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Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses
Cell Host Microbe. 2021 Mar 29:S1931-3128(21)00132-3. doi: 10.1016/j.chom.2021.03.004. Online ahead of print.ABSTRACTArgonaute (AGO) proteins bind small RNAs to silence complementary RNA transcripts, and they are central to RNA interference (RNAi). RNAi is critical for regulation of gene expression and antiviral defense in Aedes aegypti mosquitoes, which transmit Zika, chikungunya, dengue, and yellow fever viruses. In mosquitoes, AGO1 mediates miRNA interactions, while AGO2 mediates siRNA interactions. We applied AGO-crosslinking immunoprecipitation (AGO-CLIP) for both AGO1 and AGO2, and we developed a universal software p...
Source: Cell Host and Microbe - April 1, 2021 Category: Microbiology Authors: Kathryn Rozen-Gagnon Meigang Gu Joseph M Luna Ji-Dung Luo Soon Yi Sasha Novack Eliana Jacobson Wei Wang Matthew R Paul Troels K H Scheel Thomas Carroll Charles M Rice Source Type: research

Viruses, Vol. 11, Pages 540: Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, Aedes aegypti
Failloux Although the role of exogenous small interfering RNA (siRNA) and P-element induced wimpy testis (PIWI)-interacting RNA (piRNA) pathways in mosquito antiviral immunity is increasingly better understood, there is still little knowledge regarding the role of mosquito cellular microRNA (miRNA). Identifying direct interactions between the mosquito miRNAs and the RNA genome of arboviruses and choosing the relevant miRNA candidates to explore resulting antiviral mechanisms are critical. Here, we carried out genomic analyses to identify Aedes aegypti miRNAs that potentially interact with various lineages and genotyp...
Source: Viruses - June 9, 2019 Category: Virology Authors: Yen Chen Sreenu Kohl Failloux Tags: Article Source Type: research

Induction of RNA interference to block Zika virus replication and transmission in the mosquito Aedes aegypti.
This study lays critical groundwork for pursuing ZIKV transmission-blocking strategies that exploit the Ae. aegypti exo-siRNA response for arbovirus suppression in natural populations. PMID: 31103782 [PubMed - as supplied by publisher]
Source: Insect Biochemistry and Molecular Biology - May 15, 2019 Category: Biochemistry Authors: Magalhaes T, Bergren NA, Bennett SL, Borland EM, Hartman DA, Lymperopoulos K, Sayre R, Borlee BR, Campbell CL, Foy BD, Olson KE, Blair CD, Black W, Kading RC Tags: Insect Biochem Mol Biol Source Type: research

IFIT5 Participates in the Antiviral Mechanisms of Rainbow Trout Red Blood Cells
We described a new defense mechanism based on IFIT5 antiviral activity in nucleated RBCs that appeared to contribute to halting the VHSV infection. This finding sheds light into novel antiviral therapeutics to mitigate the economic losses and social impact caused by viral infections in the aquaculture industry. This work broadens the knowledge of fish nucleated RBCs functions and their role in the immune response to viral infections. Ethics Statement Experimental protocols and methods relating to experimental animals were reviewed and approved by the Animal Welfare Body and the Research Ethics Committee at the University...
Source: Frontiers in Immunology - April 15, 2019 Category: Allergy & Immunology Source Type: research

Characterization of the Zika virus induced small RNA response in < i > Aedes aegypti < /i > cells
by Margus Varjak, Claire L. Donald, Timothy J. Mottram, Vattipally B. Sreenu, Andres Merits, Kevin Maringer, Esther Schnettler, Alain Kohl RNA interference (RNAi) controls arbovirus infections in mosquitoes. Two different RNAi pathways are involved in antiviral responses: the PIWI-interacting RNA (piRNA) and exogenous short interfering RNA (exo-siRNA) pathways, which are characterized by the production of virus-derived small RNAs of 25–29 and 21 nucleotides, respectively. The exo-siRNA pathway is considered to be the key mosquito antiviral response mechanism. InAedes aegypti-derived cells, Zika virus (ZIKV)-specific siR...
Source: PLoS Neglected Tropical Diseases - October 17, 2017 Category: Tropical Medicine Authors: Margus Varjak Source Type: research

Role of semaphorin‐1a in the developing visual system of the disease vector mosquito Aedes aegypti
Conclusions: The results of this investigation indicate that Sem1 is required for optic lobe development in A. aegypti and highlight the behavioral importance of a functioning visual system in pre‐adult mosquitoes. Developmental Dynamics, 2014. © 2014 Wiley Periodicals, Inc.
Source: Developmental Dynamics - July 1, 2014 Category: Molecular Biology Authors: Keshava Mysore, Ellen Flannery, Matthew T. Leming, Michael Tomchaney, Lucy Shi, Longhua Sun, Joseph E. O’Tousa, David W. Severson, Molly Duman‐Scheel Tags: Patterns & Phenotypes Source Type: research