Optimizing retinal ganglion cell nuclear staining for automated cell counting
Exp Eye Res. 2024 Mar 28:109881. doi: 10.1016/j.exer.2024.109881. Online ahead of print.ABSTRACTThe retinal ganglion cells (RGCs) serve as the critical pathway for transmitting visual information from the retina to the brain, yet they can be dramatically impacted by diseases such as glaucoma. When investigating disease processes affecting RGCs in mouse models, accurately quantifying affected cells becomes essential. However, the use of pan RGC markers like RBPMS or THY1 presents challenges in accurate total cell counting. While Brn3a serves as a reliable RGC nuclear marker for automated counting, it fails to encompass all ...
Source: Experimental Eye Research - March 30, 2024 Category: Opthalmology Authors: Fangyu Lin Su-Ting Lin Jiaxing Wang Eldon E Geisert Source Type: research
Transcriptomics confirms IRF1 as a key regulator of pyroptosis in diabetic retinopathy
This study aimed to establish an in vitro model of diabetic retinopathy by culturing human retinal endothelial cells (HREC) with high glucose (30 mmol/L), and to detect their transcriptome expression by RNA-seq, screen for key genes related to pyroptosis, and validate the sequencing results by subsequent experiments.METHODS: We used RNA-seq to detect the transcriptome expression differences between HREC cells cultured with high glucose and control group, and identified differentially expressed genes by GO/KEGG analysis. We constructed a PPI network and determined the key genes by Cytoscape software and CytoHubba plugin. We...
Source: Biochemical and Biophysical Research communications - March 30, 2024 Category: Biochemistry Authors: Yang Xian Xingli Wang Yong Yu XiaoLong Chen Source Type: research
Optimizing retinal ganglion cell nuclear staining for automated cell counting
Exp Eye Res. 2024 Mar 28:109881. doi: 10.1016/j.exer.2024.109881. Online ahead of print.ABSTRACTThe retinal ganglion cells (RGCs) serve as the critical pathway for transmitting visual information from the retina to the brain, yet they can be dramatically impacted by diseases such as glaucoma. When investigating disease processes affecting RGCs in mouse models, accurately quantifying affected cells becomes essential. However, the use of pan RGC markers like RBPMS or THY1 presents challenges in accurate total cell counting. While Brn3a serves as a reliable RGC nuclear marker for automated counting, it fails to encompass all ...
Source: Experimental Eye Research - March 30, 2024 Category: Opthalmology Authors: Fangyu Lin Su-Ting Lin Jiaxing Wang Eldon E Geisert Source Type: research
Dancing in the eye: dynamic optical coherence tomography to distinguish different retinal microglia populations
(Source: International Ophthalmology)
Source: International Ophthalmology - March 30, 2024 Category: Opthalmology Source Type: research
A prospective study of smoking-related white blood cell DNA methylation markers and risk of bladder cancer
AbstractBladder cancer, a common neoplasm, is primarily caused by tobacco smoking. Epigenetic alterations including DNA methylation have the potential to be used as prospective markers of increased risk, particularly in at-risk populations such as smokers. We aimed to investigate the potential of smoking-related white blood cell (WBC) methylation markers to contribute to an increase in bladder cancer risk prediction over classical questionnaire-based smoking metrics (i.e., duration, intensity, packyears) in a nested case –control study within the prospective prostate, lung, colorectal, and ovarian (PLCO) Cancer Screening...
Source: European Journal of Epidemiology - March 30, 2024 Category: Epidemiology Source Type: research
