Establishment of virus-induced gene-silencing system in Juglans sigillata Dode and functional analysis of JsFLS2 and JsFLS4
This study showed that photobleaching occurred 24 and 30 d after the silencing vector was infected with aseptic seedlings and fruits of J. sigillata, respectively. When the OD600 was 0.6, and the injection dose was 500 μL, the gene silencing efficiency of aseptic seedlings was the highest at 16.7 %, significantly better than other treatments. Moreover, when the OD600 was 0.8, and the injection dose was 500 μL, the gene silencing efficiency in the walnut fruit was the highest (20 %). In addition, the VIGS system was successfully used to silence JsFLS2 and JsFLS4 genes in J. sigillata. This study also showed that the flavo...
Source: Gene - March 17, 2024 Category: Genetics & Stem Cells Authors: Yanyang Hong Rong Wei Chunxiang Li Hu Cai Erjuan Chen Xuejun Pan Wen'e Zhang Source Type: research

Walnut supplementation increases levels of UCP1 and CD36 in brown adipose tissue independently of diet type
Mol Cell Biochem. 2024 Mar 13. doi: 10.1007/s11010-024-04981-7. Online ahead of print.ABSTRACTDietary interventions that modulate the brown adipose tissue (BAT) thermogenic activity could represent a promising therapy for metabolic disorders. In order to examine if dietary walnuts intake regulates the expression of BAT thermogenic markers levels in healthy and metabolically challenged (fructose fed) animals, rats were initially divided into the control and fructose-fed groups. After nine weeks, these groups were subdivided into the one kept on the original regimens and the other supplemented with walnuts. High-fructose die...
Source: Molecular and Cellular Biochemistry - March 13, 2024 Category: Biochemistry Authors: Tamara Dakic Dusan Jeremic Iva Lakic Nebojsa Jasnic Aleksandra Ruzicic Predrag Vujovic Tanja Jevdjovic Source Type: research

Antioxidant constituents from < em > Ribes glaciale < /em > Wall.: bioassay targeted < em > in-vitro < /em > and < em > in-silico < /em > evaluation
Nat Prod Res. 2024 Mar 10:1-4. doi: 10.1080/14786419.2024.2326955. Online ahead of print.ABSTRACTBioassay targeted phyto-investigation of dried green walnut husk of Ribes glaciale Wall. yielded one new compound as β-D-glucopyrano (4'→3)-β-D-glucopyranose (1) and four known compounds namely scoparone (2), apigenin (3), β-sitosterol (4) and β-sitosterol-D-glucoside (5). The structure of new compound was elucidated with the help of 1D, 2D and HRESIMS analysis. The antioxidant activity of extract, fractions and pure were evaluated using 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2, 2-diphenyl-1-pic...
Source: Natural Product Research - March 11, 2024 Category: Biochemistry Authors: Rajendra Singh Rohit Verma Nitin Sati Waseem Ahmad Ansari Mohammad Faheem Khan Source Type: research

Antioxidant constituents from < em > Ribes glaciale < /em > Wall.: bioassay targeted < em > in-vitro < /em > and < em > in-silico < /em > evaluation
Nat Prod Res. 2024 Mar 10:1-4. doi: 10.1080/14786419.2024.2326955. Online ahead of print.ABSTRACTBioassay targeted phyto-investigation of dried green walnut husk of Ribes glaciale Wall. yielded one new compound as β-D-glucopyrano (4'→3)-β-D-glucopyranose (1) and four known compounds namely scoparone (2), apigenin (3), β-sitosterol (4) and β-sitosterol-D-glucoside (5). The structure of new compound was elucidated with the help of 1D, 2D and HRESIMS analysis. The antioxidant activity of extract, fractions and pure were evaluated using 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2, 2-diphenyl-1-pic...
Source: Natural Product Research - March 11, 2024 Category: Biochemistry Authors: Rajendra Singh Rohit Verma Nitin Sati Waseem Ahmad Ansari Mohammad Faheem Khan Source Type: research

An in silico scheme for optimizing the enzymatic acquisition of natural biologically active peptides based on machine learning and virtual digestion
Anal Chim Acta. 2024 Apr 15;1298:342419. doi: 10.1016/j.aca.2024.342419. Epub 2024 Feb 26.ABSTRACTBACKGROUND: As a potential natural active substance, natural biologically active peptides (NBAPs) are recently attracting increasing attention. The traditional proteolysis methods of obtaining effective NBAPs are considerably vexing, especially since multiple proteases can be used, which blocks the exploration of available NBAPs. Although the development of virtual digesting brings some degree of convenience, the activity of the obtained peptides remains unclear, which would still not allow efficient access to the NBAPs. It is...
Source: Analytica Chimica Acta - March 10, 2024 Category: Chemistry Authors: Like Lin Cong Li Tianlong Zhang Chaoshuang Xia Qiuhong Bai Lihua Jin Yehua Shen Source Type: research

