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Identification, characterization, and engineering of glycosylation in thrombolyticsa
Biotechnol Adv. 2023 May 12:108174. doi: 10.1016/j.biotechadv.2023.108174. Online ahead of print.ABSTRACTCardiovascular diseases, such as myocardial infarction, ischemic stroke, and pulmonary embolism, are the most common causes of disability and death worldwide. Blood clot hydrolysis by thrombolytic enzymes and thrombectomy are key clinical interventions. The most widely used thrombolytic enzyme is alteplase, which has been used in clinical practice since 1986. Another clinically used thrombolytic protein is tenecteplase, which has modified epitopes and engineered glycosylation sites, suggesting that carbohydrate modifica...
Source: Biotechnology Advances - May 14, 2023 Category: Biotechnology Authors: Martin Toul Veronika Slonkova Jan Mican Adam Urminsky Maria Tomkova Erik Sedlak David Bednar Jiri Damborsky Lenka Hernychova Zbynek Prokop Source Type: research

COVID-19-Associated Coagulopathy: Role of Vitamins D and K
Curr Pharm Biotechnol. 2022 May 27. doi: 10.2174/1389201023666220527110455. Online ahead of print.ABSTRACTRecent reports show coagulopathy as a potential complication and poorer outcome of coronavirus disease 2019 (COVID-19), especially in those with comorbid conditions such as diabetes and hypertension as thrombosis could result in stroke and heart attacks. Indeed, cardiovascular complications in COVID-19 accounts for 40% of mortality. Although there is no standard treatment protocol or guidelines for COVID-19, it is a common practice to use anti-inflammatory corticosteroids and anti-coagulants, especially for severe COVI...
Source: Current Pharmaceutical Biotechnology - May 31, 2022 Category: Biotechnology Authors: Bruk Getachew Harold E Landis Kebreten F Menaye Yousef Tizabi Source Type: research

Biosynthesis and characterization of a novel fibrinolytic alkaline serine protease from newly isolated Bacillus flexusBF12 for biomedical applications.
CONCLUSION: The Bacillus flexus BF12 fibrinolytic enzyme shows promising potentials in nutraceutical and food fortification applications. The application of fibrinolytic enzymes could prevent CVDs. PMID: 33208066 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Biotechnology - November 16, 2020 Category: Biotechnology Authors: Raj TS, Athimoolam S, Vijayaraghavan P Tags: Curr Pharm Biotechnol Source Type: research

Structural Biology and Protein Engineering of Thrombolytics
Publication date: Available online 2 July 2019Source: Computational and Structural Biotechnology JournalAuthor(s): Jan Mican, Martin Toul, David Bednar, Jiri DamborskyAbstract:Myocardial infarction and ischemic stroke are the most frequent causes of death or disability worldwide. Due to their ability to dissolve blood clots, the thrombolytics are frequently used for their treatment. Improving the effectiveness of thrombolytics for clinical uses is of great interest. The knowledge of the multiple roles of the endogenous thrombolytics and the fibrinolytic system grows continuously. The effects of thrombolytics on the alterat...
Source: Computational and Structural Biotechnology Journal - July 3, 2019 Category: Biotechnology Source Type: research

Staphylokinase enzyme: an overview of structure, function and engineered forms.
CONCLUSION: In this review, we summarized a number of expression systems based on recombinant DNA technology and protein-engineering approaches, which have developed for the production of engineered recombinant SAK molecules with higher fibrinolytic activities and lower antigenicity. PMID: 29424308 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Biotechnology - February 9, 2018 Category: Biotechnology Authors: Vakili B, Nezafat N, Negahdaripour M, Yari M, Zare B, Ghasemi Y Tags: Curr Pharm Biotechnol Source Type: research

A novel thrombolytic and anticoagulant serine protease from Polychaeta, Diopatra sugokai.
This study was aimed at assessing the fibrinolytic and anticoagulation features of a novel serine protease extracted and purified from Diopatra sugokai, a polychaeta that inhabits tidal flats. The purified serine protease was obtained through ammonium sulfate precipitation, affinity chromatography, and ion exchange chromatography. Its molecular size was identified via SDS-PAGE. To characterize its enzymatic activities, protease activity at various pH and temperatures, and in the presence of various inhibitors, was measured via azocasein assay. Its fibrinolytic activity and anticoagulant effect were assessed by fibrin zymog...
Source: Journal of Microbiology and Biotechnology - December 8, 2017 Category: Biotechnology Authors: Kim HJ, Shim KH, Yeon SJ, Shin HS Tags: J Microbiol Biotechnol Source Type: research

The characterization of trans-pecos copperhead (Agkistrodon contortrix pictigaster) venom and isolation of two new dimeric disintegrins.
In this study, we are presenting the first report of the purification, isolation, and partial characterization of two new dimeric disintegrins isolated from the venom of trans-pecos copperhead. PMID: 27068364 [PubMed - as supplied by publisher]
Source: Biologicals : Journal of the International Association of Biological Standardization - April 7, 2016 Category: Biotechnology Authors: Lucena S, Rodríguez-Acosta A, Grilli E, Alfonso A, Goins A, Ogbata I, Walls R, Suntravat M, Uzcátegui NL, Guerrero B, Sánchez EE Tags: Biologicals Source Type: research