Query: siRNA

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Total 17011 results found since Jan 2013.

High Mobility Group Box 1 Regulates Uterine Decidualization through Bone Morphogenetic Protein 2 and Plays a Role in Kruppel-Like Factor 5-Induced Stromal Differentiation
Conclusion: Hmgb1 may play an important role during mouse uterine decidualization.Cell Physiol Biochem 2018;48:2399 –2408
Source: Cellular Physiology and Biochemistry - August 17, 2018 Category: Cytology Source Type: research

Study on Regulation of Low Density Lipoprotein Cholesterol Metabolism using PCSK9 Gene Silencing: A computational Approach.
Abstract Combating and preventing abnormality in lipid metabolism becomes a pivotal criterion for research. Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a circulating protein; it promotes the degradation of low-density lipoprotein receptors (LDL-R) and hence increases LDL-C levels. Silencing the gene PCSK9 at post-transcriptional level with the help of small interfering Ribo nucleic acid (siRNA) gives a new insight and a novel therapeutic way to regulate LDL-C metabolism. Designing and selecting an efficient siRNA for silencing PCSK9 at post transcriptional level through computational approach. We have...
Source: Bioinformation - August 17, 2018 Category: Bioinformatics Authors: Vijayaraghavan B, Danabal K, Padmanabhan G, Ramanathan K Tags: Bioinformation Source Type: research

GSE118642 ANXA10 induction by interaction with tumor-associated macrophages promotes the growth of esophageal squamous cell carcinoma
Contributors : Himiko Kodaira ; Hiroshi YokozakiSeries Type : Expression profiling by arrayOrganism : Homo sapiensTumor-associated macrophages (TAMs) have important roles in the progression, angiogenesis and motility of various cancers. To study the effects of TAMs on the tumor microenvironment of ESCCs, we constructed an in vitro system for the differentiation of peripheral blood monocyte (PBMo)-derived macrophages into TAM-like PBMo-derived macrophages.We established a co-culture assay using a human ESCC cell line and TAM-like PBMo-derived macrophages and we performed a cDNA microarray analysis between monocultured and c...
Source: GEO: Gene Expression Omnibus - August 17, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE113369 CDK4 inhibition diminishes p53 activation by Mdm2-antagonists
Contributors : Matthias Dobbelstein ; Anusha SriramanSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCDK4 inhibitors have reached clinical approval for cancer therapy. In parallel, the p53 antagonist Mdm2 remains an attractive target for anti-cancer therapy, including numerous clinical studies. The genes encoding Mdm2 and CDK4 are frequently co-amplified in human malignancies, most notably in liposarcomas, suggesting their combined targeting for therapy. Here we show, however, that small compounds that inhibit Mdm2 and CDK4 antagonize each other rather than synergize in their cytotoxi...
Source: GEO: Gene Expression Omnibus - August 17, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Cloning, expression analysis and functional characterization of an interleukin-1 receptor-associated kinase 4 from Apostichopus japonicus
In this study, an IRAK4 homologue molecule was identified from Apostichopus japonicus (designated as AjIRAK4) by RACE approach. The full-length cDNA of AjIRAK4 was of 2024 bp containing an open reading frame of 1311 bp encoding a 436-amino-acid (aa) residue polyprotein with the typical death domain (10-113aa) and the kinase domain (160-426aa). The mRNA transcripts of AjIRAK4 displayed constitutively expressed in all examined tissues with highest expression in the muscles (7.20-fold increase compared to the coelomocytes). The pathogen Vibrio splendidus challenge and LPS exposure could both significantly up-regulate the mRNA...
Source: Molecular Immunology - August 17, 2018 Category: Allergy & Immunology Source Type: research

Ethanol Extract of Lycopus lucidus Turcz. ex Benth Inhibits Metastasis by Downregulation of Runx-2 in Mouse Colon Cancer Cells.
This study aims to elucidate the effects of ethanol extracts of LT (ELT) relative to the role of Runt-related transcription factor- (Runx-) 2 in the invasive and metastatic potentials of mouse colon cancer to determine the underlying mechanisms involved. ELT was evaluated for the antimetastasis activity using CT-26 colon cancer using wound healing, transwell matrigel, and western blot analysis. We used Runx-2-specific siRNA to further determine the relationship between Runx-2 and matrix metalloprotease- (MMP-) 9 in the migration and invasion of CT-26 cells. Runx-2 was first demonstrated to be a transcription factor that pl...
Source: Evidence-based Complementary and Alternative Medicine - August 16, 2018 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

