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Inhibition of Verticillium Wilt in Cotton through the Application of Pseudomonas aeruginosa ZL6 Derived from Fermentation Residue of Kitchen Waste
J Microbiol Biotechnol. 2024 Apr 5;34(5):1-10. doi: 10.4014/jmb.2401.01022. Online ahead of print.ABSTRACTTo isolate and analyze bacteria with Verticillium wilt-resistant properties from the fermentation residue of kitchen wastes, as well as explore their potential for new applications of the residue. A total of six bacterial strains exhibiting Verticillium wilt-resistant capabilities were isolated from the biogas residue of kitchen waste fermentation. Using a polyphasic approach, strain ZL6, which displayed the highest antagonistic activity against cotton Verticillium wilt, was identified as belonging to the Pseudomonas a...
Source: Journal of Microbiology and Biotechnology - April 11, 2024 Category: Biotechnology Authors: Qiuhong Niu Shengwei Lei Guo Zhang Guohan Wu Zhuo Tian Keyan Chen Lin Zhang Source Type: research

In vivo Effects of Salicornia herbacea and Calystegia soldanella Extracts for Memory Improvement
J Microbiol Biotechnol. 2024 Mar 13;34(5):1-10. doi: 10.4014/jmb.2312.12045. Online ahead of print.ABSTRACTThe global elderly population, aged 65 and over, reached approximately 10% in 2020, and this proportion is expected to continue rising. Therefore, the prevalence of neurodegenerative diseases such as Parkinson's disease (PD), which are characterized by declining memory capabilities, is anticipated to increase. In a previous study, we successfully restored the diminished memory capabilities in a fruit fly model of PD by administering an omija extract. To identify functional ingredients that can enhance memory akin to t...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Jiun Sang Seeta Poudel Youngseok Lee Source Type: research

 Escherichia coli Cytoplasmic Expression of Disulfide-Bonded Proteins: Side-by-Side Comparison Between Two Competing Strategies
J Microbiol Biotechnol. 2024 Mar 29;34(5):1-10. doi: 10.4014/jmb.2311.11025. Online ahead of print.ABSTRACTThe production of disulfide bond-containing recombinant proteins in Escherichia coli has traditionally been done by either refolding from inclusion bodies or by targeting the protein to the periplasm. However, both approaches have limitations and were developed to allow the production of proteins with disulfide bonds in the cytoplasm of E. coli: i) engineered strains with deletions in the disulfide reduction pathways, e.g. SHuffle, and ii) the co-expression of oxidative folding catalysts, e.g. CyDisCo. However, to our...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Angel Castillo-Corujo Yuko Uchida Mirva J Saaranen Lloyd W Ruddock Source Type: research

Biosynthesis of apigenin glucosides in engineered Corynebacterium glutamicum 
J Microbiol Biotechnol. 2024 Mar 14;34(5):1-10. doi: 10.4014/jmb.2401.01017. Online ahead of print.ABSTRACTGlucosylation is a well-known approach to improve the solubility, pharmacological, and biological properties of flavonoids, making flavonoid glucosides a target for large-scale biosynthesis. However, the low yield of products coupled with the requirement of expensive UDP-sugars limits the application of enzymatic systems for large-scale. C. glutamicum is a Gram-positive and generally regarded as safe (GRAS) bacteria frequently employed for the large-scale production of amino acids and bio-fuels. Due to the versatility...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Obed Jackson Amoah Samir Bahadur Thapa Su Yeong Ma Hue Thi Nguyen Morshed Md Zakaria Jae Kyung Sohng Source Type: research

Construction of a new Agrobacterium tumefaciens -mediated transformation system based on a dual auxotrophic approach in Cordyceps militaris 
In this study, we constructed an ATMT system based on a dual pyrG and hisB auxotrophic mutant of C. militaris. Using the uridine/uracil auxotrophic mutant as the background and pyrG as a selection marker, the hisB gene encoding imidazole glycerophosphate dehydratase, required for histidine biosynthesis, was knocked out by homologous recombination to construct a histidine auxotrophic C. militaris mutant. Then, pyrG in the histidine auxotrophic mutant was deleted to construct a ΔpyrG ΔhisB dual auxotrophic mutant. Further, we established an ATMT transformation system based on the dual auxotrophic C. militaris by using GFP ...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Huan Huan Yan Yi Tong Shang Li Hong Wang Xue Qin Tian Van-Tuan Tran Li Hua Yao Bin Zeng Zhi Hong Hu Source Type: research

