Query: stroke

Filtered By:
Nutrition: Chloride

This page shows you your search results in order of date.

Order by Relevance | Date

Total 200 results found since Jan 2013.

Loop Diuretics Inhibit Ischemia-Induced Intracellular Ca2+ Overload in Neurons via the Inhibition of Voltage-Gated Ca2+ and Na+ Channels
One consequence of ischemic stroke is disruption of intracellular ionic homeostasis. Intracellular overload of both Na+ and Ca2+ has been linked to neuronal death in this pathophysiological state. The etiology of ionic imbalances resulting from stroke-induced ischemia and acidosis includes the dysregulation of multiple plasma membrane transport proteins, such as increased activity of sodium-potassium-chloride cotransporter-1 (NKCC-1). Experiments using NKCC1 antagonists, bumetanide (BMN) and ethacrynic acid (EA), were carried out to determine if inhibition of this cotransporter affects Na+ and Ca2+ overload observed follow...
Source: Frontiers in Pharmacology - September 15, 2021 Category: Drugs & Pharmacology Source Type: research

Salt substitution reduces the rate of cardiovascular events and death
Nature Reviews Cardiology, Published online: 13 September 2021; doi:10.1038/s41569-021-00615-3In the SSaSS trial, substituting regular table salt (100% sodium chloride) with an alternative containing 25% potassium chloride reduced the rate of stroke, major cardiovascular events and all-cause death.
Source: Nature Reviews Cardiology - September 13, 2021 Category: Cardiology Authors: Gregory B. Lim Source Type: research

Behavioral and histopathological consequences of transient ischemic stroke in the Flinders Sensitive Line rat, a genetic animal model of depression
Brain Res. 2021 Sep 4:147648. doi: 10.1016/j.brainres.2021.147648. Online ahead of print.ABSTRACTPatients with depression have an increased risk for stroke, higher mortality rates following stroke and worse functional outcomes among survivors. Preclinical studies may help to better understand the underlying mechanisms linking these two diseases, but only a few animal studies have investigated the effects of prestroke depression. The present study investigates whether Flinders Sensitive Line (FSL) rats, a genetic depression model, respond differently to focal ischemic stroke compared to control strains (Flinders Resistant L...
Source: Brain Research - September 7, 2021 Category: Neurology Authors: Denise F Happ Gregers Wegener R Andrew Tasker Source Type: research

Protective effects of Dimethyl malonate on neuroinflammation and blood-brain barrier after ischemic stroke
Conclusions DMM could regulate activation and polarization of microglia to inhibit neuroinflammation and protect BBB.
Source: NeuroReport - September 3, 2021 Category: Neurology Tags: Degeneration and Repair Source Type: research

Decreased spasticity of Baishaoluoshi Decoction through the BDNF/TrKB-KCC2 pathway on poststroke spasticity rats
Conclusion Suppression of spasticity by Baishaoluoshi Decoction in PSS rat models may be correlated with upregulated BDNF/TrKB-KCC2 pathway, which may be a complementary therapeutic strategy for PSS.
Source: NeuroReport - September 3, 2021 Category: Neurology Tags: Cellular, Molecular and Developmental Neuroscience Source Type: research

Guhong Injection Alleviates Cerebral Ischemia –Reperfusion Injury via the PKC/HIF-1α Pathway in Rats
In this study, we investigated the curative effect of GHI on cerebral ischemia–reperfusion (I/R) injury via the PKC/HIF-1α pathway in rats. Adult male Sprague Dawley rats were randomly divided into seven groups: sham-operated, middle cerebral artery occlusion (MCAO), GHI, nimodipine injection (NMDP), MCAO + LY317615 (PKC inhibitor), GHI + LY317615, and NMDP + LY317615. After establishing an MCAO rat model, we performed neurological deficit testing, 2,3,5-triphenyltetrazolium chloride staining, hematoxylin and eosin (HE) staining, enzyme-linked immunosorbent assay, Western blotting, and q-PCR to detect the brain damage i...
Source: Frontiers in Pharmacology - September 2, 2021 Category: Drugs & Pharmacology Source Type: research

