Comparative in vitro toxicological effects of water-soluble and insoluble components of atmospheric PM < sub > 2.5 < /sub > on human lung cells
Toxicol In Vitro. 2024 Apr 13:105828. doi: 10.1016/j.tiv.2024.105828. Online ahead of print.ABSTRACTFine particulates in city air significantly impact human health, but the hazardous compositional mechanisms are still unclear. Besides the toxicity of environmental PM2.5 to in vitro human lung epithelial cells (A549), the independent cytotoxicity of PM2.5-bound water-soluble (WS-PM2.5) and water-insoluble (WIS-PM2.5) fractions were also compared by cell viability, oxidative stress (reactive oxygen species, ROS), and inflammatory injury (IL-6 and TNF-α). The cytotoxicity of PM2.5 varied significantly by sampling season and ...
Source: Toxicology in Vitro - April 15, 2024 Category: Toxicology Authors: Yaqian Wei Yan Chen Youwei Hong Jinsheng Chen Hong-Bo Li Hanhan Li Xuewen Yao Tariq Mehmood Xinyuan Feng Xiao-San Luo Source Type: research
Comparative in vitro toxicological effects of water-soluble and insoluble components of atmospheric PM < sub > 2.5 < /sub > on human lung cells
Toxicol In Vitro. 2024 Apr 13:105828. doi: 10.1016/j.tiv.2024.105828. Online ahead of print.ABSTRACTFine particulates in city air significantly impact human health, but the hazardous compositional mechanisms are still unclear. Besides the toxicity of environmental PM2.5 to in vitro human lung epithelial cells (A549), the independent cytotoxicity of PM2.5-bound water-soluble (WS-PM2.5) and water-insoluble (WIS-PM2.5) fractions were also compared by cell viability, oxidative stress (reactive oxygen species, ROS), and inflammatory injury (IL-6 and TNF-α). The cytotoxicity of PM2.5 varied significantly by sampling season and ...
Source: Toxicology in Vitro - April 15, 2024 Category: Toxicology Authors: Yaqian Wei Yan Chen Youwei Hong Jinsheng Chen Hong-Bo Li Hanhan Li Xuewen Yao Tariq Mehmood Xinyuan Feng Xiao-San Luo Source Type: research
Effects of iron oxide nanoparticles on the gene expression profiles of cerebral endotheliocytes and astrocytes
Toxicol In Vitro. 2024 Apr 12:105829. doi: 10.1016/j.tiv.2024.105829. Online ahead of print.ABSTRACTIron oxide nanoparticles (IONPs) are considered as the most biocompatible magnetic materials suitable for biomedical applications. Nevertheless, there are many evidences of their toxicity for living organisms and partially neurotoxicity. The central nervous system is protected from undesirable substances circulating in the bloodstream by the blood-brain barrier (BBB). And even if being small enough, some nanoparticles could be able to penetrate cell membranes in other cells but will often be delayed by the BBB cells. However...
Source: Toxicology in Vitro - April 14, 2024 Category: Toxicology Authors: Anna Tyumentseva Elena Khilazheva Valeria Petrova Sergey Stolyar Source Type: research
Evaluation of the impact of iPSC differentiation protocols on transcriptomic signatures
In conclusion, these data confirm the versatile use of iPSC differentiated cell types as standardizable and relevant model systems for in vitro toxicology.PMID:38615723 | DOI:10.1016/j.tiv.2024.105826 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - April 14, 2024 Category: Toxicology Authors: Vidya Chandrasekaran Sara Wellens Aurore Bourguignon Ivo Djidrovski Leonie Fransen Sreya Ghosh Zahra Mazidi Cormac Murphy Carolina Nunes Pranika Singh Melinda Zana Lyle Armstrong Andr ás Dinnyés Johannes Grillari Regina Grillari-Voglauer Martin O Leonar Source Type: research
Risk assessment of Benzene, Toluene, Ethyl benzene, and Xylene (BTEX) in the atmospheric air around the world: A review
Toxicol In Vitro. 2024 Apr 12:105825. doi: 10.1016/j.tiv.2024.105825. Online ahead of print.ABSTRACTVolatile organic compounds, such as BTEX, have been the subject of numerous debates due to their detrimental effects on the environment and human health. Human beings have had a significant role in the emergence of this situation. Even though US EPA, WHO, and other health-related organizations have set standard limits as unhazardous levels, it has been observed that within or even below these limits, constant exposure to these toxic chemicals results in negative consequences as well. According to these facts, various studies...
