Author Index
(Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Tags: Volume 5 Author Index Source Type: research
Acknowledgments
(Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Tags: Volume 5 Acknowledgments Source Type: research
Translating Periosteum's Regenerative Power: Insights From Quantitative Analysis of Tissue Genesis With a Periosteum Substitute Implant
This study aimed to develop quantitative, efficacy-based measures, thereby providing translational guidelines for the use of periosteum to harness the body's own healing potential and generate target tissues. The current study quantitatively and qualitatively demonstrated tissue generation modulated by a periosteum substitute membrane that replicates the structural constituents of native periosteum (elastin, collagen, progenitor cells) and its barrier, extracellular, and cellular properties. It shows the potentiation of the periosteum's regenerative capacity through the progenitor cells that inhabit the tissue, biological ...
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Moore, S. R., Heu, C., Yu, N. Y. C., Whan, R. M., Knothe, U. R., Milz, S., Knothe Tate, M. L. Tags: Tissue Engineering and Regenerative Medicine, Mesenchymal Stem Cells Source Type: research
Spontaneous In Vivo Chondrogenesis of Bone Marrow-Derived Mesenchymal Progenitor Cells by Blocking Vascular Endothelial Growth Factor Signaling
This article outlines the general paradigm of controlling the fate of implanted stem/progenitor cells by engineering their ability to establish specific microenvironmental conditions rather than directly providing individual morphogenic cues.
Significance
Chondrogenic differentiation of mesenchymal stromal/stem cells (MSCs) is typically targeted by morphogen delivery, which is often associated with limited efficiency, stability, and robustness. This article proposes a strategy to engineer MSCs with the capacity to establish specific microenvironmental conditions, supporting their own targeted differentiation program. Sole...
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Marsano, A., Medeiros da Cunha, C. M., Ghanaati, S., Gueven, S., Centola, M., Tsaryk, R., Barbeck, M., Stuedle, C., Barbero, A., Helmrich, U., Schaeren, S., Kirkpatrick, J. C., Banfi, A., Martin, I. Tags: Tissue Engineering and Regenerative Medicine, Adult Stem Cells, Mesenchymal Stem Cells Source Type: research
Smooth Muscle Progenitor Cells Derived From Human Pluripotent Stem Cells Induce Histologic Changes in Injured Urethral Sphincter
We examined in vivo integration of smooth muscle progenitor cells (pSMCs) derived from human pluripotent stem cells (hPSCs). pSMCs were derived from a human embryonic stem cell line (H9-ESCs) and two induced pluripotent stem cell (iPSC) lines. pSMCs were injected periurethrally into urethral injury rat models (2 x 106 cells per rat) or intramuscularly into severe combined immunodeficiency mice. Histologic and quantitative image analysis revealed that the urethras in pSMC-treated rats contained abundant elastic fibers and thicker muscle layers compared with the control rats. Western blot confirmed increased elastin/collagen...
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Li, Y., Wen, Y., Wang, Z., Wei, Y., Wani, P., Green, M., Swaminathan, G., Ramamurthi, A., Pera, R. R., Chen, B. Tags: Tissue Engineering and Regenerative Medicine, Embryonic Stem Cells, Pluripotent Stem Cells Source Type: research
Human Endomyocardial Biopsy Specimen-Derived Stromal Cells Modulate Angiotensin II-Induced Cardiac Remodeling
This study demonstrates that cells derived from human endomyocardial biopsies, cardiac-derived adherent proliferating cells (CardAPs), have the potential to reduce angiotensin II-induced cardiac remodeling and improve left ventricular function in angiotensin II mice. The mechanism involves antifibrotic and antihypertrophic effects via paracrine actions and immunomodulatory properties. These findings support the potential of CardAPs for the treatment of heart failure. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Miteva, K., Van Linthout, S., Pappritz, K., Muller, I., Spillmann, F., Haag, M., Stachelscheid, H., Ringe, J., Sittinger, M., Tschope, C. Tags: Tissue Engineering and Regenerative Medicine, Mesenchymal Stem Cells Source Type: research
