A Facile Access to Green Fluorescent Albumin Derivatives
Chembiochem. 2024 Feb 18:e202300862. doi: 10.1002/cbic.202300862. Online ahead of print.ABSTRACTA Morita-Baylis-Hillman Adduct (MBHA) derivative bearing a triphenylamine moiety was found to react with human serum albumin (HSA) shifting its emission from the blue to the green-yellow thus leading to green fluorescent albumin (GFA) derivatives and enlarging the platform of probes for aggregation-induced fluorescent-based detection techniques. A possible interaction of MBHA derivative 7 with a lipophilic pocket within the HSA structure was suggested by docking studies. DLS experiments showed that the reaction with HSA induce a...
Source: Chembiochem - February 18, 2024 Category: Biochemistry Authors: Mario Saletti Marco Paolino Jacopo Venditti Claudia Bonechi Germano Giuliani Stefania Lamponi Giusy Tassone Antonella Boccia Chiara Botta Llu ís Blancafort Federica Poggialini Chiara Vagaggini Andrea Cappelli Source Type: research
Polysaccharide-rich extract of Genipa americana leaves protects seizures and oxidative stress in the mice model of pentylenetetrazole-induced epilepsy
This study aimed to evaluate the polysaccharide-rich extract of Genipa americana leaves (PRE-Ga) in the models of acute seizures and chronic epilepsy (kindling) induced by PTZ. In the acute seizure model, male Swiss mice (25-35 g) received PRE-Ga (1 or 9 mg/kg; intraperitoneal- IP), alone or associated with diazepam (0.01 mg/kg), 30 min before induction of seizures with PTZ (70 mg/kg; IP). In the chronic epilepsy model, seizures were induced by PTZ (40 mg/kg) 30 min after treatment and in alternated days up to 30 days and evaluated by video. Brain areas (prefrontal cortex, hippocampus, striatum) were assessed for inflammat...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - February 16, 2024 Category: Drugs & Pharmacology Authors: Dayanne Terra Ten ório Nonato Gislei Frota Arag ão Raquel Magalh ães Castelo Branco Craveiro Maria Gon çalves Pereira Silv ânia Maria Mendes Vasconcelos Deysi Viviana Tenazoa Wong Roberto C ésar Pereira Lima Júnior Pedro Marcos Gomes Soares Marcos Source Type: research
Benzodiazepines for the Treatment of Seizure Clusters
AbstractPatients with epilepsy may experience seizure clusters, which are described by the US Food and Drug Administration (FDA) as intermittent, stereotypic episodes of frequent seizure activity that are distinct from a patient ’s usual seizure pattern. Untreated seizure clusters may increase the risk for status epilepticus, as well as decrease quality of life and increase burden on patients and care partners. Benzodiazepine therapies are the mainstay for acute treatment of seizure clusters and are often administered by nonmedical care partners outside a healthcare facility. Three rescue therapies are currently FDA-appr...
Source: CNS Drugs - February 15, 2024 Category: Neurology Source Type: research
Molecular docking and GC/MS-based approach for identification of anxiolytic alkaloids from Griffinia (Amaryllidaceae) species in a zebrafish model
Chem Biodivers. 2024 Feb 14:e202302122. doi: 10.1002/cbdv.202302122. Online ahead of print.ABSTRACTGriffinia gardneriana Ravenna, Griffinia liboniana Morren and Griffinia nocturna Ravenna (Amarillydaceae) are bulbous plants found in tropical regions of Brazil. Our work aimed to determine the alkaloid profiles of Griffinia spp. and evaluate their anxiolytic potential through in vivo and in silico assays. The plants grown in greenhouses were dried and their ground bulbs were subjected to liquid-liquid partitions, resulting in alkaloid fractions that were analyzed by gas chromatography coupled to mass spectrometry (GC-MS). An...