Source: Toxicology in Vitro - April 14, 2024 Category: Toxicology Authors: Mohammad Ali Zahed Samira Salehi Mahtab Akbarzadeh Khoei Pedram Esmaeili Leila Mohajeri Source Type: research
Effects of iron oxide nanoparticles on the gene expression profiles of cerebral endotheliocytes and astrocytes
Toxicol In Vitro. 2024 Apr 12:105829. doi: 10.1016/j.tiv.2024.105829. Online ahead of print.ABSTRACTIron oxide nanoparticles (IONPs) are considered as the most biocompatible magnetic materials suitable for biomedical applications. Nevertheless, there are many evidences of their toxicity for living organisms and partially neurotoxicity. The central nervous system is protected from undesirable substances circulating in the bloodstream by the blood-brain barrier (BBB). And even if being small enough, some nanoparticles could be able to penetrate cell membranes in other cells but will often be delayed by the BBB cells. However...
Source: Toxicology in Vitro - April 14, 2024 Category: Toxicology Authors: Anna Tyumentseva Elena Khilazheva Valeria Petrova Sergey Stolyar Source Type: research
Evaluation of the impact of iPSC differentiation protocols on transcriptomic signatures
In conclusion, these data confirm the versatile use of iPSC differentiated cell types as standardizable and relevant model systems for in vitro toxicology.PMID:38615723 | DOI:10.1016/j.tiv.2024.105826 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - April 14, 2024 Category: Toxicology Authors: Vidya Chandrasekaran Sara Wellens Aurore Bourguignon Ivo Djidrovski Leonie Fransen Sreya Ghosh Zahra Mazidi Cormac Murphy Carolina Nunes Pranika Singh Melinda Zana Lyle Armstrong Andr ás Dinnyés Johannes Grillari Regina Grillari-Voglauer Martin O Leonar Source Type: research
Risk assessment of Benzene, Toluene, Ethyl benzene, and Xylene (BTEX) in the atmospheric air around the world: A review
Toxicol In Vitro. 2024 Apr 12:105825. doi: 10.1016/j.tiv.2024.105825. Online ahead of print.ABSTRACTVolatile organic compounds, such as BTEX, have been the subject of numerous debates due to their detrimental effects on the environment and human health. Human beings have had a significant role in the emergence of this situation. Even though US EPA, WHO, and other health-related organizations have set standard limits as unhazardous levels, it has been observed that within or even below these limits, constant exposure to these toxic chemicals results in negative consequences as well. According to these facts, various studies...
Source: Toxicology in Vitro - April 14, 2024 Category: Toxicology Authors: Mohammad Ali Zahed Samira Salehi Mahtab Akbarzadeh Khoei Pedram Esmaeili Leila Mohajeri Source Type: research
Sens-ocular model: Cell-based assay to evaluate eye stinging potential of chemicals and baby cosmetic formulations
In this study, HEK-293 cells with high TRPV1 expression were utilized to evaluate calcium influx related to receptor activation triggered by chemicals and cosmetic formulations. The cells were exposed to increasing concentrations of substances to cause or not some aggression to the eye, and TRPV1 activity was assessed by measuring intracellular FURA-2 AM fluorescence signal. To confirm TRPV1 channel activation, capsazepine, a capsaicin antagonist, was employed in addition to using capsaicin as a positive control. The study's results indicate that this novel model can identify compounds known to cause some aggression to the...
Source: Toxicology in Vitro - April 13, 2024 Category: Toxicology Authors: Lara Barroso Brito Carolina Motter Catarino Maria Claudia Passos Artur C Garcia Silva Desiree Cigaran Schuck Andrezza Di Pietro Micali Canavez Marize Campos Valadares Source Type: research
Sens-ocular model: Cell-based assay to evaluate eye stinging potential of chemicals and baby cosmetic formulations
In this study, HEK-293 cells with high TRPV1 expression were utilized to evaluate calcium influx related to receptor activation triggered by chemicals and cosmetic formulations. The cells were exposed to increasing concentrations of substances to cause or not some aggression to the eye, and TRPV1 activity was assessed by measuring intracellular FURA-2 AM fluorescence signal. To confirm TRPV1 channel activation, capsazepine, a capsaicin antagonist, was employed in addition to using capsaicin as a positive control. The study's results indicate that this novel model can identify compounds known to cause some aggression to the...
