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Total 882 results found since Jan 2013.

Low dose of extracellular vesicles identified that promote recovery after ischemic stroke
Mesenchymal stem cell-derived extracellular vesicles (EVs) are one of the most promising therapeutics in protective and/or regenerative therapy in animal models of stroke using a dose of 100  μg. However, wheth...
Source: Stem Cell Research and Therapy - February 19, 2020 Category: Stem Cells Authors: Laura Otero-Ortega, Fernando Laso-Garc ía, Mari Carmen Gómez-de Frutos, Luke Diekhorst, Arturo Martínez-Arroyo, Elisa Alonso-López, María Laura García-Bermejo, Macarena Rodríguez-Serrano, Mercedes Arrúe-Gonzalo, Exuperio Díez-Tejedor, Blanca Fuen Tags: Research Source Type: research

Dose-dependent effects of tetramethylpyrazine on the characteristics of human umbilical cord mesenchymal stem cells for stroke therapy
This study determined the dose-dependent effects of tetramethylpyrazine (TMP) on the characteristics of ucMSCs in vitro. The effect on proliferation was determined with Cell Counting kit-8 assays. Cell migration was analyzed with Transwell assays and western blot analysis. Differentiation of ucMSCs was evaluated according to markers and the expression of relevant proteins and genes. Secretion capacity was detected by ELISA analysis. TMP protected ucMSCs against H2O2 induced-oxidative damage but had no influence on ucMSC activity at a low concentration. Furthermore, ucMSC migration was improved by TMP via the SDF-1/CXCR4 ax...
Source: Neuroscience Letters - February 15, 2020 Category: Neuroscience Source Type: research

Phytocannabinoids promote viability and functional adipogenesis of bone marrow-derived mesenchymal stem cells through different molecular targets.
In this study, we explored the potential therapeutic effect of some of the most abundant non-euphoric compounds derived from the Cannabis sativa plant (or phytocannabinoids) including tetrahydrocannabivarin (THCV), cannabidiol (CBD), cannabigerol (CBG), cannabidiolic acid (CBDA) and cannabigerolic acid (CBGA), by analysing their pharmacological activity on the viability of endogenous BM-MSCs as well as their ability to alter BM-MSC proliferation and differentiation into mature adipocytes. We provide evidence that CBD, CBDA, CBGA and THCV (5 µM) increase the number of viable BM-MSCs; whereas only CBG (5 µM) and CBD (5 µM...
Source: Biochemical Pharmacology - February 13, 2020 Category: Drugs & Pharmacology Authors: Fellous T, Di Maio F, Kalkann H, Carannante B, Boccella S, Petrosino S, Maione S, Di Marzo V, Arturo Iannotti F Tags: Biochem Pharmacol Source Type: research

Sanhua Decoction, a Classic Herbal Prescription, Exerts Neuroprotection Through Regulating Phosphorylated Tau Level and Promoting Adult Endogenous Neurogenesis After Cerebral Ischemia/Reperfusion Injury
Conclusions: SHD exerts neuroprotection probably by regulating p-tau level and promoting the proliferation, migration, and differentiation of endogenous neural stem cells, accompanying with neurobehavioral recovery.
Source: Frontiers in Physiology - February 11, 2020 Category: Physiology Source Type: research

Correction to: Intra-Arterial Transplantation of Low-Dose Stem Cells Provides Functional Recovery Without Adverse Effects After Stroke.
Abstract The original version of this article unfortunately contained an error in affiliation of Yuhtaka Fukuda. PMID: 32002776 [PubMed - as supplied by publisher]
Source: Cellular and Molecular Neurobiology - January 30, 2020 Category: Cytology Authors: Fukuda Y, Horie N, Satoh K, Yamaguchi S, Morofuji Y, Hiu T, Izumo T, Hayashi K, Nishida N, Nagata I Tags: Cell Mol Neurobiol Source Type: research

2020 Epilepsy Benchmarks Area III: Improved Treatment Options for Controlling Seizures and Epilepsy-Related Conditions Without Side Effects.
Authors: Traynelis SF, Dlugos D, Henshall D, Mefford HC, Rogawski MA, Staley KJ, Dacks PA, Whittemore V, Poduri A, National Institutes of Neurological Disorders and Stroke (NINDS)/American Epilepsy Society (AES) Epilepsy Research Benchmark Stewards Abstract The goals of Epilepsy Benchmark Area III involve identifying areas that are ripe for progress in terms of controlling seizures and patient symptoms in light of the most recent advances in both basic and clinical research. These goals were developed with an emphasis on potential new therapeutic strategies that will reduce seizure burden and improve quality of lif...
Source: Epilepsy Currents - January 23, 2020 Category: Neurology Tags: Epilepsy Curr Source Type: research

