Query: siRNA

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Total 18571 results found since Jan 2013.

Prolyl isomerase Pin1 binds to and stabilizes acetyl CoA carboxylase 1 protein, thereby supporting cancer cell proliferation.
Authors: Ueda K, Nakatsu Y, Yamamotoya T, Ono H, Inoue Y, Inoue MK, Mizuno Y, Matsunaga Y, Kushiyama A, Sakoda H, Fujishiro M, Takahashi SI, Matsubara A, Asano T Abstract The prolyl isomerase Pin1 expression level is reportedly increased in most malignant tissues and correlates with poor outcomes. On the other hand, acetyl CoA carboxylase 1 (ACC1), the rate limiting enzyme of lipogenesis is also abundantly expressed in cancer cells, to satisfy the demand for the fatty acids (FAs) needed for rapid cell proliferation. We found Pin1 expression levels to correlate positively with ACC1 levels in human prostate cancers, ...
Source: Oncotarget - March 23, 2019 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Knockdown of serpin peptidase inhibitor clade C member  1 inhibits the growth of nasopharyngeal carcinoma cells.
Knockdown of serpin peptidase inhibitor clade C member 1 inhibits the growth of nasopharyngeal carcinoma cells. Mol Med Rep. 2019 Mar 14;: Authors: Xu J, Ying Y, Xiong G, Lai L, Wang Q, Yang Y Abstract Nasopharyngeal carcinoma (NPC) is a type of cancer originating in the nasopharynx. There are no NPC‑specific treatments available at present. Serpin peptidase inhibitor clade C member 1 (SERPINC1) serves roles in anticoagulation and anti‑inflammation. The aim of the present study was to investigate the role of SERPINC1 in the proliferation and apoptosis of NPC cells. Tumor and adjacent healthy ti...
Source: Molecular Medicine Reports - March 23, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Age-dependent expression of the Vitamin D receptor and the protective effect of Vitamin D receptor activation on H2O2-induced apoptosis in rat intervertebral disc cells
Publication date: Available online 21 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Tong Tong, Zhihui Liu, Hua Zhang, Jinglei Sun, Dongxia Zhang, Feng Wang, Dechao Miao, Yong ShenAbstractAccumulating evidence shows that genetic polymorphism of the vitamin D receptor (VDR) gene is associated with intervertebral disc degeneration (IDD), implying that VDR may be involved in the pathogenesis of IDD. However, the exact relationship between VDR and IDD remains unknown. The aim of this study was to investigate the age-dependent expression of VDR in rat intervertebral discs and to determine ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 22, 2019 Category: Biochemistry Source Type: research

O-GlcNAcylation disrupts STRA6-retinol signals in kidneys of diabetes
ConclusionsO-GlcNAcylation significantly modified STRA6 and RALDH1, suppressed RBP4 binding activity, and disrupted retinol signals in the kidney of diabetes.General significanceThis study first indicates that STRA6-retinol signals were directly disrupted by O-GlcNAcylation in diabetic kidney.
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 22, 2019 Category: Biochemistry Source Type: research

Cardiac myocyte-specific expression of beta3-adrenergic receptors sustains AMPK activation and glucose uptake while reducing hypertrophy following pressure overload
ConclusionWe conclude that cardiac myocyte-specific expression of B3AR concurrently prevents the development of insulin resistance and maintains AMPK activation contributing to the protection from hypertrophic remodeling under hemodynamic stress.
Source: Archives of Cardiovascular Diseases Supplements - March 22, 2019 Category: Cardiology Source Type: research

Modulation of GSK - 3β/β - catenin cascade by commensal bifidobateria plays an important role for the inhibition of metaflammation-related biomarkers in response to LPS or non-physiological concentrations of fructose: An in vitro study
ConclusionWe describe a regulatory mechanism in which commensal bifidobacteria prevent LPS- and/or fructose-mediated transcriptional activation of metabolic disorder-causing factors (IL-1β, IL-6, TNF-α) and disruption of normal cellular energy metabolism by attenuating NF-kB activity and interfering with the Wnt/β-catenin pathway. The latter positively regulates NF-κB activity, thereby simultaneously reducing cellular triglyceride concentrations and enhancing cellular glucose consumption. This may be a molecular basis by which commensal bifidobacteria enhance intrinsic cellular tolerance against excess consumption of e...
Source: PharmaNutrition - March 22, 2019 Category: Nutrition Source Type: research

