Query: siRNA

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Total 17216 results found since Jan 2013.

miR-126 Suppresses Invasion and Migration of Malignant Glioma by Targeting Mature T Cell Proliferation 1 (MTCP1).
CONCLUSIONS miR-126 inhibits the migration of malignant glioma cells by inhibiting MTCP1. PMID: 30233082 [PubMed - in process]
Source: Medical Science Monitor - September 22, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Dependence of HSP27 cellular level on protein kinase CK2 discloses novel therapeutic strategies
ConclusionBeing HSP27 involved in several pathological conditions, including protein conformational diseases (i.e Cystic Fibrosis) and cancer, the need of drugs to modulate its activity is growing and CK2-targeting could represent a new strategy to reduce cellular HSP27 level.General significanceThis study identifies CK2 as a molecular target to control HSP27 cellular expression.
Source: Biochimica et Biophysica Acta (BBA) General Subjects - September 22, 2018 Category: Biochemistry Source Type: research

S100P and Ezrin promote trans-endothelial migration of triple negative breast cancer cells
ConclusionsFrom our data we conclude that S100P, Ezrin and EzrinThr-567 are involved in the trans-endothelial migration of TNBC cells and that they may serve as potential targets in TNBC patients.
Source: Cellular Oncology - September 22, 2018 Category: Cancer & Oncology Source Type: research

Transcription factor 7-like 2 controls matrix degradation through nuclear factor κB signaling and is repressed by microRNA-155 in nucleus pulposus cells
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Jianchao Sun, Junmin Hong, Siguo Sun, Xiaofei Wang, Yan Peng, Jie Zhou, Yingjie Huang, Shuangxing Li, Weijian Chen, Chunhai Li, Kang Xu, Wei YeAbstractAimTCF7L2, a key transcription factor in the canonical Wnt pathway, plays a vital role in the matrix degradation of chondrocytes. However, it is unknown whether TCF7L2 is important in the matrix metabolism of inner gel-like nucleus pulposus (NP) cells; thus, the aim of this study was to explore the effect and mechanism of TCF7L2 in this process.MethodsWestern blotting and immunofluores...
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Silencing Bmi1 expression suppresses cancer stemness and enhances chemosensitivity in endometrial cancer cells
ConclusionBmi1 silencing suppresses cancer stemness in HEC1A and Ishikawa cells. Concurrent treatment with Bmi1 siRNA and cisplatin resulted in additive anticancer effect with a cell line-specific pattern, which was higher than that shown by cisplatin treatment alone.Graphical abstract
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Downregulated caveolin-1 expression serves a potential role in coronary artery spasm by inducing nitric oxide production in vitro.
In conclusion, the downregulated Cav-1 expression served a key role in NO production in the in vitro model of CAS induced by ACh stimulation of LPS-damaged HUVECs. PMID: 30233709 [PubMed]
Source: Experimental and Therapeutic Medicine - September 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Thymosin β4 promotes glucose-impaired endothelial progenitor cell function via Akt/endothelial nitric oxide synthesis signaling pathway.
Authors: Qiu F, Song J, Bi X, Wang M, Zhao Y, Fu G Abstract Circulating endothelial progenitor cells (EPCs) are a subtype of hematopoietic stem cells, which can differentiate into endothelial cells and restore endothelial function. However, high glucose decreases the number and impairs the function of EPCs. A previous study showed that thymosin β4 (Tβ4), a pleiotropic peptide beneficial for multiple functions of various types of cells, could promote EPC migration and dose-dependently upregulate the phosphorylation of Akt and endothelial nitric oxide synthesis signaling (eNOS). In present study, the hypothesis tha...
Source: Experimental and Therapeutic Medicine - September 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Desmoglein-2 overexpression predicts poor prognosis in hepatocellular carcinoma patients.
CONCLUSIONS: Taken together, our results suggest that increased Dsg2 expression was associated with tumor progression in HCC and may function as a promising biomarker for unfavorable prognosis of HCC. PMID: 30229819 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - September 21, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

High-content analysis for mitophagy response to nanoparticles: A potential sensitive biomarker for nanosafety assessment
Publication date: Available online 20 September 2018Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Chengyong He, Shengwei Jiang, Huan Yao, Liyin Zhang, Chuanli Yang, Shan Jiang, Fengkai Ruan, Denglin Zhan, Gang Liu, Zhongning Lin, Yuchun Lin, Xiaoyuan ChenAbstractMitophagy, a selective autophagy of mitochondria, clears up damaged mitochondria to maintain cell homeostasis. We performed high-content analysis (HCA) to detect the increase of PINK1, an essential protein controlling mitophagy, in hepatic cells treated with several nanoparticles (NPs). PINK1 immunofluorescence-based HCA was more sensitive th...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - September 21, 2018 Category: Nanotechnology Source Type: research

