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Source: Autophagy
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Total 127 results found since Jan 2013.
ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1
Autophagy. 2023 Jul 11:1-16. doi: 10.1080/15548627.2023.2233846. Online ahead of print.ABSTRACTBaf A1: bafilomycin A1; GABARAP: GABA type A receptor-associated protein; GFP: green fluorescent protein; IFN: interferon; IKBKE/IKKi: inhibitor of nuclear factor kappa B kinase subunit epsilon; IRF3: interferon regulatory factor 3; ISG: interferon-stimulated gene; ISRE: IFN-stimulated response element; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MAVS: mitochondrial antiviral signaling protein; MOI: multiplicity of infection; PAMPs: pathogen-associated molecule patterns; RIGI/DDX58: RNA sensor RIG-I; SeV: Sendai ...
Source: Autophagy - July 12, 2023 Category: Cytology Authors: Weihong Xie Chenqiu Zhang Zheyu Wang Hui Chen Tonghui Gu Tao Zhou Yaoxing Wu Fan Xia Min Li Jun Wang Renjie Jiao Jun Cui Shouheng Jin Source Type: research
The autophagic protein FYCO1 controls TNFRSF10/TRAIL receptor induced apoptosis and is inactivated by CASP8 (caspase 8)
Autophagy. 2023 Jul 7:1-19. doi: 10.1080/15548627.2023.2229656. Online ahead of print.ABSTRACTApoptosis is a tightly controlled cell death program executed by proteases, the so-called caspases. It plays an important role in tissue homeostasis and is often dysregulated in cancer. Here, we identified FYCO1, a protein that promotes microtubule plus end-directed transport of autophagic and endosomal vesicles as a molecular interaction partner of activated CASP8 (caspase 8). The absence of FYCO1 sensitized cells to basal and TNFSF10/TRAIL-induced apoptosis by receptor accumulation and stabilization of the Death Inducing Signali...
Source: Autophagy - July 7, 2023 Category: Cytology Authors: Valeria Coppola Ilaria Marino Uwe Warnken Mario Falchi Luca Pasquini Mauro Biffoni Ruggero De Maria Tobias Longin Haas Source Type: research
High-resolution visualization and assessment of basal and OXPHOS-induced mitophagy in H9c2 cardiomyoblasts
In this study, we visualized and assessed the level of mitophagy in H9c2 cardiomyoblasts at basal conditions and after OXPHOS induction by galactose adaptation. We used cells with a stable expression of a pH-sensitive fluorescent mitochondrial reporter and applied state-of-the-art imaging techniques and image analysis. Our data showed a significant increase in acidic mitochondria after galactose adaptation. Using a machine-learning approach we also demonstrated increased mitochondrial fragmentation by OXPHOS induction. Furthermore, super-resolution microscopy of live cells enabled capturing of mitochondrial fragments withi...
Source: Autophagy - July 5, 2023 Category: Cytology Authors: Gustav Godtliebsen Kenneth Bowitz Larsen Zambarlal Bhujabal Ida S Opstad Mireia Nager Abhinanda R Punnakkal Trine B Kalstad Randi Olsen Trine Lund Dilip K Prasad Krishna Agarwal Truls Myrmel Asa Birna Birgisdottir Source Type: research
Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration
This study validates a protective role of Krt8 during overloading-induced IDD and demonstrates that targeting overloading activation of PKNs could be a novel and effective approach to mechano stress-induced pathologies with a wider window of therapeutic opportunity.Abbreviations: AAV: adeno-associated virus; AF: anulus fibrosus; ANOVA: analysis of variance; ATG: autophagy related; BSA: bovine serum albumin; cDNA: complementary deoxyribonucleic acid; CEP: cartilaginous endplates; CHX: cycloheximide; cKO: conditional knockout; Cor: coronal plane; CT: computed tomography; Cy: coccygeal vertebra; D: aspartic acid; DEG: differe...
Source: Autophagy - March 10, 2023 Category: Cytology Authors: Di Wang Qiliang Shang Jianxin Mao Chu Gao Jie Wang Dong Wang Han Wang Haoruo Jia Pandi Peng Mu Du Zhuojing Luo Liu Yang Source Type: research
N protein of PEDV plays chess game with host proteins by selective autophagy
In this study, we have found a novel host restriction factor, HNRNPA1, that could inhibit PEDV replication by degrading viral nucleocapsid (N) protein. The restriction factor activates the HNRNPA1-MARCHF8/MARCH8-CALCOCO2/NDP52-autophagosome pathway with the help of transcription factor EGR1 targeting the HNRNPA1 promoter. HNRNPA1 could also promote the expression of IFN to facilitate the host antiviral defense response for antagonizing PEDV infection through RIGI protein interaction. During viral replication, we found that PEDV can, in contrast, degrade the host antiviral proteins HNRNPA1 and others (FUBP3, HNRNPK, PTBP1, ...
Source: Autophagy - March 2, 2023 Category: Cytology Authors: Xueying Zhai Ning Kong Yu Zhang Yiyi Song Wenzhen Qin Xinyu Yang Chenqian Ye Manqing Ye Wu Tong Changlong Liu Hao Zheng Hai Yu Wen Zhang Xia Yang Gaiping Zhang Guangzhi Tong Tongling Shan Source Type: research
The COPS3-FOXO3 positive feedback loop regulates autophagy to promote cisplatin resistance in osteosarcoma
Autophagy. 2022 Nov 30:1-18. doi: 10.1080/15548627.2022.2150003. Online ahead of print.ABSTRACTChemotherapy is an important treatment modality for osteosarcoma (OS), but the development of chemoresistance limits the therapeutic efficacy of OS and results in a poor prognosis. Thus, a better understanding of the mechanisms underlying chemoresistance in OS is essential. We previously demonstrated that COPS3/CSN3 (COP9 signalosome subunit 3) functions as an oncogene to promote OS cells lung metastasis, which is closely related to chemoresistance. Here, we showed that COPS3 was significantly upregulated in OS tissues with poor ...
Source: Autophagy - December 1, 2022 Category: Cytology Authors: Jianfang Niu Taiqiang Yan Wei Guo Wei Wang Tingting Ren Yi Huang Zhiqing Zhao Yiyang Yu Chenglong Chen Qingshan Huang Jingbing Lou Lei Guo Source Type: research
How cells recognize and remove the perforated lysosome
Autophagy. 2022 Nov 11:1-3. doi: 10.1080/15548627.2022.2138686. Online ahead of print.ABSTRACTMacroautophagy (hereafter autophagy) is a highly conserved intracellular degradation system to maintain cellular homeostasis by degrading cellular components such as misfolded proteins, nonfunctional organelles, pathogens, and cytosol. Conversely, selective autophagy targets and degrades specific cargo, such as organelles, bacteria, etc. We previously reported that damaged lysosomes are autophagy targets, via a process called lysophagy. However, how cells target damaged lysosomes through autophagy is not known. We performed proteo...
Source: Autophagy - November 11, 2022 Category: Cytology Authors: Keisuke Tabata Marika Saeki Tamotsu Yoshimori Maho Hamasaki Source Type: research