The Effect of Exosomes Derived from Bone Marrow Stem Cells in Combination with Rosuvastatin on Functional Recovery and Neuroprotection in Rats After Ischemic Stroke
In this study, neuroprotection and functional recovery of exosomes in combination with rosuvastatin have been investigated. Sixty adult male Wistar rats were subjected to middle cerebral artery occlusion (MCAO). Exosome at the dose of 100  μg and/or rosuvastatin at the dose of 20 mg/kg/day for 7 days were administered to rats as a therapeutic strategy. The elevated body swing test (EBST) and Garcia score were conducted as behavioral tests for the measurement of functional recovery. The histopathological and immunohistochemical an alyses were also performed for the assessment of infarcted volume and neuroprotection in t...
Source: Journal of Molecular Neuroscience - January 23, 2020 Category: Neuroscience Source Type: research

Early Transplantation of Human Cranial Bone-derived Mesenchymal Stem Cells Enhances Functional Recovery in Ischemic Stroke Model Rats.
Authors: Oshita J, Okazaki T, Mitsuhara T, Imura T, Nakagawa K, Otsuka T, Kurose T, Tamura T, Abiko M, Takeda M, Kawahara Y, Yuge L, Kurisu K Abstract We analyzed the cell characteristics, neuroprotective, and transplantation effects of human cranial bone-derived mesenchymal stem cells (hcMSCs) in ischemic stroke model rats compared with human iliac bone-derived mesenchymal stem cells (hiMSCs). The expressions of brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF ) as neurotrophic factors were analyzed in both MSCs. hiMSCs or hcMSCs were intravenously administered into ischemic st...
Source: Neurologia Medico-Chirurgica - January 22, 2020 Category: Neurosurgery Tags: Neurol Med Chir (Tokyo) Source Type: research

Combination of Human Mesenchymal Stem Cells and Repetitive Transcranial Magnetic Stimulation Enhances Neurological Recovery of 6-Hydroxydopamine Model of Parkinsonian ’s Disease
Conclusion:Our findings demonstrate that rTMS treatment and hMSC transplantation could synergistically create a favorable microenvironment for cell survival within the PD rat brain, through alteration of soluble factors such as neurotrophic/growth factors and anti-/pro-inflammatory cytokines related to neuronal protection or repair, with preservation of DA neurons and improvement of motor functions.
Source: Tissue Engineering and Regenerative Medicine - January 22, 2020 Category: Biotechnology Source Type: research

Systemic conditioned medium treatment from interleukin-1 primed mesenchymal stem cells promotes recovery after stroke
Mesenchymal stem cells (MSCs) hold great potential as a therapy for stroke and have previously been shown to promote recovery in preclinical models of cerebral ischaemia. MSCs secrete a wide range of growth fa...
Source: Stem Cell Research and Therapy - January 21, 2020 Category: Stem Cells Authors: Catriona J. Cunningham, Raymond Wong, Jack Barrington, Sabrina Tamburrano, Emmanuel Pinteaux and Stuart M. Allan Tags: Research Source Type: research

Immunotherapy for Parkinson's disease.
Abstract With the increasing prevalence of Parkinson's disease, there is an immediate need to interdict disease signs and symptoms. In recent years this need was met through therapeutic approaches focused on regenerative stem cell replacement and alpha-synuclein clearance. However, neither have shown long-term clinical benefit. A novel therapeutic approach designed to affect disease is focused on transforming the brain's immune microenvironment. As disordered innate and adaptive immune functions are primary components of neurodegenerative disease pathogenesis, this has emerged as a clear opportunity for therapeuti...
Source: Neurobiology of Disease - January 21, 2020 Category: Neurology Authors: Schwab AD, Thurston MJ, Machhi JP, Olson KE, Namminga KL, Gendelman HE, Mosley RL Tags: Neurobiol Dis Source Type: research