Beta-secretase/BACE1 Promotes APP Endocytosis and Processing in the Endosomes and on Cell Membrane
Publication date: Available online 16 August 2018Source: Neuroscience LettersAuthor(s): Mingguang Wang, Tian Jing, Xuan Wang, Dan YaoAbstractAmyloid-β proteins deposition and aggregation occur in extracellular space and form neuritic plaques in Alzheimer’s disease (AD) brain. Beta-site amyloid precursor protein cleaving enzyme 1 (BACE1)/ β-secretase and γ-secretase Presenilin 1 (PSEN1) conduct sequential cleavage of amyloid- β precursor protein (APP) and yield amyloid- β proteins. However the details of the interactions of APP with the enzymes and transportation of catalytic products are unclear. Here we reveal dist...
Source: Neuroscience Letters - August 16, 2018 Category: Neuroscience Source Type: research

ASAP pH-Sensitive Nanocarrier-Mediated Codelivery of Simvastatin and Noggin siRNA for Synergistic Enhancement of Osteogenesis
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.8b10521
Source: ACS Applied Materials and Interfaces - August 16, 2018 Category: Materials Science Authors: Jinsheng Huang, Chaowen Lin, Jintao Fang, Xiaoxia Li, Jin Wang, Shaohui Deng, Sheng Zhang, Wanhan Su, Xiaoreng Feng, Bin Chen, Du Cheng, Xintao Shuai Source Type: research

miR ‑197 promotes the invasion and migration of colorectal cancer by targeting insulin‑like growth factor‑binding protein 3.
In conclusion, we revealed that miR‑197 modulates IGFBP3 and therefore plays a critical role in regulating CRC migration and invasion. PMID: 30106114 [PubMed - as supplied by publisher]
Source: Oncology Reports - August 16, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Inhibitor of apoptosis protein ‑like protein‑2: A novel growth accelerator for breast cancer cells.
Inhibitor of apoptosis protein‑like protein‑2: A novel growth accelerator for breast cancer cells. Oncol Rep. 2018 Aug 03;: Authors: Zhu L, Zhou W, Zhu X, Xiang S, Wang S, Peng Y, Lu B, Tang P, Chen Q, Wu M, Peng X, Chen Z, Sun Z, Yang K, Xiang M, Yu D Abstract Although the inhibitor of apoptosis protein‑like protein‑2 (ILP‑2) has been shown as a serological biomarker for breast cancer, its effect on breast cancer cell growth remains elusive. The present study aimed to determine the role of ILP‑2 in breast cancer cell growth. We used immunohistochemistry to analyze ILP‑2 expression in 59 ...
Source: Oncology Reports - August 16, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

MicroRNA ‑34b promotes proliferation, migration and invasion of Ewing's sarcoma cells by downregulating Notch1.
In conclusion, EWS‑FLI1 may modulate miR‑34b expression directly or indirectly, and miR‑34b potentially has an oncogenic role in Ewing's sarcoma by downregulating Notch1. PMID: 30106161 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - August 16, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Correlation between DEC1/DEC2 and epithelial ‑mesenchymal transition in human prostate cancer PC‑3 cells.
Correlation between DEC1/DEC2 and epithelial‑mesenchymal transition in human prostate cancer PC‑3 cells. Mol Med Rep. 2018 Aug 09;: Authors: Liu Q, Wu Y, Seino H, Haga T, Yoshizawa T, Morohashi S, Kijima H Abstract Differentiated embryonic chondrocyte (DEC) genes have been reported to be involved in the regulation of mammalian circadian rhythms, differentiation, apoptosis, the response to hypoxia and epithelial‑mesenchymal transition (EMT). Activation of transforming growth factor (TGF)‑β signaling is known to promote EMT for the development of metastatic castration‑resistant prostate cancer...
Source: Molecular Medicine Reports - August 16, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Anti-TLR2 Immunotherapy as a Potential Treatment for Synucleinopathies
The protein α-synuclein, like amyloid-β and tau, forms into an increasing amount of solid aggregates in the aging brain. These aggregates and their surrounding biochemistry cause neural dysfunction and cell death. Every older individual has raised levels of these protein aggregates, and the pathology they cause rises to the level of named condition when one or another is present in great enough amounts. The age-related diseases associated with α-synuclein are termed synucleinopathies, though one can argue that all neurodegeneration is to some degree influenced by all protein aggregates. Where the research, medical, and ...
Source: Fight Aging! - August 15, 2018 Category: Research Authors: Reason Tags: Medicine, Biotech, Research Source Type: blogs