Cancers, Vol. 11, Pages 406: Alpha6-Integrin Regulates FGFR1 Expression through the ZEB1/YAP1 Transcription Complex in Glioblastoma Stem Cells Resulting in Enhanced Proliferation and Stemness
Catherine SEVA Glioblastoma (GBM) is the most lethal primary brain tumor in adults and is known to be particularly aggressive and resistant to anti-cancer therapies, mainly due to the presence of GBM stem cells (GBMSC). By in vitro approaches supported by analysis from patients’ databases, we determined how α6-integrin and Fibroblast Growth Factor Receptor 1 (FGFR1) work in concert to regulate proliferation and stemness of GBMSC. We showed that α6-integrin regulates the expression of FGFR1 and its target gene Fokhead Box M1 (FOXM1) via the ZEB1/YAP1 transcription complex. Thes...
Source: Cancers - March 22, 2019 Category: Cancer & Oncology Authors: Aline KOWALSKI-CHAUVEL Valerie GOUAZE-ANDERSSON Laurent BARICAULT Elodie MARTIN Caroline DELMAS Christine TOULAS Elizabeth COHEN-JONATHAN-MOYAL Catherine SEVA Tags: Article Source Type: research

FABP4 silencing ameliorates hypoxia reoxygenation injury through the attenuation of endoplasmic reticulum stress-mediated apoptosis by activating PI3K/Akt pathway
Publication date: Available online 21 March 2019Source: Life SciencesAuthor(s): Tianming Deng, Yanhong Wang, Chongchong Wang, Hua YanAbstractEndoplasmic reticulum (ER) stress and subsequent apoptosis play a vital role in myocardial ischemia reperfusion (IR) injury. Fatty acid binding protein 4 (FABP4) may induce ER stress. The aim of this study was to investigate the mechanism and effect of FABP4 on IR injury in vitro. Rat H9c2 cells were exposed to hypoxia reoxygenation (HR) to create an IR model in vitro. FABP4 was overexpressed in HR-injured H9c2 cells. Transfection with FABP4 siRNA increased cell viability and decrease...
Source: Life Sciences - March 21, 2019 Category: Biology Source Type: research

Z-Guggulsterone attenuates glucocorticoid-induced osteoporosis through activation of Nrf2/HO-1 signaling
Publication date: Available online 21 March 2019Source: Life SciencesAuthor(s): Yier Xu, Jian Guan, Jianyu Xu, Shuilin Chen, Guicai SunAbstractAimsThe present study aims to investigate the protective effect and underlying mechanism of Z-Guggulsterone (Z-GS), an active component from myrrh, on glucocorticoid-induced osteoporosis (GIO).Main methodsGIO rats were used to simulate osteoporosis in vivo while MC3T3-E1 cells were induced to osteoblast differentiation and treated with dexamethasone to simulate osteoporosis in vitro. The rats and cells were treated with Z-GS according to the protocol. The bone mineral density, biome...
Source: Life Sciences - March 21, 2019 Category: Biology Source Type: research

Co-Delivery of Gemcitabine and Mcl-1 SiRNA via Cationic Liposome-Based System Enhances the Efficacy of Chemotherapy in Pancreatic Cancer
Source: Journal of Biomedical Nanotechnology - March 21, 2019 Category: Nanotechnology Authors: Wang, Yanbing Gao, Fenghua Jiang, Xingwei Zhao, Xiao Wang, Yu Kuai, Qiyuan Nie, Guangjun He, Min Pan, Yingjie Shi, Wei Ren, Suping Yu, Qun Tags: Articles Source Type: research

DNA tetrahedron-based nanogels for siRNA delivery and gene silencing.
Abstract DNA tetrahedra are employed as building blocks to construct a novel DNA-based nanogel for intracellular siRNA delivery. With a compact architecture that embeds functional siRNA inside, the self-assembled nanogel can efficiently enter cells and knock down the target gene expression, making them potential noncationic vectors for functional nucleic acid delivery. PMID: 30896698 [PubMed - as supplied by publisher]
Source: Chemical Communications - March 21, 2019 Category: Chemistry Authors: Xue H, Ding F, Zhang J, Guo Y, Gao X, Feng J, Zhu X, Zhang C Tags: Chem Commun (Camb) Source Type: research

Tuftelin Is Required for NGF-Induced Differentiation of PC12 Cells
AbstractNerve growth factor (NGF) promotes pleiotropic gene transcription-dependent biological effects, in neuronal and non-neuronal cells, including survival, proliferation, differentiation, neuroprotection, pain, and angiogenesis. It is hypothesized that during odontogenesis, NGF may be implicated in morphogenetic and mineralization events by affecting proliferation and/or differentiation of dental cells. Tuftelin belongs to the enamel associated teeth proteins and is thought to play a role in enamel mineralization. We previously reported that tuftelin transcript and protein, which are ubiquitously expressed in various t...
Source: Journal of Molecular Neuroscience - March 21, 2019 Category: Neuroscience Source Type: research

OTOR in breast carcinoma as a potent prognostic predictor correlates with cell proliferation, migration and invasion.
This study aimed to investigate clinicopathologic significance of OTOR in breast infiltrating ductal carcinoma (IDC) with high metastasis and unveil its biological function in BC. OTOR was highly overexpressed in BC tissues and cells compared with normal samples. OTOR overexpression was associated with a few of clinicopathologic characteristics and worse overall survival (OS) of IDC breast patients. Silencing of OTOR using siRNA tool impeded BC cells proliferation, migration and invasion by CCK-8, colony formation, wound-healing and transwell assays, which might be regulated by mitogen-activated protein kinase/extracellula...
Source: Biochemistry and Cell Biology - March 21, 2019 Category: Biochemistry Authors: Wang YF, Han J Tags: Biochem Cell Biol Source Type: research