Parkin Modulates ERR α/eNOS Signaling Pathway in Endothelial Cells
Conclusion: These findings suggested that parkin overexpression downregulated eNOS possibly through the ubiquitination of ERR α in endothelial cells.Cell Physiol Biochem 2018;49:2022 –2034
Source: Cellular Physiology and Biochemistry - September 21, 2018 Category: Cytology Source Type: research

Cancers, Vol. 10, Pages 347: NACC1, as a Target of MicroRNA-331-3p, Regulates Cell Proliferation in Urothelial Carcinoma Cells
Fujimoto Chiho Ohbayashi The nucleus accumbens-associated protein 1 (NACC1) is a transcription factor constitutively expressed in the urothelium, where it regulates cell growth, senescence, autophagy, and epithelial-mesenchymal transition. microRNA (miRNA) constitutes a class of small non-coding RNAs which are involved in cell proliferation, differentiation, and progression of tumors. miRNAs and their target molecules are utilized for molecular diagnosis of urothelial carcinoma. NACC1 is one of several putative target molecules of miR-331-3p, and is associated with cell proliferation in cancers such as prostate and ...
Source: Cancers - September 21, 2018 Category: Cancer & Oncology Authors: Kohei Morita Tomomi Fujii Hiroe Itami Tomoko Uchiyama Tokiko Nakai Kinta Hatakeyama Aya Sugimoto Makito Miyake Yasushi Nakai Nobumichi Tanaka Keiji Shimada Masaharu Yamazaki Kiyohide Fujimoto Chiho Ohbayashi Tags: Article Source Type: research

TNF-{alpha} elicits phenotypic and functional alterations of vascular smooth muscle cells by miR-155-5p-dependent down-regulation of cGMP-dependent kinase 1 Cell Biology
cGMP-dependent protein kinase 1 (PKG1) plays an important role in nitric oxide (NO)/cGMP–mediated maintenance of vascular smooth muscle cell (VSMC) phenotype and vasorelaxation. Inflammatory cytokines, including tumor necrosis factor-α (TNFα), have long been understood to mediate several inflammatory vascular diseases. However, the underlying mechanism of TNFα-dependent inflammatory vascular disease is unclear. Here, we found that TNFα treatment decreased PKG1 expression in cultured VSMCs, which correlated with NF-κB–dependent biogenesis of miR-155-5p that targeted the 3′-UTR of PKG1 mRNA. TNFα induced VSMC phe...
Source: Journal of Biological Chemistry - September 21, 2018 Category: Chemistry Authors: Seunghwan Choi, Minsik Park, Joohwan Kim, Wonjin Park, Suji Kim, Dong-Keon Lee, Jong Yun Hwang, Jongseon Choe, Moo-Ho Won, Sungwoo Ryoo, Kwon-Soo Ha, Young-Guen Kwon, Young-Myeong Kim Tags: Cell Biology Source Type: research

Caged siRNAs with single folic acid modification of antisense RNA for photomodulation of exogenous and endogenous gene expression in cells
Org. Biomol. Chem., 2018, Advance Article DOI: 10.1039/C8OB01952E, PaperLijia Yu, Nannan Jing, Zhenjun Yang, Lihe Zhang, Xinjing Tang Photoregulating gene expression using folic acid modified caged siRNA through complex formation of folic acid/folate receptor. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic and Biomolecular Chemistry - September 20, 2018 Category: Molecular Biology Authors: Lijia Yu Source Type: research

Inhibition of microRNA-30d attenuates the apoptosis and extracellular matrix degradation of degenerative human nucleus pulposus cells by up-regulating SOX9
In this study, we aimed to investigate the role of miR-30d in regulating apoptosis and the extracellular matrix degradation of NP cells, along with the potential underlying molecular mechanism. Herein, our results showed that miR-30d was significantly increased in degenerative NP tissues compared with normal controls. Functional experiments showed that the inhibition of miR-30d promoted the viability and reduced the apoptosis of NP cells in vitro. Moreover, miR-30d inhibition increased the expression of type II collagen and aggrecan and inhibited the expression of matrix metalloproteinase. In contrast, the overexpression o...
Source: Chemico Biological Interactions - September 20, 2018 Category: Biochemistry Source Type: research