Electroacupuncture Regulates Endoplasmic Reticulum Stress and Ameliorates Neuronal Injury in Rats with Acute Ischemic Stroke
This study aimed to determine whether endoplasmic reticulum stress (ERS) could contribute to the protective effects of EA in cerebral ischemia/reperfusion injury (CIRI) to provide a rationale for the widespread clinical use of EA. Rats were divided into the sham-operated (sham) group, the CIRI (model) group, and the EA group. Rats in the model group were subjected to middle cerebral artery occlusion (MCAO) for 2 h followed by 72 h of reperfusion. Rats with CIRI were treated daily with EA at GV20 and ST36 for a total of 3 days. The Longa scoring system and adhesive removal somatosensory test were applied to evaluate neurolo...
Source: Evidence-based Complementary and Alternative Medicine - August 26, 2021 Category: Complementary Medicine Authors: Ya-Min Zhang Hong Xu Su-Hui Chen Hua Sun Source Type: research

Aloperine protects against cerebral ischemia/reperfusion injury via activating the PI3K/AKT signaling pathway in rats
Exp Ther Med. 2021 Oct;22(4):1045. doi: 10.3892/etm.2021.10478. Epub 2021 Jul 22.ABSTRACTCerebral ischemia is among the leading causes of death and long-term disability worldwide. The aim of the present study was to investigate the effects of aloperine (ALO) on cerebral ischemia/reperfusion (I/R) injury in rats and elucidate the possible underlying mechanisms. Therefore, a rat model of reversible middle cerebral artery occlusion (MCAO) was established to induce cerebral I/R injury. Following pretreatment with different doses of ALO, the histopathological changes in the brain tissue were evaluated by hematoxylin and eosin s...
Source: Experimental and Therapeutic Medicine - August 26, 2021 Category: General Medicine Authors: Zhimin Li Xing Cao Ligen Xiao Ruijiao Zhou Source Type: research

Electroacupuncture Regulates Endoplasmic Reticulum Stress and Ameliorates Neuronal Injury in Rats with Acute Ischemic Stroke
This study aimed to determine whether endoplasmic reticulum stress (ERS) could contribute to the protective effects of EA in cerebral ischemia/reperfusion injury (CIRI) to provide a rationale for the widespread clinical use of EA. Rats were divided into the sham-operated (sham) group, the CIRI (model) group, and the EA group. Rats in the model group were subjected to middle cerebral artery occlusion (MCAO) for 2 h followed by 72 h of reperfusion. Rats with CIRI were treated daily with EA at GV20 and ST36 for a total of 3 days. The Longa scoring system and adhesive removal somatosensory test were applied to evaluate neurolo...
Source: Evidence-based Complementary and Alternative Medicine - August 26, 2021 Category: Complementary Medicine Authors: Ya-Min Zhang Hong Xu Su-Hui Chen Hua Sun Source Type: research

RNA-seq analysis of ischemia stroke and normal brain in a tree shrew model with or without type 2 diabetes mellitus
AbstractNowadays, similar strategies have been used for the treatment and prevention of acute stroke in both diabetes mellitus (DM) and non-DM populations. These strategies were analyzed to provide an experimental basis for the clinical prevention and treatment of stroke in patients both with and without DM. Tree shrews were randomly divided into control, DM, ischemic stroke (IS), and DMIS groups with 18 animals in each group. Serum biochemical indicators were used to assess metabolic status. Neural tissue damage was determined using triphenyl tetrazolium chloride staining, H-E staining, and electron microscopy. Differenti...
Source: Metabolic Brain Disease - August 21, 2021 Category: Neurology Source Type: research

