Retrospective study reveals the circulation of norovirus genotype GII.P21-GII.2 in Romania
Authors: Dinu S, Szmal C, Damian M, Oprişan G Abstract Noroviruses are the leading cause of acute gastroenteritis, causing significant economic burden globally. Infection is self-limiting, occurring as sporadic cases or producing outbreaks associated with consumption of contaminated water or food. All age groups are affected and person to person transmission is frequent. Except a recent outbreak in Romania caused by the emergent genotype GII.P17-GII.17, few data regarding the circulation of noroviruses in our country are available. We retrospectively analyzed stool samples from acute gastroenteritis patie...
Source: Roumanian Archives of Microbiology and Immunology - April 7, 2018 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

The impact of the molecular diagnosis in the surveillance of the Clostridium difficile infection.
Authors: Bãicuş A, Cîrstoiu M, Lambru K, Plata F, Cîrstoiu CF Abstract Clostridium difficile is the agent of many cases of antibiotic associated diarrhea. The prevalence of the toxigenic Clostridium difficile strains was assessed by real-time PCR between May 2014- January 2015, at the Emergency University Hospital, Bucharest, Romania. The incidence of the Clostridium difficile infection was 0.47%. Among the 1119 stool specimens tested, 165 (13.8%) were positive for C. difficile toxins A and B by immunochromatography test. All 165 positive samples were investigated by real time PCR and 134 (81%) samples...
Source: Roumanian Archives of Microbiology and Immunology - April 7, 2018 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Expression signature of some immunity genes triggered in Apis mellifera carpatica model by Pseudomonas entomophila experimental infection.
Authors: Neagu A, Raþiu AC, Mihalache (Radu) MR, Lazãr V, Chifiriuc MC, Ecovoiu AA Abstract Herein we report the expression profiles of certain immunity genes from Apis mellifera carpatica worker individuals experimentally infected with Pseudomonas entomophila L48 strain. Changes of the relative expression of abaecin, Relish, dorsal, Toll-1, domeless, and Duox genes were monitored by qRT-PCR. Our results were compared with similar ones from Drosophila melanogaster model and suggest that these genes are involved in the anti-infective defense mechanisms. Our study opens investigation avenues for modern pro...
Source: Roumanian Archives of Microbiology and Immunology - April 7, 2018 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Resistance pattern of multi-drug resistant strains of Mycobacterium tuberculosis and characteristics of patients with multi-drug resistant tuberculosis.
Conclusion: MDR strains of Mycobacterium tuberculosis display an increased resistance to first line anti-TB drugs. Extension of resistance to second line anti-TB drugs narrows the therapeutic options. Knowledge of MDR-TB risk factors is imperative for the correct and rapid initiation of the treatment. PMID: 29616530 [PubMed - in process] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - April 7, 2018 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Effects of Pseudomonas aeruginosa infection on the expression profile of transient receptor potential-like (TRPL) gene from Drosophila melanogaster.
Authors: Mihalache (Radu) MR, Raþiu AC, Neagu A, Lazãr V, Chifiriuc MC, Ecovoiu AA Abstract In an endeavor to monitor the effects of experimental infections with Pseudomonas aeruginosa on gene expression profiles of the eukaryotic model host, we focused on transient receptor potential-like (trpl) pleiotropic gene of Drosophila melanogaster (the fruit fly). Herein we report qRT-PCR data derived from experimental infections of male fruit flies with different genetic backgrounds by pricking and ingesta procedures, as compared to previous results obtained by microarray technology consecutive to ingesta exper...
Source: Roumanian Archives of Microbiology and Immunology - April 7, 2018 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Characterization of MRSA strains by phenotypic and OCR-based methods.
Authors: Luncã C, Iancu LS, Vremerã T, Tuchiluş CG, Dorneanu O Abstract With the emergence and spread of new methicilin resistant Staphylococcus aureus (MRSA) strains, control of dissemination, both in hospitals and in the community, requires the molecular characterization of the circulating strains in order to establish their dynamics and identify the sources of infection. During this study we analyzed the MRSA isolates by means of PCR-based methods in order to improve epidemiological surveillance and early application of prevention measures. The presence of mecA, nuc, lukF-PV and lukS-PV genes, as wel...
Source: Roumanian Archives of Microbiology and Immunology - April 7, 2018 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Polyploidization on SK-N-MC human neuroblastoma cells infected with herpes simplex virus 1.
We examined the impact of herpes simplex virus-1 (HSV-1) on SK-N-MC human neuroblastoma cell line. The infected cells were followed from 6 hours up to 96 hours post infection (hpi). A large number of polyploid cells with giant nuclei was observed under the influence of HSV-1 at 24 hpi with the DNA content of 32c to 64c or more, in comparison with control SK-N-MC cells that were characterized by relatively moderate values of ploidy, i.e. 8с to 16с (where 1c is the haploid amount of nuclear DNA found in normal diploid populations in G0/G1). After 48-96 hpi, the population of polyploid cells with giant nuclei decreased to t...
Source: Roumanian Archives of Microbiology and Immunology - April 7, 2018 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Short communication: Could the genexpert system be a new tool for poliovirus detection in the sewage water?
