Japanese Planocerid Flatworms: Difference in Composition of Tetrodotoxin and Its Analogs and the Effects of Ingestion by Toxin-Bearing Fishes in the Ryukyu Islands, Japan
Mar Biotechnol (NY). 2024 Apr 17. doi: 10.1007/s10126-024-10312-0. Online ahead of print.ABSTRACTTetrodotoxin (TTX), known as pufferfish toxin, is a potent neurotoxin blocking sodium channels in muscle and nerve tissues. TTX has been detected in various taxa other than pufferfish, including marine polyclad flatworms, suggesting that pufferfish toxin accumulates in fish bodies via food webs. The composition of TTX and its analogs in the flatworm Planocera multitentaculata was identical to those in wild grass puffer Takifugu alboplumbeus. Previously, Planocera sp. from Okinawa Island, Japan, were reported to possess high lev...
Source: Marine Biotechnology - April 17, 2024 Category: Biotechnology Authors: Hiroyuki Ueda Masaaki Ito Ryo Yonezawa Kentaro Hayashi Taiga Tomonou Maho Kashitani Hikaru Oyama Kyoko Shirai Rei Suo Kazutoshi Yoshitake Shigeharu Kinoshita Shuichi Asakawa Shiro Itoi Source Type: research
Japanese Planocerid Flatworms: Difference in Composition of Tetrodotoxin and Its Analogs and the Effects of Ingestion by Toxin-Bearing Fishes in the Ryukyu Islands, Japan
Mar Biotechnol (NY). 2024 Apr 17. doi: 10.1007/s10126-024-10312-0. Online ahead of print.ABSTRACTTetrodotoxin (TTX), known as pufferfish toxin, is a potent neurotoxin blocking sodium channels in muscle and nerve tissues. TTX has been detected in various taxa other than pufferfish, including marine polyclad flatworms, suggesting that pufferfish toxin accumulates in fish bodies via food webs. The composition of TTX and its analogs in the flatworm Planocera multitentaculata was identical to those in wild grass puffer Takifugu alboplumbeus. Previously, Planocera sp. from Okinawa Island, Japan, were reported to possess high lev...
Source: Marine Biotechnology - April 17, 2024 Category: Biotechnology Authors: Hiroyuki Ueda Masaaki Ito Ryo Yonezawa Kentaro Hayashi Taiga Tomonou Maho Kashitani Hikaru Oyama Kyoko Shirai Rei Suo Kazutoshi Yoshitake Shigeharu Kinoshita Shuichi Asakawa Shiro Itoi Source Type: research
cAMP-Mediated CREM-MITF-TYR Axis Regulates Melanin Synthesis in Pacific Oysters
In this study, we performed in vitro and in vivo experiments to determine the role of cAMP in regulating melanogenesis in Pacific oysters. Besides, the function of cAMP-responsive element modulator (CREM) and the interactions between CREM and melanogenic genes were investigated. Our results showed that a high level of cAMP promotes the expression of melanogenic genes in Pacific oysters. CREM controls the expression of the MITF gene under cAMP regulation. In addition, CREM can regulate melanogenic gene expression, tyrosine metabolism, and melanin synthesis. These results indicate that cAMP plays an important role in the reg...
Source: Marine Biotechnology - April 13, 2024 Category: Biotechnology Authors: Kunyin Jiang Hong Yu Lingfeng Kong Shikai Liu Qi Li Source Type: research
cAMP-Mediated CREM-MITF-TYR Axis Regulates Melanin Synthesis in Pacific Oysters
In this study, we performed in vitro and in vivo experiments to determine the role of cAMP in regulating melanogenesis in Pacific oysters. Besides, the function of cAMP-responsive element modulator (CREM) and the interactions between CREM and melanogenic genes were investigated. Our results showed that a high level of cAMP promotes the expression of melanogenic genes in Pacific oysters. CREM controls the expression of the MITF gene under cAMP regulation. In addition, CREM can regulate melanogenic gene expression, tyrosine metabolism, and melanin synthesis. These results indicate that cAMP plays an important role in the reg...
Source: Marine Biotechnology - April 13, 2024 Category: Biotechnology Authors: Kunyin Jiang Hong Yu Lingfeng Kong Shikai Liu Qi Li Source Type: research
The lgi-miR-2d is Potentially Involved in Shell Melanin Synthesis by Targeting mitf in Manila Clam Ruditapes philippinarum
In this study, we investigated the function of lgi-miR-2d in shell melanin formation and the expression patterns of lgi-miR-2d and target gene Rpmitf in Manila clam Ruditapes philippinarum. We further explored and verified the relationship between Rpmitf and lgi-miR-2d and identified the expression level of shell color-related gene changes by RNAi and injecting the antagomir of lgi-miR-2d, respectively. Our results indicated that lgi-miR-2d antagomir affected the expression of its target gene Rpmitf. In addition, the dual-luciferase reporter assay was conducted to confirm the direct interaction between lgi-miR-2d and Rpmit...