An outer-pore gate modulates the pharmacology of the TMEM16A channel Pharmacology
TMEM16A Ca2+-activated chloride channels are involved in multiple cellular functions and are proposed targets for diseases such as hypertension, stroke, and cystic fibrosis. This therapeutic endeavor, however, suffers from paucity of selective and potent modulators. Here, exploiting a synthetic small molecule with a biphasic effect on the TMEM16A channel, anthracene-9-carboxylic...
Source: Proceedings of the National Academy of Sciences - August 19, 2021 Category: Science Authors: Ria L. Dinsdale, Tanadet Pipatpolkai, Emilio Agostinelli, Angela J. Russell, Phillip J. Stansfeld, Paolo Tammaro Tags: Pharmacology Biological Sciences Source Type: research

Neurovascular protection of salvianolic acid B and ginsenoside Rg1 combination against acute ischemic stroke in rats
Ischemic stroke continues to be a major global health problem associated with considerable mortality and morbidity. Thus, it is still targeted by researchers for developing new strategies or drugs to alleviate the lesion of stroke. In the present study, both the permanent occlusion of the middle cerebral artery (MCAO) model and the restoration of cerebral blood flow after middle cerebral artery occlusion (CI/R) model were set up for evaluating the efficiency of salvianolic acid B and ginsenoside Rg1 combination (SalB-Rg1). SalB-Rg1 decreased infarct area through 3,5-triphenyltetrazolium chloride stain and improved neurolog...
Source: NeuroReport - August 16, 2021 Category: Neurology Tags: Degeneration and Repair Source Type: research

Orexin-A alleviates astrocytic apoptosis and inflammation via Inhibiting OX1R-mediated NF- κB and MAPK signaling pathways in cerebral ischemia/reperfusion injury
Biochim Biophys Acta Mol Basis Dis. 2021 Aug 3:166230. doi: 10.1016/j.bbadis.2021.166230. Online ahead of print.ABSTRACTOrexin-A (OXA) is a neuropeptide with neuroprotective effect by reducing cerebral ischemia/reperfusion injury (CIRI). Inflammation and apoptosis mediated by astrocyte activation are the key pathological mechanisms for CIRI. We thus attempted to confirm neuroprotective effects of OXA on astrocytic inflammation and apoptosis in CIRI and clarify the relative mechanisms. A middle cerebral artery occlusion and reperfusion (MCAO/R) rat model and U251 glioma cells model subjected to oxygen glucose deprivation an...
Source: Biochimica et Biophysica Acta - August 6, 2021 Category: Biochemistry Authors: Dandan Xu Tingting Kong Ziqi Shao Minghui Liu Rumin Zhang Shengnan Zhang Qingxia Kong Jing Chen Baohua Cheng Chunmei Wang Source Type: research

A Single-Center Clinical Study to Evaluate Shenxiong Glucose Injection Combined with Edaravone in the Treatment of Acute Large-Area Cerebral Infarction
CONCLUSIONS: In addition to the routine basic treatment of acute large-area cerebral infarction, the addition of Shenxiong glucose injection combined with edaravone injection can improve platelet aggregation and reduce inflammation by affecting P-selectin, D-dimer, and FIB. This treatment approach promotes the recovery of nerve defect function without obvious adverse reactions in patients with acute large-area cerebral infarction.PMID:34307676 | PMC:PMC8263277 | DOI:10.1155/2021/9935752
Source: Biomed Res - July 26, 2021 Category: Research Authors: Zongqin Li Xiaoxia Rong Jun Luo Tao Zeng Pan Huang Xuejie Xu Source Type: research

Systematic analysis of the mechanism of hydroxysafflor yellow A for treating ischemic stroke based on network pharmacology technology
In this study, we analyzed the mechanism of hydroxysafflor yellow A (HSYA) for treating ischemic stroke (IS) based on network pharmacology tools, and verified the kernel targets via animal experiments. The targets of HSYA were collected via PharmMapper server and the IS-related targets were searched using Genecards, Online Mendelian Inheritance in Man, Therapeutic Target, and Disgenet databases. The targets identified from the above two steps were overlapped to acquire candidate targets involved in the effects of HSYA for treating IS. Subsequently, the Database for Annotation, Visualization, and Integrated Discovery was us...
Source: European Journal of Pharmacology - July 24, 2021 Category: Drugs & Pharmacology Authors: Qian Cui Yu-Hui Ma Hao-Yu Yu Yu-Liang Zhang Xiu-de Qin Shao-Qin Ge Guo-Wei Zhang Source Type: research