Screening Plant Growth-Promoting Bacteria with Antimicrobial Properties for Upland Rice
In conclusion, the in vitro plant growth-promoting and disease-controlling attributes of Bacillus sp. KK281 make it a strong candidate for field evaluation to boost plant growth and manage disease in upland rice.PMID:38563101 | DOI:10.4014/jmb.2402.02008 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Khammool Khamsuk Bernard Dell Wasu Pathom-Aree Wanwarang Pathaichindachote Nungruthai Suphrom Nareeluk Nakaew Juangjun Jumpathong Source Type: research

Improved Thermal Stability of a Novel Acidophilic Phytase
J Microbiol Biotechnol. 2024 Apr 3;34(5):1-10. doi: 10.4014/jmb.2311.11044. Online ahead of print.ABSTRACTPhytase increases the availability of phosphate and trace elements by hydrolyzing the phospho-monoester bond in phytate present in animal feed. It is also an important enzyme from an environmental perspective because it not only promotes the growth of livestocks but also prevents phosphorus contamination released into the environment. Here we present a novel phytase derived from Turicimonas muris, TmPhy, which has distinctive structure and properties compared to other previously known phytases. TmPhy gene expressed in ...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Byung Sam Son So Hyeong Kim Hye-Young Sagong Su Rin Lee Eun Jung Choi Source Type: research

Metabolic regulation of longevity and immune response in Caenorhabditis elegans by ingestion of Lacticaseibacillus rhamnosus IDCC 3201 using multi-omics analysis
In conclusion, this study demonstrated the probiotic properties of the candidate probiotic L. rhamnosus IDCC 3201 by using multi-omics analysis.PMID:38563104 | DOI:10.4014/jmb.2402.02025 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Daniel Junpyo Lee Ju Young Eor Min-Jin Kwak Junbeom Lee An Na Kang Daye Mun Hyejin Choi Minho Song Jong Nam Kim Jun-Mo Kim Jungwoo Yang Hyung Wook Kim Sangnam Oh Younghoon Kim Source Type: research

Plant-Based Decellularization: A Novel Approach for Perfusion-Compatible Tissue Engineering Structures
This study explores the potential of plant-based decellularization in regenerative medicine, a pivotal development in tissue engineering focusing on scaffold development, modification, and vascularization. Plant decellularization involves removing cellular components from plant structures, offering an eco-friendly and cost-effective alternative to traditional scaffold materials. The use of plant-derived polymers is critical, presenting both benefits and challenges, notably in mechanical properties. Integration of plant vascular networks represents a significant bioengineering breakthrough, aligning with natural design prin...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Md Mehedee Hasan Ashikur Rahman Swapon Tazrin Islam Dipti Yeong-Jin Choi Hee-Gyeong Yi Source Type: research

The use of the internal transcribed spacer region for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei infecting whiteleg shrimp (Penaeus vannamei) and for the development of a nested PCR as its diagnostic tool
In this study, the ITS-1 region sequence (~600-bp) of EHP was first identified, and primers for a polymerase chain reaction (PCR) assay targeting that sequence were designed. A newly developed nested-PCR method successfully detected the EHP in various shrimp (Penaeus vannamei and P. monodon) and related samples, including water and feces collected from Indonesia, Thailand, South Korea, India, and Malaysia. The primers did not cross-react with other hosts and pathogens, and this PCR assay is more sensitive than existing PCR detection methods targeting the small subunit ribosomal RNA (SSU rRNA) and spore wall protein (SWP) g...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Ju Hee Lee Hye Jin Jeon Sangsu Seo Chorong Lee Bumkeun Kim Dong-Mi Kwak Man Hee Rhee Patharapol Piamsomboon Yani Lestari Nuraini Chang Uook Je Seon Young Park Ji Hyung Kim Jee Eun Han Source Type: research

Genetic Characteristics of Extended-Spectrum Beta-Lactamase-Producing Salmonella Isolated from Retail Meats in South Korea
J Microbiol Biotechnol. 2024 Mar 22;34(5):1-10. doi: 10.4014/jmb.2312.12018. Online ahead of print.ABSTRACTEarlier studies have validated the isolation of extended-spectrum beta-lactamase-producing Salmonella (ESBL-Sal) strains from food. While poultry is recognized as a reservoir for Salmonella contamination, pertinent data regarding ESBL-Sal remains limited. Consequently, the Ministry of Food and Drug Safety has isolated Salmonella spp. from retail meat and evaluated their antibiotic susceptibility and genetic characteristics via whole-genome sequencing. To further elucidate these aspects, this study investigates the pre...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Haiseong Kang Hansol Kim Hyochin Kim Ji Hye Jeon Seokhwan Kim Yongchjun Park Soon Han Kim Source Type: research