Biological functions and clinical significance of the newly identified long non ‑coding RNA RP1‑85F18.6 in colorectal cancer.
Biological functions and clinical significance of the newly identified long non‑coding RNA RP1‑85F18.6 in colorectal cancer. Oncol Rep. 2018 Sep 10;: Authors: Ma Y, Chen Y, Lin C, Hu G Abstract The biological functions of long non‑coding RNAs (lncRNAs) in cancer have not been fully elucidated. The present study demonstrated that the expression of a newly identified lncRNA, RP1‑85F18.6, was upregulated in colorectal cancer (CRC) tissues and cell lines. Knockdown of lncRNA RP1‑85F18.6 served a key role in tumor inhibition, reduced cell proliferation and invasion, disrupted the cell cycle, and i...
Source: Oncology Reports - September 20, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Norepinephrine enhances cell viability and invasion, and inhibits apoptosis of pancreatic cancer cells in a Notch ‑1‑dependent manner.
In conclusion, NE may enhance the malignant biological behaviors of PDAC via activating the Notch‑1 pathway. PMID: 30226612 [PubMed - as supplied by publisher]
Source: Oncology Reports - September 20, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

STAT1 ‑HDAC4 signaling induces epithelial‑mesenchymal transition and sphere formation of cancer cells overexpressing the oncogene, CUG2.
STAT1‑HDAC4 signaling induces epithelial‑mesenchymal transition and sphere formation of cancer cells overexpressing the oncogene, CUG2. Oncol Rep. 2018 Sep 12;: Authors: Kaowinn S, Kaewpiboon C, Koh SS, Krämer OH, Chung YH Abstract Our previous studies have shown that the novel oncogene, cancer upregulated gene 2 (CUG2), activates STAT1, which is linked to anticancer drug resistance, induces epithelial‑mesenchymal transition (EMT) and cancer stem cell‑like phenotypes as determined by MTT, migration and sphere formation assays. We thus aimed to ascertain whether the activation of STAT1 by CUG2...
Source: Oncology Reports - September 20, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

LncRNA HOTTIP mediated DKK1 downregulation confers metastasis and invasion in colorectal cancer cells.
Authors: Rui Y, Hu M, Wang P, Zhang C, Xu H, Li Y, Zhang Y, Gu J, Wang Q Abstract Recent studies highlight long non-coding RNAs (lncRNAs) as key regulators of cancer biology that contribute to carcinogenesis. The lncRNA HOXA transcript at the distal tip (HOTTIP) is involved in the development of several cancers. Previous studies demonstrated that HOTTIP could promote colorectal cancer (CRC) cell proliferation via silencing of p21 expression. However, the potential role of HOTTIP in CRC metastasis has not yet been discussed. Here, we found that HOTTIP level was significantly higher in CRC than in corresponding adjac...
Source: Histology and Histopathology - September 20, 2018 Category: Cytology Tags: Histol Histopathol Source Type: research

Using Two-Fluid Nozzle for Spray Freeze Drying to Produce Porous Powder Formulation of Naked siRNA for Inhalation
In this study, we reported the use of two-fluid nozzle to produce spray freeze dried powder of small interfering RNA (siRNA). The effect of atomization gas flow rate and liquid feed rate were inspected initially using herring sperm DNA (hsDNA) as nucleic acid model. The atomization gas flow rate was found to have a major impact on the aerosol properties. The higher the atomization gas flow rate, the smaller the particle size, the higher the fine particle fraction (FPF). In contrast, the liquid feed rate had very minor effect. Subsequently, spray freeze dried siRNA powder was produced at various atomization gas flow rates. ...
Source: International Journal of Pharmaceutics - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

miR-191 Inhibition Induces Apoptosis Through Reactivating Secreted Frizzled-Related Protein-1 in Cholangiocarcinoma
Conclusion: Taken together, our results demonstrate a novel insight into miR-191 biological function in CCA. Our findings suggest that miR-191 is a potential therapeutic target of CCA treatment.Cell Physiol Biochem 2018;49:1933 –1942
Source: Cellular Physiology and Biochemistry - September 20, 2018 Category: Cytology Source Type: research

Genipin Reverses HFD-Induced Liver Damage and Inhibits UCP2-Mediated Pyroptosis in Mice
Conclusion: GNP reverses HFD-induced liver damage and inhibits UCP2-mediated pyroptosis. Thus, GNP may serve as a potential therapeutic candidate for NAFLD.Cell Physiol Biochem 2018;49:1885 –1897
Source: Cellular Physiology and Biochemistry - September 20, 2018 Category: Cytology Source Type: research

