August 8, 2019 Category: Perinatology & Neonatology Authors: Gottlob S, Gille C, Poets CF Tags: Neonatology Source Type: research

Streptococcus pneumoniae and Haemophilus influenzae nasopharyngeal molecular detection in children with acute respiratory tract infection in SANADOR Hospital, Romania.
CONCLUSION: A definitive laboratory diagnosis of these micro-organisms can be made for invasive disease, but there are difficulties in establishing the aetiology for mucosal infection. Molecular detection tests could complement culture-based tests by strengthening their surveillance. PMID: 31389781 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 7, 2019 Category: Microbiology Authors: Giucă MC, Cîlcic C, Mihăescu G, Gavrilă A, Dinescu M, Gătej RI Tags: J Med Microbiol Source Type: research

Role of Major Toxin Virulence Factors in Pertussis Infection and Disease Pathogenesis.
Authors: Scanlon K, Skerry C, Carbonetti N Abstract Bordetella pertussis produces several toxins that affect host-pathogen interactions. Of these, the major toxins that contribute to pertussis infection and disease are pertussis toxin, adenylate cyclase toxin-hemolysin and tracheal cytotoxin. Pertussis toxin is a multi-subunit protein toxin that inhibits host G protein-coupled receptor signaling, causing a wide array of effects on the host. Adenylate cyclase toxin-hemolysin is a single polypeptide, containing an adenylate cyclase enzymatic domain coupled to a hemolysin domain, that primarily targets phagocytic cell...
Source: Advances in Experimental Medicine and Biology - August 5, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research

Clinical Findings and Management of Pertussis.
Authors: Polinori I, Esposito S Abstract Pertussis is an endemic highly infectious vaccine-preventable disease. The disease is a major cause of childhood morbidity and mortality. In the most recent years, the re-emergence of pertussis occurred, and many efforts were done to identify the possible causes. Certainly, more effective laboratory methods have a role in making the diagnosis easier. However, sub-optimal efficacy of available vaccines as well as their limited duration of protection could explain the resurgence of the disease. Many forms and clinical features of the disease, ranging from the most classical to...
Source: Advances in Experimental Medicine and Biology - August 1, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research

Molecular Epidemiology of Bordetella pertussis.
Authors: Barkoff AM, He Q Abstract Although vaccination has been effective, Bordetella pertussis is increasingly causing epidemics, especially in industrialized countries using acellular vaccines (aPs). One factor behind the increased circulation is the molecular changes on the pathogen level. After pertussis vaccinations were introduced, changes in the fimbrial (Fim) serotype of the circulating strains was observed. When bacterial typing methods improved, further changes between the vaccine and circulating strains, especially among the common virulence genes including pertussis toxin (PT) and pertactin (PRN) were ...
Source: Advances in Experimental Medicine and Biology - July 26, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research

Pertussis Vaccines and Vaccination Strategies. An Ever-Challenging Health Problem.
Authors: Cassone A Abstract Vaccines and vaccination against pertussis (whooping cough) have had one of the longest and most complex history, with alternating splendour and public disbelief, enthusiasm and concerns, overall resulting in changes in composition and replacement of vaccines, and associated vaccination strategies, including use of different vaccines in different countries, with no apparent equals for other bacterial vaccines. Of this both frustrating and exciting venue no end has been reached. In this note, I am shortly recapitulating the history of pertussis vaccines, from the inactivated, whole-cell v...
Source: Advances in Experimental Medicine and Biology - July 26, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research

The role of respiratory virus infection in suspected pertussis: A prospective study.
CONCLUSION: Respiratory virus infections are frequent in cases of clinical suspicion of pertussis and may actually exceed the prevalence of BP. Clinical/laboratory characteristics may suggest the etiology, but they are not pathognomonic, which stresses the need for respiratory virus and Bordetella pertussis research in this clinical situation. PMID: 31405786 [PubMed - as supplied by publisher] (Source: Journal of Microbiology, Immunology, and Infection)
Source: Journal of Microbiology, Immunology, and Infection - July 23, 2019 Category: Microbiology Authors: Ferronato AE, Leite D, Vieira SE Tags: J Microbiol Immunol Infect Source Type: research

