Real-world performance of the NeuMoDx ™ HCV Quant Test for quantification of hepatitis C virus (HCV)-RNA
J Virol Methods. 2024 Apr 11:114937. doi: 10.1016/j.jviromet.2024.114937. Online ahead of print.ABSTRACTQuantification of hepatitis C virus (HCV)-RNA in serum or plasma samples is an essential parameter in HCV diagnostics. Here, the NeuMoDx™Molecular System (Qiagen) was tested for the most common HCV genotypes and compared to the cobas c6800 system (Roche). HCV-RNA from 131 plasma/serum samples from chronically infected patients was determined in parallel on the NeuMoDx and c6800 systems. Linearity was analysed using the four most common HCV genotypes (1-4) in our cohort. The coefficient of variation (CV) within (intra-a...
Source: Journal of Virological Methods - April 13, 2024 Category: Virology Authors: Nadine L übke Andreas Walker Martin Obermeier Jennifer Camdereli Martha Paluschinski Lara Walotka Anna-Kathrin Schupp Inga Tometten Sandra Hauka Eva Heger J örg Timm Source Type: research
Clinical validation of SARS-CoV-2 electrochemical immunosensor based on the spike-ACE2 complex
CONCLUSIONS: It demonstrates the potential of electrochemical biosensors to be implemented as highly sensitive and easily deployable detection strategies even in remote locations.PMID:38608761 | DOI:10.1016/j.jviromet.2024.114940 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - April 12, 2024 Category: Virology Authors: Viviana V ásquez Jahir Orozco Source Type: research
A Validated In-House Assay for HIV Drug Resistance Mutation Surveillance from Dried Blood Spot Specimens
J Virol Methods. 2024 Apr 9:114939. doi: 10.1016/j.jviromet.2024.114939. Online ahead of print.ABSTRACTDespite increasing scale-up of antiretroviral therapy (ART) coverage, challenges related to adherence and HIV drug resistance (HIVDR) remain. The high cost of HIVDR surveillance is a persistent challenge with implementation in resource-constrained settings. Dried blood spot (DBS) specimens have been demonstrated to be a feasible alternative to plasma or serum for HIVDR genotyping and are more suitable for lower resource settings. There is a need for affordable HIVDR genotyping assays which can amplify HIV-1 sequences from...
Source: Journal of Virological Methods - April 11, 2024 Category: Virology Authors: Bronwyn Neufeld Chantal Munyuza Alexandria Reimer Rupert Capi ña Emma R Lee Marissa Becker Paul Sandstrom Hezhao Ji Fran çois Cholette Source Type: research
A Validated In-House Assay for HIV Drug Resistance Mutation Surveillance from Dried Blood Spot Specimens
J Virol Methods. 2024 Apr 9:114939. doi: 10.1016/j.jviromet.2024.114939. Online ahead of print.ABSTRACTDespite increasing scale-up of antiretroviral therapy (ART) coverage, challenges related to adherence and HIV drug resistance (HIVDR) remain. The high cost of HIVDR surveillance is a persistent challenge with implementation in resource-constrained settings. Dried blood spot (DBS) specimens have been demonstrated to be a feasible alternative to plasma or serum for HIVDR genotyping and are more suitable for lower resource settings. There is a need for affordable HIVDR genotyping assays which can amplify HIV-1 sequences from...
Source: Journal of Virological Methods - April 11, 2024 Category: Virology Authors: Bronwyn Neufeld Chantal Munyuza Alexandria Reimer Rupert Capi ña Emma R Lee Marissa Becker Paul Sandstrom Hezhao Ji Fran çois Cholette Source Type: research
Development and analytical validation of a novel quantitative PCR assay for the detection of Trachemys herpesvirus 1
This study aimed to develop a sensitive and specific quantitative real-time PCR (qPCR) assay for the detection of TrHV1 DNA to aid in the characterization of the epidemiology of this virus in aquatic turtles. Two TaqMan-MGB FAM-dye labeled primer-probe sets were designed and evaluated using plasmid dilutions. The higher performing assay was specific for TrHV1 DNA and had a linear dynamic range of 1.0 × 107 to 1.0 × 101 copies per reaction with an R2 of 0.999, slope of -3.386, and efficiency of 97.39%. The limit of detection was 101 copies per reaction, and there was no loss of reaction efficiency in the presence of TrHV1...