Fetal Bovine Serum-More Data
Primary and Stem Cell CultureThis just came across our radar." SUPPLEMENTARY MATERIAL An eye opener in stroke: Mitochondrial dysfunction and stem cell repair in MCAO induced retinal ischemia "We are always delighted when researchers supplement their cell culture media with ourFetal Bovine Serum (FBS).RPE Cells and MSC Culture Retinal pigmented epithelium (RPE, CRL-4000; ATCC) cells were cultured in Dulbecco ’s Modified Eagle Media/F-12 (DMEM/F-12, 11320033; Gibco) containing 10% fetal bovine serum (FBS; FBS001; Neuromics) and 0.01 mg/ml hygromycin B (10687010; Gibco) in incubator (37 °C humidified, with 5% CO2, 95% air)...
Source: Neuromics - January 17, 2020 Category: Neuroscience Tags: adult stem cells FBS Fetal Bovine Serum Human Mesenchymal Stem Cells MSCs Retinal Pigment Epithelial Cells RPES Source Type: news

Toxicity and health impact of nanoparticles. Basic biology and clinical perspective.
Authors: Olaru DG, Olaru A, Kassem GH, Popescu-Drigă MV, Pinoşanu LR, Dumitraşcu DI, Popescu EL, Hermann DM, Popa-Wagner A Abstract Stroke has limited restorative treatment options. In search of new therapeutic strategies for the ischemic brain, cell-based therapies offered new hope, which has been, in the meanwhile, converted into a more realistic approach recognizing difficulties related to unfavorable environments causing low survival rates of transplanted neuronal precursors. Stem cell therapies are based on the transplantation of neuronal precursor cells (NPCs), adult stem cells propagated in cell culture o...
Source: Romanian Journal of Morphology and Embryology - January 10, 2020 Category: General Medicine Tags: Rom J Morphol Embryol Source Type: research

Aspergillus fumigatus endocarditis in a splenectomized patient with no risk factors
Publication date: Available online 9 January 2020Source: IDCasesAuthor(s): Abhimanyu Aggarwal,, Karen Hogan,, Armando Paez,AbstractAspergillus endocarditis is a rare cause of culture-negative fungal endocarditis, after Candida endocarditis. Typical risk factors include intravenous drug use, immunosuppression, prior cardiac surgery or presence of prosthetic heart valves, hematopoietic stem cell or solid organ transplantation. Common presentations include signs and symptoms consistent with endocarditis but with negative bacterial blood cultures. Here, we present a case report of a 49-year-old male without known risk factors ...
Source: IDCases - January 9, 2020 Category: Infectious Diseases Source Type: research

Combining growth factor and stem cell therapy for stroke rehabilitation, a review.
Abstract Stroke is a serious, life-threatening condition demanding vigorous search for new therapies. Recent research has focused on stem cell-based therapies as a viable choice following ischemic stroke, based on studies displaying that stem cells transplanted to the brain not only survive, but also cause functional recovery. Growth factors defined as polypeptides that regulate the growth and differentiation of many cell types. Many studies have demonstrated that combination use of growth factors may increase results by stimulation of endogenous neurogenesis, anti-inflammatory, neuroprotection properties, and enh...
Source: Current Drug Targets - January 6, 2020 Category: Drugs & Pharmacology Authors: Asgharzade S, Talaei A, Farkhondeh T, Forouzanfar F Tags: Curr Drug Targets Source Type: research

Neural Stem Cells.
Authors: Tuazon JP, Castelli V, Lee JY, Desideri GB, Stuppia L, Cimini AM, Borlongan CV Abstract Neural stem cell (NSC) transplantation has provided the basis for the development of potentially powerful new therapeutic cell-based strategies for a broad spectrum of clinical diseases, including stroke, psychiatric illnesses such as fetal alcohol spectrum disorders, and cancer. Here, we discuss pertinent preclinical investigations involving NSCs, including how NSCs can ameliorate these diseases, the current barriers hindering NSC-based treatments, and future directions for NSC research. There are still many translatio...
Source: Advances in Experimental Medicine and Biology - January 5, 2020 Category: Research Tags: Adv Exp Med Biol Source Type: research

Reproducibility of GMP-compliant production of therapeutic stressed peripheral blood mononuclear cell-derived secretomes, a novel class of biological medicinal products.
CONCLUSIONS: We are the first to demonstrate the consistent production of cell-derived, yet cell-free secretome as a biological medicinal product. The results from this study provide the basis for selecting appropriate quality control parameters for GMP-compliant production of therapeutic cell secretomes and pave the way for future clinical trials employing secretomes in tissue regenerative medicine. PMID: 31900195 [PubMed - in process]
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Laggner M, Gugerell A, Bachmann C, Hofbauer H, Vorstandlechner V, Seibold M, Gouya Lechner G, Peterbauer A, Madlener S, Demyanets S, Sorgenfrey D, Ostler T, Erb M, Mildner M, Ankersmit HJ Tags: Stem Cell Res Ther Source Type: research