Targeting TMEM16A-encoded Ca2+-activated Cl − channels: a new paradigm for antihypertensive therapy?
Hypertension is the leading risk factor for the development of heart diseases and stroke. Many hypertensive patients experience undesirable side effects to conventional antihypertensive pharmacotherapy. Cil et  al. documented the antihypertensive profile of a novel molecule, TMinh-23 (2-bromodifluoroacetylamino-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxylic acid o-tolylamide), in the spontaneously hypertensive rat model of systemic hypertension. They showed that this agent reduces blood pressu re by inhibiting transmembrane member 16A–encoded calcium-activated chloride channels in vascular myocytes from resistance arteries.
Source: Kidney International - July 19, 2021 Category: Urology & Nephrology Authors: Normand Leblanc Tags: Commentary Source Type: research

Novel Human Acute Ischemic Stroke Blood Clot Analogs for In Vitro Thrombectomy Testing INTERVENTIONAL
CONCLUSIONS: Red blood cell–rich, fibrin-rich, and platelet-rich clot analogs that mimic clots retrieved from patients with acute ischemic stroke were created in vitro. Differing retrieval outcomes were confirmed using in vitro thrombectomy testing in a subset of clots.
Source: American Journal of Neuroradiology - July 13, 2021 Category: Radiology Authors: Fitzgerald, S. T., Liu, Y., Dai, D., Mereuta, O. M., Abbasi, M., Larco, J. L. A., Douglas, A. S., Kallmes, D. F., Savastano, L., Doyle, K. M., Brinjikji, W. Tags: INTERVENTIONAL Source Type: research

Electrophilic and Drug-Induced Stimulation of NOTCH3 N-terminal Fragment Oligomerization in Cerebrovascular Pathology
AbstractSmall vessel disease is a prevalent age-related condition linked to increased risk of dementia and stroke. We investigate the most commonly inherited form, CADASIL, caused by cysteine-involving mutations inNOTCH3. Recent studies highlight accumulation of NOTCH3 N-terminal fragmentation product (NTF) in disease. In vitro, NTF is capable of both spontaneous and catecholamine-enhanced cysteine-mediated oligomerization. Despite well-characterized genetic influence on CADASIL, environmental effects, including medication usage, on disease remain unclear. We studied effects of assorted electrophilic compounds and drugs on...
Source: Translational Stroke Research - May 3, 2021 Category: Neurology Source Type: research

Oxytocin improves ischemic stroke by reducing expression of excitatory amino acid transporter 3 in rat MCAO model
This study investigates the neuroprotective effect of OT in an animal model of middle cerebral artery occlusion (MCAO) and the possible role of EAAT3. Transient MCAO was performed as a model of ischemic stroke in male rats and then OT was administrated intra-nasally. Infarct volume was measured by 2, 3, 5-triphenyl tetrazolium chloride staining. Nissl staining method was performed for the evaluation of neuronal cell morphology. Immunohistochemistry assay was performed to analyze the EAAT3 expression in the ischemic region. OT significantly reduced the infarct volume in the cerebral cortex and striatum after ischemia (P<...
Source: Journal of Immunoassay and Immunochemistry - March 31, 2021 Category: Biochemistry Authors: Parinaz Barahimi Mohammad Karimian Majid Nejati Abolfazl Azami Tameh Mohammad Ali Atlasi Source Type: research

LncRNA-MIAT promotes neural cell autophagy and apoptosis in ischemic stroke by up-regulating REDD1
CONCLUSION: Altogether, MIAT promotes autophagy and apoptosis of neural cells and aggravates IS by up-regulating the expression of REDD1.PMID:33745924 | DOI:10.1016/j.brainres.2021.147436
Source: Brain Research - March 22, 2021 Category: Neurology Authors: Xiaqing Guo Yabo Wang Donglin Zheng Xiangshu Cheng Yuhua Sun Source Type: research