GSE98514 Expression profiling of four replicates of MCF-7 cells treated with 100nM TCDD
Contributors : Sean Piwarski ; James Denvir ; Donald A Primerano ; Jun Fan ; Travis B SalisburySeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that binds pollutants, therapeutic drugs and endogenous ligands. The AHR is expressed in all breast cancer subtypes and it can switch the aggressiveness of breast cancer cells from low to high depending on the ligand that it binds. Jagged 1 (JAG1) is a NOTCH receptor ligand that is overexpressed in basal-like breast cancer. JAG1 promotes breast cancer progression in ...
Source: GEO: Gene Expression Omnibus - August 15, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Tunability of Biodegradable Poly(amine-co-ester) Polymers for Customized Nucleic Acid Delivery and Other Biomedical Applications.
Abstract Gene therapy promises to treat diseases that arise from genetic abnormalities by correcting the underlying cause of the disease rather than treating the associated symptoms. Successful transfer of nucleic acids into cells requires efficient delivery vehicles that protect the cargo and can penetrate the appropriate cellular barriers before releasing their contents. Many viral vectors and synthetic polycationic vectors for nucleic acid delivery do not translate well from in vitro to in vivo applications due to their instability and toxicity. We synthesized and characterized a library of biocompatible low ch...
Source: Biomacromolecules - August 15, 2018 Category: Biochemistry Authors: Kauffman AC, Piotrowski-Daspit AS, Nakazawa KH, Jiang Y, Datye A, Saltzman WM Tags: Biomacromolecules Source Type: research

Lamp2 inhibits epithelial-mesenchymal transition by suppressing Snail expression in HCC.
This study found that Lamp2 expression was significantly lower in HCC tissues than in adjacent nontumor tissues (ANTs), and its expression level correlated with HCC metastasis. Low Lamp2 expression was significantly correlated with the AFP serum level (> 20 ng/Ml, P = 0.024), capsular formation (absent, P = 0.024), and microvascular invasion (present, P < 0.001), and low expression of Lamp2 indicated a poor prognosis in HCC. LowLamp2 expression was an independent and significant risk factor for recurrence-free survival (RFS; P < 0.001) and overall survival (OS; P < 0.001) in HCC. In this study, we demonstrated ...
Source: Oncotarget - August 14, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

GSE102653 Genome wide expression change by SMURF1 knocking down in MCF-7 cells
Contributor : J ZhuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe aim to the investigate the role of SMURF1 in breast cancer progression. MCF-7 cells were used as the model and SMURF1 was silenced by siRNA.
Source: GEO: Gene Expression Omnibus - August 14, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Fragile Histidine Triad (FHIT), a Novel Modifier Gene in Pulmonary Arterial Hypertension.
CONCLUSIONS: This study highlights the importance of the novel BMPR2 modifier FHIT in PH and the clinical value of the repurposed drug Enzastaurin as a potential novel therapeutic strategy to improve PAH. PMID: 30107138 [PubMed - as supplied by publisher]
Source: Am J Respir Crit Car... - August 14, 2018 Category: Intensive Care Authors: Dannewitz Prosseda S, Tian X, Kuramoto K, Boehm M, Sudheendra D, Miyagawa K, Zhang F, Solow-Cordero D, Saldivar JC, Austin ED, Loyd JE, Wheeler L, Andruska A, Donato M, Wang L, Huebner K, Metzger RJ, Khatri P, Spiekerkoetter E Tags: Am J Respir Crit Care Med Source Type: research