Authors: Bãicuş A Abstract Environmental enterovirus surveillance plays a key role in the detection and identification of importation of circulating vaccine-derived polioviruses into polio-free areas. In 2015, 2 cases of paralytic poliomyelitis caused by circulating vaccine-derived poliovirus type 1 (cVDPV1), from south-western Ukraine, bordering Romania, were confirmed. Romania was considered a country at risk and the environmental enterovirus surveillance was enhanced. In this context we tried to find a diagnosis algorithm for the rapid detection of poliovirus (PV) in the sewage water, using a combinat...
Source: Roumanian Archives of Microbiology and Immunology - April 7, 2018 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Mechanisms of resistance to ciprofloxacin and genetic diversity of escherichia coli strains originating from urine cultures performed for romanian adults.
MECHANISMS OF RESISTANCE TO CIPROFLOXACIN AND GENETIC DIVERSITY OF ESCHERICHIA COLI STRAINS ORIGINATING FROM URINE CULTURES PERFORMED FOR ROMANIAN ADULTS. Roum Arch Microbiol Immunol. 2015 Jul-Dec;74(3-4):73-8 Authors: Cristea VC, Oprea M, Neacşu G, Gîlcă R, Popa MI, Usein CR Abstract Urinary tract infections (UTI) with Escherichia coli are among the most common infections presenting in general practice. Fluoroquinolones (FQs) are relied on for their empirical therapy but recent reports indicate a concerning increase in the percentage of FQ-resistant E. coli isolates in many co...
Source: Roumanian Archives of Microbiology and Immunology - June 24, 2016 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Inhibition of virulence factors of pseudomonas aeruginosa by diclofenac sodium.
This study suggests the potential for the use of diclofenac sodium as an anti-virulence agent in the treatment of Pseudomonas aeruginosa infections. PMID: 27328521 [PubMed - in process] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - June 24, 2016 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Relative expression of efflux pumps in multi drug resistant pseudomonas aeruginosa.
RELATIVE EXPRESSION OF EFFLUX PUMPS IN MULTI DRUG RESISTANT PSEUDOMONAS AERUGINOSA. Roum Arch Microbiol Immunol. 2015 Jul-Dec;74(3-4):86-90 Authors: Azimi L, Namvar AE, Jamali S, Lari AR, Bijari A, Lari AR Abstract Pseudomonas aeruginosa is known as an important opportunistic pathogen, resistant to a high number of antibiotics. Efflux pumps are one of the main intrinsic antibiotics resistance mechanisms in P. aeruginosa. MexAB-OprM, MexCD-OprJ, and MexXY-OprM are the main efflux pumps involved in beta-lactam resistant strains which may cause cross resistance to different antimicro...
Source: Roumanian Archives of Microbiology and Immunology - June 24, 2016 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Study to establish the acceptance range for peroxyl radicals scavenger capacity of natural sod.
STUDY TO ESTABLISH THE ACCEPTANCE RANGE FOR PEROXYL RADICALS SCAVENGER CAPACITY OF NATURAL SOD. Roum Arch Microbiol Immunol. 2015 Jul-Dec;74(3-4):91-8 Authors: Lupu AR, Cremer L Abstract In the context of an emerging market of food supplements, the proven quality of the antioxidant products should be the main criteria for using them. The production process has to be carefully controlled and complementary tests are needed to demonstrate the correspondence between real and declared properties of final product. Using well characterized compounds with proven antioxidant activity in bi...
Source: Roumanian Archives of Microbiology and Immunology - June 24, 2016 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Immunological implications in mammalian semi-allogeneic pregnancy.
IMMUNOLOGICAL IMPLICATIONS IN MAMMALIAN SEMI-ALLOGENEIC PREGNANCY. Roum Arch Microbiol Immunol. 2015 Jul-Dec;74(3-4):99-104 Authors: Krupanidhi S Abstract The mammalian conceptus is a semi-allograft. The maternal tolerance towards semi-allograft is primed by a battery of cytokines and resident decidual cells. Invasion of embryonic trophoblast, decidual vascular remodeling and unresponsive incipient 'Triple-negative' (CD3-, CD4- and CD8-) T-cells of foetus promote normal pregnancy. On the other hand, the maternal cytokine profile shift towards Th1 response is shown to enhance the r...
Source: Roumanian Archives of Microbiology and Immunology - June 24, 2016 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Serum prolactin in melanoma patients with interferon alpha2b treatment.
CONCLUSIONS: The treatment with interferon alpha2b stimulated reversible and non-cumulative prolactin production. Evaluating prolactin in melanoma patients could become necessary in the future, both for finding a possible pituitary disorder, but also for a new pharmacological intervention. PMID: 27328525 [PubMed - in process] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - June 24, 2016 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research

Serological response to vaccines in children with diabetes.
CONCLUSION: Given the results of the study, no difference was found between patients with diabetes and controls who were vaccinated with pertussis, diphtheria, tetanus, mumps and HB vaccines. But there are some concerns about measles and rubella vaccinations that need further investigation. PMID: 27328526 [PubMed - in process] (Source: Roumanian Archives of Microbiology and Immunology)
Source: Roumanian Archives of Microbiology and Immunology - June 24, 2016 Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research