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Alfalfa dwarf cytorhabdovirus P protein is a local and systemic RNA silencing supressor which inhibits programmed RISC activity and prevents transitive amplification of RNA silencing.
In this study, we show that the phosphoprotein (P) encoded by the negative-sense RNA virus alfalfa dwarf virus (ADV), a species of the genus Cytorhabdovirus, family Rhabdoviridae, is a suppressor of RNA silencing. ADV P has a relatively weak local RSS activity, and does not prevent siRNA accumulation. On the other hand, ADV P strongly suppresses systemic RNA silencing, but does not interfere with the short-distance spread of silencing, which is consistent with its lack of inhibition of siRNA accumulation. The mechanism of suppression appears to involve ADV P binding to RNA-induced silencing complex proteins AGO1 and AGO4 a...
Source: Virus Research - August 15, 2016 Category: Virology Authors: Bejerman N, Mann KS, Dietzgen RG Tags: Virus Res Source Type: research

Silencing of odorant binding protein gene AlinOBP4 by RNAi induces declining electrophysiological responses of Adelphocoris lineolatus to six semiochemicals
Abstract Odorant binding proteins (OBPs) are believed to be important for transporting semiochemicals through the aqueous sensillar lymph to the olfactory receptor cells within the insect antennal sensilla. Here, we injected AlinOBP4‐siRNA into the conjunctivum between prothorax and mesothorax of the lucerne plant bug, Adelphocoris lineolatus and evaluated the silencing of AlinOBP4 by reverse transcription polymerase chain reaction (RT‐PCR) analysis, quantitative real‐time PCR (qPCR) test and electroantennogram (EAG) assay. The combination of RT‐PCR and qPCR analyses revealed that the levels of messenger RNA transc...
Source: Insect Science - July 25, 2016 Category: Biology Authors: Xue ‐Ying Zhang, Xiao‐Qiang Zhu, Shao‐Hua Gu, Yan‐Le Zhou, Song‐Ying Wang, Yong‐Jun Zhang, Yu‐Yuan Guo Tags: ORIGINAL ARTICLE Source Type: research

Silencing of odorant binding protein gene AlinOBP4 by RNAi induce electrophysiological responses declining of Adelphocoris lineolatus to six semiochemicals
This article is protected by copyright. All rights reserved
Source: Insect Science - June 5, 2016 Category: Biology Authors: Xue‐Ying Zhang, Xiao‐Qiang Zhu, Shao‐Hua Gu, Yan‐Le Zhou, Song‐Ying Wang, Yong‐Jun Zhang, Yu‐Yuan Guo Tags: ORIGINAL ARTICLE Source Type: research