64Cu PET Imaging of the CXCR4 Chemokine Receptor Using a Cross-Bridged Cyclam Bis-Tetraazamacrocyclic Antagonist
Conclusion: The tetraazamacrocyclic small molecule 64Cu-CuCB-bicyclam has been shown to be an imaging agent for the CXCR4 receptor that is likely to be applicable across a range of species. It has high affinity and stability and is suitable for preclinical research in immunocompetent murine models.
Source: Journal of Nuclear Medicine - January 2, 2020 Category: Nuclear Medicine Authors: Burke, B. P., Miranda, C. S., Lee, R. E., Renard, I., Nigam, S., Clemente, G. S., DHuys, T., Ruest, T., Domarkas, J., Thompson, J. A., Hubin, T. J., Schols, D., Cawthorne, C. J., Archibald, S. J. Tags: Basic Source Type: research

Cuprizone-induced demyelination under physiological and post-stroke condition leads to decreased neurogenesis response in adult mouse brain.
Abstract Due to the limitation in treatment window of the rtPA (recombinant tissue plasminogen activator), the development of delayed treatment for stroke is needed. We previously reported that there is a difference in neurogenesis and neuroblast migration patterns in different mouse stroke models (proximal and distal middle cerebral artery occlusion models, pMCAo or dMCAo). Specifically, compared to robust neurogenesis and substantial migration of newly born neuroblasts in pMCAo model, dMCAo only illicit limited neurogenesis and migration of neuroblasts towards ischemic area. One potential reason for this differe...
Source: Experimental Neurology - January 2, 2020 Category: Neurology Authors: Luo F, Zhang Z, Barnett A, Bellinger TJ, Turcato F, Schmidt K, Luo Y Tags: Exp Neurol Source Type: research

GSE120701 Developmentally controlled expression of Cxcr4 by hematopoietic progenitors allows tracing of monocyte function in stroke
Contributors : Yves Werner ; Elvira MassSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusMonocyte-derived and tissue-resident macrophages, such as microglia, belong to independent lineages with distinct functions in steady state. During inflammation, however, changes in phenotypes and transcriptional profiles make it difficult to assess their respective roles in pathology. Here, we report that developmentally controlled expression of Cxcr4 by hematopoietic progenitors allows highly specific inducible lineage tracing of hematopoietic stem cell-derived monocytes during inflammation. In t...
Source: GEO: Gene Expression Omnibus - December 31, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

Cerebral Organoids Repair Ischemic Stroke Brain Injury
AbstractStroke is the second leading cause of death and main cause of disability worldwide, but with few effective therapies. Although stem cell-based therapy has been proposed as an exciting regenerative medicine strategy for brain injury, there are limitations. The developed cerebral organoids (COs) represent a promising transplantation source for stroke that remains to be answered. Here, we transplanted COs at 55  days and explored the feasibility in the rat middle cerebral artery occlusion (MCAO) model of stroke. COs transplantation at 6 h or even 24 h after MCAO significantly reduces brain infarct volume and improv...
Source: Translational Stroke Research - December 30, 2019 Category: Neurology Source Type: research

The Effects of Electroacupuncture in a Rat Model of Cerebral Ischemia-Reperfusion Injury Following Middle Cerebral Artery Occlusion Involves MicroRNA-223 and the PTEN Signaling Pathway.
CONCLUSIONS In a rat model of CIRI following MCAO, EA activated the NOTCH pathway, promoted the expression of miR-223, increased the number of NSCs, and reduced the expression of PTEN. PMID: 31883264 [PubMed - in process]
Source: Medical Science Monitor - December 29, 2019 Category: Research Tags: Med Sci Monit Source Type: research

Environmental Influences on Clonal Hematopoiesis
Clonal hematopoiesis (CH) is a condition in which one or a few individual hematopoietic stem cells (HSCs) contribute disproportionately to peripheral blood production [1 –3]. Not only are individuals with CH at significantly greater risk of developing hematologic malignancies compared to their non-CH counterparts, but importantly they have greater all-cause mortality largely from heart disease and stroke [2, 4]. The prevalence of clonal hematopoiesis increases wit h age and is thought to occur at some level in virtually all people [5], such that around 20% of individuals above the age of 70 have at least one HSC clone th...
Source: Experimental Hematology - December 29, 2019 Category: Hematology Authors: Katherine Y. King, Yun Huang, Daisuke Nakada, Margaret A. Goodell Source Type: research

