Query: siRNA

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Total 17427 results found since Jan 2013.

GSE121627 Analysis of HPV16 E2 host gene expression using TERT immortalized keratinocytes (NOKs) cell lines and RNA-sequencing
Contributors : Micael R Evans ; Claire D James ; Molly L Bristol ; Tara J Nulton ; Xu Wang ; Namsimar Kaur ; Elizabeth A White ; Brad Windle ; Iain M MorganSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensHPV must reprogram host gene expression to promote infection, and HPV proteins E6 and E7 contribute to this via targeting of cellular transcription factors including p53 and pRb respectively. The HPV16 E2 protein regulated host gene expression in U2OS cells and in this study we extend these observations into NOKs that are capable of suporting late stages of the HPV16 life cycle. We ob...
Source: GEO: Gene Expression Omnibus - October 23, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE121584 Steroid Receptor Coactivator-2 Regulated Transcriptome in Human Endometrial Stromal Cells
Contributors : John P Lydon ; Maria M SzwarcSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRNA-sequencing of mRNA isolated from in vitro decidualizaing human endometrial stromal cells with or without siRNA-mediated knockdown of steroid receptor coactivator-2/nuclear receptor coactivator 2 (SRC-2/NCOA2)
Source: GEO: Gene Expression Omnibus - October 23, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Methylenetetrahydrofolate Dehydrogenase 1 Silencing Expedites the Apoptosis of Non-Small Cell Lung Cancer Cells via Modulating DNA Methylation.
CONCLUSIONS Collectively, our results proved that MTHFD1 silencing obviously reduced the proliferation and enhanced the apoptosis of NSCLC via suppressing DNA methylation. PMID: 30343310 [PubMed - in process]
Source: Medical Science Monitor - October 22, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Ectopic lipid accumulation: potential role in tubular injury and inflammation in diabetic kidney disease.
Emerging studies suggest that lipid accumulates in the kidneys during diabetic kidney disease (DKD). However, the correlation between ectopic lipid accumulation with tubular damage has not been thoroughly elucidated to date. Using Oil Red staining, lipid accumulation was observed in the kidneys of type 2 DKD patients (class II-III) and db/db mice compared to the control and was predominantly located in the proximal tubular compartment. Immunohistochemistry staining showed that the intensity of adipose differentiation related protein (ADRP) and sterol-regulatory element binding protein-1 (SREBP-1) was clearly upregulated, w...
Source: Clinical Science - October 22, 2018 Category: Biomedical Science Authors: Yang, W., Luo, Y., Yang, S., Zeng, M., Zhang, S., Liu, J., Han, Y., Liu, Y., Zhu, X., Wu, H., Liu, F., Sun, L., Xiao, L. Tags: PublishAheadOfPrint Source Type: research

Smart nanoparticles assembled by endogenous molecules for siRNA delivery and Cancer therapy via CD44 and EGFR dual-targeting
Publication date: Available online 21 October 2018Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Yaoyao Liang, Jiahui Peng, Ning Li, Cynthia Yu-Wai-Man, Qian Wang, Yuhong Xu, Hongxia Wang, Aristides D Tagalakis, Zixiu DuAbstractWe developed an anticancer siRNA delivery system (named HLPR) through modular assembly of endogenous molecules. The structure of HLPR was a tightly condensed siRNA-peptide inner core in turn surrounded by the disordered lipid layer and thin HA coating from which the EGFR-targeted amino acid sequences of IVNQPTYGYWHY partially protrude outside of cell surfaces. Both HA and IVNQP...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - October 21, 2018 Category: Nanotechnology Source Type: research

Knockdown of RTN1-C attenuates traumatic neuronal injury through regulating intracellular Ca2+ homeostasis
Publication date: Available online 21 October 2018Source: Neurochemistry InternationalAuthor(s): Xiao-xuan Fan, Yu-ying Hao, Shi-wen Guo, Xiao-ping Zhao, Yi Xiang, Fei-xue Feng, Ge-ting Liang, Yu-wei DongAbstractReticulons (RTNs) are a family of membrane-bound proteins that are dominantly localized to the endoplasmic reticulum (ER) membrane. RTN1-C is one member of RTNs abundantly expressed in the brain and has been shown to mediate neuronal injury in cerebral ischemia models. In the present study, we investigated the role of RTN1-C in an in vitro brain trauma model mimicked by traumatic neuronal injury (TNI) in primary cu...
Source: Neurochemistry International - October 21, 2018 Category: Neuroscience Source Type: research

