Stromal rigidity stress accelerates pancreatic intraepithelial neoplasia progression and chromosomal instability via nuclear PTK2 localization
Am J Pathol. 2024 Apr 15:S0002-9440(24)00132-9. doi: 10.1016/j.ajpath.2024.02.023. Online ahead of print.ABSTRACTSince the mechanotransduction by stromal stiffness stimulates the rupture and repair of the nuclear envelope in pancreatic progenitor cells, accumulated genomic aberrations are under selection in the tumor microenvironment. Analysis of cell growth, micronuclei, and γH2AX foci links to mechanotransduction pressure in vivo during serial orthotopic passages of mouse KrasLSL-G12D/+;Trp53flox/flox;Pdx1-Cre (KPC) cancer cells in the tumor and in migrating through the size-restricted 3μm micropores. To search for pan...
Source: The American Journal of Pathology - April 17, 2024 Category: Pathology Authors: Li-Yun Ding Chia-Jung Chang Szu-Ying Chen Kuan-Lin Chen Yueh-Shan Li Yun-Chieh Wu Ting-Yi Hsu Hsin-Yu Ying Hsin-Yi Wu Michael W Hughes Chia-Yih Wang Chih-Han Chang Ming-Jer Tang Woei-Jer Chuang Yan-Shen Shan Chia-Jung Chang Po-Hsien Huang Source Type: research
This Month in AJP
Am J Pathol. 2024 Apr 8:S0002-9440(24)00126-3. doi: 10.1016/j.ajpath.2024.04.001. Online ahead of print.NO ABSTRACTPMID:38599460 | DOI:10.1016/j.ajpath.2024.04.001 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Source Type: research
Differentiation of Type 17 Mucosal-Associated Invariant T Cells in Circulation Contributes to the Severity of Sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RAR-related orphan receptor γt in patients with sepsis. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA-sequencing analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in patients with sepsis. Cell-culture experiments demonstrated that phosphoinositide 3-kinase-LDHA signaling was required for RAR-related orphan receptor γt expression in MAIT17. Finally, the elevated levels of plasma IL-18 promoted the differentiation of circulating MAIT17 cells in sepsis. In su...
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Authors: Xinying Li Sicheng Fu Hao Cheng Min Ma Zijian Song Jun Li Shuang Wu Chong Zhang Xiaoxia Wang Maoyu Tang Xuexue Pu Qiang Ji Jinquan Liang Zhibin Zhao Heinrich K örner Bin Li Min Shao Hua Wang Source Type: research
This Month in AJP
Am J Pathol. 2024 Apr 8:S0002-9440(24)00126-3. doi: 10.1016/j.ajpath.2024.04.001. Online ahead of print.NO ABSTRACTPMID:38599460 | DOI:10.1016/j.ajpath.2024.04.001 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Source Type: research
Differentiation of type 17 MAIT cells in circulation contributes to the severity of sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RORγt in sepsis patients. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA sequencing (scRNA-seq) analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in sepsis patients. Cell-culture experiments demonstrated that Phosphoinositide 3-kinase (PI3K)-LDHA signaling was required for RORγt expression in MAIT17. Finally, the elevated levels of plasma IL-18 promoted the differentiation of circulating MAIT17 in sepsis. In summary, this study reveals a new role of circula...
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Authors: Xinying Li Sicheng Fu Hao Cheng Min Ma Zijian Song Jun Li Shuang Wu Chong Zhang Xiaoxia Wang Maoyu Tang Xuexue Pu Qiang Ji Jinquan Liang Zhibin Zhao Heinrich K örner Bin Li Min Shao Hua Wang Source Type: research
This Month in AJP
Am J Pathol. 2024 Apr 8:S0002-9440(24)00126-3. doi: 10.1016/j.ajpath.2024.04.001. Online ahead of print.NO ABSTRACTPMID:38599460 | DOI:10.1016/j.ajpath.2024.04.001 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Source Type: research
Differentiation of type 17 MAIT cells in circulation contributes to the severity of sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RORγt in sepsis patients. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA sequencing (scRNA-seq) analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in sepsis patients. Cell-culture experiments demonstrated that Phosphoinositide 3-kinase (PI3K)-LDHA signaling was required for RORγt expression in MAIT17. Finally, the elevated levels of plasma IL-18 promoted the differentiation of circulating MAIT17 in sepsis. In summary, this study reveals a new role of circula...
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Authors: Xinying Li Sicheng Fu Hao Cheng Min Ma Zijian Song Jun Li Shuang Wu Chong Zhang Xiaoxia Wang Maoyu Tang Xuexue Pu Qiang Ji Jinquan Liang Zhibin Zhao Heinrich K örner Bin Li Min Shao Hua Wang Source Type: research
This Month in AJP
Am J Pathol. 2024 Apr 8:S0002-9440(24)00126-3. doi: 10.1016/j.ajpath.2024.04.001. Online ahead of print.NO ABSTRACTPMID:38599460 | DOI:10.1016/j.ajpath.2024.04.001 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Source Type: research
Differentiation of type 17 MAIT cells in circulation contributes to the severity of sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RORγt in sepsis patients. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA sequencing (scRNA-seq) analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in sepsis patients. Cell-culture experiments demonstrated that Phosphoinositide 3-kinase (PI3K)-LDHA signaling was required for RORγt expression in MAIT17. Finally, the elevated levels of plasma IL-18 promoted the differentiation of circulating MAIT17 in sepsis. In summary, this study reveals a new role of circula...
