Recent advances in the use of Trichoderma-containing multicomponent microbial inoculants for pathogen control and plant growth promotion
AbstractChemical pesticides and fertilizers are used in agricultural production worldwide to prevent damage from plant pathogenic microorganisms, insects, and nematodes, to minimize crop losses and to preserve crop quality. However, the use of chemical pesticides and fertilizers can severely pollute soil, water, and air, posing risks to the environment and human health. Consequently, developing new, alternative, environment-friendly microbial soil treatment interventions for plant protection and crop yield increase has become indispensable. Members of the filamentous fungal genusTrichoderma (Ascomycota, Sordariomycetes, Hy...
Source: World Journal of Microbiology and Biotechnology - April 13, 2024 Category: Microbiology Source Type: research

Influence of flavonoids from Sedum aizoon L. on mitochondrial function of Rhizopus nigricans in strawberry
In this study, the minimum inhibitory concentration (MIC) of FSAL onR. nigricans and cell apoptosis tests were studied to explore the inhibitory effect of FSAL onR. nigricans. The effects of FSAL on mitochondria ofR. nigricans were investigated through the changes of mitochondrial permeability transition pore(mPTP), mitochondrial membrane potential(MMP), Ca2+ content, H2O2 content, cytochromec (Cyt c) content, the related enzyme activity and related genes of mitochondria. The results showed that the MIC of FSAL onR. nigricans was 1.800  mg/mL, with the addition of FSAL (1.800 mg/mL), the mPTP openness ofR. nigricans incr...
Source: World Journal of Microbiology and Biotechnology - April 13, 2024 Category: Microbiology Source Type: research

Advances in biotin biosynthesis and biotechnological production in microorganisms
AbstractBiotin, also known as vitamin H or B7, acts as a crucial cofactor in the central metabolism processes of fatty acids, amino acids, and carbohydrates. Biotin has important applications in food additives, biomedicine, and other fields. While the ability to synthesize biotinde novo is confined to microorganisms and plants, humans and animals require substantial daily intake, primarily through dietary sources and intestinal microflora. Currently, chemical synthesis stands as the primary method for commercial biotin production, although microbial biotin production offers an environmentally sustainable alternative with p...
Source: World Journal of Microbiology and Biotechnology - April 13, 2024 Category: Microbiology Source Type: research

Microbial chassis design and engineering for production of gamma-aminobutyric acid
AbstractGamma-aminobutyric acid (GABA) is a non-protein amino acid which is widely applied in agriculture and pharmaceutical additive industries. GABA is synthesized from glutamate through irreversible α-decarboxylation by glutamate decarboxylase. Recently, microbial synthesis has become an inevitable trend to produce GABA due to its sustainable characteristics. Therefore, reasonable microbial platform design and metabolic engineering strategies for improving production of GABA are arousing a con siderable attraction. The strategies concentrate on microbial platform optimization, fermentation process optimization, rationa...
Source: World Journal of Microbiology and Biotechnology - April 12, 2024 Category: Microbiology Source Type: research

Strategies for the efficient biosynthesis of β-carotene through microbial fermentation
Abstractβ-Carotene is an orange fat-soluble compound, which has been widely used in fields such as food, medicine and cosmetics owing to its anticancer, antioxidant and cardiovascular disease prevention properties. Currently, natural β-carotene is mainly extracted from plants and algae, which cannot meet the growing market demand, while chemical synthesis of β-carotene cannot satisfy the pursuit for natural products of consumers. The β-carotene production through microbial fermentation has become a promising alternative owing to its high efficiency and environmental friendliness. With the rapid de velopment of syntheti...
Source: World Journal of Microbiology and Biotechnology - April 12, 2024 Category: Microbiology Source Type: research

Synthesis of bio-stabilized silver nanoparticles using Roccella montagnei, their anticandidal capacities & amp; potential to inhibit the virulence factors in fluconazole-resistant Candida albicans
AbstractCandida species is the causative agent in approximately 80% of invasive mycoses and drug-resistantCandida albicans is among the four strains of ‘critical priority group’ framed by WHO. Lichens are endowed with some rare phytochemicals and a plethora of therapeutics viz. antifungal capacities ofRoccella montagnei. Biosynthesis of silver nanoparticles (AgNPs) using lichen could offer an eco-friendly, and cost-effective alternative against emerging ‘microbial resistance.’ Therefore, the objective was to biosynthesize silver nanoparticles (Rm-AgNPs) using a Hydro-alcoholic (1:1) extract ofR. montagnei to develo...
Source: World Journal of Microbiology and Biotechnology - April 9, 2024 Category: Microbiology Source Type: research

Analysis of the bacterial diversity in Moroccan Jben cheese using TTGE, DGGE, and 16S rRNA sequencing
AbstractThis research investigated the physicochemical, microbiological, and bacterial diversity of Jben cheese, a popular artisanal variety in Morocco. The bacterial diversity was explored using culture-independent methods, including temporal temperature gel electrophoresis (TTGE), denaturing gradient gel electrophoresis (DGGE), and high-throughput sequencing (HTS). Significant intra-sample differences were observed for most physicochemical parameters within each milk type, while inter-sample differences occurred between cow and goat cheeses for dry matter and ash. Jben cheese exhibited distinct characteristics, with low ...
Source: World Journal of Microbiology and Biotechnology - April 9, 2024 Category: Microbiology Source Type: research