The downregulation of hsa_circ_0012919, sponge for miR-125a-3p, contributes to DNA methylation of CD11a and CD70 in CD4+T cells of SLE
Conclusion:Hsa_circ_0012919 could be regarded as a biomarker for SLE and hsa_circ_0012919 was the ceRNA for miR-125a-3p.
Source: Clinical Science - September 20, 2018 Category: Biomedical Science Authors: Zhang, C., Wang, X., Chen, Y., Wu, Z., Zhang, C., Shi, W. Tags: PublishAheadOfPrint Source Type: research

Label-free detection of protein tyrosine phosphatase 1B (PTP1B) using a rationally designed F örster resonance energy transfer (FRET) probe.
Label-free detection of protein tyrosine phosphatase 1B (PTP1B) using a rationally designed Förster resonance energy transfer (FRET) probe. Chembiochem. 2018 Sep 20;: Authors: Durgannavar T, Kwon SJ, Ghisaidoobe ABT, Rho K, Kim JH, Yoon SY, Kang HJ, Chung SJ Abstract A highly selective detection method of native protein tyrosine phosphatase 1B (PTP1B) is described using a target specific probe equipped with 1-naphthylamine (Ex = 330 nm, Em = 445 nm). Irradiation of a mixture of PTP1B and Probe 1 with ultraviolet light of 280 nm (correspon6ding to PTP1B excitation maximum) resulted in significant fluo...
Source: Chembiochem - September 20, 2018 Category: Biochemistry Authors: Durgannavar T, Kwon SJ, Ghisaidoobe ABT, Rho K, Kim JH, Yoon SY, Kang HJ, Chung SJ Tags: Chembiochem Source Type: research

Characterization and function of the T-box 1 gene in Chinese giant salamander Andrias davidianus.
Abstract We characterized the Andrias davidianus T-box 1 (Tbx1) gene. Tbx1 expression was high in testis and low in other examined tissues. Immunohistochemistry detected tbx1 expression in somatic and germ cells 62 days post-hatching (dph), prior to gonad differentiation. At 210 dph, after gonad differentiation, tbx1 was expressed in spermatogonia and testis somatic cells and in granulosa cells in ovary. Tbx1 expression was up-regulated in ovary after high temperature treatment. In the neomale, tbx1 expression showed a similar profile to normal males, and vice-versa for genetic male. Over-expression of tbx1 in f...
Source: Genomics - September 20, 2018 Category: Genetics & Stem Cells Authors: Hu Q, Meng Y, Wang D, Tian H, Xiao H Tags: Genomics Source Type: research

Apoptotic cell death induced by Z-Ligustilidein human ovarian cancer cells and role of NRF2.
Abstract Z-Ligustilide is the most potent bioactive component of Angelica sinensis, which is widely used in Chinese traditional medicine. Z-Ligustilide selectively affected ovarian cancer cell survival in a dose dependent manner. Z-Ligustilide induced apoptotic cell death was determined by flow cytometry. We also demonstrated that apoptotic cell death was triggered by Z-Ligustilideinduced oxidative stress and mitochondria played an active role. Mitochondrial polarization was reduced by Z-Ligustilidewhereas mitochondrial superoxide formation was increased. NRF2 was induced by Z-Ligustilide in OVCAR-3 cells at epi...
Source: Food and Chemical Toxicology - September 20, 2018 Category: Food Science Authors: Lang F, Qu J, Yin H, Li L, Zhi Y, Liu Y, Fang Z, Hao E Tags: Food Chem Toxicol Source Type: research