Molecules, Vol. 23, Pages 2028: Evaluation of Amino-Functional Polyester Dendrimers Based on Bis-MPA as Nonviral Vectors for siRNA Delivery
Malkoch Herein, we present the first evaluation of cationic dendrimers based on 2,2-bis(methylol)propionic acid (bis-MPA) as nonviral vectors for transfection of short interfering RNA (siRNA) in cell cultures. The study encompassed dendrimers of generation one to four (G1&amp;ndash;G4), modified to bear 6&amp;ndash;48 amino end-groups, where the G2&amp;ndash;G4 proved to be capable of siRNA complexation and protection against RNase-mediated degradation. The dendrimers were nontoxic to astrocytes, glioma (C6), and glioblastoma (U87), while G3 and G4 exhibited concentration dependent toxicity towards primary ...
Source: Molecules - August 14, 2018 Category: Chemistry Authors: Patrik Stenstr öm Dario Manzanares Yuning Zhang Valentin Ce ña Michael Malkoch Tags: Article Source Type: research

JAGN1 is required for fungal killing in neutrophil extracellular traps: Implications for severe congenital neutropenia.
Abstract Mutations in the gene JAGN1 were recently discovered in patients with severe congenital neutropenia (SCN). Neutrophils release neutrophil extracellular traps (NETs) consisting of decondensed chromatin decorated with various granular proteins such as neutrophil elastase and myeloperoxidase (MPO) to combat microbial infections. However, whether JAGN1 is required for the formation or function of NETs is not known. Here, we analyzed primary neutrophils from a patient with homozygous JAGN1 mutations with respect to phorbol myristate acetate (PMA)-induced NET formation. NET release was observed, but there appea...
Source: Journal of Leukocyte Biology - August 14, 2018 Category: Hematology Authors: Khandagale A, Lazzaretto B, Carlsson G, Sundin M, Shafeeq S, Römling U, Fadeel B Tags: J Leukoc Biol Source Type: research

Functional coupling of GABAA/B receptors and the channel TRPV4 mediates rapid progesterone signaling in the oviduct.
We report a rapid response to P4 and agonists of γ-aminobutyric acid receptors A and B (GABAA/B) in the mouse oviduct that was characterized by oscillatory Ca2+ signals and increased ciliary beat frequency (CBF). Pharmacological manipulation, genetic ablation, and siRNA-mediated knockdown in oviductal cells, as well as overexpression experiments in HEK 293T cells, confirmed the participation of the cationic channel TRPV4, different subunits of GABAA (α1 to α3, β2, and β3), and GABAB1 in P4-induced responses. TRPV4-mediated Ca2+ entry in close proximity to the inositol trisphosphate receptor was required to initiate an...
Source: Science Signaling - August 14, 2018 Category: Biomedical Science Authors: Jung C, Fernández-Dueñas V, Plata C, Garcia-Elias A, Ciruela F, Fernández-Fernández JM, Valverde MA Tags: Sci Signal Source Type: research

Functional coupling of GABAA/B receptors and the channel TRPV4 mediates rapid progesterone signaling in the oviduct
We report a rapid response to P4 and agonists of -aminobutyric acid receptors A and B (GABAA/B) in the mouse oviduct that was characterized by oscillatory Ca2+ signals and increased ciliary beat frequency (CBF). Pharmacological manipulation, genetic ablation, and siRNA-mediated knockdown in oviductal cells, as well as overexpression experiments in HEK 293T cells, confirmed the participation of the cationic channel TRPV4, different subunits of GABAA (α1 to α3, β2, and β3), and GABAB1 in P4-induced responses. TRPV4-mediated Ca2+ entry in close proximity to the inositol trisphosphate receptor was require...
Source: Signal Transduction Knowledge Environment - August 14, 2018 Category: Science Authors: Jung, C., Fernandez-Duenas, V., Plata, C., Garcia-Elias, A., Ciruela, F., Fernandez-Fernandez, J. M., Valverde, M. A. Tags: STKE Research Articles Source Type: news

Silencing of PHLPP1 promotes neuronal apoptosis and inhibits functional recovery after spinal cord injury in mice
In this study, we investigated the effects of the progression of SCI and potential regulation of apoptosis after the Pleckstrin homology (PH) domain and leucine rich repeat protein phosphatase 1 (PHLPP1) gene was silenced.Main methodsSpinal cord injection, and neuronal transfection with a recombinant adenovirus vector encoding small interfering RNA (siRNA) against PHLPP1 (AdsiPHLPP1) successfully silenced PHLPP1. These created in vivo and in vitro PHLPP1-silenced models, respectively, resulting in stable expression of the transgene in neurons.Key findingsThe results showed that silencing of PHLPP1 evidently reduced levels ...
Source: Life Sciences - August 13, 2018 Category: Biology Source Type: research