Genome characteristics of Bordetella pertussis isolates from Tunisia.
The objective was to analyse genomic sequences of isolates collected during the 2014 whooping cough epidemic in Tunisia, a country where whole-cell vaccines are used. Ten Tunisian isolates and four vaccine strains were sequenced and compared to 169 isolates from countries where acellular vaccines are used. Phylogenetic analysis showed that Tunisian isolates are diverse, demonstrating a multi-strain 2014 epidemic peak, and are intermixed with those circulating in other world regions, showing inter-country transmission. Consistently, Tunisian isolates have antigen variant composition observed in other world regions. No perta...
Source: Journal of Medical Microbiology - July 22, 2019 Category: Microbiology Authors: Ben Fraj I, Bouchez V, Smaoui H, Kechrid A, Brisse S Tags: J Med Microbiol Source Type: research

How Genomics Is Changing What We Know About the Evolution and Genome of Bordetella pertussis.
Authors: Ring N, Abrahams JS, Bagby S, Preston A, MacArthur I Abstract The evolution of Bordetella pertussis from a common ancestor similar to Bordetella bronchiseptica has occurred through large-scale gene loss, inactivation and rearrangements, largely driven by the spread of insertion sequence element repeats throughout the genome. B. pertussis is widely considered to be monomorphic, and recent evolution of the B. pertussis genome appears to, at least in part, be driven by vaccine-based selection. Given the recent global resurgence of whooping cough despite the wide-spread use of vaccination, a more thorough unde...
Source: Advances in Experimental Medicine and Biology - July 21, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research

Pertussis: Identification, Prevention and Control.
Authors: Stefanelli P Abstract Pertussis is a vaccine-preventable disease. Despite the high vaccination coverage among children, pertussis is considered a re-emerging disease for which identification, prevention and control strategies need to be improved. To control pertussis it is important to maintain a high vaccination coverage to protect the age groups considered at high risk for the disease. Laboratory confirmation of Bordetella pertussis infection together with a differential diagnostic test for other Bordetellae are prerequisite for a correct and timely diagnosis of pertussis. Moreover, investigations of ant...
Source: Advances in Experimental Medicine and Biology - July 21, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research

Superior B. pertussis Specific CD4+ T-Cell Immunity Imprinted by Natural Infection.
Authors: Lambert EE, Buisman AM, van Els CACM Abstract Pertussis remains endemic in vaccinated populations due to waning of vaccine-induced immunity and insufficient interruption of transmission. Correlates of long-term protection against whooping cough remain elusive but increasing evidence from experimental models indicates that the priming of particular lineages of B. pertussis (Bp) specific CD4+ T cells is essential to control bacterial load. Critical hallmarks of these protective CD4+ T cell lineages in animals are suggested to be their differentiation profile as Th1 and Th17 cells and their tissue residency. ...
Source: Advances in Experimental Medicine and Biology - July 21, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research

Shotgun proteomic analysis of Bordetella parapertussis provides insights into the physiological response to iron starvation and potential new virulence determinants absent in Bordetella pertussis.
This study reports the first extensive proteome analysis of this bacterium. In an attempt to gain some insight into the infective phenotype, we evaluated the response of B. parapertussis to iron starvation, a critical stress the bacteria face during infection. Among other relevant findings, we observed that the adaptation to this condition involves significant changes in the abundance of two important virulence factors of this pathogen, namely, adenylate cyclase and the O-antigen. We further used the proteomic data to search for B. parapertussis proteins that are absent or classified as pseudogenes in the genome of Bordete...
Source: Journal of Proteomics - July 17, 2019 Category: Biochemistry Authors: Oviedo JM, Surmann K, Gorgojo JP, Valdez H, Dhople VM, Lamberti Y, Völker U, Rodriguez ME Tags: J Proteomics Source Type: research

The evolving nature of Bordetella pertussis in Ontario, Canada, 2009 –2017: strains with shifting genotypes and pertactin deficiency
This study examined the evolving nature of Bordetella pertussis in Ontario, Canada, by characterizing isolates for their genotypes and expression of pertactin (PRN). From 2009 to 2017, 413 B. pertussis were cultured from pertussis cases at the Public Health Ontario Laboratory. Their genotypes were determined by partial gene sequence analysis of their virulence and (or) vaccine antigens: filamentous haemagglutinin, PRN, fimbriae 3, and pertussis toxin, including the promoter region. Expression of PRN was measured by Western immunoblot. Two predominant genotypes, ST-1 and ST-2, were found throughout the study and were respon...
Source: Canadian Journal of Microbiology - July 11, 2019 Category: Microbiology Authors: Raymond S.W. Tsang Michelle Shuel Kirby Cronin Saul Deng Kathleen Whyte Alex Marchand-Austin Jennifer Ma Shelly Bolotin Natasha Crowcroft Kevin Schwartz Gary Van Domselaar Morag Graham Frances B. Jamieson Source Type: research