A Comparison of Intracerebral Transplantation of RMNE6 Cells and MSCs on Ischemic Stroke Models
Conclusion: MSCs are still the preferred cells for cell replacement in stroke therapy, while RMNE6 cells need to be modified.
Source: Neurology India - December 20, 2019 Category: Neurology Authors: Yun Wu Li-Wang Yang Xiao-Yan Zhai Jian-Chun Liu Source Type: research

Stem Cell Therapy for Ischemic Stroke Injury: A Current Status
Pius PadayattiNeurology India 2019 67(6):1491-1491
Source: Neurology India - December 20, 2019 Category: Neurology Authors: Pius Padayatti Source Type: research

Therapeutic Effect of Autologous Bone Marrow Stem Cell Mobilization Combined with Anti-infective Therapy on Moyamoya Disease
Conclusion: autogenous bone marrow stem cell mobilization combined with dexamethasone anti-inflammation and anti-infection treatment after revascularization in patients with MMD can accelerate the recovery of nerve function and promote the formation of new blood vessels. At the same time, it can reduce inflammation and improve patients' quality of life, which is worthy of clinical reference.
Source: Saudi Journal of Biological Sciences - December 19, 2019 Category: Biology Source Type: research

Transplantation of Lymphocytes Co-Cultured with Human Cord Blood-Derived Multipotent Stem Cells Attenuates Inflammasome Activity in Ischemic Stroke
Source: Clinical Interventions in Aging - December 18, 2019 Category: Geriatrics Tags: Clinical Interventions in Aging Source Type: research

Neuroprotective effects of human neural stem cells over-expressing choline acetyltransferase in a middle cerebral artery occlusion model
Publication date: January 2020Source: Journal of Chemical Neuroanatomy, Volume 103Author(s): Jihyun Kim, Kyungha Shin, Yeseul Cha, Young-Hwan Ban, Sung Kyeong Park, Heon Sang Jeong, Dongsun Park, Ehn-Kyoung Choi, Yun-Bae KimAbstractStroke is one of the most-devastating brain diseases causing acute death or permanent disability. Although tissue-type plasminogen activator was approved by Food and Drug Administration for early reperfusion of the occluded vessels, oxidative injury may cause extensive brain infarction. Accordingly, there is a need for effective neuroprotection during reperfusion, and stem cell-based therapeutic...
Source: Journal of Chemical Neuroanatomy - December 17, 2019 Category: Neuroscience Source Type: research

Stroke drug boosts stem cell therapy for spinal cord injury in rats
(University of California - San Diego) In a UC San Diego study, rats with spinal cord injuries experienced a three-fold increase in motor activity when treated with neural progenitor cells that had been pre-conditioned with a modified form of tPA, a drug commonly used to treat non-hemorrhagic stroke.
Source: EurekAlert! - Medicine and Health - December 17, 2019 Category: International Medicine & Public Health Source Type: news

An update on clinical, pathological, diagnostic, and therapeutic perspectives of childhood leukodystrophies.
Authors: Ashrafi MR, Amanat M, Garshasbi M, Kameli R, Nilipour Y, Heidari M, Rezaei Z, Tavasoli AR Abstract Introduction: Leukodystrophies constitute heterogenous group of rare heritable disorders primarily affecting the white matter of central nervous system. These conditions are often under-appreciated among physicians. The first clinical manifestations of leukodystrophies are often nonspecific and can occur in different ages from neonatal to late adulthood periods. The diagnosis is, therefore, challenging in most cases.Area covered: Herein, the authors discuss different aspects of leukodystrophies. The authors used...
Source: Expert Review of Neurotherapeutics - December 13, 2019 Category: Neurology Tags: Expert Rev Neurother Source Type: research

Differential susceptibility of human neural progenitors and neurons to ischaemic injury.
CONCLUSIONS: Highly arbourised neurons are more sensitive to ischaemic injury than NPs which did however develop marked vulnerability to prolonged injury with reoxygenation. These observations imply that therapeutic potential may be highly dependent of the developmental state of the neurons we aim to protect. PMID: 31837459 [PubMed - as supplied by publisher]
Source: Brain Research Bulletin - December 11, 2019 Category: Neurology Authors: Liu Y, Michalska AE, Dottori M, Eaton E, Courtney JM, Antonic A, Howells DW Tags: Brain Res Bull Source Type: research

