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Transitional Insight into the RNA-Based Oligonucleotides in Cancer Treatment
Appl Biochem Biotechnol. 2023 Jul 4. doi: 10.1007/s12010-023-04597-5. Online ahead of print.ABSTRACTConventional cancer therapies with chemodrugs suffer from various disadvantages, such as irreversible side effects on the skin, heart, liver, and nerves with even fatal consequences. RNA-based therapeutic is a novel technology which offers great potential as non-toxic, non-infectious, and well-tolerable platform. Herein, we introduce different RNA-based platforms with a special focus on siRNA, miRNA, and mRNA applications in cancer treatment in order to better understand the details of their therapeutic effects. Of note, the...
Source: Applied Biochemistry and Biotechnology - July 4, 2023 Category: Biochemistry Authors: Hamed Tabasi Samaneh Mollazadeh Elham Fazeli Khalil Abnus Seyed Mohammad Taghdisi Mohammad Ramezani Mona Alibolandi Source Type: research

Cancers, Vol. 12, Pages 1023: Knockdown of UTX/KDM6A Enriches Precursor Cell Populations in Urothelial Cell Cultures and Cell Lines
olfgang A. Schulz The histone demethylase UTX (gene: KDM6A) directs cell and tissue differentiation during development. Deleterious mutations in KDM6A occur in many human cancers, most frequently in urothelial carcinoma. The consequences of these mutations are poorly understood; plausibly, they may disturb urothelial differentiation. We therefore investigated the effects of UTX siRNA-mediated knockdown in two in vitro models of urothelial differentiation; namely, primary cultures of urothelial epithelial cells treated with troglitazone and PD153035 and the immortalized urothelial cell line HBLAK treated with high calci...
Source: Cancers - April 20, 2020 Category: Cancer & Oncology Authors: Alexander Lang Patcharawalai Whongsiri Merve Yilmaz Tobias Lautwein Patrick Petzsch Annemarie Greife Cagatay G ünes Karl K öhrer G ünter Niegisch Mich èle Hoffmann Wolfgang A. Schulz Tags: Article Source Type: research

NEK4 kinase regulates EMT to promote lung cancer metastasis.
Abstract Epithelial-to-mesenchymal transition (EMT) is a dynamic transitional state from the epithelial to mesenchymal phenotypes. Numerous studies have suggested that EMT and its intermediate states play important roles in tumor invasion and metastasis. To identify novel regulatory molecules of EMT, we screened a siRNA library targeting human 720 kinases in A549 lung adenocarcinoma cells harboring E-cadherin promoter-luciferase reporter vectors. NIMA-related kinase-4 (NEK4) was identified and characterized as a positive regulator of EMT in the screening. Suppression of NEK4 resulted in the inhibition of cell migr...
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Ding NH, Zhang L, Xiao Z, Rong ZX, Li Z, He J, Chen L, Ou DM, Liao WH, Sun LQ Tags: J Cell Mol Med Source Type: research

Downregulation of feline sarcoma-related protein inhibits cell migration, invasion and epithelial-mesenchymal transition via the ERK/AP-1 pathway in bladder urothelial cell carcinoma.
Authors: Hu X, Zhang Z, Liang Z, Xie D, Zhang T, Yu D, Zhong C Abstract Feline sarcoma-related protein (Fer) is a nuclear and cytoplasmic non-receptor protein tyrosine kinase and Fer overexpression is associated with various biological processes. However, the clinicopathological characteristics and molecular mechanisms of Fer expression in bladder urothelial cell carcinoma (UCC) have yet to be elucidated. The present study demonstrated that Fer was significantly upregulated in bladder UCC tissues and cell lines. A clinicopathological analysis suggested that Fer expression was significantly associated with tumor sta...
Source: Oncology Letters - March 31, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

MicroRNA-497 inhibits the proliferation, migration and invasion of human bladder transitional cell carcinoma cells by targeting E2F3.
Authors: Zhang Y, Zhang Z, Li Z, Gong D, Zhan B, Man X, Kong C Abstract Accumulating evidence indicates that microRNAs (miRNAs) play critical roles in regulating cellular processes, such as cell growth and apoptosis, as well as cancer progression and metastasis. Low expression of miR-497 has been observed in breast, colorectal and cervical cancers. Human bladder transitional cell carcinoma (BTCC) progression typically follows a complex cascade from primary malignancy to distant metastasis, but whether the aberrant expression of miR-497 in BTCC is associated with malignancy, metastasis or prognosis remains unknown. ...
Source: Oncology Reports - July 21, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