Whooping Cough in a Young Infant
New England Journal of Medicine,Volume 381, Issue 2, July 2019. (Source: New England Journal of Medicine)
Source: New England Journal of Medicine - July 10, 2019 Category: Internal Medicine Authors: Debra Hillier Source Type: research

A rapid and sensitive recombinase aided amplification assay incorporating competitive internal control to detect Bordetella pertussis using the DNA obtained by boiling
(Source: International Journal of Infectious Diseases)
Source: International Journal of Infectious Diseases - July 6, 2019 Category: Infectious Diseases Authors: Rui-qing Zhang, Gui-xia Li, Xin-na Li, Xin-xin Shen, Yuan Gao, Le Wang, Tao Fan, Qing-xia Duan, Ya-kun Wang, Ji Wang, Zhi-shan Feng, Xue-jun Ma Source Type: research

Pertussis Prevention: Reasons for Resurgence, and Differences in the Current Acellular Pertussis Vaccines
Pertussis is an acute respiratory disease caused by Bordetella pertussis. Due to the frequency and severity of this disease, prevention of pertussis has been considered an important public health issue for many years. The development of the whole-cell pertussis vaccine (wPV) and its introduction into the paediatric immunization schedule was associated with a marked reduction in pertussis cases in the vaccinated cohort. However, due to the frequency of local and systemic adverse events after immunization with wPV, work on a less reactive vaccine was undertaken based on B. pertussis components that induced protective immune ...
Source: Frontiers in Immunology - July 3, 2019 Category: Allergy & Immunology Source Type: research

[Vaccine rejection and vaccination management: the grey areasRecusa vacinal e gest ão da imunização: nuances e contrastes].
[Vaccine rejection and vaccination management: the grey areasRecusa vacinal e gestão da imunização: nuances e contrastes]. Rev Panam Salud Publica. 2019;43:e54 Authors: Hortal M, Di Fabio JL Abstract Vaccinating children has been an unquestioned tradition for many years. However, there is now great concern over the growing rejection of childhood vaccination, as well as other less evident obstacles that affect vaccination coverage.Multiple factors are involved in the rejection of a specific vaccine or vaccination in general, including actions by anti-vaccination groups, as well as d...
Source: Pan American Journal of Public Health - July 2, 2019 Category: International Medicine & Public Health Tags: Rev Panam Salud Publica Source Type: research

Causes of severe pneumonia requiring hospital admission in children without HIV infection from Africa and Asia: the PERCH multi-country case-control study
In this study, we estimated causes of pneumonia in young African and Asian children, using novel analytical methods applied to clinical and microbiological findings.MethodsWe did a multi-site, international case-control study in nine study sites in seven countries: Bangladesh, The Gambia, Kenya, Mali, South Africa, Thailand, and Zambia. All sites enrolled in the study for 24 months. Cases were children aged 1–59 months admitted to hospital with severe pneumonia. Controls were age-group-matched children randomly selected from communities surrounding study sites. Nasopharyngeal and oropharyngeal (NP-OP), urine, blood, ...
Source: The Lancet - June 28, 2019 Category: General Medicine Source Type: research

Clinical and epidemiological characteristics of whooping cough in hospitalized patients of a tertiary care hospital in Peru
Conclusion: Whooping cough is a cause of morbidity and mortality, especially in those younger than 6 months of age and in those who are not immunized or only partially immunized. Vaccination rates should be improved and case confirmation encouraged to prevent the underdiagnosis of this disease. (Source: Revista Brasileira de Terapia Intensiva)
Source: Revista Brasileira de Terapia Intensiva - June 27, 2019 Category: Intensive Care Source Type: research