Protective Role of Low Ethanol Administration Following Ischemic Stroke via Recovery of KCC2 and p75 NTR Expression
AbstractA striking result from epidemiological studies show a correlation between low alcohol intake and lower incidence for ischemic stroke and severity of derived brain injury. Although reduced apoptosis and inflammation has been suggested to be involved, little is known about the mechanism mediating this effect in vivo. Increase in intracellular chloride concentration and derived depolarizing GABAAR-mediated transmission are common consequences following various brain injuries and are caused by the abnormal expression levels of the chloride cotransporters NKCC1 and KCC2. Downstream pro-apoptotic signaling through p75NTR...
Source: Molecular Neurobiology - February 11, 2021 Category: Neurology Source Type: research

Intracarotid Transplantation of Skin-Derived Precursor Schwann Cells Promotes Functional Recovery After Acute Ischemic Stroke in Rats
Conclusions: Intracarotid transplantation of SKP-SCs promoted functional recovery in the rat AIS model and possesses the potential to be further developed as a novel therapy to treat ischemic stroke in humans.
Source: Frontiers in Neurology - February 4, 2021 Category: Neurology Source Type: research

S-oxiracetam Facilitates Cognitive Restoration after Ischemic Stroke by Activating α7nAChR and the PI3K-Mediated Pathway.
In conclusion, α7nAChR and PI3K are key molecules that mediated the signaling pathway leading to S-ORC-induced cognitive restoration after MCAO/R. PMID: 33481205 [PubMed - as supplied by publisher]
Source: Neurochemical Research - January 22, 2021 Category: Neuroscience Authors: Fan W, Zhang Y, Li X, Xu C Tags: Neurochem Res Source Type: research

Can the early use of botulinum toxin in post stroke spasticity reduce contracture development? A randomised controlled trial.
CONCLUSION: BoNTA reduced spasticity and contractures after stroke and effects lasted for approximately 12-weeks. BoNTA reduced the need for concomitant contracture treatment and did not interfere with recovery of arm function. TRIAL REGISTRATION: EudraCT (2010-021257-39) and ClinicalTrials.gov-Identifier: NCT01882556. PMID: 33040610 [PubMed - as supplied by publisher]
Source: Clinical Rehabilitation - October 11, 2020 Category: Rehabilitation Authors: Lindsay C, Ispoglou S, Helliwell B, Hicklin D, Sturman S, Pandyan A Tags: Clin Rehabil Source Type: research

Responses of immune organs following cerebral ischemic stroke.
CONCLUSIONS: The present results demonstrated that stroke is a systemic disease, which not only affects the brain, but also induces responses of immune organs. On the basis of these results, a systemic treatment might be a good strategy for clinical stroke care. PMID: 32863340 [PubMed - as supplied by publisher]
Source: Journal of Nippon Medical School - August 30, 2020 Category: Universities & Medical Training Authors: Tan C, Wang Z, Zheng M, Zhao S, Shichinohe H, Houkin K Tags: J Nippon Med Sch Source Type: research

Interleukin-1 receptor inhibition reduces stroke size in a murine model of sickle cell disease.
Abstract Sickle cell disease (SCD) is associated with chronic hemolytic anemia and a heightened inflammatory state. The causal role of inflammatory pathways in stroke associated with SCD is unclear. Therefore, the hypothesis that deletion of the non-hematopoietic interleukin-1 receptor (IL-1R) pool may be beneficial in SCD was pursued. Since potential deleterious effects of IL-1R signaling in SCD could be mediated via downstream production of interleukin-6 (IL-6), the role of the non-hematopoietic IL-6 pool was also addressed. Bone marrow transplantation (BMT) from SCD to wild-type (WT) recipient mice was used to ...
Source: Haematologica - August 12, 2020 Category: Hematology Authors: Venugopal J, Wang J, Mawri J, Guo C, Eitzman D Tags: Haematologica Source Type: research

