• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

A Convenient < i > In Vivo < /i > Model Using Small Interfering RNA Silencing to Rapidly Assess Skeletal Gene Function
by Wen Zhang, Can Liu, Bao Hai, Guohong Du, Hong Wang, Huijie Leng, Yingsheng Xu, Chunli Song It is difficult to study bone in vitro because it contains various cell types that engage in cross-talk. Bone biologically links various organs, and it has thus become increasingly evident that skeletal physiology must be studied in an integrative manner in an intact animal. We developed a model u sing local intraosseous small interfering RNA (siRNA) injection to rapidly assess the effects of a target gene on the local skeletal environment. In this model, 160-g male Sprague-Dawley rats were treated for 1–2 weeks. The left tibia...
Source: PLoS One - November 27, 2016 Category: Biomedical Science Authors: Wen Zhang Source Type: research

LATE-BREAKING ABSTRACT: MUC1 downregulation induces corticosteroid resistance in asthma's in vitro and in vivo models
Conclusion: MUC1 downregulation may lack the anti-inflammatory effects of corticosteroids in Asthma.
Source: European Respiratory Journal - November 7, 2016 Category: Respiratory Medicine Authors: Morell, A., Bel, E., Milara, J., Morcillo, E., Cortijo, J. Tags: 5.1 Airway Pharmacology and Treatment Source Type: research

TET3 Mediates Alterations in the Epigenetic Marker 5hmC and Akt Pathway in Steroid ‐Associated Osteonecrosis
This article is protected by copyright. All rights reserved
Source: Journal of Bone and Mineral Research - August 31, 2016 Category: Orthopaedics Authors: Jie Zhao, Xin ‐long Ma, Jian‐xiong Ma, Lei Sun, Bin Lu, Ying Wang, Guo‐sheng Xing, Yan Wang, Ben‐chao Dong, Li‐yan Xu, Ming‐Jie Kuang, Lin Fu, Hao‐hao Bai, Yue Ma, Wei‐lin Jin Tags: Original Article Source Type: research

Reduced Insulin Receptor Expression Enhances Proximal Tubule Gluconeogenesis
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - June 20, 2016 Category: Biochemistry Authors: Gaurav Pandey, Kripa Shankar, Ekta Makhija, Anil Gaikwad, Carolyn Ecelbarger, Anil Mandhani, Aneesh Srivastava, Swasti Tiwari Tags: Article Source Type: research

The mechanisms and significance of up‐regulation of RhoB expression by hypoxia and glucocorticoid in rat lung and A549 cells
In this study, we investigated the effects of hypoxia or/and GC on the expression and activition of RhoB in the lung of rats and human A549 lung carcinoma cells, and further studied its mechanism and significance. We found that hypoxia and dexamethasone (Dex), a synethic GC, not only significantly increased the expression and activation of RhoB independently but also coregulated the expresion of RhoB in vitro and in vivo. Up‐regulation of RhoB by hypoxia was in part through stabilizing the RhoB mRNA and protein. Inhibiting hypoxia‐activated hypoxia‐inducible transcription factor‐1α (HIF‐1α), c‐Jun N‐termina...
Source: Journal of Cellular and Molecular Medicine - February 24, 2016 Category: Molecular Biology Authors: Gao‐Xiang Huang, Xiao‐Yu Pan, Yi‐Duo Jin, Yan Wang, Xiao‐Lian Song, Chang‐Hui Wang, Yi‐Dong Li, Jian Lu Tags: Original Article Source Type: research

The mechanisms and significance of up ‐regulation of RhoB expression by hypoxia and glucocorticoid in rat lung and A549 cells
In this study, we investigated the effects of hypoxia or/and GC on the expression and activition of RhoB in the lung of rats and human A549 lung carcinoma cells, and further studied its mechanism and significance. We found that hypoxia and dexamethasone (Dex), a synethic GC, not only significantly increased the expression and activation of RhoB independently but also coregulated the expresion of RhoB in vitro and in vivo. Up‐regulation of RhoB by hypoxia was in part through stabilizing the RhoB mRNA and protein. Inhibiting hypoxia‐activated hypoxia‐inducible transcription factor‐1α (HIF‐1α), c‐Jun N‐termina...
Source: Journal of Cellular and Molecular Medicine - February 23, 2016 Category: Molecular Biology Authors: Gao ‐Xiang Huang, Xiao‐Yu Pan, Yi‐Duo Jin, Yan Wang, Xiao‐Lian Song, Chang‐Hui Wang, Yi‐Dong Li, Jian Lu Tags: Original Article Source Type: research

Title: Genistein Disrupts Glucocorticoid Receptor Signaling in Human Uterine Endometrial Ishikawa Cells
Conclusions: Using Ishikawa cells, we observed that exposure to genistein resulted in distinct changes in gene expression and unique differences in the GR transcriptome. Citation: Whirledge S, Senbanjo LT, Cidlowski JA. 2015. Genistein disrupts glucocorticoid receptor signaling in human uterine endometrial Ishikawa cells. Environ Health Perspect 123:80–87; http://dx.doi.org/10.1289/ehp.1408437 Address correspondence to J.A. Cidlowski, NIH/NIEHS, MD F3-07, P.O. Box 12233, Research Triangle Park, NC 27709 USA. Telephone: (919) 541-1564. E-mail: cidlows1@niehs.nih.gov We thank X. Xu (Integrative Bioinformatic...
Source: EHP Research - December 31, 2014 Category: Environmental Health Authors: Web Admin Tags: Research Article January 2015 Source Type: research

P53 Dependent Mitochondrial Permeability Transition Pore Opening Is Required for Dexamethasone‐Induced Death of Osteoblasts
Prolonged or overdose glucocorticoids (GCs) usage is the common cause of osteoporosis. In the present study, we studied the cellular mechanism of dexamethasone (Dex)‐induce osteoblast cell death by focusing on the role of mitochondrial permeability transition pore (mPTP). In cultured osteoblastic MC3T3‐E1 cells, Dex‐induced mPTP opening, which was demonstrated by mitochondrial membrane potential (MPP) decrease, cyclophilin‐D (CyPD)–adenine nucleotide translocator 1 (ANT‐1) mitochondrial complexation and cytochrome C (cyto‐C) release. The mPTP inhibitor sanglifehrin A (SfA) dramatically inhibited Dex‐induced...
Source: Journal of Cellular Physiology - February 25, 2014 Category: Cytology Authors: Yun‐fang Zhen, Guo‐dong Wang, Lun‐qing Zhu, Shi‐ping Tan, Fu‐yong Zhang, Xiao‐zhong Zhou, Xiao‐dong Wang Tags: Original Research Article Source Type: research

Dexamethasone-conjugated polyethylenimine/MIF siRNA complex regulation of particulate matter-induced airway inflammation.
Abstract Inhalation of airborne particulate matter (PM), such as silicon dioxide (SiO2) and titanium dioxide (TiO2), induces acute lung inflammation. siRNA therapy has been proposed as a method to repair acute lung inflammation. To determine whether DEXA-PEI/MIF siRNA contributes to SiO2-induced acute lung inflammation repair, we administered Dexa-PEI/MIF siRNA in SiO2-treated Beas-2b cells and instilled DEXA-PEI-MIF siRNA intratracheally in mice with SiO2-induced acute lung inflammation. Using genetic (MIF mRNA RT-PCR), histological (H&E and PAS) and immunohistochemical (MIF and Muc5ac) analyses, we estimated...
Source: Biomaterials - July 4, 2013 Category: Materials Science Authors: Choi M, Lee M, Rhim T Tags: Biomaterials Source Type: research