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Cancer: Choriocarcinoma

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Total 20 results found since Jan 2013.

HAND1 knockdown disrupts trophoblast global gene  expression
In this study, we aimed to build upon this knowledge and characterize the mechanistic impacts of disruptingHAND1 on human placenta trophoblast and vascular endothelial cell gene expression.HAND1 gene expression was silenced in BeWo cells, a choriocarcinoma model of human cytotrophoblasts, (n = 3–9 passages) and isolated human placental microvascular endothelial cells (HPMVEC;n = 3 passages), withHAND1 siRNA for 96  h. Cells were harvested, mRNA isolated and RNA sequencing performed using the Illumina NextSeq 550 platform. Normalization and differential gene expression analyses were conducted using general linear mo...
Source: Physiological Reports - January 25, 2023 Category: Physiology Authors: Robert Fresch, Jennifer Courtney, Heather Brockway, Rebecca L. Wilson, Helen Jones Tags: ORIGINAL ARTICLE Source Type: research

Dexamethasone may inhibit placental growth by blocking glucocorticoid receptors via phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin and reactive oxygen species/AMP-activated protein kinase signalling pathways in human placental JEG-3 cells
This study explored the molecular mechanism underlying the effects of dexamethasone (DEX, 1µM) on glucose transporters (GLUT) in JEG-3 human placental choriocarcinoma cells. JEG-3 cells were treated with DEX, an expression plasmid encoding human glucocorticoid receptor α (GRα), pcDNA3.1-GRα, GRα short interference (si) RNA, LY294002, xanthine oxidase (XO)/hypoxanthine (HX), rapamycin, insulin-like growth factor (IGF) 1, N-acetylcysteine (NAC) or phosphatidic acid (PA), and cell proliferation, apoptosis, mitochondrial membrane potential (MMP), human chorionic gonadotrophin (hCG) content, human placental lactogen (hPL) ...
Source: Reproduction, Fertility, and Development - August 16, 2021 Category: Reproduction Medicine Authors: Xin Zhan Yiran Xie Liping Sun Qi Si Hongkai Shang Source Type: research

Disruption of p97/VCP induces autophagosome accumulation, cell cycle arrest and apoptosis in human choriocarcinoma cells
Mol Biol Rep. 2021 Feb 23. doi: 10.1007/s11033-021-06225-z. Online ahead of print.ABSTRACTGestational choriocarcinoma is aggressive trophoblastic disease. The development, progression and the cure of this disease is not well-established. p97/Valosin containing protein has been shown to play critical roles in many cellular processes. In various cancers, higher expression of p97/VCP has been reported and targeting of p97/VCP with its spesific inhibitors or siRNA's (siVCP) in cancer therapy was suggested. However, no study is avaible about the expression and function of p97/VCP in gestational choriocarcinoma. Hence, the aim o...
Source: Molecular Biology Reports - February 23, 2021 Category: Molecular Biology Authors: Raziye Desdicioglu Cansu Sahin Filiz Yavuz Sevil Cayli Source Type: research

MicroRNA-126 suppresses the invasion of trophoblast-model JEG-3 cells by targeting LIN28A.
Abstract Inadequate trophoblast invasion and impaired trophoblast-induced vascular remodeling are features of preeclampsia. In this context, an angiogenesis-related microRNA, miR-126, is abnormally expressed in preeclampsia placentas, but its role in trophoblast development remains unclear. The purpose of this study was to investigate the roles of miR-126 in the proliferation, migration, and invasion processes of trophoblast cells using the human choriocarcinoma-derived JEG-3 cell line as a model. The mRNA expression profiling of JEG-3 cells with and without miR-126 overexpression, in combination with bioinforma...
Source: Biochemical and Biophysical Research communications - February 3, 2021 Category: Biochemistry Authors: Pan X, Noguchi S, Ando M, Nishimura T, Tomi M Tags: Biochem Biophys Res Commun Source Type: research

Current Development of siRNA Bioconjugates: From Research to the Clinic
In this study, it was shown that the main factor determining the nature of the biodistribution of conjugates is their lipophilicity. Conjugates of siRNA with lower lipophilicity; i.e., derivatives of retinoic acid, lithocholic acid, and docosahexanoic acid with greater efficiency than cholesterol conjugates accumulated in the kidneys, bladder, and lungs of the mouse after subcutaneous injection (Biscans et al., 2018). This fact is consistent with previous data that showed that more lipophilic conjugates bind more efficiently to serum components, and thus are not excreted by the kidneys (Wolfrum et al., 2007; Osborn et al.,...
Source: Frontiers in Pharmacology - April 25, 2019 Category: Drugs & Pharmacology Source Type: research

Versican silencing in BeWo cells and its implication in gestational trophoblastic diseases
AbstractVersican is a proteoglycan known to interact with cells to influence their ability to proliferate, differentiate, migrate, invade and assemble extracellular matrix, with all of these cell functions present during placentation. In the placenta, cytotrophoblast cells have the ability to differentiate into the syncytiotrophoblast, a mechanism that is greatly increased in gestational trophoblastic diseases (GTD). Nevertheless, the molecular signaling underlying the increased syncytiotrophoblast differentiation are still being unveiled and may result in novel therapeutic targets for GTD. Versican expression was investig...
Source: Histochemistry and Cell Biology - October 8, 2018 Category: Biomedical Science Source Type: research

Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.
Abstract Embryonic development depends on the normal invasion of trophoblast cells. Forkhead box C2 (FOXC2) is a member of Forkhead box family, which is involved in the tumor cells invasion. The aim of this study was to explore the roles of FOXC2 on the invasion of human trophoblast cells, and further study its molecular mechanism. The mRNA and protein levels of FOXC2 in human normal trophoblast and choriocarcinoma cell lines were analyzed by quantitative real-time PCR (qRT-PCR) and western blot assays, respectively. Methylthiazolyldiphenyl-tetrazo lium bromide (MTT) and transwell assays were separately performed ...
Source: Cell Biology International - February 21, 2018 Category: Cytology Authors: Zhang Y, Zhang Y Tags: Cell Biol Int Source Type: research

Involvement of the DNA mismatch repair system in cisplatin sensitivity of testicular germ cell tumours
ConclusionThis study reports, for the first time, expression of the MMR system in fetal gonocytes, from which GCNIS cells are derived. Our findings in primary TGCT specimens and TGCT-derived cells suggest that a reduced sensitivity to cisplatin in differentiated TGCT components could result from a reduced expression of MMR proteins, in particular MSH2 and MLH1, which are involved in the recognition of cisplatin adducts and in activation of the DNA damage response pathway to initiate apoptosis.
Source: Cellular Oncology - May 23, 2017 Category: Cancer & Oncology Source Type: research

Effect of Histone Deacetylase Inhibition on the Expression of Multidrug Resistance-associated Protein 2 in a Human Placental Trophoblast Cell Line.
CONCLUSIONS: HDACs inhibition could up-regulate placental MRP2 expression in vitro, and HDAC1 was probably to be involved in this process. PMID: 28524836 [PubMed - in process]
Source: Chinese Medical Journal - May 21, 2017 Category: General Medicine Authors: Duan HY, Ma D, Zhou KY, Wang T, Zhang Y, Li YF, Wu JL, Hua YM, Wang C Tags: Chin Med J (Engl) Source Type: research

CLIC1 induces drug resistance in human choriocarcinoma through positive regulation of MRP1.
In conclusion, our results suggest that CLIC1 may serve as a critical mediator of chemoresistance in human choriocarcinoma JeG3 cells. The CLIC1-mediated chemoresistance is achieved through positive regulation of MRP1. Depletion of either CLIC1 or its downstream MRP1 may be a promising therapeutic strategy concerning reversing the chemo-resistance in human choriocarcinoma JeG3 cells. PMID: 27983917 [PubMed - as supplied by publisher]
Source: Oncology Research - December 18, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Suppression of StarD7 promotes endoplasmic reticulum stress and induces ROS production.
Abstract StarD7 is an intracellular lipid transport protein identified as up-regulated in the choriocarcinoma JEG-3 cell line. StarD7 facilitates the delivery of phosphatidylcholine (PC) to the mitochondria, and StarD7 knockdown causes a reduction in phospholipid synthesis. Since inhibition of PC synthesis may lead to endoplasmic reticulum (ER) stress we hypothesized that StarD7 may be involved in maintaining cell homeostasis. Here, we examined the effect of StarD7 silencing on ER stress response and on the levels of reactive oxygen species (ROS) in the human hepatoma cell line HepG2. StarD7 knockdown induced alte...
Source: Free Radical Biology and Medicine - August 19, 2016 Category: Biology Authors: Flores-Martín J, Reyna L, Ridano ME, Panzetta-Dutari GM, Genti-Raimondi S Tags: Free Radic Biol Med Source Type: research

Mercury toxicokinetics of the healthy human term placenta involve amino acid transporters and ABC transporters.
CONCLUSION: The amino acid transporters located at the apical side of the syncytiotrophoblast (STB) manage uptake of MeHg. Mercury conjugated to glutathione (GSH) is effluxed via MRP1 localized to the basal side of the STB. The findings can well explain why mercury is transported primarily towards the fetal side. PMID: 26740192 [PubMed - as supplied by publisher]
Source: Toxicology - December 28, 2015 Category: Toxicology Authors: Straka E, Ellinger I, Balthasar C, Scheinast M, Schatz J, Szattler T, Bleichert S, Saleh L, Knöfler M, Zeisler H, Hengstschläger M, Rosner M, Salzer H, Gundacker C Tags: Toxicology Source Type: research

Altered folate metabolism modifies cell proliferation and progesterone secretion in human placental choriocarcinoma JEG-3 cells.
Abstract Folate is an essential B vitamin required for de novo purine and thymidylate synthesis, and for the remethylation of homocysteine to form methionine. Folate deficiency has been associated with placenta-related pregnancy complications, as have SNP in genes of the folate-dependent enzymes, methionine synthase (MTR) and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1). We aimed to determine the effect of altered folate metabolism on placental cell proliferation, viability and invasive capacity and on progesterone and human chorionic gonadotropin (hCG) secretion. Human placental choriocarcinoma (JEG-3) cell...
Source: The British Journal of Nutrition - August 24, 2015 Category: Nutrition Authors: Moussa C, Ross N, Jolette P, MacFarlane AJ Tags: Br J Nutr Source Type: research

Decreased STAT3 in human idiopathic fetal growth restriction contributes to trophoblast dysfunction
In conclusion, we report that decreased STAT3 expression in placentae may contribute to abnormal trophoblast function in idiopathic FGR-affected pregnancies.
Source: Reproduction - March 30, 2015 Category: Reproduction Medicine Authors: Borg, A. J., Yong, H. E. J., Lappas, M., Degrelle, S. A., Keogh, R. J., Da Silva-Costa, F., Fournier, T., Abumaree, M., Keelan, J. A., Kalionis, B., Murthi, P. Tags: Research Source Type: research