Waggle needling wields preferable neuroprotective and anti-spastic effects on post-stroke spasticity rats by attenuating γ-aminobutyric acid transaminase and enhancing γ-aminobutyric acid.
This study was designed to compare the anti-spastic effect of waggle needling to the routine needling and to explore its underlying mechanism. We established post-stroke spasticity model based on ischemia stroke operation (middle cerebral artery occlusion). Rats were divided into six groups: normal control group, sham-operated control group, ischemia stroke model group, waggle needling group, routine needling group and baclofen group. Neurological function and muscle tone were assessed by the Zea Longa score and modified Ashworth scale, respectively. Indirect muscle tone was testified with electrophysiological recording. C...
Source: NeuroReport - June 9, 2020 Category: Neurology Tags: Degeneration and Repair Source Type: research

Astragaloside IV regulates the HIF/VEGF/Notch signaling pathway through miRNA-210 to promote angiogenesis after ischemic stroke.
CONCLUSIONS: AS-IV promotes cerebral protection following angiogenesis and ischemic brain injury. The specific mechanism was activating the HIF/VEGF/Notch signaling pathway via miRNA-210. PMID: 32417803 [PubMed - as supplied by publisher]
Source: Restorative Neurology and Neuroscience - May 19, 2020 Category: Neurology Tags: Restor Neurol Neurosci Source Type: research

Polyoxometalate nanoclusters act as broad-spectrum antioxidants to alleviate ischemic stroke by intrathecal injection
Conclusion: Intrathecal injection significantly improved the delivery and efficacy of POM nanoclusters to the brain, resulting in the rapid accumulation of POM nanoclusters in the ischemic penumbra. Due to this, we confirmed that the POM nanoclusters could be used as nano-antioxidants to inhibit oxidative stress as well as inflammatory damage effectively.
Source: Journal of Nuclear Medicine - May 14, 2020 Category: Nuclear Medicine Authors: Rosenkrans, Z., Li, S., Jiang, D., NI, D., Engle, J., Cai, W. Tags: Center for Molecular Imaging Innovation & amp; Translation (CMIIT) YIA Symposium Source Type: research

Molecular imaging of inflammation in the brain-heart axis after ischemic stroke: comparison of two murine stroke models.
Conclusions: In contrast to topical ET-1 application after craniotomy, the intravascular MCAo model enables imaging studies of brain-heart networking after stroke, which evokes elevated TSPO PET signal in both brain and heart. The severity of cerebral ischemic damage may contribute to cardiac dysfunction via systemic inflammatory networking.
Source: Journal of Nuclear Medicine - May 14, 2020 Category: Nuclear Medicine Authors: Hermanns, N., Bascunana, P., Langer, B. L. N., Wolf, B., Bankstahl, J., Ross, T., Bengel, F., Thackeray, J. Tags: Cardiovascular Council YIA Symposium Source Type: research

Interleukin-18 from neurons and microglia mediates depressive behaviors in mice with post-stroke depression.
In this study, we demonstrate that in IL-18 level in the ischemic brain significantly increased in mice with depression-like behaviors that caused by the combined use of chronic spatial restraint stress and middle cerebral artery occlusion. Interestingly, IL-18 expression was mainly found in neurons at early phase and in microglia in late phase. Injection of exogenous IL-18 in to the amygdala, but not the hippocampus or the striatum, caused severe depression-like behaviors. On the contrary, the blockage of endogenous IL-18 by IL-18 binding protein, a specific antagonist of IL-18, rescued depressive phenotypes in SIR mice. ...
Source: Brain, Behavior, and Immunity - April 5, 2020 Category: Neurology Authors: Wu D, Zhang G, Zhao C, Yang Y, Miao Z, Xu X Tags: Brain Behav Immun Source Type: research

Renshen Shouwu extract enhances neurogenesis and angiogenesis via inhibition of TLR4/NF-κB/NLRP3 signaling pathway following ischemic stroke in rats
ConclusionsRSSW enhances neurogenesis and angiogenesis via inhibition of TLR4/NF-κB/NLRP3 inflammatory signaling pathway following ischemic stroke in rats. Hence, RSSW may be a promising Chinese Medicine for the treatment of ischemic stroke.Graphical abstract
Source: Journal of Ethnopharmacology - February 10, 2020 Category: Drugs & Pharmacology Source Type: research