Protective effects ROS up-regulation on premature ovarian failure by suppressing ROS-TERT signal pathway.
CONCLUSIONS: ROS up-regulation inhibits TERT expression, suppresses TERT activity and facilitates POF. The ROS-TERT pathway may work as the target for treating POF. PMID: 30338789 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - October 21, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

IGF-1 regulates Ang II and VEGF signaling pathways in retinal neovascularization.
CONCLUSIONS: The occurrence of retinal neovascularization is related to the reduction of IGF-1 expression and the increase of Ang II and VEGF. PMID: 30338783 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - October 21, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Silencing BRIT1 Facilitates the Abilities of Invasiveness and Migration in Trophoblast Cells.
CONCLUSIONS BRIT1 silencing accelerated the invasion and migration of TC and activated the Wnt/β-catenin pathway. Our results may provide new insights for finding new molecular targets to cure disease caused by insufficient invasion of TC. PMID: 30337515 [PubMed - in process]
Source: Medical Science Monitor - October 21, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Hepatocyte growth factor-induced mesenchymal-epithelial transition factor activation leads to insulin-like growth factor 1 receptor inhibitor unresponsiveness in gastric cancer cells.
In conclusion, HGF-induced MET activation may represent a novel mechanism conferring unresponsiveness to IGF-1R-targeted agents in GC, and inhibition of MET may improve the efficacy of IGF-1R inhibitors. PMID: 30333869 [PubMed]
Source: Oncology Letters - October 21, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Leptin and its receptor in glucose metabolism of T-cell lymphoma.
In conclusion, these results suggested that leptin serves a critical role in TCL glucose uptake via the ObR. PMID: 30333864 [PubMed]
Source: Oncology Letters - October 21, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Coated microneedles mediated intradermal delivery of octaarginine/BRAF siRNA nanocomplexes for anti-melanoma treatment
In this study, we for the first time developed a siBraf delivery system based on cell penetrating peptide octaarginine (R8) nanocomplexes combined with coated microneedles (MNs), i.e. R8/siBraf coated MNs, for targeted anti–melanoma treatment. The R8/siBraf nanocomplexes were optimized based on the internalization of siBraf by A375 cells. In vitro A375 cell experiments presented that R8/siBraf can enhance siBraf transfection, silence BRAF gene, and inhibit tumor cells growth, comparable to polyethylenimine (PEI)/siBraf. R8/siBraf coated MNs can effectively deliver R8/siBraf into the disease site. In vivo anti-melanoma ex...
Source: International Journal of Pharmaceutics - October 20, 2018 Category: Drugs & Pharmacology Source Type: research

Estrogen signaling increases nuclear receptor subfamily 4 group A member 1 expression and energy production in skeletal muscle cells.
Authors: Nagai S, Ikeda K, Horie-Inoue K, Takeda S, Inoue S Abstract Estrogen deficiency has been known to associate with musculoskeletal diseases in women, based on the clinical observations of frequent susceptibility to osteoporosis and sarcopenia among postmenopausal women. In skeletal muscles, estrogen has been assumed to play physiological roles in maintaining muscle mass and strength, although its precise molecular mechanism remains to be elucidated. We have previously shown that estrogen regulates energy metabolism through the downregulation of mitochondrial uncoupling protein 3 (UCP3) in skeletal muscles, w...
Source: Endocrine Journal - October 20, 2018 Category: Endocrinology Tags: Endocr J Source Type: research

IRS-2 deubiquitination by USP9X maintains anchorage-independent cell growth via Erk1/2 activation in prostate carcinoma cell line.
Authors: Furuta H, Yoshihara H, Fukushima T, Yoneyama Y, Ito A, Worrall C, Girnita A, Girnita L, Yoshida M, Asano T, Komada M, Kataoka N, Chida K, Hakuno F, Takahashi SI Abstract Insulin-like growth factors (IGFs) have been shown to induce proliferation of many types of cells. Insulin receptor substrates (IRSs) are major targets of IGF-I receptor (IGF-IR) tyrosine kinase activated by IGFs, and are known to play important roles in the activation of downstream signaling pathways, such as the Erk1/2 pathway. Dysregulation of IGF signaling represents a central tumor promoting principle in human carcinogenesis. Prostate...
Source: Oncotarget - October 20, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