Cationic lipoplexes for treatment of cancer stem cell-derived murine lung tumors
This study outlines the fabrication of a lipoplex of SOX2 small interfering RNA (CL-siSOX2) for targeted treatment of SOX2-enriched, CSC-derived orthotopic and xenograft lung tumors in CB-17 SCID mice. CL-siSOX2 induced tumor contraction in cisplatin-naïve and cisplatin-treated groups by 85% and 94% respectively. Reduction in tumor weight and volume following treatment with CL-siSOX2 was associated with reduced protein expression of SOX2 and markers of tumor initiation, inflammation, invasion and metastasis in mice tumor xenografts. In addition, histological staining of lung tumor sections showed reduction in SOX2 express...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 20, 2019 Category: Nanotechnology Source Type: research

Activation of Nrf2 signaling by Icariin protects against 6 ‐OHDA‐induced neurotoxicity
This study aimed at analyzing whether ICA evoked neuroprotection against 6‐hydroxydopamine (6‐OHDA)‐induced neu rotoxicity in PC12 cells and the mechanisms of action. ICA protected against 6‐OHDA‐induced neuronal damage, accompanied by the inhibition of cell apoptosis through the marked decreases in the Bax/Bcl‐2 ratio, cytochrome C release and caspase‐3 cleavage. In addition, the activation of Nrf2 s ignaling pathway was responsible for ICA‐mediated neuroprotection. First, ICA relieved reactive oxygen species (ROS) accumulation and increased superoxide dismutase (SOD) generation via the activation of Nrf2 ...
Source: Biotechnology and Applied Biochemistry - March 20, 2019 Category: Biochemistry Authors: Li Zhu, DaiDi Li, Ce Chen, GuoQing Wang, JingShan Shi, Feng Zhang Tags: Original Article Source Type: research

Identification of transmembrane protein 237 as a novel interactor with the intestinal riboflavin transporter-3 (RFVT-3): role in functionality and cell biology.
Abstract The apically localized riboflavin (RF) transporter-3 (RFVT-3) is involved in intestinal absorption of vitamin B2. Previous studies have characterized different physiological/biological aspects of the RFVT-3, but there is a lack of knowledge regarding possible existence of interacting partner(s) and consequence of interaction (s) on its function/cell biology. To address the latter, we performed yeast two-hybrid (Y2H) screening of a human colonic cDNA library and have identified transmembrane protein 237 (TMEM237) as a putative interactor with the human (h)RFVT-3; the interaction was further confirmed via "...
Source: Am J Physiol Cell Ph... - March 20, 2019 Category: Cytology Authors: Sabui S, Subramanian VS, Pham Q, Said HM Tags: Am J Physiol Cell Physiol Source Type: research

Intestinal Epithelial Tight Junction Barrier regulation by Autophagy related protein ATG6/beclin 1.
Abstract Defective tight junction (TJ) barrier is a key pathogenic factor for Inflammatory Bowel Disease. Previously, we have shown that autophagy, a cell survival mechanism, enhances intestinal epithelial TJ barrier function. Autophagy related protein ATG6/beclin 1, a key protein in the autophagy pathway, also plays a role in the endocytic pathway. The constitutive role of beclin 1 in intestinal TJ barrier is not known. In Caco-2 cells, beclin 1 was found to be co-immunoprecipitated with the TJ protein occludin and co-localized with occludin on the membrane. Treatment of Caco-2 cells with beclin 1 peptide (Tat-be...
Source: Am J Physiol Cell Ph... - March 20, 2019 Category: Cytology Authors: Wong M, Ganapathy AS, Suchanec E, Laidler L, Ma T, Nighot PK Tags: Am J Physiol Cell Physiol Source Type: research

Nuclear localization of Newcastle disease virus matrix protein promotes virus replication by affecting viral RNA synthesis and transcription and inhibiting host cell transcription
In this study, the potential functions of the M protein in the nucleus were investigated. We first demonstrate that nuclear localization of the M protein could not only promote the cytopathogenicity of NDV but also increase viral RNA synthesis and transcription efficiency in DF-1 cells. Using microarray analysis, we found that nuclear localization of the M protein might inhibit host cell transcription, represented by numerous up-regulating genes associated with transcriptional repressor activity and down-regulating genes associated with transcriptional activator activity. The role of representative up-regulated gene prospe...
Source: Veterinary Research - March 20, 2019 Category: Veterinary Research Source Type: research

