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Sodium butyrate protects against rotavirus-induced intestinal epithelial barrier damage by activating AMPK-Nrf2 signaling pathway in IPEC-J2 cells
Int J Biol Macromol. 2022 Dec 21:S0141-8130(22)03130-0. doi: 10.1016/j.ijbiomac.2022.12.219. Online ahead of print.ABSTRACTRotavirus (RV) mainly infects intestinal epithelial cells, which leads to diarrhea in newborn piglets with dysfunction in the intestinal mucosal mechanical barrier. Sodium butyrate (SB) is one of the metabolites excreted by gut microbes. However, the protective effect of SB on RV infection induced intestinal mucosal mechanical barrier injury and its potential mechanism has not been well elucidated. In the present study, IPEC-J2 cells with RV infection was a model of intestinal mucosal mechanical barrie...
Source: International Journal of Biological Macromolecules - December 24, 2022 Category: Biochemistry Authors: Xia Dong Yi Wang Xiaoxiao Zhu Linyuan Shen Lei Chen Lili Niu Mailin Gan Shunhua Zhang Ming Zhang Jun Jiang Daiwen Chen Li Zhu Ye Zhao Source Type: research

Recent advances on therapeutic potentials of gold and silver nanobiomaterials for human viral diseases
Curr Res Chem Biol. 2022;2:100021. doi: 10.1016/j.crchbi.2022.100021. Epub 2022 Feb 1.ABSTRACTViral diseases are prominent among the widely spread infections threatening human well-being. Real-life clinical successes of the few available therapeutics are challenged by pathogenic resistance and suboptimal delivery to target sites. Nanotechnology has aided the design of functionalised and non-functionalised Au and Ag nanobiomaterials through physical, chemical and biological (green synthesis) methods with improved antiviral efficacy and delivery. In this review, innovative designs as well as interesting antiviral activities ...
Source: Herpes - July 11, 2022 Category: Infectious Diseases Authors: Yusuf Oloruntoyin Ayipo Ajibola Abdulahi Bakare Umar Muhammad Badeggi Akeem Adebayo Jimoh Amudat Lawal Mohd Nizam Mordi Source Type: research

Viruses, Vol. 12, Pages 1189: Are Viral Vectors Any Good for RNAi Antiviral Therapy?
m RNA interference (RNAi) represents a novel approach for alternative antiviral therapy. However, issues related to RNA delivery and stability have presented serious obstacles for obtaining good therapeutic efficacy. Viral vectors are capable of efficient delivery of RNAi as short interfering RNA (siRNA), short hairpin RNA (shRNA) and micro-RNA (miRNA). Efficacy in gene silencing for therapeutic applications against viral diseases has been demonstrated in various animal models. Rotavirus (RV) miR-7 can inhibit rotavirus replication by targeting the RV nonstructural protein 5. Viral gene silencing by targeting the RNAi ...
Source: Viruses - October 20, 2020 Category: Virology Authors: Kenneth Lundstrom Tags: Editorial Source Type: research

High-Throughput Characterization of Viral and Cellular Protein Expression Patterns During JC Polyomavirus Infection
Discussion The study of viral infections in vitro has provided innumerable advances to the field of virology. However, the lack of rapid and efficient screening tools has hindered research progress for some viruses, like JCPyV (Houff et al., 1983; Zu Rhein, 1983; Assetta and Atwood, 2017). To overcome this challenge, the development of high-throughput analyses is needed to help aid in the production of large data sets and generation of multiple lines of inquiry. Current methodologies for analyzing JCPyV infectivity predominantly rely on manual quantitation of infection by indirect immunodetection of viral proteins by epif...
Source: Frontiers in Microbiology - April 16, 2019 Category: Microbiology Source Type: research

Profiling of rotavirus 3'UTR-binding proteins reveals the ATP synthase subunit ATP5B as a host factor that supports late-stage virus replication Microbiology
In this study, we employed a proteomics-based approach, named RNA–protein interaction detection (RaPID), to comprehensively screen for host proteins that bind to a conserved motif within the rotavirus (RV) 3′ terminus. Using this assay, we identified ATP5B, a core subunit of the mitochondrial ATP synthase, as having high affinity to the RV 3′UTR consensus sequences. During RV infection, ATP5B bound to the RV 3′UTR and co-localized with viral RNA and viroplasm. Functionally, siRNA-mediated genetic depletion of ATP5B or other ATP synthase subunits such as ATP5A1 and ATP5O reduced the production of infectious viral pr...
Source: Journal of Biological Chemistry - April 11, 2019 Category: Chemistry Authors: Lili Ren, Siyuan Ding, Yanhua Song, Bin Li, Muthukumar Ramanathan, Julia Co, Manuel R. Amieva, Paul A. Khavari, Harry B. Greenberg Tags: Microbiology Source Type: research

Downregulation of Hes1 expression in experimental biliary atresia and its effects on bile duct structure.
CONCLUSION: Our data indicated that Hes1 might contribute to the maturation and the cellular structure organization of biliary epithelial cells, which provides new insight into understanding the pathology of biliary atresia. PMID: 30090006 [PubMed - in process]
Source: World Journal of Gastroenterology - August 7, 2018 Category: Gastroenterology Authors: Zhang RZ, Zeng XH, Lin ZF, Ming-Fu, Tong YL, Lui VC, Tam PK, Lamb JR, Xia HM, Chen Y Tags: World J Gastroenterol Source Type: research

Glucose enhances rotavirus enterotoxin-induced intestinal chloride secretion.
We examined the effects of NSP4 and glucose on sodium and chloride transport in mouse small intestines and Caco-2 cells. Mouse small intestines and Caco-2 cells were incubated with NSP4114-135 in the presence/absence of glucose. Absorption and secretion of sodium and chloride, fluid movement, peak amplitude of intracellular calcium fluorescence, and expression of Ano1 and sodium-glucose cotransporter 1 were assessed. NHE3 activity increased, and chloride secretory activity decreased with age. Net chloride secretion increased, and net sodium absorption decreased in the intestines of 3-week-old mice compared to 8-week-old mi...
Source: Pflugers Archiv : European Journal of Physiology - May 10, 2017 Category: Physiology Authors: Yin L, Menon R, Gupta R, Vaught L, Okunieff P, Vidyasagar S Tags: Pflugers Arch Source Type: research