Source: Journal of Virological Methods - April 10, 2024 Category: Virology Authors: Kaitlin A Moorhead Laura A Adamovicz Matthew C Allender Source Type: research
Development and analytical validation of a novel quantitative PCR assay for the detection of Trachemys herpesvirus 1
This study aimed to develop a sensitive and specific quantitative real-time PCR (qPCR) assay for the detection of TrHV1 DNA to aid in the characterization of the epidemiology of this virus in aquatic turtles. Two TaqMan-MGB FAM-dye labeled primer-probe sets were designed and evaluated using plasmid dilutions. The higher performing assay was specific for TrHV1 DNA and had a linear dynamic range of 1.0 × 107 to 1.0 × 101 copies per reaction with an R2 of 0.999, slope of -3.386, and efficiency of 97.39%. The limit of detection was 101 copies per reaction, and there was no loss of reaction efficiency in the presence of TrHV1...
Source: Journal of Virological Methods - April 10, 2024 Category: Virology Authors: Kaitlin A Moorhead Laura A Adamovicz Matthew C Allender Source Type: research
RSV-GenoScan: an automated pipeline for whole-genome human respiratory syncytial virus (RSV) sequence analysis
We present RSV-GenoScan, a fast and easy-to-use pipeline for WGS analysis of RSV generated by HTS on Illumina or Nanopore platforms. RSV-GenoScan automates the WGS analysis steps directly from the raw sequence data. The pipeline filters the sequence data, maps the reads to the RSV reference genome, generates a consensus sequence, identifies the RSV subgroup, and lists amino acid mutations, insertions and deletions in the F and G viral genes. This enables the rapid identification of mutations in these coding genes that are known to confer resistance to monoclonal antibodies.AVAILABILITY: RSV-GenoScan is freely available at ...
Source: Journal of Virological Methods - April 8, 2024 Category: Virology Authors: Alexandre Dosbaa Romane Guilbaud Anna-Maria Franco Yusti Valentine Marie Ferr é Charlotte Charpentier Diane Descamps Quentin Le Hingrat Romain Copp ée Source Type: research
RSV-GenoScan: an automated pipeline for whole-genome human respiratory syncytial virus (RSV) sequence analysis
We present RSV-GenoScan, a fast and easy-to-use pipeline for WGS analysis of RSV generated by HTS on Illumina or Nanopore platforms. RSV-GenoScan automates the WGS analysis steps directly from the raw sequence data. The pipeline filters the sequence data, maps the reads to the RSV reference genome, generates a consensus sequence, identifies the RSV subgroup, and lists amino acid mutations, insertions and deletions in the F and G viral genes. This enables the rapid identification of mutations in these coding genes that are known to confer resistance to monoclonal antibodies.AVAILABILITY: RSV-GenoScan is freely available at ...
Source: Journal of Virological Methods - April 8, 2024 Category: Virology Authors: Alexandre Dosbaa Romane Guilbaud Anna-Maria Franco Yusti Valentine Marie Ferr é Charlotte Charpentier Diane Descamps Quentin Le Hingrat Romain Copp ée Source Type: research
RSV-GenoScan: an automated pipeline for whole-genome human respiratory syncytial virus (RSV) sequence analysis
We present RSV-GenoScan, a fast and easy-to-use pipeline for WGS analysis of RSV generated by HTS on Illumina or Nanopore platforms. RSV-GenoScan automates the WGS analysis steps directly from the raw sequence data. The pipeline filters the sequence data, maps the reads to the RSV reference genome, generates a consensus sequence, identifies the RSV subgroup, and lists amino acid mutations, insertions and deletions in the F and G viral genes. This enables the rapid identification of mutations in these coding genes that are known to confer resistance to monoclonal antibodies.AVAILABILITY: RSV-GenoScan is freely available at ...
Source: Journal of Virological Methods - April 8, 2024 Category: Virology Authors: Alexandre Dosbaa Romane Guilbaud Anna-Maria Franco Yusti Valentine Marie Ferr é Charlotte Charpentier Diane Descamps Quentin Le Hingrat Romain Copp ée Source Type: research
Insertion of foreign genes into the simian varicella virus genome by Tn7-mediated site-specific transposition
J Virol Methods. 2024 Apr 5:114936. doi: 10.1016/j.jviromet.2024.114936. Online ahead of print.ABSTRACTA Tn7-transposition approach was utilized for site-specific insertion of foreign genes into the genome of simian varicella virus (SVV), the causative agent of simian varicella in nonhuman primates. The severe acute respiratory syndrome coronavirus (SARS-CoV-2) nucleocapsid (N) gene and receptor binding domain (RBD) of the spike gene were inserted into the ORF 14 region of the SVV genome cloned into a bacterial artificial chromosome and then transfected into Vero cells to generate the infectious recombinant SVV (rSVV). The...