Source: Chemistry and Biodiversity - February 14, 2024 Category: Biochemistry Authors: Elder Luis Lima Leite Ana Sheila Queiroz Souza Paulo Riceli Vasconcelos Ribeiro Rita de C ássia Alves Pereira Nat ália Florencio Martins Maria Kueirislene Amancio Ferreira Jane Eire Silva Alencar Menezes Helcio Silva Santos Otilia Deusdenia Loiola Pesso Source Type: research
Physician Approaches to the Pharmacologic Treatment of Dystonia in Cerebral Palsy
CONCLUSIONS: This study summarizes current indications and dosing for the six most commonly used medications to treat dystonia in CP as per treating physicians in the US and Canada and also demonstrates physician support for a randomized trial comparing the effectiveness of these treatments.ARTICLE SUMMARY: Dystonia is common and debilitating in people with CP, with little data on pharmacologic treatments. We describe physicians' current approaches to using these treatments.WHAT’S KNOWN ON THIS SUBJECT: Comparing the effectiveness of existing pharmacologic treatments for dystonia in CP is a research priority shared by cl...
Source: Pain Physician - February 14, 2024 Category: Anesthesiology Authors: Emma Lott Darcy Fehlings Rose Gelineau-Morel Michael Kruer Jonathan W Mink Sruthi P Thomas Steve Wisniewski Bhooma Aravamuthan Source Type: research
US Generic Antiseizure Medication Supply Chain: Observations from Analysis of US Government Databases
In 2015, the Center for Disease Control (CDC) estimated that in the U.S., 1.2% of the population have active epilepsy (including 3 million adults and approximately 500,000 children).[1] Over 25 antiseizure medications (ASM) are currently approved by the United States Food and Drug Administration (FDA).[2] During a critical shortage of an ASM, the feasibility of a rapid transition to an alternate ASM is often difficult.[3] The FDA drug shortage database in July 2023 indicated a national shortage of ASMs often used in status epilepticus, including rectal diazepam, oral clonazepam, and intravenous preparations of lorazepam, m...
Source: Seizure: European Journal of Epilepsy - February 4, 2024 Category: Neurology Authors: Pradeep Javarayee, Jennifer Meylor, Shamshad Sharukh, Shannon Pollock, Rene Andrade-Machado, Jeetendra Sah, Hema Patel Source Type: research
Successful treatment of severe alcohol withdrawal delirium with very high-dose diazepam (260-480 mg) administration
CONCLUSION: Severe AWS can potentially result in death otherwise managed quickly and adequately. Diazepam is a suitable agent for severe AWS or DT treatment. Clinicians should keep in mind that high-dose diazepam treatment may be required in the treatment of DT that develops after a long-term and high amount of alcohol consumption. Publications reporting the need for very high doses of diazepam in DT are limited and usually published long ago; in this context, our findings are significant. The evidence is often based on case reports and uncontrolled studies, so controlled trials are needed to determine optimal treatment do...
Source: Current Medical Research and Opinion - February 1, 2024 Category: Research Authors: Şükrü Alperen Korkmaz Ebru Aldemir Demet G üleç Öyekçin Source Type: research
Evaluation of spontaneous exploratory and anxiety-related behaviors of mice treated with gymnopilins obtained from the mushroom Gymnopilus imperialis (Agaricomycetes, Basidiomycota)
Toxicon. 2024 Jan 28;240:107626. doi: 10.1016/j.toxicon.2024.107626. Online ahead of print.ABSTRACTGymnopilins are long chain oligoisoprenoids produced through the condensation of isoprene units from MEV and MEP biosynthetic pathways. In Gymnopilus, these carotenoid-like molecules are recognized as major compounds in some species. In the present study, oligoisoprenoids derived from gymnopilins were dereplicated from Gymnopilus imperialis, a mushroom-forming basidiomycete, using liquid chromatographic coupled with high-resolution mass spectrometry (tandem LC-HRMS/MS) and GNPS. From the dichloromethane extract (Gym-DCM) of G...
Source: Toxicon - January 30, 2024 Category: Toxicology Authors: Lha ís Araújo Caldas Beatriz Gangale Muratori Andressa Gabriela Soliani Suzete Maria Cerutti Patr ícia Sartorelli Source Type: research
Prices, availability and affordability of selected essential medicines for chronic diseases in T ürkiye
CONCLUSION: This research highlights priority areas of action to improve access to affordable medicines for chronic diseases in Ankara, Türkiye.PMID:38279880 | DOI:10.26719/emhj.23.117 (Source: Eastern Mediterranean Health Journal)
Source: Eastern Mediterranean Health Journal - January 27, 2024 Category: Middle East Health Authors: G ökçen Özler O ğuz Işik Source Type: research