15-lipoxygenase-2/15(S)-hydroxyeicosatetraenoic acid regulates cell proliferation and metastasis via the STAT3 pathway in lung adenocarcinoma
This study may provide a greater understanding of LAC metastasis and shed new light on the mechanisms by which the 15(S)-HETE/STAT3 pathway is related to this disease.
Source: Prostaglandins and Other Lipid Mediators - August 13, 2018 Category: Lipidology Source Type: research

Characterizing the interaction between insulin-like growth factor 2 mRNA-binding protein 1 (IMP1) and KRAS expression.
This study shows that the regulation of KRAS expression by IMP1 is complex and may involve both the IMP1 protein as well as its mRNA transcript. PMID: 30104206 [PubMed - as supplied by publisher]
Source: The Biochemical Journal - August 13, 2018 Category: Biochemistry Authors: Mackedenski S, Wang C, Li WM, Lee CH Tags: Biochem J Source Type: research

Fos-like antigen 2 (FOSL2) promotes metastasis in colon cancer.
Abstract Among different cancers, incidence and mortality of colorectal cancer (CRC) is one of the highest. KRAS mutation is one of the underlying features in the pathogenesis of CRC with CRC tumors harboring mutant KRAS exhibiting a more aggressive behavior compared to CRC tumors with wild type KRAS. We had earlier shown that the microRNA-143 (miR-143) replenishment not only chemosensitizes CRC cell line with mutant KRAS instead of wild-type KRAS gene, to paclitaxel-mediated cytotoxicity, but also inhibits cell migration and invasion ability. Hence, the study aimed to determine how miR-143 replenishment is inhibi...
Source: Experimental Cell Research - August 13, 2018 Category: Cytology Authors: Fei BY, Wang XY, Fang XD Tags: Exp Cell Res Source Type: research

Rab27a Regulates Human Perivascular Adipose Progenitor Cell Differentiation
ConclusionsPVAT-APC from CABG donors express common adipocyte progenitor markers and differentiate into UCP1-containing adipocytes. Rab27a has an endogenous role in promoting the maturation of adipocytes from human PVAT-derived APC.
Source: Cardiovascular Drugs and Therapy - August 13, 2018 Category: Cardiology Source Type: research

Bioreducible Poly(Amino Ethers) Based mTOR siRNA Delivery for Lung Cancer
ConclusionsThe results demonstrate that the mPAE polymer based NPs show strong potential for siRNA delivery to lung cancer cells. It is anticipated that future modification can help improve the efficacy of nucleic acid delivery, leading to higher inhibition of lung cancer growthin vitro andin vivo.
Source: Pharmaceutical Research - August 13, 2018 Category: Drugs & Pharmacology Source Type: research

SRPK1 gene silencing promotes vascular smooth muscle cell proliferation and vascular remodeling via inhibition of the PI3K/Akt signaling pathway in a rat model of intracranial aneurysms.
CONCLUSION: Our results show that siRNA-mediated silencing of SRPK1 gene inhibits VSMC apoptosis, and increases VSMCs proliferation and vascular remodeling in IA via the PI3 K/Akt signaling pathway. Our findings provide a novel intervention target for the molecular treatment of IA. PMID: 30101479 [PubMed - as supplied by publisher]
Source: CNS Neuroscience and Therapeutics - August 12, 2018 Category: Neuroscience Authors: Li XG, Wang YB Tags: CNS Neurosci Ther Source Type: research

SIRT1-dependent epigenetic regulation of H3 and H4 histone acetylation in human breast cancer.
In this study, we elucidated SIRT1 epigenetic role and analyzed the link between the latter and histones H3 and H4 epigenetic marks in all 5 molecular subtypes of breast cancer. Using a cohort of 135 human breast tumors and their matched normal tissues, as well as 5 human-derived cell lines, we identified H3k4ac as a new prime target of SIRT1 in breast cancer. We also uncovered an inverse correlation between SIRT1 and the 3 epigenetic marks H3k4ac, H3k9ac and H4k16ac expression patterns. We showed that SIRT1 modulates the acetylation patterns of histones H3 and H4 in breast cancer. Moreover, SIRT1 regulates its H3 acetylat...
Source: Oncotarget - August 11, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