GSE121536 TFAP2C signalling in human fibroblasts
Contributors : Danielle Park ; Erik Sahai ; Stefan BoeingSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensOur work has shown that the transcription factor TFAP2C is upregulated in fibroblasts that generate an anisotrophic extracellular matrix. To determine what is acting downstream of TFAP2C we compared fibroblasts that had been mock transfected, or treated with a non-targeting siRNA control or siRNA against TFAP2C.
Source: GEO: Gene Expression Omnibus - October 20, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Endoplasmic Reticulum Stress Induces MUC5AC and MUC5B Expression in Human Nasal Airway Epithelial Cells.
Conclusion: These results demonstrate that ER stress plays an important role in the regulation of MUC5AC and MUC5B via the activations of XBP-1, CHOP, and ATF6 in human nasal airway epithelial cells. PMID: 30336657 [PubMed - as supplied by publisher]
Source: Clinical and Experimental Otorhinolaryngology - October 20, 2018 Category: ENT & OMF Tags: Clin Exp Otorhinolaryngol Source Type: research

Tumor Susceptibility Gene 101 Mediates Anoikis Resistance of Metastatic Thyroid Cancer Cells
Conclusion: To our knowledge, this is the first study that implicates the importance of TSG101 in anoikis resistance of thyroid cancer.
Source: Cancer Genomics and Proteomics - October 20, 2018 Category: Cancer & Oncology Authors: SAHARAT, K., LIRDPRAPAMONGKOL, K., CHOKCHAICHAMNANKIT, D., SRISOMSAP, C., SVASTI, J., PARICHARTTANAKUL, N. M. Tags: Article Source Type: research

Transforming Growth Factor β1 (TGF-β1)-Stimulated Integrin-Linked Kinase (ILK) Regulates Migration and Epithelial-Mesenchymal Transition (EMT) of Human Lens Epithelial Cells via Nuclear Factor κB (NF-κB).
CONCLUSIONS TGF-β-stimulated ILK regulates the migration and EMT of human LECs via NF-κB. PMID: 30332398 [PubMed - in process]
Source: Medical Science Monitor - October 19, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Role of Spata34 in cell proliferation and its expression pattern in postnatal development of rat testis.
This study intends to examine the expression profiles of Spata34 in postnatal rat testis, and explore its potential roles in cell proliferation in vitro. We found that the mRNA and protein expression levels of Spata34 were developmentally upregulated in rat testes during the early 1-7 postnatal weeks using real-time polymerase chain reaction and western blotting. Immunohistochemical results indicated that Spata34 protein was mainly detected in the nuclear and cytoplasm of spermatocytes and round spermatids. The possible function of Spata34 in cellular proliferation was analyzed using cell counting kit, colony formation and...
Source: Molecular Biology Reports - October 19, 2018 Category: Molecular Biology Authors: Nie D, Li H, Liu Y, Liao Z Tags: Mol Biol Rep Source Type: research

Upregulation of AMPK by 4-O-methylascochlorin promotes autophagy via the HIF-1 α expression.
Upregulation of AMPK by 4-O-methylascochlorin promotes autophagy via the HIF-1α expression. J Cell Mol Med. 2018 Oct 19;: Authors: Seok JY, Jeong YJ, Hwang SK, Kim CH, Magae J, Chang YC Abstract 4-O-methylascochlorin (MAC) is a derivative of ascochlorin, a prenyl-phenol compound antibiotic isolated from the fungus Ascochyta viciae. MAC induces caspase/poly (ADP-ribose) polymerase-mediated apoptosis in leukemia cells. However, the effects of MAC on autophagy in cancer cells and the underlying molecular mechanisms remain unknown. Here, we show that MAC induces autophagy in lung cancer cells. MAC signif...
Source: J Cell Mol Med - October 19, 2018 Category: Molecular Biology Authors: Seok JY, Jeong YJ, Hwang SK, Kim CH, Magae J, Chang YC Tags: J Cell Mol Med Source Type: research