Hair Follicle and Sebaceous Gland De Novo Regeneration With Cultured Epidermal Stem Cells and Skin-Derived Precursors
In this study, transplantation of a combination of culture-expanded epidermal stem cells and skin-derived progenitors from mice and adult humans led to de novo regeneration of functional hair follicles and sebaceous glands. The data provide transferable knowledge for the development of novel bioengineered skin substitutes with epidermal appendage regeneration capacity. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Wang, X., Wang, X., Liu, J., Cai, T., Guo, L., Wang, S., Wang, J., Cao, Y., Ge, J., Jiang, Y., Tredget, E. E., Cao, M., Wu, Y. Tags: Tissue Engineering and Regenerative Medicine, Skin-Derived Precursors/Progenitors Source Type: research
Fat-Derived Stromal Vascular Fraction Cells Enhance the Bone-Forming Capacity of Devitalized Engineered Hypertrophic Cartilage Matrix
This study validates an innovative bone substitute material based on allogeneic hypertrophic cartilage that is engineered, devitalized, stored, and clinically used, together with autologous cells, intraoperatively derived from a lipoaspirate. The strategy was tested using human cells in an ectopic model and an orthotopic implantation model, in immunocompromised animals. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Todorov, A., Kreutz, M., Haumer, A., Scotti, C., Barbero, A., Bourgine, P. E., Scherberich, A., Jaquiery, C., Martin, I. Tags: Tissue Engineering and Regenerative Medicine, Mesenchymal Stem Cells, Adipose-Derived Stem Cells, IFATS Collection Source Type: research
Skeletal Muscle Differentiation on a Chip Shows Human Donor Mesoangioblasts Efficiency in Restoring Dystrophin in a Duchenne Muscular Dystrophy Model
This study aimed to provide in vitro quantitative evidence of the ability of mesoangioblasts to restore dystrophin, in terms of protein accumulation and distribution, within myotubes derived from DMD patients, using a microengineered model. We designed an ad hoc experimental strategy to miniaturize on a chip the standard process of muscle regeneration independent of variables such as inflammation and fibrosis. It is based on the coculture, at different ratios, of human dystrophin-positive myogenic progenitors and dystrophin-negative myoblasts in a substrate with muscle-like physiological stiffness and cell micropatterns. R...
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Serena, E., Zatti, S., Zoso, A., Lo Verso, F., Tedesco, F. S., Cossu, G., Elvassore, N. Tags: Mesangioblasts, Enabling Technologies for Cell-Based Clinical Translation Source Type: research
Biodistribution and Clearance of Human Mesenchymal Stem Cells by Quantitative Three-Dimensional Cryo-Imaging After Intravenous Infusion in a Rat Lung Injury Model
This article demonstrates, for the first time, the use of three-dimensional cryo-imaging for single-cell quantitative tracking of intravenous infused clinical-grade mesenchymal stem cells in a clinically relevant model of lung injury. The important information learned in this study will help guide future clinical and translational stem cell therapies for lung injuries. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Schmuck, E. G., Koch, J. M., Centanni, J. M., Hacker, T. A., Braun, R. K., Eldridge, M., Hei, D. J., Hematti, P., Raval, A. N. Tags: Mesenchymal Stem Cells, Enabling Technologies for Cell-Based Clinical Translation Source Type: research
miR-145 Regulates Diabetes-Bone Marrow Stromal Cell-Induced Neurorestorative Effects in Diabetes Stroke Rats
In rats with type 1 diabetes (T1DM), the therapeutic effects and underlying mechanisms of action of stroke treatment were compared between bone-marrow stromal cells (BMSCs) derived from T1DM rats (DM-BMSCs) and BMSCs derived from normal rats (Nor-BMSCs). The novel role of microRNA-145 (miR-145) in mediating DM-BMSC treatment-induced benefits was also investigated. T1DM rats (n = 8 per group) underwent 2 hours of middle cerebral artery occlusion (MCAo) and were treated 24 hours later with the one of the following (5 x 106 cells administered i.v.): (a) phosphate-buffered saline (PBS); (b) Nor-BMSCs; (c) DM-BMSCs; (d) DM-BMSC...