Source: Marine Biotechnology - April 12, 2024 Category: Biotechnology Authors: Qiaoyue Xu Hongtao Nie Qianying Ma Jiadi Wang Zhongming Huo Xiwu Yan Source Type: research
The lgi-miR-2d is Potentially Involved in Shell Melanin Synthesis by Targeting mitf in Manila Clam Ruditapes philippinarum
In this study, we investigated the function of lgi-miR-2d in shell melanin formation and the expression patterns of lgi-miR-2d and target gene Rpmitf in Manila clam Ruditapes philippinarum. We further explored and verified the relationship between Rpmitf and lgi-miR-2d and identified the expression level of shell color-related gene changes by RNAi and injecting the antagomir of lgi-miR-2d, respectively. Our results indicated that lgi-miR-2d antagomir affected the expression of its target gene Rpmitf. In addition, the dual-luciferase reporter assay was conducted to confirm the direct interaction between lgi-miR-2d and Rpmit...
Source: Marine Biotechnology - April 12, 2024 Category: Biotechnology Authors: Qiaoyue Xu Hongtao Nie Qianying Ma Jiadi Wang Zhongming Huo Xiwu Yan Source Type: research
Development of a Rapid Detection Method to Prorocentrum lima by Loop-Mediated Isothermal Amplification with Hydroxy Naphthol Blue
In this study, a rapid, sensitive, and cost-effective detection method based on loop-mediated isothermal amplification (LAMP) was developed to identify P. lima. In this method, cell extracts of P. lima were diluted and used directly as templates for amplification, eliminating the need for nucleic acid purification and simplifying the detection process. Hydroxy naphthol blue (HNB) was incorporated into the reaction mix to facilitate result interpretation, enabling visual determination of the amplification outcome with the naked eye. The entire detection process, from DNA extraction to template amplification and product dete...
Source: Marine Biotechnology - April 11, 2024 Category: Biotechnology Authors: Chao Yang Yu Zhen Jialin Hou Tiezhu Mi Source Type: research
Development of a Rapid Detection Method to Prorocentrum lima by Loop-Mediated Isothermal Amplification with Hydroxy Naphthol Blue
In this study, a rapid, sensitive, and cost-effective detection method based on loop-mediated isothermal amplification (LAMP) was developed to identify P. lima. In this method, cell extracts of P. lima were diluted and used directly as templates for amplification, eliminating the need for nucleic acid purification and simplifying the detection process. Hydroxy naphthol blue (HNB) was incorporated into the reaction mix to facilitate result interpretation, enabling visual determination of the amplification outcome with the naked eye. The entire detection process, from DNA extraction to template amplification and product dete...
Source: Marine Biotechnology - April 11, 2024 Category: Biotechnology Authors: Chao Yang Yu Zhen Jialin Hou Tiezhu Mi Source Type: research
Production of Antioxidant, Angiotensin-Converting Enzyme Inhibitory and Osteogenic Gelatin Hydrolysate from Labeo rohita Swim Bladder
Mar Biotechnol (NY). 2024 Apr 1. doi: 10.1007/s10126-024-10305-z. Online ahead of print.ABSTRACTOptimization of antioxidants and angiotensin-converting enzyme (ACE) inhibitory potential gelatin hydrolysate production from Labeo rohita (rohu) swim bladder (SBGH) by alcalase using central composite design (CCD) of response surface methodology (RSM) was investigated. The maximum degree of hydrolysis (DH), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), total antioxidants (TAO), and ACE inhibitory activity were achieved at 0.1:1.0 (w/w) enzyme to substrate ratio, 61 °C hydro...
