Stress resistant rpsU variants of Listeria monocytogenes can become underrepresented due to enrichment bias
This study investigated whether these switches in phenotypes could result in a bias in strain detection when standard enrichment-based procedures are applied to a variety of strains. Detailed growth kinetics analysis of L. monocytogenes strains were performed, including the LO28 wild type (WT) and rpsU variants V14 and V15, during two commonly used enrichment-based procedures described in the ISO 11290-1:2017 and the U.S. Food and Drug Administration Bacteriological Analytical Manual (BAM). WT had a higher growth rate than the variants during the enrichment processes. Co-culture growth kinetics predictions for WT and rpsU ...
Source: International Journal of Food Microbiology - March 24, 2024 Category: Food Science Authors: Xuchuan Ma Jingjie Chen Marcel H Zwietering Tjakko Abee Heidy M W Den Besten Source Type: research

Stress resistant rpsU variants of Listeria monocytogenes can become underrepresented due to enrichment bias
This study investigated whether these switches in phenotypes could result in a bias in strain detection when standard enrichment-based procedures are applied to a variety of strains. Detailed growth kinetics analysis of L. monocytogenes strains were performed, including the LO28 wild type (WT) and rpsU variants V14 and V15, during two commonly used enrichment-based procedures described in the ISO 11290-1:2017 and the U.S. Food and Drug Administration Bacteriological Analytical Manual (BAM). WT had a higher growth rate than the variants during the enrichment processes. Co-culture growth kinetics predictions for WT and rpsU ...
Source: International Journal of Food Microbiology - March 24, 2024 Category: Food Science Authors: Xuchuan Ma Jingjie Chen Marcel H Zwietering Tjakko Abee Heidy M W Den Besten Source Type: research

Substitution of Asp29 with Asn29 in the metallochaperone UreE of Streptococcus thermophilus DSM 20617 < sup > T < /sup > increases the urease activity and anticipates urea hydrolysis during milk fermentation
Int J Food Microbiol. 2024 Mar 16;416:110684. doi: 10.1016/j.ijfoodmicro.2024.110684. Online ahead of print.ABSTRACTUrease operon is highly conserved within the species Streptococcus thermophilus and urease-negative strains are rare in nature. S. thermophilus MIMO1, isolated from commercial yogurt, was previously characterized as urease-positive Ni-dependent strain. Beside a mutation in ureQ, coding for a nickel ABC transporter permease, the strain MIMO1 showed a mutation in ureE gene which code for a metallochaperone involved in Ni delivery to the urease catalytic site. The single base mutation in ureE determined a substi...
Source: International Journal of Food Microbiology - March 21, 2024 Category: Food Science Authors: Stefania Arioli Nicola Mangieri Ylenia Zanchetta Pasquale Russo Diego Mora Source Type: research

Substitution of Asp29 with Asn29 in the metallochaperone UreE of Streptococcus thermophilus DSM 20617 < sup > T < /sup > increases the urease activity and anticipates urea hydrolysis during milk fermentation
Int J Food Microbiol. 2024 Mar 16;416:110684. doi: 10.1016/j.ijfoodmicro.2024.110684. Online ahead of print.ABSTRACTUrease operon is highly conserved within the species Streptococcus thermophilus and urease-negative strains are rare in nature. S. thermophilus MIMO1, isolated from commercial yogurt, was previously characterized as urease-positive Ni-dependent strain. Beside a mutation in ureQ, coding for a nickel ABC transporter permease, the strain MIMO1 showed a mutation in ureE gene which code for a metallochaperone involved in Ni delivery to the urease catalytic site. The single base mutation in ureE determined a substi...
Source: International Journal of Food Microbiology - March 21, 2024 Category: Food Science Authors: Stefania Arioli Nicola Mangieri Ylenia Zanchetta Pasquale Russo Diego Mora Source Type: research

Substitution of Asp29 with Asn29 in the metallochaperone UreE of Streptococcus thermophilus DSM 20617 < sup > T < /sup > increases the urease activity and anticipates urea hydrolysis during milk fermentation
Int J Food Microbiol. 2024 Mar 16;416:110684. doi: 10.1016/j.ijfoodmicro.2024.110684. Online ahead of print.ABSTRACTUrease operon is highly conserved within the species Streptococcus thermophilus and urease-negative strains are rare in nature. S. thermophilus MIMO1, isolated from commercial yogurt, was previously characterized as urease-positive Ni-dependent strain. Beside a mutation in ureQ, coding for a nickel ABC transporter permease, the strain MIMO1 showed a mutation in ureE gene which code for a metallochaperone involved in Ni delivery to the urease catalytic site. The single base mutation in ureE determined a substi...
Source: International Journal of Food Microbiology - March 21, 2024 Category: Food Science Authors: Stefania Arioli Nicola Mangieri Ylenia Zanchetta Pasquale Russo Diego Mora Source Type: research