Source: Toxicology in Vitro - April 13, 2024 Category: Toxicology Authors: Lara Barroso Brito Carolina Motter Catarino Maria Claudia Passos Artur C Garcia Silva Desiree Cigaran Schuck Andrezza Di Pietro Micali Canavez Marize Campos Valadares Source Type: research
Evaluating the QileX-RhE skin corrosion test for chemical subcategorization in accordance with OECD TG 431
This study evaluates the QileX-RhE test system's performance for chemical subcategorization within the OECD TG 431 framework. Results demonstrate its ability to differentiate subcategories, accurately predicting 83% of UN GHS Category 1A and 73% of UN GHS Category 1B/1C chemicals with 100% sensitivity in corrosive prediction. Additionally, this study provides a comprehensive assessment of the test method's performance by employing nuanced parameters such as positive predictive value (PPV), negative predictive value (NPV), post-test odds and likelihood rations, offering valuable insights into the applicability and effective...
Source: Toxicology in Vitro - April 11, 2024 Category: Toxicology Authors: M Chac ón N V ázquez S Alonso-Alonso S Llames M Pevida I Alcalde J Merayo-Lloves Á Meana Source Type: research
Evaluating the QileX-RhE skin corrosion test for chemical subcategorization in accordance with OECD TG 431
This study evaluates the QileX-RhE test system's performance for chemical subcategorization within the OECD TG 431 framework. Results demonstrate its ability to differentiate subcategories, accurately predicting 83% of UN GHS Category 1A and 73% of UN GHS Category 1B/1C chemicals with 100% sensitivity in corrosive prediction. Additionally, this study provides a comprehensive assessment of the test method's performance by employing nuanced parameters such as positive predictive value (PPV), negative predictive value (NPV), post-test odds and likelihood rations, offering valuable insights into the applicability and effective...
Source: Toxicology in Vitro - April 11, 2024 Category: Toxicology Authors: M Chac ón N V ázquez S Alonso-Alonso S Llames M Pevida I Alcalde J Merayo-Lloves Á Meana Source Type: research
Hemocompatibility studies in nanotoxicology: Hemolysis or eryptosis? (A review)
Toxicol In Vitro. 2024 Apr 4;98:105814. doi: 10.1016/j.tiv.2024.105814. Online ahead of print.ABSTRACTHemocompatibility evaluation is an important step in nanotoxicological studies. It is generally accepted that nanomaterials promote lysis of erythrocytes, blood clotting, alter phagocytosis, and upregulate pro-inflammatory cytokines. However, there are no standardized guidelines for testing nanomaterials hemocompatibility despite the fact that nanomaterials enter the bloodstream and interact with blood cells. In this review, the current knowledge on the ability of nanomaterials to induce distinct cell death modalities of e...
Source: Toxicology in Vitro - April 6, 2024 Category: Toxicology Authors: Anton Tkachenko Source Type: research
Hemocompatibility studies in nanotoxicology: Hemolysis or eryptosis? (A review)
Toxicol In Vitro. 2024 Apr 4;98:105814. doi: 10.1016/j.tiv.2024.105814. Online ahead of print.ABSTRACTHemocompatibility evaluation is an important step in nanotoxicological studies. It is generally accepted that nanomaterials promote lysis of erythrocytes, blood clotting, alter phagocytosis, and upregulate pro-inflammatory cytokines. However, there are no standardized guidelines for testing nanomaterials hemocompatibility despite the fact that nanomaterials enter the bloodstream and interact with blood cells. In this review, the current knowledge on the ability of nanomaterials to induce distinct cell death modalities of e...
Source: Toxicology in Vitro - April 6, 2024 Category: Toxicology Authors: Anton Tkachenko Source Type: research
Hemocompatibility studies in nanotoxicology: Hemolysis or eryptosis? (A review)
Toxicol In Vitro. 2024 Apr 4;98:105814. doi: 10.1016/j.tiv.2024.105814. Online ahead of print.ABSTRACTHemocompatibility evaluation is an important step in nanotoxicological studies. It is generally accepted that nanomaterials promote lysis of erythrocytes, blood clotting, alter phagocytosis, and upregulate pro-inflammatory cytokines. However, there are no standardized guidelines for testing nanomaterials hemocompatibility despite the fact that nanomaterials enter the bloodstream and interact with blood cells. In this review, the current knowledge on the ability of nanomaterials to induce distinct cell death modalities of e...
Source: Toxicology in Vitro - April 6, 2024 Category: Toxicology Authors: Anton Tkachenko Source Type: research