Transcriptional regulators of secondary metabolite biosynthesis in Streptomyces
AbstractIn the post-genome era, great progress has been made in metabolic engineering using recombinant DNA technology to enhance the production of high-value products byStreptomyces. With the development of microbial genome sequencing techniques and bioinformatic tools, a growing number of secondary metabolite (SM) biosynthetic gene clusters inStreptomyces and their biosynthetic logics have been uncovered and elucidated. In order to increase our knowledge about transcriptional regulators in SM ofStreptomyces, this review firstly makes a comprehensive summary of the characterized factors involved in enhancing SM production...
Source: World Journal of Microbiology and Biotechnology - April 8, 2024 Category: Microbiology Source Type: research

Kinetic modeling and optimization of ethanol fermentation by the marine yeast Wickerhamomyces subpelliculosus ZE75
AbstractThe study aims to enhance ethanol production byWickerhamomyces subpelliculosus ZE75 isolated from marine sediment. In addition, analyzing the kinetic parameters of ethanol production and optimization of the fermentation conditions was performed. The marine yeast isolate ZE75 was selected as the front runner ethanol-producer, with an ethanol yield of 89.77  gL−1. ZE75 was identified relying on the phenotypic and genotypic characteristics ofW. subpelliculosus. The genotypic characterization based on the Internal Transcribed Spacer (ITS) sequence was deposited in the GenBank database with the accession number OP715...
Source: World Journal of Microbiology and Biotechnology - April 6, 2024 Category: Microbiology Source Type: research

Metabolic engineering Corynebacterium glutamicum for D-chiro-inositol production
AbstractD-chiro-inositol (DCI) is a potential drug for the treatment of type II diabetes and polycystic ovary syndrome. In order to effectively synthesize DCI inCorynebacterium glutamicum, the genes related to inositol catabolism in clustersiol1 andiol2 were knocked out inC. glutamicum SN01 to generate the chassis strain DCI-1. DCI-1 did not grow in and catabolizemyo-inositol (MI). Subsequently, different exogenous and endogenous inosose isomerases were expressed in DCI-1 and their conversion ability of DCI from MI were compared. After fermentation, the strain DCI-7 co-expressing inosose isomerase IolI2 and inositol dehydr...
Source: World Journal of Microbiology and Biotechnology - April 3, 2024 Category: Microbiology Source Type: research

Navigating the signaling landscape of Ralstonia solanacearum: a study of bacterial two-component systems
AbstractRalstonia solanacearum, the bacterium that causes bacterial wilt, is a destructive phytopathogen that can infect over 450 different plant species. Several agriculturally significant crop plants, including eggplant, tomato, pepper, potato, and ginger, are highly susceptible to this plant disease, which has a global impact on crop quality and yield. There is currently no known preventive method that works well for bacterial wilt. Bacteria use two-component systems (TCSs) to sense their environment constantly and react appropriately. This is achieved by an extracellular sensor kinase (SK) capable of sensing a suitable...
Source: World Journal of Microbiology and Biotechnology - April 2, 2024 Category: Microbiology Source Type: research

Microbial innovations in chromium remediation: mechanistic insights and diverse applications
AbstractThe ubiquity of hexavalent chromium (Cr(VI)) from industrial activities poses a critical environmental threat due to its persistence, toxicity and mutagenic potential. Traditional physico-chemical methods for its removal often entail significant environmental drawbacks. Recent advancements in remediation strategies have emphasized nano and bioremediation techniques as promising avenues for cost-effective and efficient Cr(VI) mitigation. Bioremediation harnesses the capabilities of biological agents like microorganisms, and algae to mitigate heavy metal contamination, while nano-remediation employs nanoparticles for...
Source: World Journal of Microbiology and Biotechnology - March 30, 2024 Category: Microbiology Source Type: research

Saponaria officinalis saponins as a factor increasing permeability of Candida yeasts ’ biomembrane
AbstractSaponins are a large group of compounds, produced mostly by plants as a side product of their metabolic activity. These compounds have attracted much attention over the years mostly because of their surface activity and antibacterial, anti-inflammatory and antifungal properties. On the other hand, most of the hitherto research has concerned the action of saponins against microbial cells as a whole. Therefore, knowing the possible interaction of saponins with biomembrane, we decided to check in-vitro the influence of saponin-rich extract ofSaponaria officinalis on spheroplasts of twoCandida sp. The obtained results ...
Source: World Journal of Microbiology and Biotechnology - March 30, 2024 Category: Microbiology Source Type: research

Immobilized microalgae: principles, processes and its applications in wastewater treatment
AbstractMicroalgae have emerged as potential candidates for biomass production and pollutant removal. However, expensive biomass harvesting, insufficient biomass productivity, and low energy intensity limit the large-scale production of microalgae. To break through these bottlenecks, a novel technology of immobilized microalgae culture coupled with wastewater treatment has received increasing attention in recent years. In this review, the characteristics of two immobilized microalgae culture technologies are first presented and then their mechanisms are discussed in terms of biofilm formation theories, including thermodyna...
Source: World Journal of Microbiology and Biotechnology - March 29, 2024 Category: Microbiology Source Type: research

Agro-ecosystem of honeybees as source for native probiotic yeasts
AbstractProbiotic microorganisms are used to improve the health and wellness of people and the research on this topic is of current relevance and interest. Fifty-five yeasts, coming from honeybee ’s ecosystem and belonging toCandida,Debaryomyces,Hanseniaspora,Lachancea,Metschnikowia,Meyerozyma,Starmerella andZygosacchromyces genera and related different species, were evaluated for the probiotic traits. The resistance to gastrointestinal conditions, auto-aggregation, cell surface hydrophobicity or biofilm formation abilities as well as antimicrobial activity against common human pathogenic bacteria were evaluated. The saf...
Source: World Journal of Microbiology and Biotechnology - March 28, 2024 Category: Microbiology Source Type: research