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New perspectives in triple-negative breast cancer therapy based on treatments with TGF β1 siRNA and doxorubicin.
In this study, a combined doxorubicin (DOX) and small interfering RNA (siRNA) therapy is proposed as therapeutic strategy for targeting TGFβ1 gene. Hs578T cell line is used as in vitro model for TNBC, wherein TGFβ1siRNA therapy is employed to enhance therapeutic effects. Cell proliferation rate is measured using an MTT test, and morphological alterations are assed using microscopically approached, while gene expression is determined by qRT-PCR analysis. The combined treatment of TGFβ1siRNA and DOX reduced levels of cell proliferation and mitochondrial activity and promoted the alteration of cell morphology (dark-field m...
Source: Molecular and Cellular Biochemistry - September 3, 2020 Category: Biochemistry Authors: Ciocan-Cȃrtiţă CA, Jurj A, Raduly L, Cojocneanu R, Moldovan A, Pileczki V, Pop LA, Budişan L, Braicu C, Korban SS, Berindan-Neagoe I Tags: Mol Cell Biochem Source Type: research

PIG-a Gene Expression Deficiency Association with Reduced DNA Damage Checkpoint Response and Activation
Conclusion:The above-mentioned results showed that there were decreased expressions of both DNA damage response checkpoint genes and repair genes in both the PIG-A CRISPR knockout leukemia cell lines as well as in the PNH patients. PIG-A mutation is globally associated with reduced DNA damage response capability and increased cellular stability. Our finding explains, at least partially, why PIG-A gene mutation status could be seen as a biomarker of mutagenesis and how PNH cells dominantly expend via clonal escape.DisclosuresNo relevant conflicts of interest to declare.
Source: Blood - November 21, 2018 Category: Hematology Authors: Pu, J. J., Lu, S., Nemesure, M., Teye, E. K., Schleicher, E., Moldovan, G.-L., Brodsky, R. A., Chen, F. Tags: 508. Bone Marrow Failure: Poster III Source Type: research

RNase L restricts the mobility of engineered retrotransposons in cultured human cells
Retrotransposons are mobile genetic elements, and their mobility can lead to genomic instability. Retrotransposon insertions are associated with a diverse range of sporadic diseases, including cancer. Thus, it is not a surprise that multiple host defense mechanisms suppress retrotransposition. The 2',5'-oligoadenylate (2-5A) synthetase (OAS)-RNase L system is a mechanism for restricting viral infections during the interferon antiviral response. Here, we investigated a potential role for the OAS-RNase L system in the restriction of retrotransposons. Expression of wild type (WT) and a constitutively active form of RNase L (N...
Source: Nucleic Acids Research - April 2, 2014 Category: Research Authors: Zhang, A., Dong, B., Doucet, A. J., Moldovan, J. B., Moran, J. V., Silverman, R. H. Tags: Nucleic Acid Enzymes Source Type: research