MiR-429 reverses epithelial-mesenchymal transition by restoring E-cadherin expression in bladder cancer.
Authors: Wu CL, Ho JY, Chou SC, Yu DS Abstract Epithelial-mesenchymal transition (EMT) accompanying loss of E-cadherin is important for invasiveness and metastasis of bladder cancer. MicroRNAs (miRs) had been associated with cancer progression and differentiation in several cancers. Our goal is to find out the specific miR which modulates EMT in bladder cancer. Real-time quantitative polymerase chain reaction was used to measure the miRs expression in urothelial cell carcinoma (UCC) cell lines. MiR or siRNA mimics was used to regulate miR and mRNA level respectively. Migration and scratch assays were used to determ...
Source: Oncotarget - April 9, 2016 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Downregulation of fatty acid synthase complex suppresses cell migration by targeting phospho‑AKT in bladder cancer.
Authors: Zheng SS, Gao JG, Liu ZJ, Zhang XH, Wu S, Weng BW, Wang YL, Hou SC, Jiang B Abstract The aim of the present study was to investigate the effect of fatty acid synthase complex (FASN) on the migration capacity of bladder transitional cell carcinoma (BTCC) cells and the involvement of matrix metalloproteinase‑9 (MMP‑9) via targeting of phospho‑AKT (p‑AKT). FASN‑specific small‑interfering RNA (FASN‑siRNA) was used to inhibit FASN gene expression in the 5637 and 253J BTCC cell lines. The knockdown efficiency of FAM‑conjugated FASN‑siRNA was confirmed by fluorescence microscopy. The migratory a...
Source: Molecular Medicine Reports - January 16, 2016 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Abstract 1724: Integrated functional RNAi screening and structural genomics identify inverse co-modulators of TP53 family and NF-{kappa}B transitional activation as potential therapeutic targets in head and neck squamous cell carcinoma
Head and neck squamous cell carcinoma (HNSCC) is the 6th most common cancer worldwide with a 50-60% mortality rate. Deregulation of p53 family members in HNSCC occurs in over 90% of cases, preventing transcription of growth arrest and apoptosis genes. Conversely, members of the NF-κB/REL family are aberrantly activated in about ∼70% of cases, and drive expression of pro-proliferation, inflammation, angiogenesis, and therapeutic resistance genes. The function of different TP53 and NF-κB family members are inversely modulated within two major subsets of HNSCC, suggesting that common molecules and pathways coordinate this...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Saleh, A., Cornelius, S., Martin, S., Ormanoglu, P., Cheng, H., Das, R., Yang, X., Chen, Z., Van Waes, C. Tags: Experimental and Molecular Therapeutics Source Type: research

High expression of KPNA2 defines poor prognosis in patients with upper tract urothelial carcinoma treated with radical nephroureterectomy
Conclusions: KPNA2 is a novel independent prognostic marker for bladder recurrence, DFS and OS of UTUC patients who have undergone RNU. Moreover, these data suggest that KPNA2 may be a promising therapeutic target for UTUC.
Source: BMC Cancer - May 9, 2015 Category: Cancer & Oncology Authors: Bentao ShiBoxing SuDong FangYuan TangGengyan XiongZhongqiang GuoQun HeXinyu YangWei ZhaoYinglu GuoXuesong LiLiqun Zhou Source Type: research

SiRNA knockdown of the DEK nuclear protein mRNA enhances apoptosis and chemosensitivity of canine transitional cell carcinoma cells
Publication date: Available online 12 February 2015 Source:The Veterinary Journal Author(s): Hiroki Yamazaki , Tomomi Iwano , Saori Otsuka , Yumiko Kagawa , Yuki Hoshino , Kenji Hosoya , Masahiro Okumura , Satoshi Takagi Transitional cell carcinoma (TCC) in dogs is an aggressive malignant neoplasm originating in the epithelium of the urinary bladder. The DEK nuclear protein is overexpressed in several types of human bladder cancer, where it is involved in chromatin reconstruction, gene transcription and apoptosis. Since DEK represents a potential therapeutic target for canine TCC, this study was designed to investigate D...
Source: The Veterinary Journal - February 13, 2015 Category: Veterinary Research Source Type: research

Intravenous siRNA silencing of survivin enhances activity of mitomycin C in human bladder RT4 xenografts
Survivin inhibits apoptosis and enables tumor cells to escape from therapy-induced senescence. High expression of survivin is associated with bladder cancer aggressiveness and recurrence. The present study evaluated if survivin expression is reduced by siRNA and if survivin silencing enhances the activity of mitomycin C (MMC), in human RT4 bladder transitional cell tumors in vitro and in vivo.
Source: The Journal of Urology - February 10, 2015 Category: Urology & Nephrology Authors: Minjian Cui, Jessie L.-S. Au, M. Guillaume Wientjes, Michael A. O’Donnell, Kevin R. Loughlin, Ze Lu Source Type: research

Abstract 5239: MiR-148a promotes apoptosis in urothelial cell carcinoma of the bladder cells in part by targeting DNMT1
Conclusions: Our work demonstrates for the first time that miR-148a plays a tumor suppressive role in bladder cancer and suggests it could be used as a novel therapeutic agent either alone or in conjunction with chemotherapeutics. Citation Format: Alan Lombard, Ben Mooso, Steve Libertini, Rebecca Lim, Nicole Costanzo, Paramita Ghosh, Maria Mudryj. MiR-148a promotes apoptosis in urothelial cell carcinoma of the bladder cells in part by targeting DNMT1. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Lombard, A., Mooso, B., Libertini, S., Lim, R., Costanzo, N., Ghosh, P., Mudryj, M. Tags: Molecular and Cellular Biology Source Type: research

Phenotypic impact of deregulated expression of class I histone deacetylases in urothelial cell carcinoma of the bladder
ABSTRACT Deregulated expression of histone deacetylases (HDACs) has been implicated in tumorigenesis. Herein, we investigated class I HDACs expression in bladder urothelial cell carcinoma (BUCC), its prognostic value and biological significance. Significantly increased transcript levels of all HDACs were found in BUCC compared to 20 normal mucosas, and these were higher in lower grade and stage tumors. Increased HDAC3 levels were associated with improved patient survival. SiRNA experiments showed decrease cell viability and motility, and increased apoptosis. We concluded that class I HDACs play an important role in bladder...
Source: Molecular Carcinogenesis - November 30, 2013 Category: Molecular Biology Authors: Susana Junqueira‐Neto, Filipa Q. Vieira, Diana Montezuma, Natália R. Costa, Luís Antunes, Tiago Baptista, Ana Isabel Oliveira, Inês Graça, Ângelo Rodrigues, José S. Magalhães, Jorge Oliveira, Rui Henrique, Carmen Jerónimo Tags: Research Article Source Type: research