An in silico scheme for optimizing the enzymatic acquisition of natural biologically active peptides based on machine learning and virtual digestion
Anal Chim Acta. 2024 Apr 15;1298:342419. doi: 10.1016/j.aca.2024.342419. Epub 2024 Feb 26.ABSTRACTBACKGROUND: As a potential natural active substance, natural biologically active peptides (NBAPs) are recently attracting increasing attention. The traditional proteolysis methods of obtaining effective NBAPs are considerably vexing, especially since multiple proteases can be used, which blocks the exploration of available NBAPs. Although the development of virtual digesting brings some degree of convenience, the activity of the obtained peptides remains unclear, which would still not allow efficient access to the NBAPs. It is...
Source: Analytica Chimica Acta - March 10, 2024 Category: Chemistry Authors: Like Lin Cong Li Tianlong Zhang Chaoshuang Xia Qiuhong Bai Lihua Jin Yehua Shen Source Type: research

An in silico scheme for optimizing the enzymatic acquisition of natural biologically active peptides based on machine learning and virtual digestion
Anal Chim Acta. 2024 Apr 15;1298:342419. doi: 10.1016/j.aca.2024.342419. Epub 2024 Feb 26.ABSTRACTBACKGROUND: As a potential natural active substance, natural biologically active peptides (NBAPs) are recently attracting increasing attention. The traditional proteolysis methods of obtaining effective NBAPs are considerably vexing, especially since multiple proteases can be used, which blocks the exploration of available NBAPs. Although the development of virtual digesting brings some degree of convenience, the activity of the obtained peptides remains unclear, which would still not allow efficient access to the NBAPs. It is...
Source: Analytica Chimica Acta - March 10, 2024 Category: Chemistry Authors: Like Lin Cong Li Tianlong Zhang Chaoshuang Xia Qiuhong Bai Lihua Jin Yehua Shen Source Type: research

An in silico scheme for optimizing the enzymatic acquisition of natural biologically active peptides based on machine learning and virtual digestion
Anal Chim Acta. 2024 Apr 15;1298:342419. doi: 10.1016/j.aca.2024.342419. Epub 2024 Feb 26.ABSTRACTBACKGROUND: As a potential natural active substance, natural biologically active peptides (NBAPs) are recently attracting increasing attention. The traditional proteolysis methods of obtaining effective NBAPs are considerably vexing, especially since multiple proteases can be used, which blocks the exploration of available NBAPs. Although the development of virtual digesting brings some degree of convenience, the activity of the obtained peptides remains unclear, which would still not allow efficient access to the NBAPs. It is...
Source: Analytica Chimica Acta - March 10, 2024 Category: Chemistry Authors: Like Lin Cong Li Tianlong Zhang Chaoshuang Xia Qiuhong Bai Lihua Jin Yehua Shen Source Type: research

An in silico scheme for optimizing the enzymatic acquisition of natural biologically active peptides based on machine learning and virtual digestion
Anal Chim Acta. 2024 Apr 15;1298:342419. doi: 10.1016/j.aca.2024.342419. Epub 2024 Feb 26.ABSTRACTBACKGROUND: As a potential natural active substance, natural biologically active peptides (NBAPs) are recently attracting increasing attention. The traditional proteolysis methods of obtaining effective NBAPs are considerably vexing, especially since multiple proteases can be used, which blocks the exploration of available NBAPs. Although the development of virtual digesting brings some degree of convenience, the activity of the obtained peptides remains unclear, which would still not allow efficient access to the NBAPs. It is...
Source: Analytica Chimica Acta - March 10, 2024 Category: Chemistry Authors: Like Lin Cong Li Tianlong Zhang Chaoshuang Xia Qiuhong Bai Lihua Jin Yehua Shen Source Type: research

An in silico scheme for optimizing the enzymatic acquisition of natural biologically active peptides based on machine learning and virtual digestion
Anal Chim Acta. 2024 Apr 15;1298:342419. doi: 10.1016/j.aca.2024.342419. Epub 2024 Feb 26.ABSTRACTBACKGROUND: As a potential natural active substance, natural biologically active peptides (NBAPs) are recently attracting increasing attention. The traditional proteolysis methods of obtaining effective NBAPs are considerably vexing, especially since multiple proteases can be used, which blocks the exploration of available NBAPs. Although the development of virtual digesting brings some degree of convenience, the activity of the obtained peptides remains unclear, which would still not allow efficient access to the NBAPs. It is...
Source: Analytica Chimica Acta - March 10, 2024 Category: Chemistry Authors: Like Lin Cong Li Tianlong Zhang Chaoshuang Xia Qiuhong Bai Lihua Jin Yehua Shen Source Type: research