Source: Marine Biotechnology - April 1, 2024 Category: Biotechnology Authors: Balaji Wamanrao Kanwate Kalpana Patel Sandesh Suresh Karkal Deependra Rajoriya Kunal Sharan Tanaji G Kudre Source Type: research
Correction: Highly Efficient Genetic Transformation Methods for the Marine Oleaginous Diatom Fistulifera solaris
Mar Biotechnol (NY). 2024 Apr 2. doi: 10.1007/s10126-024-10306-y. Online ahead of print.NO ABSTRACTPMID:38561552 | DOI:10.1007/s10126-024-10306-y (Source: Marine Biotechnology)
Source: Marine Biotechnology - April 1, 2024 Category: Biotechnology Authors: Insaf Naser Yusuke Yabu Yoshiaki Maeda Tsuyoshi Tanaka Source Type: research
Production of Antioxidant, Angiotensin-Converting Enzyme Inhibitory and Osteogenic Gelatin Hydrolysate from Labeo rohita Swim Bladder
Mar Biotechnol (NY). 2024 Apr 1. doi: 10.1007/s10126-024-10305-z. Online ahead of print.ABSTRACTOptimization of antioxidants and angiotensin-converting enzyme (ACE) inhibitory potential gelatin hydrolysate production from Labeo rohita (rohu) swim bladder (SBGH) by alcalase using central composite design (CCD) of response surface methodology (RSM) was investigated. The maximum degree of hydrolysis (DH), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), total antioxidants (TAO), and ACE inhibitory activity were achieved at 0.1:1.0 (w/w) enzyme to substrate ratio, 61 °C hydro...
Source: Marine Biotechnology - April 1, 2024 Category: Biotechnology Authors: Balaji Wamanrao Kanwate Kalpana Patel Sandesh Suresh Karkal Deependra Rajoriya Kunal Sharan Tanaji G Kudre Source Type: research
Correction: Highly Efficient Genetic Transformation Methods for the Marine Oleaginous Diatom Fistulifera solaris
Mar Biotechnol (NY). 2024 Apr 2. doi: 10.1007/s10126-024-10306-y. Online ahead of print.NO ABSTRACTPMID:38561552 | DOI:10.1007/s10126-024-10306-y (Source: Marine Biotechnology)
Source: Marine Biotechnology - April 1, 2024 Category: Biotechnology Authors: Insaf Naser Yusuke Yabu Yoshiaki Maeda Tsuyoshi Tanaka Source Type: research
Production of Antioxidant, Angiotensin-Converting Enzyme Inhibitory and Osteogenic Gelatin Hydrolysate from Labeo rohita Swim Bladder
Mar Biotechnol (NY). 2024 Apr 1. doi: 10.1007/s10126-024-10305-z. Online ahead of print.ABSTRACTOptimization of antioxidants and angiotensin-converting enzyme (ACE) inhibitory potential gelatin hydrolysate production from Labeo rohita (rohu) swim bladder (SBGH) by alcalase using central composite design (CCD) of response surface methodology (RSM) was investigated. The maximum degree of hydrolysis (DH), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), total antioxidants (TAO), and ACE inhibitory activity were achieved at 0.1:1.0 (w/w) enzyme to substrate ratio, 61 °C hydro...
Source: Marine Biotechnology - April 1, 2024 Category: Biotechnology Authors: Balaji Wamanrao Kanwate Kalpana Patel Sandesh Suresh Karkal Deependra Rajoriya Kunal Sharan Tanaji G Kudre Source Type: research
Correction: Highly Efficient Genetic Transformation Methods for the Marine Oleaginous Diatom Fistulifera solaris
Mar Biotechnol (NY). 2024 Apr 2. doi: 10.1007/s10126-024-10306-y. Online ahead of print.NO ABSTRACTPMID:38561552 | DOI:10.1007/s10126-024-10306-y (Source: Marine Biotechnology)
Source: Marine Biotechnology - April 1, 2024 Category: Biotechnology Authors: Insaf Naser Yusuke Yabu Yoshiaki Maeda Tsuyoshi Tanaka Source Type: research
Production of Antioxidant, Angiotensin-Converting Enzyme Inhibitory and Osteogenic Gelatin Hydrolysate from Labeo rohita Swim Bladder
Mar Biotechnol (NY). 2024 Apr 1. doi: 10.1007/s10126-024-10305-z. Online ahead of print.ABSTRACTOptimization of antioxidants and angiotensin-converting enzyme (ACE) inhibitory potential gelatin hydrolysate production from Labeo rohita (rohu) swim bladder (SBGH) by alcalase using central composite design (CCD) of response surface methodology (RSM) was investigated. The maximum degree of hydrolysis (DH), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), total antioxidants (TAO), and ACE inhibitory activity were achieved at 0.1:1.0 (w/w) enzyme to substrate ratio, 61 °C hydro...
Source: Marine Biotechnology - April 1, 2024 Category: Biotechnology Authors: Balaji Wamanrao Kanwate Kalpana Patel Sandesh Suresh Karkal Deependra Rajoriya Kunal Sharan Tanaji G Kudre Source Type: research
