Pyridoxal kinase gene deletion leads to impaired growth, deranged redox metabolism and cell cycle arrest in Leishmania donovani
Biochimie. 2024 Feb 23:S0300-9084(24)00047-6. doi: 10.1016/j.biochi.2024.02.009. Online ahead of print.ABSTRACTPyridoxal kinase (PdxK) is a vitamin B6 salvage pathway enzyme which produces pyridoxal phosphate. We have investigated the impact of PdxK deletion in Leishmania donovani on parasite survivability, infectivity and cellular metabolism. LdPdxK mutants were generated by gene replacement strategy. All mutants showed significant reduction in growth in comparison to wild type. For PdxK mediated biochemical perturbations, only heterozygous mutants and complementation mutants were used as the growth of null mutants were c...
Source: Biochimie - February 25, 2024 Category: Biochemistry Authors: Pradyot Kumar Roy Anindita Paul Sandra Lalchhuanawmi Neerupudi Kishore Babu Sushma Singh Source Type: research

The MMACHC variant c.158T & gt;C: Mild clinical and biochemical phenotypes and marked hydroxocobalamin response in cblC patients
Mol Genet Metab. 2024 Feb 10;142(1):108345. doi: 10.1016/j.ymgme.2024.108345. Online ahead of print.ABSTRACTMutations in MMACHC cause cobalamin C disease (cblC, OMIM 277400), the commonest inborn error of vitamin B12 metabolism. In cblC, deficient activation of cobalamin results in methylcobalamin and adenosylcobalamin deficiency, elevating methylmalonic acid (MMA) and total plasma homocysteine (tHcy). We retrospectively reviewed the medical files of seven cblC patients: three compound heterozygotes for the MMACHC (NM_015506.3) missense variant c.158T>C p.(Leu53Pro) in trans with the common pathogenic mutation c.271dupA...
Source: Molecular Medicine - February 22, 2024 Category: Molecular Biology Authors: Tanguy Demaret Karine B édard Jean-Fran çois Soucy David Watkins Pierre Allard Alina Levtova Alan O'Brien Catherine Brunel-Guitton David S Rosenblatt Grant A Mitchell Source Type: research

Wireless and battery-free wearable biosensing of riboflavin in sweat for precision nutrition
Biosens Bioelectron. 2024 Feb 17;251:116136. doi: 10.1016/j.bios.2024.116136. Online ahead of print.ABSTRACTNutrition assessment is crucial for dietary guidance and prevention of malnutrition. Recent endeavors in wearable biochemical sensors have enabled real-time, in situ analysis of nutrients in sweat. However, the monitoring of riboflavin, an indispensable vitamin B involved in energy metabolism, remains challenging due to its trace level and variations in the sweat matrix. Herein, we report a wireless, battery-free, and flexible wearable biosensing system for the in situ monitoring of sweat riboflavin. Highly sensitive...
Source: Biosensors and Bioelectronics - February 20, 2024 Category: Biotechnology Authors: Zhenghan Shi Peixue Deng Li-Ang Zhou Meng Jin Feiyue Fang Tao Chen Guang Liu Hao Wen Zijian An Hao Liang Yanli Lu Jun Liu Qingjun Liu Source Type: research

Temporal Changes in Micronutrient Intake Among U.S. Adults, NHANES 2003 through 2018: A Cross-Sectional Study
CONCLUSIONS: Although some progress has been made, nutrient intake and percentage of the population meeting nutritional recommendations among U.S. adults has changed little over the 15 years studied.PMID:38373695 | DOI:10.1016/j.ajcnut.2024.02.007 (Source: The American Journal of Clinical Nutrition)
Source: The American Journal of Clinical Nutrition - February 19, 2024 Category: Nutrition Authors: Marjorie R Freedman Victor L Fulgoni Harris R Lieberman Source Type: research

Temporal Changes in Micronutrient Intake Among U.S. Adults, NHANES 2003 through 2018: A Cross-Sectional Study
CONCLUSIONS: Although some progress has been made, nutrient intake and percentage of the population meeting nutritional recommendations among U.S. adults has changed little over the 15 years studied.PMID:38373695 | DOI:10.1016/j.ajcnut.2024.02.007 (Source: Am J Clin Nutr)
Source: Am J Clin Nutr - February 19, 2024 Category: Nutrition Authors: Marjorie R Freedman Victor L Fulgoni Harris R Lieberman Source Type: research

Temporal Changes in Micronutrient Intake Among U.S. Adults, NHANES 2003 through 2018: A Cross-Sectional Study
CONCLUSIONS: Although some progress has been made, nutrient intake and percentage of the population meeting nutritional recommendations among U.S. adults has changed little over the 15 years studied.PMID:38373695 | DOI:10.1016/j.ajcnut.2024.02.007 (Source: The American Journal of Clinical Nutrition)
Source: The American Journal of Clinical Nutrition - February 19, 2024 Category: Nutrition Authors: Marjorie R Freedman Victor L Fulgoni Harris R Lieberman Source Type: research

Temporal Changes in Micronutrient Intake Among U.S. Adults, NHANES 2003 through 2018: A Cross-Sectional Study
CONCLUSIONS: Although some progress has been made, nutrient intake and percentage of the population meeting nutritional recommendations among U.S. adults has changed little over the 15 years studied.PMID:38373695 | DOI:10.1016/j.ajcnut.2024.02.007 (Source: Am J Clin Nutr)
Source: Am J Clin Nutr - February 19, 2024 Category: Nutrition Authors: Marjorie R Freedman Victor L Fulgoni Harris R Lieberman Source Type: research

