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The protective effect of Palmatine on depressive like behavior by modulating microglia polarization in LPS-induced mice
In conclusion, this research demonstrated that Palmatine attenuated depressive like behavior by modulating microglia polarization via PDE4B/KLF4 signaling.PMID:35917005 | DOI:10.1007/s11064-022-03672-3
Source: Neurochemical Research - August 2, 2022 Category: Neuroscience Authors: Lei Wang Min Li Cuiping Zhu Aiping Qin Jinchun Wang Xianni Wei Source Type: research

Antidepressants Fluoxetine Mediates Endoplasmic Reticulum Stress and Autophagy of Non –Small Cell Lung Cancer Cells Through the ATF4-AKT-mTOR Signaling Pathway
This study mainly focused on the discovery of the molecular basis of the inhibitory effect of fluoxetine in lung cancer. The specific anti-proliferation effect and autophagy induced by fluoxetine on lung cancer cell were shown in CCK8 and immunofluorescence. The RNA sequence hinted that the endoplasmic reticulum (ER) stress-related protein and mTOR pathway were enriched after fluoxetine treatment. Western blot results revealed that the ER stress pathway was activated by fluoxetine, including PERK, ATF4, and CHOP, while the AKT/mTOR pathway was inhibited. In addition, the transfection of ATF4 siRNA further discovered that E...
Source: Frontiers in Pharmacology - May 10, 2022 Category: Drugs & Pharmacology Source Type: research

Aging-Dependent Downregulation of SUV39H1 Histone Methyltransferase Increases Susceptibility to Stress-Induced Depressive Behavior
AbstractAging induces cellular and molecular changes including gene expression alteration in the brain, which might be associated with aging-induced decrease in stress coping ability. In the present study, we investigate how aging changes the ability to cope with stress and increases sensitivity to stress. Aged mice show decreased expression of SUV39H1 histone methyltransferase and increased expression of Mkp-1 in the hippocampus. The siRNA-mediated knockdown of SUV39H1 increases Mkp-1 expression and suppresses p-CREB and Bdnf expression in HT22 cells and in the hippocampus of mice. Chromatin immunoprecipitation assays ind...
Source: Molecular Neurobiology - September 18, 2021 Category: Neurology Source Type: research

Saikosaponin d downregulates microRNA-155 and upregulates FGF2 to improve depression-like behaviors in rats induced by unpredictable chronic mild stress by negatively regulating NF- κB.
Saikosaponin d downregulates microRNA-155 and upregulates FGF2 to improve depression-like behaviors in rats induced by unpredictable chronic mild stress by negatively regulating NF-κB. Brain Res Bull. 2020 Jan 08;: Authors: Chao B, Huang S, Pan J, Zhang Y, Wang Y Abstract Saikosaponin d (SSd) is a traditional Chinese medicine that has been widely used in depression treatment. Given the lack of studies demonstrating the underlying mechanism of action of SSd in depression, the presented study was conducted with aims of investigating the effect of SSd on rats with depression-like behaviors induced by un...
Source: Brain Research Bulletin - January 7, 2020 Category: Neurology Authors: Chao B, Huang S, Pan J, Zhang Y, Wang Y Tags: Brain Res Bull Source Type: research

Fluoxetine induces autophagic cell death via eEF2K ‐AMPK‐mTOR‐ULK complex axis in triple negative breast cancer
ConclusionsThese results demonstrate that Fluoxetine induces apoptosis and autophagic cell death in TNBC, which will hold a promise for the future TNBC therapy.
Source: Cell Proliferation - October 1, 2017 Category: Cytology Authors: Dejuan Sun, Lingjuan Zhu, Yuqian Zhao, Yingnan Jiang, Lixia Chen, Yang Yu, Liang Ouyang Tags: ORIGINAL ARTICLE Source Type: research