Renshen Shouwu extract enhances neurogenesis and angiogenesis via inhibition of TLR4/NF¬κB/NLRP3 signaling pathway following ischemic stroke in rats
ConclusionsRSSW enhances neurogenesis and angiogenesis via inhibition of TLR4/NF¬κB/NLRP3 inflammatory signaling pathway following ischemic stroke in rats. Hence, RSSW may be a promising Chinese Medicine for the treatment of ischemic stroke.Graphical abstract
Source: Journal of Ethnopharmacology - January 31, 2020 Category: Drugs & Pharmacology Source Type: research

Motor deficit in the mouse ferric chloride-induced distal middle cerebral artery occlusion model of stroke.
In this study, we reproduced the recently developed model and expanded its characterization by thorough evaluation of blood supply, cerebral infarction, and motor function in adult male and female mice up to 14 days after stroke. Our observations indicate near complete interruption of blood flow in the distal MCA shortly after application of 20% ferric chloride over the artery through a cranial window, which remained occluded for at least 4 hours. As expected, infarction of the brain tissue, documented by TTC and hematoxylin stains, was restricted to the cerebral cortex. We also systematically evaluated motor impairment ...
Source: Behavioural Brain Research - December 4, 2019 Category: Neurology Authors: Syeara N, Alamri FF, Jayaraman S, Lee P, Karamyan ST, Arumugam TV, Karamyan VT Tags: Behav Brain Res Source Type: research

Intracarotid cold saline infusion contributes to neuroprotection in MCAO ‑induced ischemic stroke in rats via serum and glucocorticoid‑regulated kinase 1.
Intracarotid cold saline infusion contributes to neuroprotection in MCAO‑induced ischemic stroke in rats via serum and glucocorticoid‑regulated kinase 1. Mol Med Rep. 2019 Aug 21;: Authors: Wang D, Huang Z, Li L, Yuan Y, Xiang L, Wu X, Ni C, Yu W Abstract Intracarotid cold saline infusion (ICSI) brings about neuroprotective effects in ischemic stroke. However, the involvement of serum and glucocorticoid‑regulated kinase 1 (SGK1) in the underlying mechanism of ICSI is not fully understood; therefore, we used the rat middle cerebral artery occlusion (MCAO) model to investigate the neuroprotective ...
Source: Molecular Medicine Reports - September 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Pramipexole prevents ischemic cell death via mitochondrial pathways in ischemic stroke RESEARCH ARTICLE
ABSTRACT A dopamine D2 receptor agonist, pramipexole, has been found to elicit neuroprotection in patients with Parkinson’s disease and restless leg syndrome. Recent evidence has shown that pramipexole mediates its neuroprotection through mitochondria. Considering this, we examined the possible mitochondrial role of pramipexole in promoting neuroprotection following an ischemic stroke of rat. Male Wistar rats underwent transient middle cerebral artery occlusion (tMCAO) and then received pramipexole (0.25 mg and 1 mg/kg body weight) at 1, 6, 12 and 18 h post-occlusion. A panel of neurological tests and ...
Source: DMM Disease Models and Mechanisms - August 28, 2019 Category: Biomedical Science Authors: Andrabi, S. S., Ali, M., Tabassum, H., Parveen, S., Parvez, S. Tags: Rat as a Disease Model RESEARCH ARTICLE Source Type: research

Longitudinal investigation of ischemic stroke using magnetic resonance imaging: Animal model.
CONCLUSIONS: The MCAO animal model was successfully established on SD rats using the microsurgery to assess arterial embolization of intracranial tissue injury. PMID: 31306147 [PubMed - as supplied by publisher]
Source: Journal of X-Ray Science and Technology - July 16, 2019 Category: Radiology Tags: J Xray Sci Technol Source Type: research