The nuclear-cytoplasmic trafficking of a chromatin-modifying and remodelling protein (KMT2C), in osteosarcoma.
Authors: Chiappetta C, Puggioni C, Carletti R, Petrozza V, Della Rocca C, Di Crisfofano C Abstract Osteosarcoma is the most common paediatric primary non-hematopoietic bone tumor; the survival is related to the response to chemotherapy and development of metastases. KMT2C is a chromatin-modifying and remodelling protein and its expression has never been studied in osteosarcoma. The aim of this study was to understand the role of KMT2C in the osteosarcoma carcinogenesis and metastatic progression to identify a new molecular target and to provide new therapeutic approach. We performed the immunohistochemical and gene...
Source: Oncotarget - August 11, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Osmotic and hypoxic induction of the complement factor C9 in cultured human retinal pigment epithelial cells: Regulation of VEGF and NLRP3 expression.
Conclusions: The expression of the C9 gene in cultured RPE cells is highly induced by extracellular hyperosmolarity, hypoxia, and oxidative stress. The data may support the assumption that C9 gene expression may stimulate the expression of inflammatory (NLRP3) and angiogenic growth factors (VEGF) in RPE cells. Extracellular C9 protein may attenuate this effect, in part via negative regulation of the C9 mRNA level. PMID: 30090015 [PubMed - in process]
Source: Molecular Vision - August 11, 2018 Category: Molecular Biology Tags: Mol Vis Source Type: research

SiRNA silencing of VEGF, IGFs, and their receptors in human retinal microvascular endothelial cells.
Authors: Nicolau Y, Bany-Mohammed F, Cai CL, Aranda JV, Beharry KD Abstract Vascular endothelial growth factor (VEGF) is a potent mitogen that regulates proliferation, migration, and tube formation of endothelial cells (EC). VEGF has recently become a target for severe retinopathy of prematurity (ROP) therapy. We tested the hypothesis that a specific VEGF isoform and/or receptor acts synergistically with insulin-like growth factor (IGF)-I to alter normal retinal microvascular EC angiogenesis and RNA interference can be used to reverse VEGF effects. We used small interfering RNA (SiRNA) transfection to target VEGF i...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Resveratrol alleviates sepsis-induced acute lung injury by suppressing inflammation and apoptosis of alveolar macrophage cells.
Authors: Yang L, Zhang Z, Zhuo Y, Cui L, Li C, Li D, Zhang S, Cui N, Wang X, Gao H Abstract Sepsis is a major cause of death in intensive care units. The purpose of this study was to investigate the effect of resveratrol (RSV) on sepsis-induced acute lung injury (ALI). The underlying molecular mechanisms were deciphered by both in vitro and in vivo experiments. Polymicrobial sepsis was induced in C57BL/6 mice by cecal ligation and puncture (CLP). RSV pretreatment significantly attenuated CLP-induced acute lung injury, which was associated with enhanced expression of VEGF-B. The protective properties of RSV were ass...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-449a suppresses hepatocellular carcinoma cell growth via G1 phase arrest and the HGF/MET c-Met pathway
ConclusionThe tumor suppressor miR-449a suppresses HCC tumorigenesis by repressing the c-Met/ERK pathway.
Source: Hepatobiliary and Pancreatic Diseases International - August 11, 2018 Category: Gastroenterology Source Type: research

Effects of Oncostatin M on Invasion of Primary Trophoblasts under Normoxia and Hypoxia Conditions.
CONCLUSION: OSM might be involved in the invasiveness of extravillous trophoblasts under hypoxia conditions via increasing MMP-2 and MMP-9 enzymatic activities through STAT3 signaling. Increased MMP-9 activity by OSM seems to be more important in primary trophoblasts. PMID: 30091322 [PubMed - in process]
Source: Yonsei Medical Journal - August 11, 2018 Category: Universities & Medical Training Authors: Wie JH, Ko HS, Choi SK, Park IY, Kim A, Kim HS, Shin JC Tags: Yonsei Med J Source Type: research