LncRNA HOTAIR mediates TGF- β2-induced cell growth and epithelial-mesenchymal transition in human lens epithelial cells.
In this study, we confirmed that TGF-β2 induces EMT in SRA01/04 cells via the up-regulation of the long non-coding RNA (lncRNA) HOTAIR. To study the effects of HOTAIR on the proliferation, migration and EMT of SRA01/04 cells as well as the underlying mechanism, we used small interfering RNA (siRNA) to specifically attenuate HOTAIR expression in SRA01/04 cells. CCK8 cell-counting kit was used to examine SRA01/04 cell viability; EdU cell proliferation kit was used to examine SRA01/04 cell proliferation; Transwell system and scratch assays were used to observe cell migration; and qPCR and western blot analysis were used to e...
Source: Acta Biochimica et Biophysica Sinica - September 20, 2018 Category: Biochemistry Authors: Zhang Z, Zhu H, Liu Y, Quan F, Zhang X, Yu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Perturbations of Neuron-Restrictive Silencing Factor Modulate Corticotropin-Releasing Hormone Gene Expression in the Human Cell Line BeWo
Stress exacerbates disease, and understanding its molecular mechanisms is crucial to the development of novel therapeutic interventions to combat stress-related disorders. The driver of the stress response in the hypothalamic-pituitary-adrenal axis (HPA) is corticotropin-releasing hormone (CRH), a neuropeptide synthesized in the paraventricular nucleus of the hypothalamus. Evidence supports that CRH expression is epigenetically modified at the molecular level by environmental stimuli, causing changes in the stress response. This effect is mediated by a concert of factors that translate environmental change into alterations...
Source: Molecular Neuropsychiatry - September 19, 2018 Category: Neuroscience Source Type: research

Capn4 expression is modulated by microRNA-520b and exerts an oncogenic role in prostate cancer cells by promoting Wnt/β-catenin signaling
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Wei Ren, Dong Wang, Chan Li, Tao Shu, Wei Zhang, Xiaoliang FuAbstractAccumulating evidence reports that Capn4 plays an important role in the development and progression of various malignant cancers. However, whether Capn4 is involved in prostate cancer remains unclear. Therefore, the aim of this study was to investigate the expression, biological function and regulatory mechanism of Capn4 in prostate cancer. Herein, we found that Capn4 was highly expressed in prostate cancer cell lines compared with normal prostate cells. Capn4 gene ...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

c-Fos/microRNA-18a feedback loop modulates the tumor growth via HMBOX1 in human gliomas
In conclusion, our study determine that c-Fos/miR-18a feedback loop promotes the tumor growth of gliomas by HMBOX1, providing important clues for understanding the key roles of transcription factor mediated mRNA-miRNA functional network in the regulation of gliomas.Graphical abstract
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

DIXDC1 promotes the growth of acute myeloid leukemia cells by upregulating the Wnt/β-catenin signaling pathway
In this study, we aimed to investigate the expression status and potential biological function of DIXDC1 in AML. Our results showed that DIXDC1 expression was highly upregulated in AML cell lines and primary AML blasts compared with normal blasts. Knockdown of DIXDC1 by siRNA-mediated gene silencing significantly inhibited proliferation, induced cell cycle arrest, and promoted apoptosis of AML cells in vitro. By contrast, DIXDC1 overexpression promoted proliferation, accelerated cell cycle progression, and reduced apoptosis of AML cells. Moreover, we found that DIXDC1 knockdown decreased the expression of β-catenin and re...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

The silencing of ApoC3 suppresses oxidative stress and inflammatory responses in placenta cells from mice with preeclampsia via inhibition of the NF-κB signaling pathway
ConclusionSilencing ApoC3 could suppress the NF-κB signaling pathway, thereby exercising a protective effect on cell injury induced by oxidative stress and reducing inflammatory responses.Graphical abstractThe molecular mechanism involving silenced APOC3 regulating the oxidative stress and inflammatory responses in placenta cells of preeclampsia mice via inhibition of the NF-κB signaling pathway. In preeclampsia mice, up-regulated APOC3 increased the expression of p65 and inhibited the expression of phosphorylation of IkBα, thus resulting in the oxidative stress injury and inflammatory responses of placenta cells. While...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Green tea compound helps siRNA slip inside cells
(American Chemical Society) Drinking green tea has been linked to health benefits ranging from cardiovascular disease prevention to weight loss. Although many of these claims still need to be verified in the clinic, an antioxidant in green tea called epigallocatechin gallate (EGCG) appears to have beneficial effects in cells and animals. Now, researchers have found a surprising use for EGCG: sneaking therapeutic RNAs into cells. They report their results in ACS Central Science.
Source: EurekAlert! - Medicine and Health - September 19, 2018 Category: International Medicine & Public Health Source Type: news