Bordetella pertussis antigens encapsulated into N-trimethyl chitosan nanoparticulate systems as a novel intranasal pertussis vaccine.
Authors: Najminejad H, Kalantar SM, Mokarram AR, Dabaghian M, Abdollahpour-Alitappeh M, Ebrahimi SM, Tebianian M, Fasihi Ramandi M, Sheikhha MH Abstract The mucosal immune system serves as the first line of defense against Bordetella pertussis. Intranasal vaccination, due to its potential to induce systemic and mucosal immune responses, appears to prevent the initial adherence and colonization of the bacteria at the first point of contact. In the present study, two B. pertussis antigens, pertussis Toxoid (PTd) and Filamentous hemagglutinin (FHA), which play a very significant role in virulence and protection agains...
Source: Artificial Cells, Nanomedicine and Biotechnology - June 27, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Bordetella pertussis antigens encapsulated into N-trimethyl chitosan nanoparticulate systems as a novel intranasal pertussis vaccine
Volume 47, Issue 1, December 2019, Page 2605-2611 . (Source: Artificial Cells, Blood Substitutes, and Biotechnology)
Source: Artificial Cells, Blood Substitutes, and Biotechnology - June 26, 2019 Category: Biotechnology Authors: Hamid Najminejad Seyed Mehdi Kalantar Ali Rezaei Mokarram Mehran Dabaghian Meghdad Abdollahpour-Alitappeh Seyyed Mahmoud Ebrahimi Majid Tebianian Mahdi Fasihi Ramandi Mohammad Hasan Sheikhha Source Type: research

Antibody Specificity Following a Recent Bordetella pertussis Infection in Adolescence Is Correlated With the Pertussis Vaccine Received in Childhood
This study demonstrated that B. pertussis infection-induced antibody responses were distinct on antigen level between individuals with either wPV or aPV immunization background. Importantly, only 2DEWB and not MIA could detect these differences indicating the potential of this method. Moreover, in individuals immunized with an aPV containing only PTx in childhood, the infection-induced antibody responses were not limited to PTx alone. (Source: Frontiers in Immunology)
Source: Frontiers in Immunology - June 17, 2019 Category: Allergy & Immunology Source Type: research

Proteomics of Bordetella pertussis whole-cell and acellular vaccines
Bordetella pertussis is the etiological agent of whooping cough, a bacterial infection of especially children, which may be fatal without treatment. In frame of studies to investigate putative effects of vaccinat... (Source: BMC Research Notes)
Source: BMC Research Notes - June 10, 2019 Category: Research Authors: Jens M öller, Max Edmund Kraner and Andreas Burkovski Tags: Data note Source Type: research

Proteomics of Bordetella pertussis whole-cell and acellular vaccines.
i A Abstract OBJECTIVES: Bordetella pertussis is the etiological agent of whooping cough, a bacterial infection of especially children, which may be fatal without treatment. In frame of studies to investigate putative effects of vaccination on host-pathogen interaction and clonal distribution of strains, in addition to Corynebacterium diphtheriae and Clostridium tetani toxoid vaccines, also whole-cell and acellular pertussis vaccines were analyzed by mass spectrometry. DATA DESCRIPTION: LC-MS/MS spectra were generated and analyzed using B. pertussis genome data and proteins present in whole-cell and acellular...
Source: Cell Research - June 10, 2019 Category: Cytology Authors: Möller J, Kraner ME, Burkovski A Tags: BMC Res Notes Source Type: research

Genetic analysis of clinical and vaccine strains of Bordetella pertussis by Pulsed-Field Gel Electrophoresis (PFGE), Multi Locus Sequence Typing (MLST) and serotyping.
Abstract In spite of high vaccination coverage in the Expanded Program of Immunization (EPI), pertussis has not been eradicated yet and the re-emergence of the disease is still reported worldwide. The genetic divergence study of circulating clinical strains of Bordetella pertussis among the population with high vaccination coverage is a useful tool to have an insight in the understanding of genetic patterns of this bacterium and deviation of them from vaccine strains. Different methods are accessible for studying of Bordetella pertussis that can perform appropriate assessment between populations. Strains used in t...
Source: Comparative immunology, microbiology and infectious diseases. - June 1, 2019 Category: Allergy & Immunology Authors: Bahmanjeh A, Noofeli M, Khaki P, Hassanzadeh SM Tags: Comp Immunol Microbiol Infect Dis Source Type: research