Temporal Changes in Micronutrient Intake Among U.S. Adults, NHANES 2003 through 2018: A Cross-Sectional Study
CONCLUSIONS: Although some progress has been made, nutrient intake and percentage of the population meeting nutritional recommendations among U.S. adults has changed little over the 15 years studied.PMID:38373695 | DOI:10.1016/j.ajcnut.2024.02.007 (Source: The American Journal of Clinical Nutrition)
Source: The American Journal of Clinical Nutrition - February 19, 2024 Category: Nutrition Authors: Marjorie R Freedman Victor L Fulgoni Harris R Lieberman Source Type: research

Temporal Changes in Micronutrient Intake Among U.S. Adults, NHANES 2003 through 2018: A Cross-Sectional Study
CONCLUSIONS: Although some progress has been made, nutrient intake and percentage of the population meeting nutritional recommendations among U.S. adults has changed little over the 15 years studied.PMID:38373695 | DOI:10.1016/j.ajcnut.2024.02.007 (Source: Am J Clin Nutr)
Source: Am J Clin Nutr - February 19, 2024 Category: Nutrition Authors: Marjorie R Freedman Victor L Fulgoni Harris R Lieberman Source Type: research

Temporal Changes in Micronutrient Intake Among U.S. Adults, NHANES 2003 through 2018: A Cross-Sectional Study
CONCLUSIONS: Although some progress has been made, nutrient intake and percentage of the population meeting nutritional recommendations among U.S. adults has changed little over the 15 years studied.PMID:38373695 | DOI:10.1016/j.ajcnut.2024.02.007 (Source: The American Journal of Clinical Nutrition)
Source: The American Journal of Clinical Nutrition - February 19, 2024 Category: Nutrition Authors: Marjorie R Freedman Victor L Fulgoni Harris R Lieberman Source Type: research

Temporal Changes in Micronutrient Intake Among U.S. Adults, NHANES 2003 through 2018: A Cross-Sectional Study
CONCLUSIONS: Although some progress has been made, nutrient intake and percentage of the population meeting nutritional recommendations among U.S. adults has changed little over the 15 years studied.PMID:38373695 | DOI:10.1016/j.ajcnut.2024.02.007 (Source: Am J Clin Nutr)
Source: Am J Clin Nutr - February 19, 2024 Category: Nutrition Authors: Marjorie R Freedman Victor L Fulgoni Harris R Lieberman Source Type: research

Temporal Changes in Micronutrient Intake Among U.S. Adults, NHANES 2003 through 2018: A Cross-Sectional Study
CONCLUSIONS: Although some progress has been made, nutrient intake and percentage of the population meeting nutritional recommendations among U.S. adults has changed little over the 15 years studied.PMID:38373695 | DOI:10.1016/j.ajcnut.2024.02.007 (Source: Am J Clin Nutr)
Source: Am J Clin Nutr - February 19, 2024 Category: Nutrition Authors: Marjorie R Freedman Victor L Fulgoni Harris R Lieberman Source Type: research

Temporal Changes in Micronutrient Intake Among U.S. Adults, NHANES 2003 through 2018: A Cross-Sectional Study
CONCLUSIONS: Although some progress has been made, nutrient intake and percentage of the population meeting nutritional recommendations among U.S. adults has changed little over the 15 years studied.PMID:38373695 | DOI:10.1016/j.ajcnut.2024.02.007 (Source: The American Journal of Clinical Nutrition)
Source: The American Journal of Clinical Nutrition - February 19, 2024 Category: Nutrition Authors: Marjorie R Freedman Victor L Fulgoni Harris R Lieberman Source Type: research

Thiopseudomonas acetoxidans sp. nov., an aerobic acetic and butyric acids oxidizer isolated from anaerobic fermentation liquid of food waste
Antonie Van Leeuwenhoek. 2024 Feb 13;117(1):35. doi: 10.1007/s10482-024-01932-6.ABSTRACTA Gram-stain-negative, oxidase-negative, rod-shaped, motile, facultatively anaerobic bacterial strain, designated as CY1220T, was isolated from an anaerobic fermentation liquid of food waste treatment plant. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain CY1220T belongs to the genus Thiopseudomonas, with the highest sequence similarity to Thiopseudomonas alkaliphila B4199T (95.91%), followed by Thiopseudomonas denitrificans X2T (95.56%). The genomic DNA G + C content of strain CY1220T was 48.6 mol%. The...
Source: Antonie van Leeuwenhoek - February 13, 2024 Category: Microbiology Authors: Miaomiao An Ruina Liang Yanjuan Lu Xiaoxu Li Guozhu Zhao Source Type: research

Thiopseudomonas acetoxidans sp. nov., an aerobic acetic and butyric acids oxidizer isolated from anaerobic fermentation liquid of food waste
Antonie Van Leeuwenhoek. 2024 Feb 13;117(1):35. doi: 10.1007/s10482-024-01932-6.ABSTRACTA Gram-stain-negative, oxidase-negative, rod-shaped, motile, facultatively anaerobic bacterial strain, designated as CY1220T, was isolated from an anaerobic fermentation liquid of food waste treatment plant. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain CY1220T belongs to the genus Thiopseudomonas, with the highest sequence similarity to Thiopseudomonas alkaliphila B4199T (95.91%), followed by Thiopseudomonas denitrificans X2T (95.56%). The genomic DNA G + C content of strain CY1220T was 48.6 mol%. The...
Source: Antonie van Leeuwenhoek - February 13, 2024 Category: Microbiology Authors: Miaomiao An Ruina Liang Yanjuan Lu Xiaoxu Li Guozhu Zhao Source Type: research