HMGB1 deficiency reduces H2 O2 -induced oxidative damage in human melanocytes via the Nrf2 pathway.
In this study, we investigated the role of HMGB1 from melanocytes in the response to oxidative stress and the mechanism involved. We showed that HMGB1 is expressed by primary normal human epidermal melanocytes (NHEMs). H2 O2 treatment increased cytoplasmic translocation and extracellular release of HMGB1. HMGB1 knockdown by small interfering RNA (siRNA) led to decreased apoptosis of NHEMs. HMGB1 inhibition enhanced the expression of Nrf2 and its target genes. The expression of Nrf2 and its downstream antioxidant genes was downregulated after the supernatant of H2 O2 -treated NHEMs was added to HMGB1-deficient cells. HMGB1 ...
Source: J Cell Mol Med - October 19, 2018 Category: Molecular Biology Authors: Mou K, Liu W, Miao Y, Cao F, Li P Tags: J Cell Mol Med Source Type: research

Exosomal KLF3-AS1 from hMSCs promoted cartilage repair and chondrocyte proliferation in osteoarthritis.
This study was designed to explore whether exosomal lncRNA-KLF3-AS1 derived from human mesenchymal stem cells (hMSCs) can serve as a positive treatment for osteoarthritis (OA). hMSCs and MSC-derived exosomes (MSC-exo) were prepared for morphological observation and identification by transmission electron microscopy (TEM) and flow cytometry. IL-1β-induced OA chondrocytes and collagenase-induced rat model of OA were established for the further experiments. Lentivirus mediated siRNA targeting KLF3-AS1 was transfected into MSCs for silencing KLF3-AS1. The real-time quantitative PCR (qRT-PCR) and western blotting analysis were...
Source: The Biochemical Journal - October 19, 2018 Category: Biochemistry Authors: Liu Y, Zou R, Wang Z, Wen C, Zhang F, Lin F Tags: Biochem J Source Type: research

Role  of Annexin A2 isoform2 on the aggregative growth of dermal papillae cells.
Role of Annexin A2 isoform2 on the aggregative growth of dermal papillae cells. Biosci Rep. 2018 Oct 19;: Authors: Gu J, Ma Y, Yang L, Wang F, Lei C, Zhai J, Xia R Abstract Dermal papilla signals the follicular epithelial cells to prolong the hair growth process. Human Annexin A2 was preliminarily identified by 2-DE, MALDI-TOF-MS and database searching. The aim of the study was to explore the role of Annexin A2 in dermal papilla cells(DPC). RT-PCR and Western blot were adopted to detect the expression of Annexin A2. And siRNA technique was used to suppress the expression of Annexin A2. Over-expre...
Source: Bioscience Reports - October 19, 2018 Category: Biomedical Science Authors: Gu J, Ma Y, Yang L, Wang F, Lei C, Zhai J, Xia R Tags: Biosci Rep Source Type: research

The ORMDL3 Asthma Gene Regulates ICAM1 and has Multiple Effects on Cellular Inflammation.
CONCLUSIONS: The results show ORMDL3 has pleiotropic effects during cellular inflammation, consistent with its substantial genetic influence on childhood asthma. Actions on ICAM1 provide a mechanism for the locus to confer susceptibility to HRV-induced asthma. PMID: 30339462 [PubMed - as supplied by publisher]
Source: American Journal of Respiratory and Critical Care Medicine - October 19, 2018 Category: Respiratory Medicine Authors: Zhang Y, Willis-Owen SAG, Spiegel S, Lloyd CM, Moffatt MF, Cookson WOCM Tags: Am J Respir Crit Care Med Source Type: research

Pretreatment TACC3 expression in locally advanced rectal cancer decreases the response to neoadjuvant chemoradiotherapy.
In this study, we investigated whether TACC3 could serve as a biomarker predictive of the efficacy of chemoradiotherapy. In all, 152 rectal cancer patients with tumor tissue collected at biopsy and set aside before treatment were enrolled in this study. All patients received chemoradiotherapy and surgical resection. Immunohistochemically detected tumoral TACC3 expression significantly decreased sensitivity to chemoradiotherapy [risk ratio (RR) = 2.236, 95% confidence interval (CI): 1.447-3.456; P = 0.001] and thus the pathological complete response rate (P = 0.001). TACC3 knockdown using specific siRNA enhanced radiotherap...
Source: Aging - October 19, 2018 Category: Biomedical Science Authors: Ma WJ, Gu YK, Peng JH, Wang XC, Yue X, Pan ZZ, Chen G, Xu HN, Zhou ZG, Zhang RX Tags: Aging (Albany NY) Source Type: research