Source: Journal of Virological Methods - April 7, 2024 Category: Virology Authors: Wayne L Gray Source Type: research
Intracellular localized heterogeneous protein franking by a transmembrane domain of GP64 is sufficient to be assembled on budded virions of Bombyx mori nucleopolyhedrovirus
In this study, we further investigate and develop this BV surface displaying strategy. We constructed stable insect cell lines to express the target protein flanking with different regions of signal peptide (SP) and GP64 transmembrane domain (TMD). Subsequently, recombinant BmNPV was used to infect the cell, and the integration of heterogeneous protein into BV was detected. The results indicated that deletion of the n-region of SP (SPΔn) decreased the incorporation rate more than that of the full-length SP. However, the incorporation rate of the protein fused with h and c-region deletion of SP (SPΔh-c) was significantly ...
Source: Journal of Virological Methods - April 6, 2024 Category: Virology Authors: Yufeng Hao Na Liu Jingfeng Li Stephen Baffour Gyawu Ogone Emeldah Setshogo Jinshan Huang Bifang Hao Source Type: research
Construction and characterization of recombinant senecavirus A expressing secreted luciferase for antiviral screening
In this study, we successfully constructed a recombinant SVA expressing Gaussia Luciferase (Gluc), termed rSVA-Gluc. The growth kinetics of rSVA-Gluc in BHK-21 cells were found to be comparable to those of the parental virus, and Gluc activity paralleled the virus growth curve. Genetic analysis revealed stable inheritance of the inserted reporter protein genes for at least six generations. We evaluated the utility of rSVA-Gluc in antiviral drug screening, and the results highlighted its potential as an effective tool for such purposes against SVA. DATA AVAILABILITY STATEMENT: The data that support the findings of this stud...
Source: Journal of Virological Methods - April 6, 2024 Category: Virology Authors: Hao Wang Yongfang Mo Wenbo Liu Qijie He Tongwei Ren Kang Ouyang Ying Chen Weijian Huang Zuzhang Wei Source Type: research
Intracellular localized heterogeneous protein franking by a transmembrane domain of GP64 is sufficient to be assembled on budded virions of Bombyx mori nucleopolyhedrovirus
In this study, we further investigate and develop this BV surface displaying strategy. We constructed stable insect cell lines to express the target protein flanking with different regions of signal peptide (SP) and GP64 transmembrane domain (TMD). Subsequently, recombinant BmNPV was used to infect the cell, and the integration of heterogeneous protein into BV was detected. The results indicated that deletion of the n-region of SP (SPΔn) decreased the incorporation rate more than that of the full-length SP. However, the incorporation rate of the protein fused with h and c-region deletion of SP (SPΔh-c) was significantly ...
Source: Journal of Virological Methods - April 6, 2024 Category: Virology Authors: Yufeng Hao Na Liu Jingfeng Li Stephen Baffour Gyawu Ogone Emeldah Setshogo Jinshan Huang Bifang Hao Source Type: research
Construction and characterization of Recombinant Senecavirus A Expressing Secreted Luciferase for antiviral screening
In this study, we successfully constructed a recombinant SVA expressing Gaussia Luciferase (Gluc), termed rSVA-Gluc. The growth kinetics of rSVA-Gluc in BHK-21 cells were found to be comparable to those of the parental virus, and Gluc activity paralleled the virus growth curve. Genetic analysis revealed stable inheritance of the inserted reporter protein genes for at least six generations. We evaluated the utility of rSVA-Gluc in antiviral drug screening, and the results highlighted its potential as an effective tool for such purposes against SVA. DATA AVAILABILITY STATEMENT: The data that support the findings of this stud...
Source: Journal of Virological Methods - April 6, 2024 Category: Virology Authors: Hao Wang Yongfang Mo Wenbo Liu Qijie He Tongwei Ren Kang Ouyang Ying Chen Weijian Huang Zuzhang Wei Source Type: research
Evaluation of an automated platform for the detection of HEV RNA in plasma and stool
CONCLUSIONS: The Altostar platform enables highly accurate testing for the detection of HEV RNA in stool and the quantification of HEV RNA in plasma. This allowed us to shorten turnaround times and to save time for the technical staff.PMID:38574772 | DOI:10.1016/j.jviromet.2024.114920 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - April 4, 2024 Category: Virology Authors: Pauline Sottil S ébastien Lhomme Karine Saune Soheil El Hayani K évin Oliveira-Mendes Jean-Marie Peron Nassim Kamar Jacques Izopet Florence Abravanel Source Type: research