Activation of Nrf2 signaling by Icariin protects against 6 ‐OHDA‐induced neurotoxicity
This study aimed at analyzing whether ICA evoked neuroprotection against 6‐hydroxydopamine (6‐OHDA)‐induced neu rotoxicity in PC12 cells and the mechanisms of action. ICA protected against 6‐OHDA‐induced neuronal damage, accompanied by the inhibition of cell apoptosis through the marked decreases in the Bax/Bcl‐2 ratio, cytochrome C release and caspase‐3 cleavage. In addition, the activation of Nrf2 s ignaling pathway was responsible for ICA‐mediated neuroprotection. First, ICA relieved reactive oxygen species (ROS) accumulation and increased superoxide dismutase (SOD) generation via the activation of Nrf2 ...
Source: Biotechnology and Applied Biochemistry - March 20, 2019 Category: Biochemistry Authors: Li Zhu, DaiDi Li, Ce Chen, GuoQing Wang, JingShan Shi, Feng Zhang Tags: Original Article Source Type: research

The oncogenic role of Wnt10a in colorectal cancer through activation of canonical Wnt/ β-catenin signaling.
In conclusion, the present study demonstrated that Wnt10a may have an oncogenic role during carcinogenesis of CRC through activation of Wnt/β-catenin signaling. PMID: 30881490 [PubMed]
Source: Oncology Letters - March 19, 2019 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Cancers, Vol. 11, Pages 386: Radiosensitivity of Cancer Cells Is Regulated by Translationally Controlled Tumor Protein
This study reveals that the regulation of radiosensitivity of cancer cells is yet another function of TCTP. The relationship between endogenous TCTP levels and sensitivity to radiation was examined in breast cancer cell lines (T47D, MDA-MB-231, and MCF7) and lung cancer cells lines (A549, H1299, and H460). Cancer cells with high expression levels of TCTP were more resistant to radiation. TCTP overexpression inhibited radiation-induced cell death, while silencing TCTP led to an increase in radiosensitivity. DNA damage in the irradiated TCTP-silenced A549 cells was greater than in irradiated control shRNA-transfected A549 ce...
Source: Cancers - March 19, 2019 Category: Cancer & Oncology Authors: Jiwon Jung Ji-Sun Lee Yun-Sil Lee Kyunglim Lee Tags: Article Source Type: research

TRPM2 mediates distruption of autophagy machinery and correlates with the grade level in prostate cancer
ConclusionWe showed that the autophagy –TRPM2 association may take place in the molecular basis of PCa and accordingly this connection may be targeted as a new therapeutic approach in PCa.
Source: Journal of Cancer Research and Clinical Oncology - March 19, 2019 Category: Cancer & Oncology Source Type: research

FER1L4/miR-372/E2F1 works as a ceRNA system to regulate the proliferation and cell cycle of glioma cells.
In this study, it was found that the expression of LncRNA FER1L4 is upregulated in high-grade gliomas than in low-grade cases and that a high expression of LncRNA FER1L4 predicts poor prognosis of gliomas. Meanwhile, in vitro study suggests that expression of FER1L4 with SiRNA knockdown obviously suppresses cell cycle and proliferation. It is further demonstrated by experiments that the FER1L4 knockdown suppresses growth of in vivo glioma. Besides, it is found in our study that LncRNA FER1L4 expression is positively correlated with E2F1 mRNA expression. After knockdown of FER1L4 expression, E2F1 expression is significantly...
Source: J Cell Mol Med - March 19, 2019 Category: Molecular Biology Authors: Xia L, Nie D, Wang G, Sun C, Chen G Tags: J Cell Mol Med Source Type: research

GSE118101 Next Generation Sequencing upon siRNA-mediated knockdown of DRAIC in MCF-7 cells.
Contributor : Imke TiessenSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe goal of this study is to examine the effect of DRAIC knockdown on the MCF-7 transcriptome.
Source: GEO: Gene Expression Omnibus - March 18, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Identifying CYR61 as a Potential Anti ‐fibrotic and Pro‐angiogenic Mediator in Scleroderma
ConclusionsWe identified CYR61, which is epigenetically regulated by HDAC5, as a potent anti ‐fibrotic and pro‐angiogenic mediator in SSc. Therapeutic intervention to promote CYR61 activity or increase CYR61 levels might be of potential benefit in SSc.This article is protected by copyright. All rights reserved.
Source: Arthritis and Rheumatology - March 18, 2019 Category: Rheumatology Authors: Pei ‐Suen Tsou, Dinesh Khanna, Amr H. Sawalha Tags: Full Length Source Type: research

