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Cyclin B3 is required for metaphase to anaphase transition in oocyte meiosis I
In this study, we explored the role of cyclin B3 in female meiosis by generating Ccnb3 mutant mice via CRISPR/Cas9. Ccnb3 mutant oocytes characteristically arrested at metaphase I (MetI) with normal spindle assembly and lacked enough anaphase-promoting complex/cyclosome (APC/C) activity, which is spindle assembly checkpoint (SAC) independent, to initiate anaphase I (AnaI). Securin siRNA or CDK1 inhibitor supplements rescued the MetI arrest. Furthermore, CCNB3 directly interacts with CDK1 to exert kinase function. Besides, the MetI arrest oocytes had normal development after intracytoplasmic sperm injection (ICSI) or parthe...
Source: Journal of Cell Biology - May 5, 2019 Category: Cytology Authors: Li, Y., Wang, L., Zhang, L., He, Z., Feng, G., Sun, H., Wang, J., Li, Z., Liu, C., Han, J., Mao, J., Li, P., Yuan, X., Jiang, L., Zhang, Y., Zhou, Q., Li, W. Tags: Cell Cycle and Division, Development Articles Source Type: research

Downregulation of gene expression and activity of GRIM-19 affects mouse oocyte viability, maturation, embryo development and implantation
Conclusions GRIM-19 may play important roles in mouse oogenesis and early embryonic development and implantation.
Source: Journal of Assisted Reproduction and Genetics - January 6, 2015 Category: Reproduction Medicine Source Type: research

Semi-quantitative measurement of specific proteins in human cumulus cells using reverse phase protein array
Conclusions: The exquisite sensitivity of RPPA allowed detection of specific proteins in individual CCs. Although the validation of antibodies for RPPA is labour intensive, RRPA is a sensitive and quantitative technique allowing the detection of specific proteins from very small quantities of biological samples. RPPA may be of great interest in clinical diagnostics to predict the oocyte competence prior to transfer of the embryo using robust protein biomarkers expressed by CCs.
Source: Reproductive Biology and Endocrinology - October 22, 2013 Category: Endocrinology Authors: Vincent PuardThibaud TranchantVeronique CadoretChristophe GauthierEric ReiterFabrice GuerifDominique Royere Source Type: research