Intra-arterial Stem Cell Therapy Diminishes Inflammasome Activation After Ischemic Stroke: a Possible Role of Acid Sensing Ion Channel 1a
This study offers a preliminary evidence of the role of IA MSCs in regulating inflammasome by modulating ASIC1a.
Source: Journal of Molecular Neuroscience - December 9, 2019 Category: Neuroscience Source Type: research

Neural Stem Cell Extracellular Vesicles Disrupt Midline Shift Predictive Outcomes in Porcine Ischemic Stroke Model
This study investigated the utility of MRI as a predictive measure of clinical and functional outcomes when a stroke intervention is withheld or provided, in order to identify biomarkers for stroke functional outcome under these conditions. Fifteen MRI and ninety functional parameters were measured in a middle cerebral artery occlusion (MCAO) porcine ischemic stroke model. Multiparametric analysis of correlations between MRI measurements and functional outcome was conducted. Acute axial and coronal midline shift (MLS) at 24 h post-stroke were associated with decreased survival and recovery measured by modified Rankin scale...
Source: Translational Stroke Research - December 6, 2019 Category: Neurology Source Type: research

Long-term results of isolated transmyocardial laser revascularization in combination with the intramyocardial autologous bone marrow stem cells injection
AbstractTo evaluate the long-term results of TMLR using a CO2 laser in combination with intramyocardial injection of ABMSC as an isolated procedure in patients with the end-stage coronary artery disease, the study included 20 patients (90% male), with a mean age of 58.4 ± 8.7 years. To assess the long-term results, patients were examined in a hospital. The Minnesota Living with Heart Failure Questionnaire (MLHFQ) and the Seattle Angina Questionnaire (SAQ) were used. The evolution of laboratory and instrumental indices, as well as medical therapy, was assessed. The end points of the study were death, acute myocardial infar...
Source: Lasers in Medical Science - December 4, 2019 Category: Laser Surgery Source Type: research

From the Lab to Patients: a Systematic Review and Meta-Analysis of Mesenchymal Stem Cell Therapy for Stroke
AbstractThere may be the potential to improve stroke recovery with mesenchymal stem cells (MSCs); however, questions about the efficacy and safety of this treatment remain. To address these issues and inform future studies, we performed a preclinical and clinical systematic review of MSC therapy for subacute and chronic ischemic stroke. MEDLINE, Embase, the Cochrane Register of Controlled Trials, and PubMed were searched. For the clinical review, interventional and observational studies of MSC therapy in ischemic stroke patients were included. For the preclinical review, interventional studies of MSC therapy  usingin vivo...
Source: Translational Stroke Research - October 25, 2019 Category: Neurology Source Type: research

Adult Neurogenesis in the Subventricular Zone and Its Regulation After Ischemic Stroke: Implications for Therapeutic Approaches
AbstractAdult neurogenesis in the subventricular zone is a topic of intense research, since it has vast implications for the fundamental understanding of the neurobiology of the brain and its potential to being harnessed for therapy in various neurological disorders. Investigation of adult neurogenesis has been complicated by the difficulties with characterization of neural stem cells in vivo. However, recent single-cell transcriptomic studies provide more detailed information on marker expression in neural stem cells and their neuronal lineage, which hopefully will result in a more unified discussion. Regulation of the mu...
Source: Translational Stroke Research - July 15, 2019 Category: Neurology Source Type: research

Application of Mesenchymal Stem Cell-Derived Extracellular Vesicles for Stroke: Biodistribution and MicroRNA Study
In this study, we investigated the biodistribution, therapeutic efficacy, and mode of action of MSC-EVs in a rat stroke model. MSC-EVs successfully stimulated neurogenesis and angiogenesis in vivo. When compared to the MSC-treated group, rats treated with MSC-EVs exhibited greater behavioral improvements than the control group (p <  0.05). Our biodistribution study using fluorescence-labeled MSC-EVs and MSCs demonstrated that the amounts of MSC-EVs in the infarcted hemisphere increased in a dose-dependent manner, and were rarely found in the lung and liver. In addition, MSC-EVs were highly inclusive of various prote...
Source: Translational Stroke Research - October 19, 2018 Category: Neurology Source Type: research