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Authors: Xinying Li Sicheng Fu Hao Cheng Min Ma Zijian Song Jun Li Shuang Wu Chong Zhang Xiaoxia Wang Maoyu Tang Xuexue Pu Qiang Ji Jinquan Liang Zhibin Zhao Heinrich K örner Bin Li Min Shao Hua Wang Source Type: research
This Month in AJP
Am J Pathol. 2024 Apr 8:S0002-9440(24)00126-3. doi: 10.1016/j.ajpath.2024.04.001. Online ahead of print.NO ABSTRACTPMID:38599460 | DOI:10.1016/j.ajpath.2024.04.001 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Source Type: research
Differentiation of type 17 MAIT cells in circulation contributes to the severity of sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RORγt in sepsis patients. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA sequencing (scRNA-seq) analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in sepsis patients. Cell-culture experiments demonstrated that Phosphoinositide 3-kinase (PI3K)-LDHA signaling was required for RORγt expression in MAIT17. Finally, the elevated levels of plasma IL-18 promoted the differentiation of circulating MAIT17 in sepsis. In summary, this study reveals a new role of circula...
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Authors: Xinying Li Sicheng Fu Hao Cheng Min Ma Zijian Song Jun Li Shuang Wu Chong Zhang Xiaoxia Wang Maoyu Tang Xuexue Pu Qiang Ji Jinquan Liang Zhibin Zhao Heinrich K örner Bin Li Min Shao Hua Wang Source Type: research
This Month in AJP
Am J Pathol. 2024 Apr 8:S0002-9440(24)00126-3. doi: 10.1016/j.ajpath.2024.04.001. Online ahead of print.NO ABSTRACTPMID:38599460 | DOI:10.1016/j.ajpath.2024.04.001 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Source Type: research
Differentiation of type 17 MAIT cells in circulation contributes to the severity of sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RORγt in sepsis patients. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA sequencing (scRNA-seq) analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in sepsis patients. Cell-culture experiments demonstrated that Phosphoinositide 3-kinase (PI3K)-LDHA signaling was required for RORγt expression in MAIT17. Finally, the elevated levels of plasma IL-18 promoted the differentiation of circulating MAIT17 in sepsis. In summary, this study reveals a new role of circula...
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Authors: Xinying Li Sicheng Fu Hao Cheng Min Ma Zijian Song Jun Li Shuang Wu Chong Zhang Xiaoxia Wang Maoyu Tang Xuexue Pu Qiang Ji Jinquan Liang Zhibin Zhao Heinrich K örner Bin Li Min Shao Hua Wang Source Type: research
TFE3/PI3K/Akt/mTOR axis in renal cell carcinoma affects tumor microenvironment
Am J Pathol. 2024 Apr 6:S0002-9440(24)00122-6. doi: 10.1016/j.ajpath.2024.02.022. Online ahead of print.ABSTRACTThe role of the PI3K/Akt pathway in renal cell carcinoma (RCC) progression, metastasis, and resistance to therapies has not been thoroughly investigated. Transcription factor E3 (TFE3) expression is related to poorer prognosis and tumor microenvironment in patients with RCC. We aimed to determine the relationship between TFE3 and the PI3K/Akt pathway. TFE3 downregulation was achieved by transient transfection of small interfering RNA (siRNA) and short hairpin RNA (shRNA) in UOK146 cells. TFE3 overexpression was i...
Source: The American Journal of Pathology - April 8, 2024 Category: Pathology Authors: Chung Su Hwang Yun Kyung Kang Ji Yun Kim So Hyun Shin Joon Young Park Ji Sun Song So Young Kim Se Jin Jung Jung Hee Lee Ju-Young Na Dong Hoon Shin Jee Yeon Kim Sung Woo Park Hyun Jung Lee Source Type: research
Role of HNF4alpha-cMyc Interaction in CDE-diet Induced Liver Injury and Regeneration
This study investigated the role of HNF4α-cMyc interaction in regulating liver injury and regeneration using the choline-deficient and ethionine-supplemented (CDE) diet model. WT, hepatocyte-specific HNF4α-KO, cMyc-KO, and HNF4α-cMyc double knockout (DKO) mice were fed a CDE diet for one week to induce subacute liver injury. To study regeneration, CDE diet was followed by normal chow diet for one week. WT mice showed significant liver injury and decreased HNF4α mRNA and protein expression after one week of a CDE diet. HNF4α deletion resulted in significantly higher injury with increased inflammation, fibrosis, prolife...
Source: The American Journal of Pathology - April 8, 2024 Category: Pathology Authors: Manasi Kotulkar Julia Barbee Diego Paine-Cabrera Dakota Robarts Maura O'Neil Udayan Apte Source Type: research