Bumetanide: A review of its neuroplasticity and behavioral effects after stroke.
Authors: Tao D, Liu F, Sun X, Qu H, Zhao S, Zhou Z, Xiao T, Zhao C, Zhao M Abstract Stroke often leads to neuronal injury and neurological functional deficits. Whilst spontaneous neurogenesis and axon regeneration are induced by ischemic stroke, effective pharmacological treatments are also essential for the improvement of neuroplasticity and functional recovery after stroke. However, no pharmacological therapy has been demonstrated to be able to effectively improve the functional recovery after stroke. Bumetanide is a specific Na +-K +-Cl- co-transporter inhibitor which can maintain chloride homeostasis in neuro...
Source: Restorative Neurology and Neuroscience - July 16, 2019 Category: Neurology Tags: Restor Neurol Neurosci Source Type: research

Effect of TTC Treatment on Immunohistochemical Quantification of Collagen IV in Rat Brains after Stroke
AbstractAlthough used extensively in stroke research, there is limited knowledge of how 2, 3, 5-triphenyltetrazolium chloride (TTC)-treated rat brain sections are altered and if they can be used for immunohistochemical quantification after staining with TTC. In the present study, we hypothesized that TTC treatment (TTC+) would not interfere with collagen IV immunohistochemical staining compared with non-TTC-treated (TTC −) brain slices. We further hypothesized that there would be no difference in autofluorescence or nonspecific secondary antibody fluorescence between TTC+ and TTC− brain slices. Coronal brain sections o...
Source: Translational Stroke Research - October 1, 2018 Category: Neurology Source Type: research

PAI-1 but Not PAI-2 Gene Deficiency Attenuates Ischemic Brain Injury After Experimental Stroke
The objectives of the present were twofold: first, to characterize the time-dependent cerebral mRNA expression of the plasminogen activator system (PAS) after brain ischemia and second, to investigate the impact of PAI-1 and PAI-2 on brain infarct volume using gene-deficient mice. AdultC57Bl/6J mice were subjected to unilateral transient middle cerebral artery occlusion (MCAO) followed by reperfusion for 3, 24, 72, or 120  h. Quantitative PCR revealed that brain mRNA expression levels of the PAS components, and particularly of PAI-1 (237-fold) and PAI-2 (19-fold), peaked at 24 h after stroke. Accordingly, PAI-1 plasma ac...
Source: Translational Stroke Research - July 5, 2018 Category: Neurology Source Type: research

Non-Invasive Multimodality Imaging Directly Shows TRPM4 Inhibition Ameliorates Stroke Reperfusion Injury
In this study, we used multimodal imaging to assess edema formation and quantify the amount of metabolically functional brain salvaged after a rat model of stroke reperfusion. TRPM4 upregulation in endothelium emerges as early as 2  h post-stroke induction. Expression of TRPM4 channel was suppressed directly in vivo by treatment with siRNA; scrambled siRNA was used as a control. T2-weighted MRI suggests that TRPM4 inhibition successfully reduces edema by 30% and concomitantly salvages functionally active brain, measured by18F-FDG-PET. These in vivo imaging results correlate well with post-mortem 2,3,5-triphenyltetrazolium...
Source: Translational Stroke Research - March 22, 2018 Category: Neurology Source Type: research

Effect of TTC Treatment on Immunohistochemical Quantification of Collagen IV in Rat Brains after Stroke
AbstractAlthough used extensively in stroke research, there is limited knowledge of how 2, 3, 5-triphenyltetrazolium chloride (TTC)-treated rat brain sections are altered and if they can be used for immunohistochemical quantification after staining with TTC. In the present study, we hypothesized that TTC treatment (TTC+) would not interfere with collagen IV immunohistochemical staining compared with non-TTC-treated (TTC −) brain slices. We further hypothesized that there would be no difference in autofluorescence or nonspecific secondary antibody fluorescence between TTC+ and TTC− brain slices. Coronal brain sections o...
Source: Translational Stroke Research - January 8, 2018 Category: Neurology Source Type: research