The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol
This study explores the impact of different acidic conditions of culture media and food matrices on the development and removal of biofilms developed on stainless steel surfaces by wild-type (WT) L. monocytogenes strains as well as in two mutant derivatives, ΔsigB and ΔagrA, that have defects in the general stress response and quorum sensing, respectively. Additionally, the study investigates the efficacy of nanoencapsulated carvacrol as an antimicrobial against L. monocytogenes biofilms developed in Tryptic Soy Broth (TSB) culture media acidified to different pH conditions (3.5, 4.5, 5.5, 6.5), and in food substrates (a...
Source: International Journal of Food Microbiology - March 20, 2024 Category: Food Science Authors: Jina Yammine Agapi I Doulgeraki Conor P O'Byrne Adem Gharsallaoui Nour-Eddine Chihib Layal Karam Source Type: research

The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol
This study explores the impact of different acidic conditions of culture media and food matrices on the development and removal of biofilms developed on stainless steel surfaces by wild-type (WT) L. monocytogenes strains as well as in two mutant derivatives, ΔsigB and ΔagrA, that have defects in the general stress response and quorum sensing, respectively. Additionally, the study investigates the efficacy of nanoencapsulated carvacrol as an antimicrobial against L. monocytogenes biofilms developed in Tryptic Soy Broth (TSB) culture media acidified to different pH conditions (3.5, 4.5, 5.5, 6.5), and in food substrates (a...
Source: International Journal of Food Microbiology - March 20, 2024 Category: Food Science Authors: Jina Yammine Agapi I Doulgeraki Conor P O'Byrne Adem Gharsallaoui Nour-Eddine Chihib Layal Karam Source Type: research

Molecular characterization of antibiotic resistant Salmonella enterica across the poultry production chain in Costa Rica: A cross-sectional study
Int J Food Microbiol. 2024 Mar 7;416:110663. doi: 10.1016/j.ijfoodmicro.2024.110663. Online ahead of print.ABSTRACTAntibiotic resistant Salmonella enterica are on the increase, worldwide. Given the scarcity of data, this study aimed to investigate its occurrence, virulence, and antibiotic resistance in Costa Rica's food chain. In total, 65 chicken meat- and 171 chicken caecal samples were collected and examined for Salmonella. High frequencies of Salmonella were found in chicken meat (58.5 %, n/N = 38/65) and poultry farms (38.0 %, n/N = 65/171). The majority of Salmonella from chicken meat (89.5 %, n/N = 34/38) and caecum...
Source: International Journal of Food Microbiology - March 19, 2024 Category: Food Science Authors: Andrea Molina Thorsten Thye Lohendy Mu ñoz-Vargas Rebeca Zamora-Sanabria Daniel Haile Chercos Raquel Hern ández-Rojas Noelia Robles Daniela Aguilar J ürgen May Denise Dekker Source Type: research

Molecular characterization of antibiotic resistant Salmonella enterica across the poultry production chain in Costa Rica: A cross-sectional study
Int J Food Microbiol. 2024 Mar 7;416:110663. doi: 10.1016/j.ijfoodmicro.2024.110663. Online ahead of print.ABSTRACTAntibiotic resistant Salmonella enterica are on the increase, worldwide. Given the scarcity of data, this study aimed to investigate its occurrence, virulence, and antibiotic resistance in Costa Rica's food chain. In total, 65 chicken meat- and 171 chicken caecal samples were collected and examined for Salmonella. High frequencies of Salmonella were found in chicken meat (58.5 %, n/N = 38/65) and poultry farms (38.0 %, n/N = 65/171). The majority of Salmonella from chicken meat (89.5 %, n/N = 34/38) and caecum...
Source: International Journal of Food Microbiology - March 19, 2024 Category: Food Science Authors: Andrea Molina Thorsten Thye Lohendy Mu ñoz-Vargas Rebeca Zamora-Sanabria Daniel Haile Chercos Raquel Hern ández-Rojas Noelia Robles Daniela Aguilar J ürgen May Denise Dekker Source Type: research

Evaluation of a new automated viral RNA extraction platform for hepatitis A virus and human norovirus in testing of berries, lettuce, and oysters
Int J Food Microbiol. 2024 Mar 7;416:110664. doi: 10.1016/j.ijfoodmicro.2024.110664. Online ahead of print.ABSTRACTFruits, vegetables, and shellfish are often associated with outbreaks of illness caused particularly by human norovirus (HuNoV) and hepatitis A virus (HAV), the leading causative agents of foodborne illness worldwide. The aim of this study was to evaluate a new automated nucleic acid extraction platform (EGENE-UP EASYPREP) for enteric viruses in several at-risk food matrices and to test its limit of detection in comparison to a semi-automated method (EGENE-UP) using Boom methodology for nucleic acid extraction...
Source: International Journal of Food Microbiology - March 16, 2024 Category: Food Science Authors: Mathilde Trudel-Ferland Marie- Ève Collard Val érie Goulet-Beaulieu Eric Jubinville Fabienne Hamon Julie Jean Source Type: research