Palmitate aggravates proteinuria-induced cell death and inflammation via CD36-inflammasome axis in the proximal tubular cells of obese mice.
In this study, we investigated the roles of CD36 and NLRP3 inflammasomes in FFA-induced renal injury in high-fat diet (HFD)-induced obesity. HFD-fed C57BL/6 mice and palmitate-treated HK2 renal tubular cells were used as in vivo and in vitro models. Immunohistochemical staining showed that CD36, IL-1β, and IL-18 levels increased progressively in the kidneys of HFD-fed mice. Sulfo-N-succinimidyl oleate (SSO), a CD36 inhibitor, attenuated the HFD-induced upregulation of NLRP3, IL-1β, and IL-18 and suppressed the colocalization of NLRP3 and ASC in renal tubular cells. In vitro, SSO abolished the palmitate-induced activation...
Source: American Journal of Physiology. Renal Physiology - September 19, 2018 Category: Physiology Authors: Li LC, Yang JL, Lee WC, Chen JB, Lee CT, Wang PW, Vaghese Z, Chen WY Tags: Am J Physiol Renal Physiol Source Type: research

LncRNA-135528 inhibits tumor progression by up-regulating CXCL10 through the JAK/STAT pathway
AbstractSpontaneous tumor regression can be observed in many tumors, however, studies related to the altered expression of lncRNA in spontaneous glioma regression are limited, and the potential contributions of lncRNAs to spontaneous glioma regression remain unknown. To investigate the biological roles of lncRNA-135528 in spontaneous glioma regression. The cDNA fragment of lncRNA-135528 was obtained by rapid-amplification of cDNA ends (RACE) technology and cloned into the plvx-mcmv-zsgreen-puro vector. Additionally, we stably silenced or overexpressed lncRNA-135528 in G422 cells by transfecting with siRNA against lncRNA-13...
Source: Apoptosis - September 19, 2018 Category: Molecular Biology Source Type: research

Fibroblast growth factor 19 improves cardiac function and mitochondrial energy homoeostasis in the diabetic heart.
In this study, we investigated the effects of FGF19 on energy metabolism. FGF19 treatment of diabetic hearts exhibited higher glucose uptake and lower lipid profiles, suggesting changes in energy metabolism. The protective effects of FGF19 prevented ventricular dysfunction in diabetic hearts and improved mitochondrial function by the upregulation of PGC-1α expression. On the other side, knockdown of PGC-1α by siRNA attenuated the effects of FGF19 on the enhancement of mitochondrial function and energy efficiency. Taken together, these results show that FGF19 exhibited improved mitochondrial efficiency, which might be ass...
Source: Biochemical and Biophysical Research communications - September 19, 2018 Category: Biochemistry Authors: Xu W, Wang Y, Guo Y, Liu J, Ma L, Cao W, Yu B, Zhou Y Tags: Biochem Biophys Res Commun Source Type: research

A highly selective fluorescent probe for detecting glutathione transferases to reveal anticancer-activity sensitivity of cisplatin in cancer cells and tumor tissues
Publication date: 20 December 2018Source: Sensors and Actuators B: Chemical, Volume 277Author(s): Xiangge Tian, Zhenhao Tian, Yan Wang, Jie Hou, Lei Feng, Liang Song, Jingnan Cui, Xiaokui Huo, Xinyu Zhao, Dayong Zhou, Chao Wang, Chengpeng Sun, Jing Ning, Xiaochi MaAbstractCisplatin is a widely used anti-cancer drug in clinic, while many cancer cells gradually exhibited serious cisplatin resistance recently, which resulted in reducing its clinical effects. Glutathione transferases (GSTs) as an extensive expressed metabolic enzyme in human, plays a significant role in the metabolism of cisplatin. Thus, sensitively and accura...
Source: Sensors and Actuators B: Chemical - September 18, 2018 Category: Chemistry Source Type: research

Inhibition of endoplasmic reticulum stress by intermedin1-53 attenuates angiotensin II –induced abdominal aortic aneurysm in ApoE KO Mice
In conclusion, IMD may protect against AAA formation by inhibiting ERS via activating AMPK phosphorylation.
Source: Endocrine - September 18, 2018 Category: Endocrinology Source Type: research

The vimentin intermediate filament network restrains regulatory T cell suppression of graft-versus-host disease
Regulatory T cells (Tregs) are critical for maintaining immune homeostasis. However, current Treg immunotherapies do not optimally treat inflammatory diseases in patients. Understanding the cellular processes that control Treg function may allow for the augmentation of therapeutic efficacy. In contrast to activated conventional T cells, in which protein kinase C-θ (PKC-θ) localizes to the contact point between T cells and antigen-presenting cells, in human and mouse Tregs, PKC-θ localizes to the opposite end of the cell in the distal pole complex (DPC). Here, using a phosphoproteomic screen, we identified the intermedia...
Source: Journal of Clinical Investigation - September 18, 2018 Category: Biomedical Science Authors: Cameron McDonald-Hyman, James T. Muller, Michael Loschi, Govindarajan Thangavelu, Asim Saha, Sudha Kumari, Dawn K. Reichenbach, Michelle J. Smith, Guoan Zhang, Brent H. Koehn, Jiqiang Lin, Jason S. Mitchell, Brian T. Fife, Angela Panoskaltsis-Mortari, Col Source Type: research