Inhibition of Epidermal Growth Factor Receptor (EGFR) Reduces Lipopolysaccharide (LPS)-Induced Activation and Inflammatory Cytokines in Hepatic Stellate Cells In Vitro.
CONCLUSIONS Activation of HSCs by LPS in vitro, including the expression of inflammatory cytokines and mediators of fibrogenesis, were shown to be dependent on the expression of EGFR. PMID: 30091424 [PubMed - in process]
Source: Medical Science Monitor - August 10, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Contribution of human UDP-glucuronosyltransferases to the antioxidant effects of propolis, artichoke and silymarin
Conclusion: In conclusion, this study provides a possible molecular mechanism for protective antioxidant effects associated with the herbal drugs propolis, artichoke and silymarin. The herbal drug-mediated transcriptional upregulation of human detoxifying UGT1A enzymes via activation of AhR and Nrf2 leads to reduced hydrogen peroxide and oxidative stress. Because of UGT1A activation, the intake of these drugs could affect the therapeutic efficacy of other drugs when these also undergo metabolism by glucuronidation.Graphical abstract
Source: Phytomedicine - August 10, 2018 Category: Drugs & Pharmacology Source Type: research

FDA approves first-of-its kind targeted RNA-based therapy to treat a rare disease
FDA approves new drug for treatment of polyneuropathy caused by hereditary transthyretin-mediated amyloidosis (hATTR). This is the first FDA-approved treatment for this rare, debilitating and often fatal genetic disease and the first FDA approval of a new class of drugs called small interfering ribonucleic acid (siRNA) treatment.
Source: Food and Drug Administration - August 10, 2018 Category: American Health Source Type: news

Mir-758-5p Suppresses Glioblastoma Proliferation, Migration and Invasion by Targeting ZBTB20
Conclusion: miR-758-5p suppressed GBM progression by targeting ZBTB20. The miR-758-5p/ZBTB20 axis might be a promising therapeutic target for GBM treatment.Cell Physiol Biochem 2018;48:2074 –2083
Source: Cellular Physiology and Biochemistry - August 10, 2018 Category: Cytology Source Type: research

High-degree and broad-spectrum resistance mediated by a combination of NIb siRNA and miRNA suppresses replication of necrotic and common strains of potato virus Y
In this study, we designed specific siRNAs and miRNAs to target two regions of the PVYO replicase gene (NIb). Eight plant expression vectors containing one or two sRNAs were constructed. Luciferase activity assays showed that the designed sRNAs successfully cleaved theNIb gene of PVYO and PVYN, and the vector carrying a combined siRNA- and miRNA-based short hairpin RNA (shRNA) demonstrated the strongest inhibitory effect. These effects were confirmed through the acquisition of PVYO and PVYN resistance in transgenic sRNA-expressingNicotiana tabacum plants. This phenomenon could be related to a plant defense mechanism in whi...
Source: Archives of Virology - August 10, 2018 Category: Virology Source Type: research

An enhanced RRM2 siRNA delivery to rheumatoid arthritis fibroblast-like synoviocytes through a liposome ‑protamine-DNA-siRNA complex with cell permeable peptides.
An enhanced RRM2 siRNA delivery to rheumatoid arthritis fibroblast-like synoviocytes through a liposome‑protamine-DNA-siRNA complex with cell permeable peptides. Int J Mol Med. 2018 Aug 08;: Authors: Wang X, Wang X, Sun J, Fu S Abstract Rheumatoid arthritis (RA) is considered to be a systemic autoimmune disease that induces systemic complications and progressive disability. It affects a large number of people. RA fibroblast‑like synoviocytes (RA‑FLS) promote the progression of RA through the secretion of proinflammatory cytokines and increasing invasiveness into the extracellular matrix. Therefo...
Source: International Journal of Molecular Medicine - August 8, 2018 Category: Molecular Biology Authors: Wang X, Wang X, Sun J, Fu S Tags: Int J Mol Med Source Type: research

Current non-viral siRNA delivery systems as a promissing treatment of skin diseases.
CONCLUSION: The treatment of skin diseases based on topical delivery of siRNA, which act by inhibiting the expression of target transcripts, offers many potential therapeutic advantages for suppressing genes into the skin. PMID: 30084329 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Design - August 7, 2018 Category: Drugs & Pharmacology Authors: Rosa J, Suzuki I, Kravicz M, Caron A, Pupo AV, Praca FG, Bentley MVLB Tags: Curr Pharm Des Source Type: research