Evaluation of a new automated viral RNA extraction platform for hepatitis A virus and human norovirus in testing of berries, lettuce, and oysters
Int J Food Microbiol. 2024 Mar 7;416:110664. doi: 10.1016/j.ijfoodmicro.2024.110664. Online ahead of print.ABSTRACTFruits, vegetables, and shellfish are often associated with outbreaks of illness caused particularly by human norovirus (HuNoV) and hepatitis A virus (HAV), the leading causative agents of foodborne illness worldwide. The aim of this study was to evaluate a new automated nucleic acid extraction platform (EGENE-UP EASYPREP) for enteric viruses in several at-risk food matrices and to test its limit of detection in comparison to a semi-automated method (EGENE-UP) using Boom methodology for nucleic acid extraction...
Source: International Journal of Food Microbiology - March 16, 2024 Category: Food Science Authors: Mathilde Trudel-Ferland Marie- Ève Collard Val érie Goulet-Beaulieu Eric Jubinville Fabienne Hamon Julie Jean Source Type: research

Evaluation of a new automated viral RNA extraction platform for hepatitis A virus and human norovirus in testing of berries, lettuce, and oysters
Int J Food Microbiol. 2024 Mar 7;416:110664. doi: 10.1016/j.ijfoodmicro.2024.110664. Online ahead of print.ABSTRACTFruits, vegetables, and shellfish are often associated with outbreaks of illness caused particularly by human norovirus (HuNoV) and hepatitis A virus (HAV), the leading causative agents of foodborne illness worldwide. The aim of this study was to evaluate a new automated nucleic acid extraction platform (EGENE-UP EASYPREP) for enteric viruses in several at-risk food matrices and to test its limit of detection in comparison to a semi-automated method (EGENE-UP) using Boom methodology for nucleic acid extraction...
Source: International Journal of Food Microbiology - March 16, 2024 Category: Food Science Authors: Mathilde Trudel-Ferland Marie- Ève Collard Val érie Goulet-Beaulieu Eric Jubinville Fabienne Hamon Julie Jean Source Type: research

A consortium of different Saccharomyces species enhances the content of bioactive tryptophan-derived compounds in wine fermentations
This study is the first to demonstrate how the formation of microbial consortia can serve as a useful strategy to enhance compounds with interesting properties in wine, paving the way for future studies and combinations.PMID:38490108 | DOI:10.1016/j.ijfoodmicro.2024.110681 (Source: International Journal of Food Microbiology)
Source: International Journal of Food Microbiology - March 15, 2024 Category: Food Science Authors: Andr és Planells-Cárcel Julia Kazakova Cristina P érez Marina Gonzalez-Ramirez M Carmen Garcia-Parrilla Jos é M Guillamón Source Type: research

Impact of predicted climate change environmental conditions on the growth of Fusarium asiaticum strains and mycotoxins production on a wheat-based matrix
Int J Food Microbiol. 2024 Mar 7;416:110658. doi: 10.1016/j.ijfoodmicro.2024.110658. Online ahead of print.ABSTRACTFusarium asiaticum is a predominant fungal pathogen causing Fusarium Head Blight (FHB) in wheat and barley in China and is associated with approximately £201 million in annual losses due to grains contaminated with mycotoxins. F. asiaticum produces deoxynivalenol and zearalenone whose maximum limits in cereals and cereals-derived products have been established in different countries including the EU. Few studies are available on the ecophysiological behaviour of this fungal pathogen, but nothing is known abou...
Source: International Journal of Food Microbiology - March 14, 2024 Category: Food Science Authors: Carla Cervini Naoreen Naz Carol Verheecke-Vaessen Angel Medina Source Type: research

Impact of predicted climate change environmental conditions on the growth of Fusarium asiaticum strains and mycotoxins production on a wheat-based matrix
Int J Food Microbiol. 2024 Mar 7;416:110658. doi: 10.1016/j.ijfoodmicro.2024.110658. Online ahead of print.ABSTRACTFusarium asiaticum is a predominant fungal pathogen causing Fusarium Head Blight (FHB) in wheat and barley in China and is associated with approximately £201 million in annual losses due to grains contaminated with mycotoxins. F. asiaticum produces deoxynivalenol and zearalenone whose maximum limits in cereals and cereals-derived products have been established in different countries including the EU. Few studies are available on the ecophysiological behaviour of this fungal pathogen, but nothing is known abou...
Source: International Journal of Food Microbiology - March 14, 2024 Category: Food Science Authors: Carla Cervini Naoreen Naz Carol Verheecke-Vaessen Angel Medina Source Type: research