DNMT1, a Novel Regulator Mediating mTORC1/mTORC2 Pathway-Induced NGF Expression in Schwann Cells.
Abstract Schwann cells play an important role in maintaining the normal function of peripheral nerves via the secretion of nerve growth factor (NGF). The mTOR signaling pathway is known as a kind of Ser/Thr protein kinase that regulates various cell functions. DNA methyltransferase 1 (DNMT1) is an epigenetic regulator and downstream target of the mTOR pathway. In the present study, we explored the relationship between NGF expression and the mTOR pathway/DNMT1 in RSC96 cells. The results showed that both rapamycin and Torin 1 downregulated NGF expression via the inhibition of phospho-mTOR (Ser 2448) and phospho-S6K...
Source: Neurochemical Research - September 18, 2018 Category: Neuroscience Authors: Cheng M, Lv X, Zhang C, Du W, Liu Y, Zhu L, Hao J Tags: Neurochem Res Source Type: research

Decreased expression of LATS1 correlates with astrogliosis after spinal cord injury.
Abstract Large tumor suppressor kinase 1 (LATS1) is a serine/threonine kinase of the AGC kinase family in mammals and involved in various biological processes, it is a key regulator of cell cycle progression. However, the role of LATS1 in central nervous system trauma is still unknown. In present study, we performed an acute spinal cord injury (SCI) model in adult rats and investigated the dynamic changes of LATS1 expression in the spinal cord. We found that LATS1 protein levels were significantly decreased at day 1 after injury. Meanwhile, double immunofluorescence staining showed these changes were striking in a...
Source: Biochemical and Biophysical Research communications - September 18, 2018 Category: Biochemistry Authors: Wang Y, Chen M Tags: Biochem Biophys Res Commun Source Type: research

CDC25B promotes influenza A virus replication by regulating the phosphorylation of nucleoprotein.
Abstract Cell division cycle 25 B (CDC25B) is a member of the CDC25 phosphatase family. It can dephosphorylate cyclin-dependent kinases and regulate the cell division cycle. Moreover, siRNA knockdown of CDC25B impairs influenza A virus (IAV) replication. Here, to further understand the regulatory mechanism of CDC25B for IAV replication, a CDC25B-knockout (KO) 293T cell line was constructed using CRISPR/Cas9. The present data indicated that the replication of IAV was decreased in CDC25B-KO cells. Additionally, CDC25B deficiency damaged viral polymerase activity, nucleoprotein (NP) self-oligomerization, and NP nucle...
Source: Virology - September 18, 2018 Category: Virology Authors: Cui L, Mahesutihan M, Zheng W, Meng L, Fan W, Li J, Ye X, Liu W, Sun L Tags: Virology Source Type: research

Phosphodiesterase 4 inhibitor activates AMPK-SIRT6 pathway to prevent aging-related adipose deposition induced by metabolic disorder.
Abstract Rolipram is a selective phosphodiesterase 4 (PDE4) inhibitor that exerts a variety of effects, including anti-inflammatory, immunosuppressive, and anti-tumor effects. The aim of this study was to investigate the effect of rolipram on metabolic disorder and its underlying mechanisms. Metabolic disorder was induced in 8-week-old wild type BABL/c mice by administration of D-galactose for 4 weeks. Simultaneously the mice were administered vehicle or rolipram. Alternatively, beginning at 3 or 21 months, the mice were administered db-cAMP for 3 months, with or without a high-fat-diet (HFD) to induce metabolic d...
Source: Aging - September 18, 2018 Category: Biomedical Science Authors: Wang Z, Liang Y, Zhang L, Zhang N, Liu Q, Wang Z Tags: Aging (Albany NY) Source Type: research

Proliferation Potential-Related Protein Promotes the Esophageal Cancer Cell Proliferation, Migration and Suppresses Apoptosis by Mediating the Expression of p53 and Interleukin-17
Conclusion: Our data suggest that PP-RP promotes esophageal cancer cell proliferation and migration, and suppresses apoptosis by mediating the expression of p53 and IL-17.Pathobiology
Source: Pathobiology - September 17, 2018 Category: Pathology Source Type: research