Transgelin as a potential target in the reversibility of pulmonary arterial hypertension secondary to congenital heart disease.
CONCLUSIONS: Transgelin may be a potential target in the development of irreversible CHD-PAH through inducing PASMC phenotype change, proliferation, migration and reducing cell apoptosis. PMID: 30338626 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - October 18, 2018 Category: Molecular Biology Authors: Huang L, Li L, Yang T, Li W, Song L, Meng X, Gu Q, Xiong C, He J Tags: J Cell Mol Med Source Type: research

Worm ‐Like Biomimetic Nanoerythrocyte Carrying siRNA for Melanoma Gene Therapy
Small, EarlyView.
Source: Small - October 18, 2018 Category: Nanotechnology Authors: Yanming Wang, Xin Ji, Miaoliang Ruan, Wen Liu, Rongguang Song, Jian Dai, Wei Xue Source Type: research

Aurora kinase B siRNA-loaded lactoferrin nanoparticles potentiate the efficacy of temozolomide in treating glioblastoma
Nanomedicine, Ahead of Print.
Source: Future Medicine: Nanomedicine - October 18, 2018 Category: Nanotechnology Authors: Sonali Kumari Dwaipayan Bhattacharya Nandini Rangaraj Sumana Chakarvarty Anand K Kondapi Nalam M Rao Source Type: research

Th1 Cytokines, TNF α and IFNγ, Promote Corticosteroid Resistance in Developing Human Airway Smooth Muscle.
Th1 Cytokines, TNFα and IFNγ, Promote Corticosteroid Resistance in Developing Human Airway Smooth Muscle. Am J Physiol Lung Cell Mol Physiol. 2018 Oct 18;: Authors: Britt RD, Thompson MA, Sasse SK, Pabelick CM, Gerber AN, Prakash YS Abstract Corticosteroids are commonly used to manage wheezing and asthma in pediatric populations. Although corticosteroids are effective in alleviating airway diseases, some children with more moderate-severe asthma phenotypes show corticosteroid (CS) resistance and exhibit significant airflow obstruction, persistent inflammation, and more frequent exacerbations. Previo...
Source: Am J Physiol Lung Ce... - October 18, 2018 Category: Respiratory Medicine Authors: Britt RD, Thompson MA, Sasse SK, Pabelick CM, Gerber AN, Prakash YS Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Aurora kinase B siRNA-loaded lactoferrin nanoparticles potentiate the efficacy of temozolomide in treating glioblastoma
Nanomedicine, Ahead of Print.
Source: Nanomedicine - October 18, 2018 Category: Nanotechnology Authors: Sonali Kumari Dwaipayan Bhattacharya Nandini Rangaraj Sumana Chakarvarty Anand K Kondapi Nalam M Rao Source Type: research

Translocation Associated Membrane Protein 1 Contributes to Chronic Constriction Injury-Induced Neuropathic Pain in the Dorsal Root Ganglion and Spinal Cord in Rats
AbstractNeuropathic pain is a severe debilitating state caused by injury or dysfunction of somatosensory nervous system, and the clinical treatment is still challenging. Translocation associated membrane protein 1 (TRAM1), an adapter protein, participates in a variety of transduction pathways and mediates the biological functions such as cell proliferation, activation, and differentiation. However, whether TRAM1 is involved in the pathogenesis of neuropathic pain is still unclear. In our study, we reported the role of TRAM1 in the maintenance of neuropathic pain induced by chronic constriction injury (CCI) on rats. By west...
Source: Journal of Molecular Neuroscience - October 18, 2018 Category: Neuroscience Source Type: research

Worm ‐Like Biomimetic Nanoerythrocyte Carrying siRNA for Melanoma Gene Therapy
Small, EarlyView.
Source: Small - October 18, 2018 Category: Nanotechnology Authors: Yanming Wang, Xin Ji, Miaoliang Ruan, Wen Liu, Rongguang Song, Jian Dai, Wei Xue Source Type: research