Concurrent training associated with moderate walnut consumption improved isokinetic strength, subjective sleep quality, cognitive performance and postural balance in elderly active men: a randomized controlled trial
CONCLUSIONS: The present study clearly revealed that concurrent training alone or associated with daily walnut (15 g) consumption for 6 weeks significantly increased knee isokinetic strength, support leg standing parameters and sleep quality. Meanwhile, cognitive performance evaluated by MoCA test and postural balance with dual task were improved for CTW group only.PMID:38421528 | DOI:10.1007/s40520-023-02646-x (Source: Aging Clinical and Experimental Research)
Source: Aging Clinical and Experimental Research - February 29, 2024 Category: Geriatrics Authors: Anis Kamoun Abdelmonem Yahia Mohamed Amine Farjallah Rami Maaloul Houssem Marzougui Mohamed Bouaziz Nizar Souissi Mohamed Habib Elleuch Omar Hammouda Source Type: research

Concurrent training associated with moderate walnut consumption improved isokinetic strength, subjective sleep quality, cognitive performance and postural balance in elderly active men: a randomized controlled trial
CONCLUSIONS: The present study clearly revealed that concurrent training alone or associated with daily walnut (15 g) consumption for 6 weeks significantly increased knee isokinetic strength, support leg standing parameters and sleep quality. Meanwhile, cognitive performance evaluated by MoCA test and postural balance with dual task were improved for CTW group only.PMID:38421528 | DOI:10.1007/s40520-023-02646-x (Source: Aging Clinical and Experimental Research)
Source: Aging Clinical and Experimental Research - February 29, 2024 Category: Geriatrics Authors: Anis Kamoun Abdelmonem Yahia Mohamed Amine Farjallah Rami Maaloul Houssem Marzougui Mohamed Bouaziz Nizar Souissi Mohamed Habib Elleuch Omar Hammouda Source Type: research

Concurrent training associated with moderate walnut consumption improved isokinetic strength, subjective sleep quality, cognitive performance and postural balance in elderly active men: a randomized controlled trial
CONCLUSIONS: The present study clearly revealed that concurrent training alone or associated with daily walnut (15 g) consumption for 6 weeks significantly increased knee isokinetic strength, support leg standing parameters and sleep quality. Meanwhile, cognitive performance evaluated by MoCA test and postural balance with dual task were improved for CTW group only.PMID:38421528 | PMC:PMC10904559 | DOI:10.1007/s40520-023-02646-x (Source: Aging Clinical and Experimental Research)
Source: Aging Clinical and Experimental Research - February 29, 2024 Category: Geriatrics Authors: Anis Kamoun Abdelmonem Yahia Mohamed Amine Farjallah Rami Maaloul Houssem Marzougui Mohamed Bouaziz Nizar Souissi Mohamed Habib Elleuch Omar Hammouda Source Type: research

Concurrent training associated with moderate walnut consumption improved isokinetic strength, subjective sleep quality, cognitive performance and postural balance in elderly active men: a randomized controlled trial
CONCLUSIONS: The present study clearly revealed that concurrent training alone or associated with daily walnut (15 g) consumption for 6 weeks significantly increased knee isokinetic strength, support leg standing parameters and sleep quality. Meanwhile, cognitive performance evaluated by MoCA test and postural balance with dual task were improved for CTW group only.PMID:38421528 | PMC:PMC10904559 | DOI:10.1007/s40520-023-02646-x (Source: Aging Clinical and Experimental Research)
Source: Aging Clinical and Experimental Research - February 29, 2024 Category: Geriatrics Authors: Anis Kamoun Abdelmonem Yahia Mohamed Amine Farjallah Rami Maaloul Houssem Marzougui Mohamed Bouaziz Nizar Souissi Mohamed Habib Elleuch Omar Hammouda Source Type: research

Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
CONCLUSION: Our results suggest that Juglone is a potential anticancer agent especially when combined with ascorbate.PMID:38393422 | DOI:10.1007/s11033-024-09254-6 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - February 23, 2024 Category: Molecular Biology Authors: Dudu Erkoc-Kaya Hilal Arikoglu Ebru Guclu Duygu Dursunoglu Esma Menevse Source Type: research