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Cui, C., Ye, X., Chopp, M., Venkat, P., Zacharek, A., Yan, T., Ning, R., Yu, P., Cui, G., Chen, J. Tags: Mesenchymal Stem Cells, Cell-Based Drug Development, Screening, and Toxicology Source Type: research
Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A
Niemann-Pick disease type A (NPA) is a lysosomal storage disease caused by mutations in the SMPD1 gene that encodes acid sphingomyelinase (ASM). Deficiency in ASM function results in lysosomal accumulation of sphingomyelin and neurodegeneration. Currently, there is no effective treatment for NPA. To accelerate drug discovery for treatment of NPA, we generated induced pluripotent stem cells from two patient dermal fibroblast lines and differentiated them into neural stem cells. The NPA neural stem cells exhibit a disease phenotype of lysosomal sphingomyelin accumulation and enlarged lysosomes. By using this disease model, w...
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Long, Y., Xu, M., Li, R., Dai, S., Beers, J., Chen, G., Soheilian, F., Baxa, U., Wang, M., Marugan, J. J., Muro, S., Li, Z., Brady, R., Zheng, W. Tags: Pluripotent Stem Cells, Cell-Based Drug Development, Screening, and Toxicology Source Type: research
Platelet-Derived Growth Factor-BB Protects Mesenchymal Stem Cells (MSCs) Derived From Immune Thrombocytopenia Patients Against Apoptosis and Senescence and Maintains MSC-Mediated Immunosuppression
In conclusion, we have demonstrated that PDGF-BB protects MSCs derived from ITP patients against apoptosis, senescence, and immunomodulatory defects. This protective effect of PDGF-BB is likely mediated via the p53/p21 pathway, thus potentially providing a new therapeutic approach for ITP.
Significance
Immune thrombocytopenia (ITP) is characterized by platelet destruction and megakaryocyte dysfunction. Platelet-derived growth factor (PDGF) improves growth and survival in various cell types and promotes mesenchymal stem cell (MSC) proliferation. PDGF-BB protects MSCs derived from ITP patients against apoptosis, senescence,...
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Zhang, J.-m., Feng, F.-e., Wang, Q.-m., Zhu, X.-l., Fu, H.-x., Xu, L.-p., Liu, K.-y., Huang, X.-j., Zhang, X.-h. Tags: Mesenchymal Stem Cells, Tissue-Specific Progenitor and Stem Cells Source Type: research
Mesenchymal Stem Cell-Derived Exosomes Promote Fracture Healing in a Mouse Model
This study evaluated the role of exosomes isolated from mesenchymal stem cell (MSC)-conditioned medium (MSC-CM) in the fracture-healing process of CD9–/– mice, a strain that is known to produce reduced levels of exosomes. Retardation of fracture healing in CD9–/– mice was rescued by the injection of MSC exosomes, but this was not the case after the injection of exosome-free CM. This study finds that MSC exosomes are a novel factor of MSC paracrine signaling, with an important role in the tissue repair process. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Furuta, T., Miyaki, S., Ishitobi, H., Ogura, T., Kato, Y., Kamei, N., Miyado, K., Higashi, Y., Ochi, M. Tags: Mesenchymal Stem Cells, Tissue-Specific Progenitor and Stem Cells Source Type: research
Clinical-Grade Human Multipotent Adult Progenitor Cells Block CD8+ Cytotoxic T Lymphocytes
This study confirms that these clinical-grade MAPCs are an immune-modulating population that inhibits CTL activation and effector responses and are, consequently, a highly valuable cell population for adoptive immunosuppressive therapy in diseases where damage is induced by CTLs.
Significance
Because multipotent adult progenitor cells (MAPCs) are among the noteworthy adult mesenchymal stem cell populations for immune therapy and have the advantage over mesenchymal stem cells (MSCs) of large-scale manufacturing and banking potential and thus prompt availability, it is important to understand how MAPCs interact with immune ...
Source: Stem Cells Translational Medicine - November 16, 2016 Category: Stem Cells Authors: Plessers, J., Dekimpe, E., Van Woensel, M., Roobrouck, V. D., Bullens, D. M., Pinxteren, J., Verfaillie, C. M., Van Gool, S. W. Tags: Adult Stem Cells, Mesenchymal Stem Cells, Tissue-Specific Progenitor and Stem Cells Source Type: research