Viruses, Vol. 11, Pages 271: Mosquito Small RNA Responses to West Nile and Insect-Specific Virus Infections in Aedes and Culex Mosquito Cells
P. Pijlman Small RNA mediated responses are essential for antiviral defence in mosquitoes, however, they appear to differ per virus-vector combination. To further investigate the diversity of small RNA responses against viruses in mosquitoes, we applied a small RNA deep sequencing approach on five mosquito cell lines: Culex tarsalis CT cells, Aedes albopictus U4.4 and C6/36 cells, Ae. aegypti Aag2 cells (cleared from cell fusing agent virus and Culex Y virus (CYV) by repetitive dsRNA transfections) and Ae. pseudoscutellaris AP-61 cells. De novo assembly of small RNAs revealed the presence of Phasi Charoen-like virus (P...
Source: Viruses - March 18, 2019 Category: Virology Authors: Giel P. G öertz Pascal Miesen Gijs J. Overheul Ronald P. van Rij Monique M. van Oers Gorben P. Pijlman Tags: Article Source Type: research

Endo-siRNAs regulate early embryonic development by inhibiting transcription of long terminal repeat sequence in pig.
Abstract Activity of some endogenous retroviruses (ERVs) has been proven to be important for development of early mammalian embryo. However, abnormal activation of ERVs can also cause genetic diseases due to their ability to retrotranspose, so the regulatory mechanism to limit transcription of ERVs needs to be clarified. Endogenous small interfering RNA (endo-siRNA) has been reported to protect cells against transposable elements (TEs). Here, we determined the role of ERVs long terminal repeat sequences (LTRs) derived endo-siRNAs (LTR-siRNAs) on inhibition of the activity of ERVs during early embryonic development...
Source: Biology of Reproduction - March 18, 2019 Category: Reproduction Medicine Authors: Kong Q, Quan X, Du J, Tai Y, Liu W, Zhang J, Zhang X, Mu Y, Liu Z Tags: Biol Reprod Source Type: research

Inheritance of a Phenotypically Neutral Epimutation Evokes Gene Silencing in Later Generations
Duempelmann et  al. describe an H3K9me3 and siRNA-dependent epimutation, which is installed during gene silencing and inherited across generations, even upon reactivation of the silenced gene. Yet silencing is reinstated in later generations if Paf1C is impaired, revealing H3K9me3 as a stable epigenetic mark that is not repressive per se.
Source: Molecular Cell - March 18, 2019 Category: Cytology Authors: Lea Duempelmann, Fabio Mohn, Yukiko Shimada, Daniele Oberti, Aude Andriollo, Silke Lochs, Marc B ühler Tags: Article Source Type: research

Characterization of In vitro inhibitory effects of consensus short interference RNAs against non-structural 5B gene of hepatitis C virus 1a genotype
Conclusions: Stable Huh-7 cells persistently expressing NS5B protein should be helpful for molecular pathogenesis of HCV infection and development of anti-HCV drug screening assays. The siRNA was effective against NS5B and could be considered as an adjuvant therapy along with other promising anti-HCV regimens.
Source: Indian Journal of Medical Microbiology - March 18, 2019 Category: Microbiology Authors: Imran Shahid Waleed Hassan Almalki Munjed M Ibrahim Sultan Ahmad Alghamdi Mohammed H Mukhtar Shaia Saleh R. Almalki Saad Ahmed Alkahtani Mohammad S Alhaidari Source Type: research

Cytoprotective effects of euxanthone against ox-LDL-induced endothelial cell injury is mediated via Nrf2
Publication date: Available online 16 March 2019Source: Life SciencesAuthor(s): Shengnan Li, Yong Sun, Zhiwu Han, Xiaocui Bu, Weijie Yu, Junyu WangAbstractAimAtherosclerosis (AS) is a chronic condition of the arterial vessels and a risk factor for myocardial infarction and stroke. Euxanthone is a xanthone compound extracted from Polygala caudata, and shows vasodilatory action. The aim of this study was to determine the potential pharmacological effects of euxanthone against oxidized low-density lipoprotein (ox-LDL)-induced endothelial cell injury.Material and methodsHuman umbilical vein endothelial cells (HUVECs) were expo...
Source: Life Sciences - March 17, 2019 Category: Biology Source Type: research

Abdominal Aortic Transplantation of Bone Marrow Mesenchymal Stem Cells Regulates the Expression of Ciliary Neurotrophic Factor and Inflammatory Cytokines in a Rat Model of Spinal Cord Ischemia-Reperfusion Injury.
CONCLUSIONS In a rat model of spinal cord ischemia-reperfusion injury, abdominal aortic transplantation of BMMSCs increased the expression of CNTF, which improved hindlimb locomotor recovery by regulating the expression of IL-6 and IL-10 to reduce inflammation of the spinal cord. PMID: 30875363 [PubMed - in process]
Source: Medical Science Monitor - March 16, 2019 Category: Research Tags: Med Sci Monit Source Type: research

Long non-coding RNA CRNDE regulates cell proliferation, migration, invasion, epithelial-mesenchymal transition and apoptosis in oral squamous cell carcinoma.
In conclusion, silencing CRNDE may inhibit EMT, thus decreasing the migration and invasion of human OSCC cells by repressing the activation of the Wnt/β-catenin signaling pathway, thereby restricting cell growth and promoting cell apoptosis. PMID: 30867767 [PubMed]
Source: Oncology Letters - March 16, 2019 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

