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Effects of Type 1 Insulin-Like Growth Factor Receptor Silencing in a Human Adrenocortical Cell Line
Horm Metab ResDOI: 10.1055/s-0042-108196Type 1 insulin-like growth factor receptor (IGF-1R) is overexpressed in a variety of human cancers, including adrenocortical tumors. The aim of the work was to investigate the effects of IGF-1R downregulation in a human adrenocortical cell line by small interfering RNA (siRNA). The human adrenocortical tumor cell line NCI H295R was transfected with 2 specific IGF1R siRNAs (# 1 and # 2) and compared with untreated cells and a negative control siRNA. IGF1R expression was determined by quantitative reverse-transcription PCR (qRTPCR) and Western blot. The effects of IGF-1R downregulation...
Source: Hormone and Metabolic Research - May 30, 2016 Category: Endocrinology Authors: Ribeiro, T. C.Jorge, A. A.Montenegro, L. R.Almeida, M. Q.Ferraz-de-Souza, B.Nishi, M. Y.Mendonca, B. B.Latronico, A. C. Tags: Endocrine Research Source Type: research

In Situ Functionalized Polymers for siRNA Delivery.
Abstract A new method is reported herein for screening the biological activity of functional polymers across a consistent degree of polymerization and in situ, that is, under aqueous conditions and without purification/isolation of candidate polymers. In brief, the chemical functionality of a poly(acryloyl hydrazide) scaffold was activated under aqueous conditions using readily available aldehydes to obtain amphiphilic polymers. The transport activity of the resulting polymers can be evaluated in situ using model membranes and living cells without the need for tedious isolation and purification steps. This tec...
Source: Angewandte Chemie - April 20, 2016 Category: Chemistry Authors: Priegue JM, Crisan DN, Martínez-Costas J, Granja JR, Fernandez-Trillo F, Montenegro J Tags: Angew Chem Int Ed Engl Source Type: research

Dynamic Amphiphile Libraries To Screen for the “Fragrant” Delivery of siRNA into HeLa Cells and Human Primary Fibroblasts
Journal of the American Chemical SocietyDOI: 10.1021/ja404153m
Source: Journal of the American Chemical Society - June 14, 2013 Category: Chemistry Authors: Charlotte Gehin, Javier Montenegro, Eun-Kyoung Bang, Ana Cajaraville, Shota Takayama, Hisaaki Hirose, Shiroh Futaki, Stefan Matile and Howard Riezman Source Type: research