Lentivirus-mediated siRNA knockdown of SPHK1 inhibits proliferation and tumorigenesis of neuroblastoma
Source: OncoTargets and Therapy - October 17, 2018 Category: Cancer & Oncology Tags: OncoTargets and Therapy Source Type: research

Elucidating the novel BRCA1 function as a non-genomic metabolic restraint in ER-positive breast cancer cell lines.
Authors: Koobotse M, Holly J, Perks C Abstract Within populations carrying the same genetic predisposition, the penetrance of BRCA1 mutations has increased over time. Although linked to changes in lifestyle factors associated with energy metabolism, these observations cannot be explained by the established role of BRCA1 in DNA repair alone. We manipulated BRCA1 expression using tetracycline in the UBR60-bcl2 cell line (which has an inducible, tetracycline-regulated BRCA1 expression) and siRNA in oestrogen receptor(ER)-positive MCF7 and T47D breast cancer cells. Cellular responses to BRCA1 silencing and IGF-I action...
Source: Oncotarget - October 17, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Comparative proteomic analysis of cat eye syndrome critical region protein 1- function in tumor-associated macrophages and immune response regulation of glial tumors.
Conclusion: This study reports the molecular pathways and key molecules that are mediated by CECR1 function in THP- 1-derived MQs and TAMs in glioma. Methods: PMA-treated THP-1 cells (MQs) were siRNA transfected for CECR1 in vitro, with or without stimulation of the primary glioma cell line U87. Lysates were analyzed by (nano)LC-MS. Significant altered protein levels were identified (P < 0.05), followed by pathway annotation. PMID: 30323894 [PubMed]
Source: Oncotarget - October 17, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Silencing of the nucleocytoplasmic shuttling protein karyopherin a2 promotes cell-cycle arrest and apoptosis in glioblastoma multiforme.
This study aims to further elucidate this role of KPNA2 in in vitro GBM models. From four different tested GBM cell lines, the U87MG showed the highest proliferation, low adherence and outgrowth in 3D clusters as well as the highest expression of KPNA2, all features conferring greater malignant behaviour. Silencing of KPNA2 via siRNA interference in those cells significantly decreased their proliferative capacity (p = 0.001). We further observed both a significant cell cycle phase arrest (p = 0.040) and the promoting of cellular apoptosis (p = 0.016) as well as a strong trend (p = 0.062) for an inhibition of nuclear import...
Source: Oncotarget - October 17, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Tumor-Associated Fibronectin Targeted Liposomal Nanoplatform for Cyclophilin A siRNA Delivery and Targeted Malignant Glioblastoma Therapy
Phei Er Saw, Ao Zhang, Yan Nie, Lei Zhang, Yingjie Xu, Xiaoding Xu
Source: Frontiers in Pharmacology - October 17, 2018 Category: Drugs & Pharmacology Source Type: research

Dihomo-γ-Linolenic Acid Inhibits Growth of Xenograft Tumors in Mice Bearing Human Pancreatic Cancer Cells (BxPC-3) Transfected with Delta-5-Desaturase shRNA
In this study, we have further investigated the anti-tumor effects of D5D-knockdown and the resulting intensified COX-2-catalyzed DGLA peroxidation in subcutaneous xenograft tumors. Four-week old female nude mice (Jackson Laboratory, J:Nu-007850) were injected with human pancreatic cancer cell line BxPC-3 or its D5D knockdown counterpart (via shRNA), followed by 4-week treatments of: vehicle control, DGLA supplementation (8 mg/mouse, twice a week), gemcitabine (30 mg/kg, twice a week), and a combination of DGLA and gemcitabine. In D5D-knockdown tumors, DGLA supplementation promoted 8-HOA formation to a threshold ...
Source: Redox Biology - October 17, 2018 Category: Biology Source Type: research