GSE118679 CELSeq2-profiles for siRNA screening and daily timecourse for early reprogramming to pluripotency.
Contributors : Georgina Pe ñalosa Ruiz ; Jan P Gerlach ; Gert J Veenstra ; Klaas MulderSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusAIM: To find molecular signatures associated to the siRNA-mediated knockdowns in order to be able to identify similarities among different knockdowns.DESCRIPTION: Each sample includes biological triplicates for 35 siRNA-mediated knockdowns targeting 30 chromatin-associated proteins during in early reprogramming to iPS at day 6. A daily timecourse from reprogramming cells, without treatment from MEFs until day 6 is also included in triplicate.
Source: GEO: Gene Expression Omnibus - March 14, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

Carbosilane dendrimers with phosphonium terminal groups are low toxic non-viral transfection vectors for siRNA cell delivery
Publication date: Available online 13 March 2019Source: International Journal of PharmaceuticsAuthor(s): Regina Herma, Dominika Wrobel, Michaela Liegertová, Monika Müllerová, Tomáš Strašák, Marek Maly, Alena Semerádtová, Marcel Štofik, Dietmar Appelhans, Jan MalyAbstractNon-viral gene delivery vectors studied in the gene therapy applications are often designed with the cationic nitrogen containing groups necessary for binding and cell release of nucleic acids. Disadvantage is a relatively high toxicity which restricts the in vivo use of such nanoparticles. Here we show, that the 3rd generation carbosilane dendrim...
Source: International Journal of Pharmaceutics - March 13, 2019 Category: Drugs & Pharmacology Source Type: research

Cytoplasmic Delivery of Functional siRNA Using pH-Responsive Nanoscale Hydrogels
Publication date: Available online 8 March 2019Source: International Journal of PharmaceuticsAuthor(s): William B. Liechty, Rebekah L. Scheuerle, Julia E. Vela Ramirez, Nicholas A. PeppasAbstractThe progress of short interfering RNA (siRNA) technologies has unlocked the development of novel alternatives for the treatment of a myriad of diseases, including viral infections, autoimmune disorders, or cancer. Nevertheless, the clinical use of these therapies faces significant challenges, mainly overcoming the charged and large nature of these molecules to effectively enter the cell. In this work, we developed a cationic polyme...
Source: International Journal of Pharmaceutics - March 9, 2019 Category: Drugs & Pharmacology Source Type: research

----Delivery of siRNA therapeutics: PLGA nanoparticles approach.
Authors: Matta J, Maalouf R Abstract RNA interference is currently one of the most advanced genomic tools providing promising insight to manage countless pathophysiological conditions that seemed unapproachable few years ago. Researchers across the globe have devised numerous methods in which small RNA molecules can be administered into target cells to manipulate cellular functions at the molecular level and eradicate defects that lead to pronounced phenotypes, which might sometimes be as fatal as malignant tumors. The present review provides an overview of the non-viral delivery approaches for siRNA therapeutics w...
Source: Frontiers in Bioscience - Scholar - March 9, 2019 Category: Biomedical Science Tags: Front Biosci (Schol Ed) Source Type: research

Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery.
Abstract Pulmonary siRNA delivery has attracted strong interest and has been reported to successfully mediate target gene knockdown in a number of disease models. However, the nature of the epithelial cells that eventually take up siRNA and the question if other lung cell types may also be transfected has so far been neglected. Therefore, we describe here a flow cytometry-based method using transgenic enhanced green fluorescence protein (EGFP) expressing mice for the differentiation of transfected lung cell populations based on their antigen expression. PMID: 30838629 [PubMed - in process]
Source: Mol Biol Cell - March 8, 2019 Category: Molecular Biology Authors: Merkel OM, Marsh LM, Garn H, Kissel T Tags: Methods Mol Biol Source Type: research

Evaluating the Regulation of Cytokine Levels After siRNA Treatment in Antigen-Specific Target Cell Populations via Intracellular Staining.
Abstract RNA interference (RNAi) offers a promising base for therapeutic knockdown of clinically relevant genes. Local delivery routes as well as targeted delivery to specific cell populations have been shown to circumvent several hurdles of successful siRNA delivery in vivo. To evaluate and quantify the treatment effect in a precise way, next to measuring the downregulation on gene and protein levels, it is equally essential to investigate the influence on downstream factors such as generated cytokines. Here, we describe an expressive method to specifically isolate the desired target cells and determine their lev...
Source: Mol Biol Cell - March 8, 2019 Category: Molecular Biology Authors: Kandil R, Feldmann D, Xie Y, Merkel OM Tags: Methods Mol Biol Source Type: research