Human Neural Stem Cell Extracellular Vesicles Improve Tissue and Functional Recovery in the Murine Thromboembolic Stroke Model
AbstractOver 700 drugs have failed in stroke clinical trials, an unprecedented rate thought to be attributed in part to limited and isolated testing often solely in “young” rodent models and focusing on a single secondary injury mechanism. Here, extracellular vesicles (EVs), nanometer-sized cell signaling particles, were tested in a mouse thromboembolic (TE) stroke model. Neural stem cell (NSC) and mesenchymal stem cell (MSC) EVs derived from the same pluri potent stem cell (PSC) line were evaluated for changes in infarct volume as well as sensorimotor function. NSC EVs improved cellular, tissue, and functional outcome...
Source: Translational Stroke Research - October 1, 2018 Category: Neurology Source Type: research

Hydrogel Scaffolds: Towards Restitution of Ischemic Stroke-Injured Brain
AbstractChronic brain injury following cerebral ischemia is a severe debilitating neurological condition, where clinical intervention is well known to decrease morbidity and mortality. Despite the development of several therapeutic strategies, clinical outcome in the majority of patients could be better improved, since many still face life-long neurological deficits. Among the several strategic options that are currently being pursued, tissue engineering provides much promise for neural tissue salvage and regeneration in brain ischemia. Specifically, hydrogel biomaterials have been utilized to docket biomolecules, adhesion...
Source: Translational Stroke Research - August 27, 2018 Category: Neurology Source Type: research

Role of Exosomes as a Treatment and Potential Biomarker for Stroke
AbstractApproximately, 16 million strokes occur worldwide each year, causing 6 million deaths and considerable disability, implying an enormous social, individual health, and economic burden. Due to this high incidence, strategies to promote stroke recovery are urgently needed. Research into new therapeutic approaches for stroke has determined that intravenous administration of mesenchymal stem cells (MSCs) is a good strategy to improve recovery by amplifying mechanisms implicated in brain plasticity. Recent studies have demonstrated the efficacy of MSCs in stroke, with no need for them to reach the area of brain injury. A...
Source: Translational Stroke Research - August 13, 2018 Category: Neurology Source Type: research

Mitochondrial Dysfunction in Stroke: Implications of Stem Cell Therapy
AbstractStroke is a debilitating condition which is also the second leading cause of death and disability worldwide. Despite the benefits and promises shown by numerous neuroprotective agents in animal stroke models, their clinical translation has not been a complete success. Hence, search for treatment options have directed researchers towards utilising stem cells. Mitochondria has a major involvement in the pathophysiology of stroke and a number of other conditions. Stem cells have shown the ability to transfer mitochondria to the damaged cells and to help revive cell energetics in the recipient cell. The present review ...
Source: Translational Stroke Research - June 20, 2018 Category: Neurology Source Type: research

Stem Cell-Based Immunomodulation After Stroke Topical Review
Source: Stroke - May 25, 2018 Category: Neurology Authors: Marieke C.S. Boshuizen, Gary K. Steinberg Tags: Inflammation, Stem Cells, Ischemic Stroke Topical Reviews Source Type: research

Human Neural Stem Cell Extracellular Vesicles Improve Recovery in a Porcine Model of Ischemic Stroke Basic Sciences
Conclusions—This study demonstrated for the first time that in a large animal model novel NSC EVs significantly improved neural tissue preservation and functional levels post-MCAO, suggesting NSC EVs may be a paradigm changing stroke therapeutic.
Source: Stroke - April 23, 2018 Category: Neurology Authors: Robin L. Webb, Erin E. Kaiser, Brian J. Jurgielewicz, Samantha Spellicy, Shelley L. Scoville, Tyler A. Thompson, Raymond L. Swetenburg, David C. Hess, Franklin D. West, Steven L. Stice Tags: Animal Models of Human Disease, Basic Science Research, Translational Studies, Treatment, Ischemic Stroke Original Contributions Source Type: research

Acute or Delayed Systemic Administration of Human Amnion Epithelial Cells Improves Outcomes in Experimental Stroke Basic Sciences
Conclusions—Systemic poststroke administration of hAECs elicits marked neuroprotection and facilitates mechanisms of repair and recovery.
Source: Stroke - February 26, 2018 Category: Neurology Authors: Megan A. Evans, Rebecca Lim, Hyun Ah Kim, Hannah X. Chu, Chantelle V. Gardiner-Mann, Kimberly W.E. Taylor, Christopher T. Chan, Vanessa H. Brait, Seyoung Lee, Quynh Nhu Dinh, Antony Vinh, Thanh G. Phan, Velandai K. Srikanth, Henry Ma, Thiruma V. Arumugam, Tags: Animal Models of Human Disease, Cell Therapy, Stem Cells, Cerebrovascular Disease/Stroke, Ischemic Stroke Original Contributions Source Type: research

