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Molecules, Vol. 27, Pages 1936: A Comprehensive Computational Investigation into the Conserved Virulent Proteins of Shigella species Unveils Potential Small-Interfering RNA Candidates as a New Therapeutic Strategy against Shigellosis
In this study, we analyzed 264 conserved sequences from 15 different conserved virulence genes of Shigella sp., through extensive rational validation using a plethora of first-generation and second-generation computational algorithms for siRNA designing. Fifty-eight siRNA candidates were obtained by using the first-generation algorithms, out of which only 38 siRNA candidates complied with the second-generation rules of siRNA designing. Further computational validation showed that 16 siRNA candidates were found to have a substantial functional efficiency, out of which 11 siRNA candidates were found to be non-immunogenic. Fi...
Source: Molecules - March 17, 2022 Category: Chemistry Authors: Parag Palit Farhana Tasnim Chowdhury Namrata Baruah Bonoshree Sarkar Sadia Noor Mou Mehnaz Kamal Towfida Jahan Siddiqua Zannatun Noor Tahmeed Ahmed Tags: Article Source Type: research

NOD2 and TLR2 Signal via TBK1 and PI31 to Direct Cross-Presentation and CD8 T Cell Responses
The objective of this study was to explore the role of NOD2 and TLR2 in cross-presentation in human dendritic cells undertaking an unbiased screen. We have used a quantitative phosphoproteomic analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) followed by a computational analysis to identify the proteins as differentially abundant in response to NOD2 and TLR2 sensing. Validation of the phosphoproteomic analysis was performed by the detection of proteins in phosphoenriched lysates and detected by western blot. Techniques for the modulation of gene expression (shRNA and siRNA) were used to confirm the resu...
Source: Frontiers in Immunology - April 29, 2019 Category: Allergy & Immunology Source Type: research

Double-stranded RNA induces cathelicidin expression in the intestinal epithelial cells through phosphatidylinositol 3-kinase-protein kinase C ζ-Sp1 pathway and ameliorates shigellosis in mice.
This study reports novel regulatory mechanisms of cathelicidin expression that may be targeted to treat gastro-intestinal infections. PMID: 28343946 [PubMed - as supplied by publisher]
Source: Cellular Signalling - March 24, 2017 Category: Cytology Authors: Ta A, Thakur BK, Dutta P, Sinha R, Koley H, Das S Tags: Cell Signal Source Type: research

Novel Host Proteins and Signaling Pathways in Enteropathogenic E. coli Pathogenesis Identified by Global Phosphoproteome Analysis.
Abstract Enteropathogenic Escherichia coli (EPEC) uses a type III secretion system (T3SS) to directly translocate effector proteins into host cells where they play a pivotal role in subverting host cell signaling needed for disease. However, our knowledge of how EPEC affects host protein phosphorylation is limited to a few individual protein studies. We employed a quantitative proteomics approach to globally map alterations in the host phosphoproteome during EPEC infection. By characterizing host phosphorylation events at various time points throughout infection, we examined how EPEC dynamically impacts the host p...
Source: Molecular and Cellular Proteomics : MCP - May 5, 2015 Category: Molecular Biology Authors: Scholz R, Imami K, Scott NE, Trimble WS, Foster LJ, Finlay BB Tags: Mol Cell Proteomics Source Type: research

Shigella subverts the host recycling compartment to rupture its vacuole.
Abstract Shigella enters epithlial cells via internalization into a vacuole. Subsequent vacuolar membrane rupture allows bacterial escape into the cytosol for replication and cell-to-cell spread. Bacterial effectors such as IpgD, a PI(4,5)P2 phosphatase that generates PI(5)P and alters host actin, facilitate this internalization. Here, we identify host proteins involved in Shigella uptake and vacuolar membrane rupture by high-content siRNA screening and subsequently focus on Rab11, a constituent of the recycling compartment. Rab11-positive vesicles are recruited to the invasion site before vacuolar rupture, and Ra...
Source: Cell Host and Microbe - October 8, 2014 Category: Microbiology Authors: Mellouk N, Weiner A, Aulner N, Schmitt C, Elbaum M, Shorte SL, Danckaert A, Enninga J Tags: Cell Host Microbe Source Type: research

Dynamin-related protein Drp1 and mitochondria are important for Shigella flexneri infection.
In this study the role of the mitochondrial fission protein, Drp1 during Shigella infection in HeLa cells was examined. Significant lactate dehydrogenase (LDH) release was detected in the culture supernatant when HeLa cells were infected with Shigella at a high multiplicity of infection. Drp1 inhibition with Mdivi-1 and siRNA knockdown significantly reduced LDH release. HeLa cell death was also accompanied by mitochondrial fragmentation. Tubular mitochondrial networks were partially restored when Drp1 was depleted with either siRNA or inhibited with Mdivi-1. Surprisingly either Mdivi-1 treatment or Drp1 siRNA-depletion of ...
Source: International Journal of Medical Microbiology - April 3, 2014 Category: Microbiology Authors: Lum M, Morona R Tags: Int J Med Microbiol Source Type: research