Preparation, Characterization, and In Vitro Evaluation of Lipidoid-Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol.
Abstract RNA interference (RNAi) therapeutics are one of the most promising biological interventions in the efficient management of difficult-to-treat diseases. RNAi is mediated by small interfering RNA (siRNA), which induces specific and highly potent gene silencing. However, intracellular delivery of exogenous, chemically synthesized siRNA to the RNAi pathway in the cytosol remains a challenge, and is fully dependent on technologies that can facilitate cytosolic delivery without undesired side effects. One example is a novel delivery system referred to as lipidoid-polymer hybrid nanoparticles (LPNs), which we re...
Source: Mol Biol Cell - March 8, 2019 Category: Molecular Biology Authors: Thanki K, Zeng X, Foged C Tags: Methods Mol Biol Source Type: research

Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery.
Abstract Polymerized micelles obtained by photopolymerization of diacetylenic surfactants and which are forming polydiacetylenic systems (PDAs) have recently gained interest as stabilized monodisperse systems showing potential for the delivery of hydrophobic drugs as well as of larger biomolecules such as nucleic acids. Introduction of pH-sensitive histidine groups at the surface of the micellar PDA systems allows for efficient delivery of siRNA resulting in specific gene silencing through RNA interference. Here, we describe the detailed experimental procedure for the reproducible preparation of these photopolymer...
Source: Mol Biol Cell - March 8, 2019 Category: Molecular Biology Authors: Ripoll M, Neuberg P, Remy JS, Kichler A Tags: Methods Mol Biol Source Type: research

Histidine-Rich Cationic Cell-Penetrating Peptides for Plasmid DNA and siRNA Delivery.
Abstract Amphipathic, pH-responsive, membrane-active peptides such as LAH4 and derivatives thereof have the ability to effectively deliver genes and small interfering RNA (siRNA) into mammalian cells. Their ability to bind and protect nucleic acids and then disrupt membranes when activated at low pH enables them to harness the endocytic machinery to deliver cargo efficiently and with low associated toxicity. This chapter describes protocols for the chemical synthesis of transfection peptides of the LAH4 family, complex formation with nucleic acids, and their use for the in vitro delivery of either plasmid DNA or s...
Source: Mol Biol Cell - March 8, 2019 Category: Molecular Biology Authors: Kichler A, Mason AJ, Marquette A, Bechinger B Tags: Methods Mol Biol Source Type: research

Cancers, Vol. 11, Pages 333: Therapeutic Targeting of Stat3 Using Lipopolyplex Nanoparticle-Formulated siRNA in a Syngeneic Orthotopic Mouse Glioma Model
r Donat Kögel Glioblastoma (GBM), WHO grade IV, is the most aggressive primary brain tumor in adults. The median survival time using standard therapy is only 12–15 months with a 5-year survival rate of around 5%. Thus, new and effective treatment modalities are of significant importance. Signal transducer and activator of transcription 3 (Stat3) is a key signaling protein driving major hallmarks of cancer and represents a promising target for the development of targeted glioblastoma therapies. Here we present data showing that the therapeutic application of siRNAs, formulated in nanoscale lipopolyplexe...
Source: Cancers - March 8, 2019 Category: Cancer & Oncology Authors: Benedikt Linder Ulrike Weirauch Alexander Ewe Anja Uhmann Volker Seifert Michel Mittelbronn Patrick N. Harter Achim Aigner Donat K ögel Tags: Article Source Type: research

Self-assembled lipid nanoparticles for ratiometric codelivery of cisplatin and siRNA targeting XPF to combat drug resistance in lung cancer.
Abstract DNA damage repair through nucleotide excision repair (NER) pathway is one of the major reasons for the decreased antitumor efficacy of platinum-based anticancer drugs that have been widely applied in the clinic. Inhibiting intrinsic NER function may enhance the antitumor activity of cisplatin and conquer cisplatin resistance. Herein, we report the design, optimization, and application of a self-assembled lipid nanoparticle (LNP) system to simultaneously deliver a cisplatin prodrug together with siRNA targeting endonuclease XPF, a crucial component in the NER pathway. The LNP is able to efficiently encapsu...
Source: Chemistry, an Asian Journal - March 6, 2019 Category: Chemistry Authors: Li C, Li T, Huang L, Yang M, Zhu G Tags: Chem Asian J Source Type: research

DNA Tetrahedron-Based Nanogels for siRNA Delivery and Gene Silencing
Chem. Commun., 2019, Accepted Manuscript DOI: 10.1039/C9CC00175A, CommunicationHan Xue, Fei Ding, Jiao Zhang, Yuanyuan Guo, Xihui Gao, Jing Feng, Xinyuan Zhu, Chuan Zhang DNA tetrahedrons are employed as building blocks to construct a novel DNA-based nanogel for intracellular siRNA delivery. With compact architecture that embeds functional siRNAs inside, the self-assembled nanogel can efficiently... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 1, 2019 Category: Chemistry Authors: Han Xue Source Type: research