Human Muse Cells Reconstruct Neuronal Circuitry in Subacute Lacunar Stroke Model Basic Sciences
Conclusions—Transplantation at the delayed subacute phase showed muse cells differentiated into neural cells, facilitated neural reconstruction, improved functions, and displayed solid safety outcomes over prolonged graft maturation period, indicating their therapeutic potential for lacunar stroke.
Source: Stroke - January 23, 2017 Category: Neurology Authors: Hiroki Uchida, Kuniyasu Niizuma, Yoshihiro Kushida, Shohei Wakao, Teiji Tominaga, Cesario V. Borlongan, Mari Dezawa Tags: Cell Therapy, Stem Cells, Translational Studies, Ischemic Stroke Original Contributions Source Type: research

Neurorestorative Responses to Delayed Human Mesenchymal Stromal Cells Treatment of Stroke in Type 2 Diabetic Rats Basic Sciences
Conclusions—In T2DM stroke rats, delayed hMSC treatment significantly improves neurological functional outcome and increases neurorestorative effects and M2 macrophage polarization. Increasing brain platelet-derived growth factor expression may contribute to hMSC-induced neurorestoration.
Source: Stroke - October 24, 2016 Category: Neurology Authors: Yan, T., Venkat, P., Chopp, M., Zacharek, A., Ning, R., Roberts, C., Zhang, Y., Lu, M., Chen, J. Tags: Cell Therapy, Stem Cells, Diabetes, Type 2, Cerebrovascular Disease/Stroke, Ischemic Stroke Original Contributions Source Type: research

Systematic Review and Meta-Analysis of Bone Marrow-Derived Mononuclear Cells in Animal Models of Ischemic Stroke Basic Sciences
Conclusions— BMMNCs demonstrate beneficial effects across histological and behavioral outcomes in animal ischemic stroke models. Although study quality has improved over time, considerable degree of heterogeneity calls for standardization in the conduct and reporting of experimentation.
Source: Stroke - May 23, 2016 Category: Neurology Authors: Vahidy, F. S., Rahbar, M. H., Zhu, H., Rowan, P. J., Bambhroliya, A. B., Savitz, S. I. Tags: Cell Therapy, Stem Cells, Ischemic Stroke Basic Sciences Source Type: research

Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke Basic Sciences
Conclusions— hBMSC transplantation is therapeutic in chronic stroke possibly by abrogating the inflammation-plagued secondary cell death.
Source: Stroke - August 24, 2015 Category: Neurology Authors: Acosta, S. A., Tajiri, N., Hoover, J., Kaneko, Y., Borlongan, C. V. Tags: Animal models of human disease, Pathology of Stroke, Other Stroke Treatment - Surgical Basic Sciences Source Type: research

Delayed Pituitary Adenylate Cyclase-Activating Polypeptide Delivery After Brain Stroke Improves Functional Recovery by Inducing M2 Microglia/Macrophage Polarization Basic Sciences
Conclusions— Our results demonstrated that immunomodulatory strategies capable of redirecting the microglial response toward a neuroprotective M2 phenotype in the late phase of brain ischemia could represent attractive options for stroke treatment in a new and unexploited therapeutical window.
Source: Stroke - January 26, 2015 Category: Neurology Authors: Brifault, C., Gras, M., Liot, D., May, V., Vaudry, D., Wurtz, O. Tags: Embolic stroke Basic Sciences Source Type: research

Intravenous Autologous Bone Marrow Mononuclear Stem Cell Therapy for Ischemic Stroke: A Multicentric, Randomized Trial Clinical Sciences
Conclusions— With the methods used, results of this hitherto first randomized controlled trial indicate that intravenous infusion of BMSCs is safe, but there is no beneficial effect of treatment on stroke outcome. Clinical Trial Registration— URLs: http://ctri.nic.in/Clinicaltrials and http://www.clinicaltrials.gov. Unique identifiers: CTRI-ROVCTRI/2008/091/0004 and NCT0150177.
Source: Stroke - November 24, 2014 Category: Neurology Authors: Prasad, K., Sharma, A., Garg, A., Mohanty, S., Bhatnagar, S., Johri, S., Singh, K. K., Nair, V., Sarkar, R. S., Gorthi, S. P., Hassan, K. M., Prabhakar, S., Marwaha, N., Khandelwal, N., Misra, U. K., Kalita, J., Nityanand, S., for InveST Study Group Tags: Other Stroke Treatment - Medical Clinical Sciences Source Type: research