Fluoxetine induces apoptosis through endoplasmic reticulum stress via mitogen-activated protein kinase activation and histone hyperacetylation in SK-N-BE(2)-M17 human neuroblastoma cells
In this study, the molecular mechanisms underlying the anti-cancer effects of FLX were investigated in SK-N-BE(2)-M17 human neuroblastoma cells. FLX induced apoptotic cell death, activation of caspase-4, -9, and -3, and expression of endoplasmic reticulum (ER) stress-associated proteins, including C/EBP homologous protein (CHOP). Inhibition of ER stress by treatment with the ER stress inhibitors, salubrinal and 4-phenylbutyric acid or CHOP siRNA transfection reduced FLX-induced cell death. FLX induced phosphorylation of mitogen-activated protein kinases (MAPKs) family, p38, JNK, and ERK, and an upstream kinase apoptosis si...
Source: Apoptosis - June 24, 2017 Category: Molecular Biology Source Type: research

Galanin (1-15) enhancement of the behavioral effects of Fluoxetine in the forced swimming test gives a new therapeutic strategy against depression.
We examined the ability of the neuropeptide Galanin(1-15) [GAL(1-15)] to modulate the behavioral effects of FLX in the forced swimming test (FST) and studied feasible molecular mechanisms. The data show that GAL(1-15) enhances the antidepressant-like effects induced by FLX in the FST, and we demonstrate the involvement of GALR1/GALR2 heteroreceptor complex in the GAL(1-15)-mediated effect using in vivo rat models for siRNA GALR1 or GALR2 knockdown. Importantly, 5-HT1A receptors (5HT1AR) also participate in the GAL(1-15)/FLX interactions since the 5HT1AR antagonist WAY100635 blocked the behavioral effects in the FST induced...
Source: Neuropharmacology - March 9, 2017 Category: Drugs & Pharmacology Authors: Flores-Burgess A, Millón C, Gago B, Narváez M, Borroto-Escuela DO, Mengod G, Narváez JA, Fuxe K, Santín L, Díaz-Cabiale Z Tags: Neuropharmacology Source Type: research

Antidepressants activate the lysophosphatidic acid receptor LPA1 to induce insulin-like growth factor-I receptor transactivation, stimulation of ERK1/2 signaling and cell proliferation in CHO-K1 fibroblasts.
Abstract Different lines of evidence indicate that the lysophosphatidic acid (LPA) receptor LPA1 is involved in neurogenesis, synaptic plasticity and anxiety-related behaviour, but little is known on whether this receptor can be targeted by neuropsychopharmacological agents. The present study investigated the effects of different antidepressants on LPA1 signaling. We found that in Chinese hamster ovary (CHO)-K1 fibroblasts expressing endogenous LPA1 tricyclic and tetracyclic antidepressants and fluoxetine induced the phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2) and CREB. This response was a...
Source: Biochemical Pharmacology - April 15, 2015 Category: Drugs & Pharmacology Authors: Olianas MC, Dedoni S, Onali P Tags: Biochem Pharmacol Source Type: research

Chronic fluoxetine administration increases expression of the L-channel gene Cav1.2 in astrocytes from the brain of treated mice and in culture and augments K(+)-induced increase in Ca(2+)i.
Abstract We have recently shown that freshly isolated astrocytes from the mouse brain express mRNA for the L-channel gene Cav1.3 to at least the same degree (per mg mRNA) as corresponding neurons. The amount of extracellular Ca(2+) actually entering cultured astrocytes by its opening is modest, but due to secondary Ca(2+)-mediated stimulation of the ryanodine receptor (RyR) the increase in free cytosolic Ca(2+) [Ca(2+)]i is substantial. The other Cav1 subtype expressed in brain is Cav1.2, which is even expressed in higher density. Although the different primers used for the two genes preclude exact quantitative co...
Source: Cell Calcium - January 22, 2014 Category: Cytology Authors: Du T, Liang C, Li B, Hertz L, Peng L Tags: Cell Calcium Source Type: research