Resveratrol attenuates high fat diet-induced mouse cardiomyopathy through upregulation of estrogen related receptor- α.
Resveratrol attenuates high fat diet-induced mouse cardiomyopathy through upregulation of estrogen related receptor-α. Eur J Pharmacol. 2018 Oct 17;: Authors: Lu Y, Lu X, Wang L, Yang W Abstract Resveratrol reportedly promotes the improvement of cardiac dysfunction and other cardiovascular diseases. Studies demonstrate resveratrol exhibits a set of benefits, including anti-oxidative property, anti-apoptosis and anti-inflammation. However, the molecular mediators of resveratrol-induced cardiac benefits are still not fully disclosed. Present study aims to investigate whether estrogen-related receptor (...
Source: European Journal of Pharmacology - October 17, 2018 Category: Drugs & Pharmacology Authors: Lu Y, Lu X, Wang L, Yang W Tags: Eur J Pharmacol Source Type: research

LncRNA AWPPH promotes the growth of triple-negative breast cancer by up-regulating frizzled homolog 7 (FZD7).
CONCLUSIONS: We conclude that LncRNA AWPPH may promote the growth of triple-negative breast cancer by down-regulating FZD7. PMID: 30333256 [PubMed - as supplied by publisher]
Source: Bioscience Reports - October 17, 2018 Category: Biomedical Science Authors: Wang K, Li X, Song C, Li M Tags: Biosci Rep Source Type: research

GCN5 inhibition prevents IL-6-induced prostate cancer metastases by inactivating Egr-1.
In conclusion, these data demonstrated the negative effect of upregulated GCN5 in IL-6-induced metastasis and EMT in PCa cells through PI3K/PTEN/Akt sigaling pathway down-regulating Egr-1 expression. PMID: 30333255 [PubMed - as supplied by publisher]
Source: Bioscience Reports - October 17, 2018 Category: Biomedical Science Authors: Shao G, Liu Y, Ma T, Zhang L, Yuan M, Zhao S Tags: Biosci Rep Source Type: research

Systematic identification of celastrol-binding proteins reveals that Shoc2 is inhibited by celastrol.
This study aimed to use in vitro and in vivo methods to clarify the anti-tumor effect of Celastrol and use protein microarrays to explore its mechanisms. We demonstrated that Celastrol effectively inhibited SW480 CRC cell proliferation. Two weeks of Celastrol gavage significantly inhibited the growth of xenografts in nude mice. A total of 69 candidate proteins were identified in the protein microarray experiment, including the most highly enriched protein Shoc2, which is a scaffold protein that modulates cell motility and metastasis through the ERK pathway. Celastrol significantly inhibited ERK1/2 phosphorylation in cell l...
Source: Bioscience Reports - October 17, 2018 Category: Biomedical Science Authors: Huang XP, Chen JK, Xu W, Dong YF, Yan L, Zhang XF, Pan YM, Chang WJ, Zhu JB Tags: Biosci Rep Source Type: research

Staphylococcus epidermidis-induced Interleukin-1 Beta and Human Beta-defensin-2 Expression in Human Keratinocytes is Regulated by the Host Molecule A20 (TNFAIP3).
We report here that S. epidermidis induces expression of the host regulator protein A20 in human keratinocytes, thereby controlling expression and release of interleukin-1 beta. siRNA-mediated knockdown of A20 expression strongly enhanced the induction of interleukin-1 beta gene expression and protein release in keratinocytes stimulated with S. epidermidis. Furthermore, siRNA-mediated knockdown of A20 resulted in enhanced gene expression and secretion of the antimicrobial peptide human beta-defensin-2 in keratinocytes facing S. epidermidis. Mechanistically, A20 negatively controlled S. epidermidis-induced activation of the...
Source: Acta Dermato-Venereologica - October 17, 2018 Category: Dermatology Authors: Simanski M, Erkens AS, Rademacher F, Harder J Tags: Acta Derm Venereol Source Type: research

PKA regulates HMGB1 through activation of IGFBP-3 and SIRT1 in human retinal endothelial cells cultured in high glucose
ConclusionsPKA requires active IGFBP-3 and SIRT1 to inhibit HMGB1 inflammatory actions in the retina vasculature. Activation of these pathways may offer new targets for therapy development.
Source: Inflammation Research - October 17, 2018 Category: Research Source Type: research

