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Molecular methods to investigate adhesion, transmigration, invasion and intracellular survival of the foodborne pathogen Campylobacter jejuni
Publication date: October 2013 Source:Journal of Microbiological Methods, Volume 95, Issue 1 Author(s): Steffen Backert , Dirk Hofreuter Campylobacter jejuni is a spiral-shaped Gram-negative pathogen and major agent of gastrointestinal foodborne illness in humans worldwide. This pathogen encodes numerous described pathogenicity-associated factors involved in important processes including bacterial adhesion to, transmigration across, invasion into and intracellular survival within intestinal epithelial cells. This review article highlights various molecular techniques applied in the studies of each of these individual ste...
Source: Journal of Microbiological Methods - November 4, 2014 Category: Microbiology Source Type: research

The focal complex of epithelial cells provides a signalling platform for interleukin‐8 induction in response to bacterial pathogens
We examined the interaction of several bacterial pathogens with host cells, including Campylobacter jejuni, to determine if the FC is required for induction of chemokine signalling in response to bacterial pathogens. Our data indicate that secretion of IL‐8 is triggered by C. jejuni, Helicobacter pylori and Salmonella enterica serovar Typhimurium in response to engagement of β1 integrins. Additionally, we found that the secretion of IL‐8 from C. jejuni infected epithelial cells requires FAK, Src and paxillin, which in turn are necessary for Erk 1/2 recruitment and activation. Targeting the FC component paxillin with s...
Source: Cellular Microbiology - June 2, 2014 Category: Microbiology Authors: Tyson P. Eucker, Derrick R. Samuelson, Mary Hunzicker‐Dunn, Michael E. Konkel Tags: Research Article Source Type: research

The focal complex of epithelial cells provides a signaling platform for interleukin‐8 induction in response to bacterial pathogens
We examined the interaction of several bacterial pathogens with host cells, including Campylobacter jejuni, to determine if the FC is required for induction of chemokine signaling in response to bacterial pathogens. Our data indicate that secretion of IL‐8 is triggered by C. jejuni, Helicobacter pylori, and Salmonella enterica serovar Typhimurium in response to engagement of β1 integrins. Additionally, we found that the secretion of IL‐8 from C. jejuni infected epithelial cells requires FAK, Src, and paxillin, which in turn are necessary for Erk 1/2 recruitment and activation. Targeting the FC component paxillin with ...
Source: Cellular Microbiology - April 29, 2014 Category: Microbiology Authors: Tyson P. Eucker, Derrick R. Samuelson, Mary Hunzicker‐Dunn, Michael E. Konkel Tags: Research Article Source Type: research

Invasion of epithelial cells by Campylobacter jejuni is independent of caveolae
: Caveolae are 25-100 nm flask-like membrane structures enriched in cholesterol and glycosphingolipids. Researchers have proposed that Campylobacter jejuni require caveolae for cell invasion based on the finding that treatment of cells with the cholesterol-depleting compounds filipin III or methyl-beta-cyclodextrin (MbetaCD) block bacterial internalization in a dose-dependent manner. The purpose of this study was to determine the role of caveolae and caveolin-1, a principal component of caveolae, in C. jejuni internalization. Consistent with previous work, we found that the treatment of HeLa cells with MbetaCD inhibited C....
Source: Cell Communication and Signaling - December 23, 2013 Category: Molecular Biology Authors: Michael KonkelDerrick SamuelsonTyson EuckerEric SheldenJason O'Loughlin Source Type: research

Serine phosphorylation of cortactin is required for maximal host cell invasion by Campylobacter jejuni
Conclusion: We conclude that CiaD is involved in the activation of Erk 1/2 and that activated Erk 1/2 facilitates C. jejuni invasion by phosphorylation of cortactin on serine 405 and 418. This is the first time that cortactin and N-WASP have been shown to be involved in C. jejuni invasion of host cells. These data also provide a mechanistic basis for the requirement of Erk 1/2 in C. jejuni-mediated cytoskeletal rearrangement.
Source: Cell Communication and Signaling - November 4, 2013 Category: Molecular Biology Authors: Derrick SamuelsonMichael Konkel Source Type: research