Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
Rapid visual detection of < em > Vibrio parahaemolyticus < /em > by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.PMID:37551560 | PMC:PMC10423967 | DOI:10.1631/jzus.B2200705 (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 8, 2023 Category: Science Authors: Fang Wu Chen Lu Wenhao Hu Xin Guo Jiayue Chen Zhidan Luo Source Type: research