Cyclic peptide-based nanostructures as efficient siRNA carriers.
Authors: Panigrahi B, Singh RK, Mishra S, Mandal D Abstract RNA interference shows a great strategy for biological studies; however, delivering of small interfering RNA (siRNA) remains challenging. Although several delivery vehicles, including cell-penetrating peptides, have been developed, their implementation is often restricted because of their endosomal entrapment. Herein, we report the formation of self-assembled nanostructures from rationally designed cyclic peptides and explore them for efficient delivery of functional biomacromolecules such as siRNA into mammalian cells. The newly obtained soft materials ma...
Source: Artificial Cells, Nanomedicine and Biotechnology - October 14, 2018 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Expression of retinoic acid signaling components ADH7 and ALDH1A1 is reduced in aniridia limbal epithelial cells and a siRNA primary cell based aniridia model.
Abstract PAX6-related Aniridia is a sight-threatening disease involving progression of secondary glaucoma and aniridia related keratopathy (ARK). Change or loss of limbal epithelial progenitors causes epithelial surface defects. We analyzed the effect of PAX6 on mRNA expression changes with a two-step approach, as follows. First, we sequenced mRNA from limbal epithelial cells isolated from controls and aniridia patients. Second, we confirmed the bioinformatics and literature-based result list for a small interfering RNA (siRNA)-based primary aniridia cell model (PAX6 knockdown). With this approach, we expected tha...
Source: Experimental Eye Research - October 4, 2018 Category: Opthalmology Authors: Latta L, Nordström K, Stachon T, Langenbucher A, Fries FN, Szentmáry N, Seitz B, Käsmann-Kellner B Tags: Exp Eye Res Source Type: research

Dual-modified cationic liposomes loaded with paclitaxel and survivin siRNA for targeted imaging and therapy of cancer stem cells in brain glioma.
In conclusion, prepared DP-CLPs-PTX-siRNA nanocomplex selectively induced CD133+ glioma stem cell apoptosis in vitro and in vivo exhibits great potential for targeted imaging and therapy of brain glioma stem cells. PMID: 30269613 [PubMed - in process]
Source: Drug Delivery - October 3, 2018 Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research

GSE115268 human pulmonary artery (endothelial and smoorh muscle) cells : control vs ARSB-siRNA transfected
Contributors : Anna Lalik ; Adam Golda ; Sebastian StudentSeries Type : Expression profiling by arrayOrganism : Homo sapiensTranscriptional profiling of human pulmonary artery endothelial (HAPEC) and smooth muscle (PASM) cells comparing control untreated cells with cells transfected with ARSB-siRNA. Goal was to determine the effects of silenced of arylsufatase B on global gene expression in HAPEC and PASM cells.
Source: GEO: Gene Expression Omnibus - October 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Current Transport Systems and Clinical Applications for Small Interfering RNA (siRNA) Drugs
AbstractSmall interfering RNAs (siRNAs) are an attractive new agent with potential as a therapeutic tool because of its ability to inhibit specific genes for many conditions, including viral infections and cancers. However, despite this potential, many challenges remain, including off-target effects, difficulties with delivery, immune responses, and toxicity. Traditional genetic vectors do not guarantee that siRNAs will silence genes in vivo. Rational design strategies, such as chemical modification, viral vectors, and non-viral vectors, including cationic liposomes, polymers, nanocarriers, and bioconjugated siRNAs, provid...
Source: Molecular Diagnosis and Therapy - October 1, 2018 Category: Molecular Biology Source Type: research

Manifold roles of β-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9.
Abstract G protein-coupled receptors (GPCRs) use diverse mechanisms to regulate the mitogen-activated protein kinases ERK1/2. β-Arrestins (βArr1/2) are ubiquitous inhibitors of G protein signaling, promoting GPCR desensitization and internalization and serving as scaffolds for ERK1/2 activation. Studies using CRISPR/Cas9 to delete βArr1/2 and G proteins have cast doubt on the role of β-arrestins in activating specific pools of ERK1/2. We compared the effects of siRNA-mediated knockdown of βArr1/2 and reconstitution with βArr1/2 in three different parental and CRISPR-derived βArr1/2 knockout HEK293 cell pair...
Source: Science Signaling - September 25, 2018 Category: Biomedical Science Authors: Luttrell LM, Wang J, Plouffe B, Smith JS, Yamani L, Kaur S, Jean-Charles PY, Gauthier C, Lee MH, Pani B, Kim J, Ahn S, Rajagopal S, Reiter E, Bouvier M, Shenoy SK, Laporte SA, Rockman HA, Lefkowitz RJ Tags: Sci Signal Source Type: research