ASAP Charge and Assembly Reversible Micelles Fueled by Intracellular ATP for Improved siRNA Transfection
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.8b13300
Source: ACS Applied Materials and Interfaces - September 17, 2018 Category: Materials Science Authors: Zhanwei Zhou, Chenzi Li, Minghua Zhang, Qingyan Zhang, Chenggen Qian, David Oupicky, Minjie Sun Source Type: research

Additive polyplexes to undertake siRNA therapy against CDC20 and survivin in breast cancer cells.
Abstract Small interfering RNA (siRNA) delivered to silence overexpressed genes associated with malignancies is a promising targeted therapy to decrease the uncontrolled growth of malignant cells. To create potent delivery agents for siRNA, here we formulated additive polyplexes of siRNA using linoleic acid-substituted polyethylenimine and additive polymers (hyaluronic acid, polyacrylic acid, dextran sulfate and methyl cellulose), and characterized their physicochemical properties and effectiveness. Incorporating polyanionic polymer along with anionic siRNA in polyplexes was found to decrease the ζ-potential of p...
Source: Biomacromolecules - September 17, 2018 Category: Biochemistry Authors: Parmar MB, K C RB, Löbenberg R, Uludag H Tags: Biomacromolecules Source Type: research

MicroRNA-338-5p plays a tumor suppressor role in glioma through inhibition of the MAPK-signaling pathway by binding to FOXD1
ConclusionWe found that overexpression of miR-338-5p suppresses glioma cell proliferation, migration, and invasion and accelerates its senescence and apoptosis by decreasing FOXD1 expression via inhibition of activation of MAPK-signaling pathway.
Source: Journal of Cancer Research and Clinical Oncology - September 17, 2018 Category: Cancer & Oncology Source Type: research

GSE116188 Transcriptional profiling of WM1976 cells with siRNA knockdown of the long non-coding RNA SLNCR
Contributors : Karyn Schmidt ; Carl NovinaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRNA-Seq was used to profile transcriptional changes induced by siRNA knockdown of the long non-coding RNA SLNCR (all isoforms).
Source: GEO: Gene Expression Omnibus - September 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Methotrexate induced cell death mechanisms in MCF-7 adenocarcinoma breast cancer cells: Enhanced cytotoxicity following dff45-siRNA pre-treatment
Publication date: Available online 30 August 2018Source: SynergyAuthor(s): Fatemeh Kiani, Negin Rasouli, Tahereh Kashkoolinejad, Shahrokh Safarian, Seyed Jalal Zargar, Nader SheibaniAbstractThere are many efforts to diminish risks associated with the use of cancer chemotherapeutic agents through design and utilization of new strategies including gene therapy. We previously showed knockdown of dff45 or overexpression of dff40, the important apoptosis regulators, resulted in enhanced sensitization of cancer cells to chemotherapeutic agents. Here we show that pre-treatment with dff45-siRNA additively increased the overall cyt...
Source: Synergy - August 31, 2018 Category: Molecular Biology Source Type: research

Lipid-based siRNA Nanodelivery Systems: a Learning Process for Improving Transfer from Concepts to Clinical Applications.
CONCLUSION: Formulation design should be increasingly addressed with industrial criteria; it should be based on quality by design and on the estimation of critical attributes that affect performance, and supported by a range of characterization techniques and appropriate analytical methods. PMID: 30160217 [PubMed - as supplied by publisher]
Source: Current Clinical Pharmacology - August 29, 2018 Category: Drugs & Pharmacology Authors: Perez SE, Carlucci AM Tags: Curr Clin Pharmacol Source Type: research

Dendrimer as a new potential carrier for topical delivery of siRNA: a comparative study of dendriplex vs. lipoplex for delivery of TNF-α siRNA
Publication date: Available online 27 August 2018Source: International Journal of PharmaceuticsAuthor(s): Palpandi Pandi, Anjali Jain, Nagavendra Kommineni, Maksim Ionov, Maria Bryszewska, Wahid KhanAbstractTopical delivery of siRNA is challenging task due to complex barrier property of stratum corneum and cationic lipid based carriers have been widely explored for this purpose due to improved permeation through skin. For gene delivery application, dendrimers are considered as efficient carrier due to their cationic nature and well-defined surface groups. However, they are not well explored for topical delivery. This work ...
Source: International Journal of Pharmaceutics - August 28, 2018 Category: Drugs & Pharmacology Source Type: research