Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Early Life Nutrition and the Developing Brain
J Fam Pract. 2023 Jul;72(6 Suppl):S25-S30. doi: 10.12788/jfp.0619.ABSTRACTThe developmental origins of health and disease (DOHaD) hypothesis suggests prenatal nutrition sets the stage for the developing brain, with effects that last into adulthood. Macronutrient and micronutrient requirements increase in pregnancy and deficiencies can influence fetal neurodevelopment and cognition. Foods such as eggs, meat, and seafood contain many of the nutrients needed for healthy neurodevelopment and intake should be encouraged among women of reproductive age. Family practice clinicians play an important role in providing nutrition rec...
Source: The Journal of Family Practice - August 7, 2023 Category: Practice Management Authors: Danielle Christifano Lara Bennett Source Type: research
Oriented-aptamer encoded magnetic nanosensor with laser-induced fluorescence for ultrasensitive test of okadaic acid
Talanta. 2023 Jul 27;266(Pt 1):124984. doi: 10.1016/j.talanta.2023.124984. Online ahead of print.ABSTRACTOkadaic acid (OA) biotoxin acts a well-established inhibitor of protein phosphatase even a tumor promoter of human being, arouse great attention in safety monitoring. However, the powerful and convenient nanosensing technologies for addressing the demands such as rapidity, high sensitivity, and stability in the in-field test of OA shellfish toxin is still scarce. Herein, a high-performance magnetic biometric nanosensor (MBNS) integrating oriented aptamers and ultrasensitive laser-induced fluorescence (LIF) was firstly p...
Source: Talanta - August 7, 2023 Category: Chemistry Authors: Yuwei Zhou Qingai Chen Guobin Huang Shukun Huang Chenchen Lin Xucong Lin Zenghong Xie Source Type: research
Early Life Nutrition and the Developing Brain
J Fam Pract. 2023 Jul;72(6 Suppl):S25-S30. doi: 10.12788/jfp.0619.ABSTRACTThe developmental origins of health and disease (DOHaD) hypothesis suggests prenatal nutrition sets the stage for the developing brain, with effects that last into adulthood. Macronutrient and micronutrient requirements increase in pregnancy and deficiencies can influence fetal neurodevelopment and cognition. Foods such as eggs, meat, and seafood contain many of the nutrients needed for healthy neurodevelopment and intake should be encouraged among women of reproductive age. Family practice clinicians play an important role in providing nutrition rec...
Source: The Journal of Family Practice - August 7, 2023 Category: Practice Management Authors: Danielle Christifano Lara Bennett Source Type: research
Oriented-aptamer encoded magnetic nanosensor with laser-induced fluorescence for ultrasensitive test of okadaic acid
Talanta. 2023 Jul 27;266(Pt 1):124984. doi: 10.1016/j.talanta.2023.124984. Online ahead of print.ABSTRACTOkadaic acid (OA) biotoxin acts a well-established inhibitor of protein phosphatase even a tumor promoter of human being, arouse great attention in safety monitoring. However, the powerful and convenient nanosensing technologies for addressing the demands such as rapidity, high sensitivity, and stability in the in-field test of OA shellfish toxin is still scarce. Herein, a high-performance magnetic biometric nanosensor (MBNS) integrating oriented aptamers and ultrasensitive laser-induced fluorescence (LIF) was firstly p...
Source: Talanta - August 7, 2023 Category: Chemistry Authors: Yuwei Zhou Qingai Chen Guobin Huang Shukun Huang Chenchen Lin Xucong Lin Zenghong Xie Source Type: research
Early Life Nutrition and the Developing Brain
J Fam Pract. 2023 Jul;72(6 Suppl):S25-S30. doi: 10.12788/jfp.0619.ABSTRACTThe developmental origins of health and disease (DOHaD) hypothesis suggests prenatal nutrition sets the stage for the developing brain, with effects that last into adulthood. Macronutrient and micronutrient requirements increase in pregnancy and deficiencies can influence fetal neurodevelopment and cognition. Foods such as eggs, meat, and seafood contain many of the nutrients needed for healthy neurodevelopment and intake should be encouraged among women of reproductive age. Family practice clinicians play an important role in providing nutrition rec...
Source: The Journal of Family Practice - August 7, 2023 Category: Practice Management Authors: Danielle Christifano Lara Bennett Source Type: research
Early Life Nutrition and the Developing Brain
J Fam Pract. 2023 Jul;72(6 Suppl):S25-S30. doi: 10.12788/jfp.0619.ABSTRACTThe developmental origins of health and disease (DOHaD) hypothesis suggests prenatal nutrition sets the stage for the developing brain, with effects that last into adulthood. Macronutrient and micronutrient requirements increase in pregnancy and deficiencies can influence fetal neurodevelopment and cognition. Foods such as eggs, meat, and seafood contain many of the nutrients needed for healthy neurodevelopment and intake should be encouraged among women of reproductive age. Family practice clinicians play an important role in providing nutrition rec...
Source: The Journal of Family Practice - August 7, 2023 Category: Practice Management Authors: Danielle Christifano Lara Bennett Source Type: research
Early Life Nutrition and the Developing Brain
J Fam Pract. 2023 Jul;72(6 Suppl):S25-S30. doi: 10.12788/jfp.0619.ABSTRACTThe developmental origins of health and disease (DOHaD) hypothesis suggests prenatal nutrition sets the stage for the developing brain, with effects that last into adulthood. Macronutrient and micronutrient requirements increase in pregnancy and deficiencies can influence fetal neurodevelopment and cognition. Foods such as eggs, meat, and seafood contain many of the nutrients needed for healthy neurodevelopment and intake should be encouraged among women of reproductive age. Family practice clinicians play an important role in providing nutrition rec...
Source: The Journal of Family Practice - August 7, 2023 Category: Practice Management Authors: Danielle Christifano Lara Bennett Source Type: research
Early Life Nutrition and the Developing Brain
J Fam Pract. 2023 Jul;72(6 Suppl):S25-S30. doi: 10.12788/jfp.0619.ABSTRACTThe developmental origins of health and disease (DOHaD) hypothesis suggests prenatal nutrition sets the stage for the developing brain, with effects that last into adulthood. Macronutrient and micronutrient requirements increase in pregnancy and deficiencies can influence fetal neurodevelopment and cognition. Foods such as eggs, meat, and seafood contain many of the nutrients needed for healthy neurodevelopment and intake should be encouraged among women of reproductive age. Family practice clinicians play an important role in providing nutrition rec...
Source: The Journal of Family Practice - August 7, 2023 Category: Practice Management Authors: Danielle Christifano Lara Bennett Source Type: research