Graphene oxide functionalized with chitosan based nanoparticles as a carrier of siRNA in regulating Bcl-2 expression on Saos-2 & MG-63 cancer cells and its inflammatory response on bone marrow derived cells from mice
Publication date: June 2019Source: Materials Science and Engineering: C, Volume 99Author(s): Shanmuga Sundar Saravanabhavan, Muruganantham Rethinasabapathy, Sarang Zsolt, Aravind Bhat Kalambettu, Sundaravadivel Elumalai, Manokaran Janakiraman, Yun Suk Huh, Balasubramanian NatesanAbstractPresently, quite a lot of research that are being carried out to find a potential cure for cancer and many had made to clinical trial stage as well. In the present study, we focus on use of a novel graphene oxide functionalized chitosan nanoparticle targeting Saos-2 and MG-63 osteosarcoma cells. The graphene oxide chitosan nanoparticles wer...
Source: Materials Science and Engineering: C - March 1, 2019 Category: Materials Science Source Type: research

Intravitreal Ets1 siRNA alleviates choroidal neovascularization in a mouse model of age-related macular degeneration
In this study, Ets1 increased and localized in the macrophages and microglia of a mouse laser-induced CNV region. Ets1 siRNA intravitreal injection ameliorated the leakage and area of CNV, as well as inhibiting the dysfunction of retinal pigment epithelium (RPE) cells and the activation of macrophages/microglia. Taken together, we provide a new insight into the molecular mechanism of CNV progression, in which Ets1 can be a new therapeutic target.
Source: Cell and Tissue Research - March 1, 2019 Category: Cytology Source Type: research

Delivery of BACE1 siRNA mediated by TARBP-BTP fusion protein reduces β-amyloid deposits in a transgenic mouse model of Alzheimer's disease.
In this report, we further substantiate the approach through an extended use in AβPP-PS1 mice, which upon treatment with seven doses of β-secretase AβPP cleaving Enzyme 1 (BACE1) TARBP-BTP:siRNA, led to target-specific effect in the mouse brain. Concomitant gene silencing of BACE1, and consequent reduction in plaque load in the cerebral cortex and hippocampus (greater than 60%) in mice treated with TARBP-BTP:siRNA complex, led to improvement in spatial learning and memory. The study validates the efficiency of TARBP-BTP fusion protein as an efficient mediator of RNAi, giving considerable scope for future intervention in...
Source: Journal of Biosciences - March 1, 2019 Category: Biomedical Science Authors: Haroon MM, Saba K, Boddedda VH, Kumar JM, Patel AB, Gopal V Tags: J Biosci Source Type: research

CD44 targeted delivery of siRNA by using HA-decorated nanotechnologies for KRAS silencing in cancer treatment
In this study we developed ternary nanocomplexes to deliver an anti-KRAS siRNA to colorectal cancer cells, exploiting the interaction of hyaluronic acid (HA) with CD44 as a means to achieve selective targeting of CD44-positive cancer cells. Two different polycations, poly(hexamethylene biguanide) and chitosan, were complexed with siRNA and coated with HA. Physico-chemical properties and stability of nanoparticles were characterized, including size, surface charge, and degree of siRNA protection. We demonstrate nanoparticle internalization (flow cytometry), siRNA cytosolic release (confocal microscopy) and KRAS silencing (R...
Source: International Journal of Pharmaceutics - February 27, 2019 Category: Drugs & Pharmacology Source Type: research

Versatility of cell-penetrating peptides for intracellular delivery of siRNA.
Authors: Singh T, Murthy ASN, Yang HJ, Im J Abstract The plasma membrane is a large barrier to systemic drug delivery into cells, and it limits the efficacy of drug cargo. This issue has been overcome using cell-penetrating peptides (CPPs). CPPs are short peptides (6-30 amino acid residues) that are potentially capable of intracellular penetration to deliver drug molecules. CPPs broadened biomedical applications and provide a means to deliver a range of biologically active molecules, such as small molecules, proteins, imaging agents, and pharmaceutical nanocarriers, across the plasma membrane with high efficacy and...
Source: Drug Delivery - February 27, 2019 Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research

Magnetically responsive hybrid nanoparticles for in vitro siRNA delivery to breast cancer cells
Publication date: June 2019Source: Materials Science and Engineering: C, Volume 99Author(s): Milene Dalmina, Frederico Pittella, Jelver Alexander Sierra, Gabriela Regina Rosa Souza, Adny Henrique Silva, André Avelino Pasa, Tânia Beatriz Creczynski-PasaAbstractShort interfering RNA (siRNA) showed to be a viable alternative to a better prognosis in cancer therapy. Nevertheless, the successful application of this strategy still depends on the development of nanocarriers for the safe delivery of siRNA into the diseased tissue, which mostly occurs by passive accumulation. When an external magnetic field is applied, magnetic n...
Source: Materials Science and Engineering: C - February 23, 2019 Category: Materials Science Source Type: research