A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle
ConclusionsThis study provides a minable data set for every human gene regarding its role in EBOV genome replication and transcription, shows that an FDA-approved drug targeting one of the identified pathways is highly efficacious in vitro, and demonstrates the power of life cycle modelling systems for conducting genome-wide host factor screens for BSL4 viruses.
Source: Genome Medicine - August 7, 2018 Category: Genetics & Stem Cells Source Type: research

Gene Silencing using siRNA for Preventing Liver Ischaemia-Reperfusion Injury.
CONCLUSION: siRNA therapeutic potential to preclude liver IRI can be improved by a better knowledge of what molecules to target and by using more efficient delivery strategies. PMID: 30084326 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Design - August 7, 2018 Category: Drugs & Pharmacology Authors: Marinho HS, Marcelino P, Soares H, Corvo ML Tags: Curr Pharm Des Source Type: research

Nanostructured Hyaluronic acid-based materials for the delivery of siRNA.
CONCLUSION: To overcome the problems associated with these emerging genetic tools, investigators have employed glycosaminoglycan HA-based biopolymers. This biopolymer offers a variety of properties such as biodegradability, biocompatibility, aqueous solubility, viscoelasticity, and non-immunogenicity. PMID: 30084325 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Design - August 7, 2018 Category: Drugs & Pharmacology Authors: Shah K, Chawla S, Agrawal A, Reddy G, Maheshwari R, Kalia K, Tekade RK Tags: Curr Pharm Des Source Type: research

TARGETED LIPOSOMES FOR siRNA DELIVERY TO CANCER.
CONCLUSION: siRNA delivery mediated by functionalized liposomes is promising, but clinical trials need to be conducted. PMID: 30084323 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Design - August 7, 2018 Category: Drugs & Pharmacology Authors: Eloy JO, Petrilli R, Raspantini GL, Lee RJ Tags: Curr Pharm Des Source Type: research

Reversible Covalent-Crosslinked Polycations with Enhanced Stability and ATP-Responsive Behavior for Improved siRNA Delivery.
In this study, we proposed a new strategy of reversible-covalent crosslinking to enhance stability in circulation and enable stimuli-disassembly of polyplexes in tumor cells. Here, 25k polyethyleneimine (PEI) was chosen as model polycations for verifying the hypothesis. HA-PEI conjugation was formed by the crosslinking of adenosine triphosphate grafted HA (HA-ATP) with phenylboronic acid grafted PEI (PEI-PBA), via the chemical reaction between PBA and ATP. Compared with non-covalent polyplex by electrostatic interaction (HA/PEI), HA-PEI exhibited much better colloidal stability and serum stability. The covered HA-ATP layer...
Source: Biomacromolecules - August 6, 2018 Category: Biochemistry Authors: Zhou Z, Zhang M, Liu Y, Li C, Zhang Q, Oupicky D, Sun M Tags: Biomacromolecules Source Type: research

Development of an Alcohol Dilution-Lyophilization Method for Preparing Lipid Nanoparticles Containing Encapsulated siRNA.
In this study, we report on the development of an "alcohol dilution-lyophilization method" for preparing siRNA-encapsulating LNPs. This method involves the use of a freeze-drying (lyophilization) method to remove the residual alcohol and to simultaneously concentrate the preparation. At first, the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used were optimized from the point of view of particle stabilization. A combination of sucrose and 1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000) was found to have the most efficient cryoprotective activity for the LNPs. T...
Source: Biological and Pharmaceutical Bulletin - August 3, 2018 Category: Drugs & Pharmacology Authors: Shirane D, Tanaka H, Nakai Y, Yoshioka H, Akita H Tags: Biol Pharm Bull Source Type: research

GSE110567 RNA-seq analysis of equine tenocytes exposed to siRNA targeting scleraxis (Scx) to identify novel targets of Scx-mediated mechanotransduction
Contributors : Anne E Nichols ; Robert E Settlage ; Stephen R Werre ; Linda A DahlgrenSeries Type : Expression profiling by high throughput sequencingOrganism : Equus caballusEquine tenocytes were exposed to an siRNA targeting the transcription factor scleraxis (Scx) or a scramble control and maintained under cyclic strain. The resulting transcriptomes were then used for RNA-seq analysis. The goal of this study was to identify novel ways in which Scx facilitates mechanotransduction in tenocytes in order to better understand tenocyte biology.
Source: GEO: Gene Expression Omnibus - August 2, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Equus caballus Source Type: research