Indian Journal of Clinical Biochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

The Combined Impact of Curcumin: Piperine and Sorafenib on microRNAs and Different Pathways in Breast Cancer Cells
This study suggests that the combination of sorafenib and curcumin: piperine can combat breast cancer by modulating several microRNAs and signaling pathways involved in the development and progression of breast cancer. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - April 18, 2024 Category: Biochemistry Source Type: research

Hypertensive Pregnancy Supports Higher Adaptation of Stress Over Anemic Pregnancy: A Pilot Study
This study targets relative stress adaptation in pregnancy between hypertension and anemia. This study has been conducted on three types of pregnancies viz. regular, hypertensive and anemic pregnancy. The data obtained were compared against normal healthy controls. Bloo d pressure was monitored to label hypertensive pregnancy. Lipid profile was the severity marker for hypertensive subjects. Low hemoglobin and MCV levels were used as marker for anemia. Glycemic profile was verified by plasma glucose concentration to exclude diabetic subjects. BMI was calculated to e xclude obesity. Commercially available ELISA based kits we...
Source: Indian Journal of Clinical Biochemistry - April 17, 2024 Category: Biochemistry Source Type: research

Development of a Robust, Rapid and Reliable Tandem Mass Spectrometry Method for the Measurement of Sildenafil, Bosentan and their Major Metabolites
In this study, we aimed to develop a liquid chromatography-tandem mass spectrometry method (LC –MS/MS) to measure the levels of bosentan, sildenafil, and their active metabolites in patients with PAH. We have developed an LC–MS/MS measurement procedure using a liquid–liquid extraction to measure serum drug concentrations and validated the procedure according to Clinical and Laboratory S tandards Institute (CLSI) protocols.Finally, the validated method was used to measure the levels of sildenafil, bosentan, and their metabolite in pediatric PAH patients. The method was linear in the range of 0.975 –1000 ng/ml and 0...
Source: Indian Journal of Clinical Biochemistry - April 15, 2024 Category: Biochemistry Source Type: research

Thymol Enhances 5-Fluorouracil Cytotoxicity by Reducing Migration and Increasing Apoptosis and Cell Cycle Arrest in Esophageal Cancer Cells: An In-vitro Study
AbstractEsophageal cancer (EC) is a common cancer globally and has a low survival rate because it is often diagnosed at a late-stage, and is frequently chemotherapy resistant. Thymol is found in the herb thyme and has been reported to have potential anticancer properties. We aimed to explore the effects of thymol on the efficacy of 5-Fluorouracil (5-FU) treatment in an in vitro model of EC. We evaluated the synergistic effects of thymol with 5-FU using cell viability, apoptosis, cell cycle analysis, scratch wound healing assay, gelatin zymography, as well as reactive oxygen species (ROS) generation  aproaches. The results...
Source: Indian Journal of Clinical Biochemistry - April 13, 2024 Category: Biochemistry Source Type: research

Differential Expression of Circulating miR-221-3p, miR-146a-5p, miR-206 and Their Diagnostic Value in Lung Cancer
AbstractLung cancer is a major health problem and the second most common cancer in the Kashmiri population. It is often diagnosed at an advanced stage, leading to poor clinical outcomes. The aim of this study was to investigate the differential expression of serum microRNAs (miR-146a-5p, miR-206 and miR-221-3p) and their diagnostic value in lung cancer patients from Kashmir. Serum miRNAs were isolated using a Trizol-based protocol. A pooled reverse transcription protocol was used to convert miRNA to cDNA using miRNA-specific stem-loop primers. Serum miRNA expression was determined by RT-qPCR using Sybr green. miR-221-3p (1...
Source: Indian Journal of Clinical Biochemistry - April 13, 2024 Category: Biochemistry Source Type: research

Association of Interleukins 17A and 17F Gene Polymorphism with Asthma: A Case Control Study in North Indian Population
This study involved 150 cases with asthmatics and 150 healthy controls. Asthmatic pat ients were confirmed by Spirometry and other clinical parameters. The genotype frequencies observed for all genes in controls were in Hardy Weinberg Equilibrium (HWE). The results suggested that the asthmatics of the same height and age had lower weight and higher smoking rate in comparison to contr ols. The asthmatics displayed cough, breathlessness, congestion, disturbed sleep, headache, and wheezing. These parameters were found to be significantly elevated in asthmatics. Excepting Hb, levels of blood eosinophils, AEC, TLC and serum IgE...
Source: Indian Journal of Clinical Biochemistry - April 12, 2024 Category: Biochemistry Source Type: research

The IKAROS Transcription Factor Gene IKZF1 as a Critical Regulator in the Pathogenesis of Childhood Acute Lymphoblastic Leukemia: Insights from a Bangladeshi Population
This study investigated whether two specific IKZF1 variants, rs10272724 (T  >  C) and rs4132601 (T >  G), are potential risk factors for childhood ALL among Bangladeshi children. In this study, we found that ALL occurs with a significantly greater frequency in male children than in female children, shedding light on potential sex-specific susceptibility patterns. Subsequent association analyses u tilizing codominant, dominant, and recessive inheritance models demonstrated that rs10272724 is associated with a 2.36-fold increased risk of ALL development according to the codominant model, a 3.6-fold increased ri...
Source: Indian Journal of Clinical Biochemistry - April 4, 2024 Category: Biochemistry Source Type: research

Hesperidin Supplementation During Alternate Day Fasting Provides Synergistic Effect in Conferring Neuroprotection to Aging Rats
AbstractAlternate day fasting (ADF) is a beneficial dietary intervention that reduces age- associated complications. Hesperidin is a potential antioxidant especially known for free radical scavenging ability. Oxidative damages are a major cause of neurological changes in brain that accelerate deteriorative effects leading to aging. In the current study, we aimed to investigate the role of hesperidin supplementation on possible neuroprotection conferred in terms of redox balance in rats that were maintained on an ADF regimen. Middle aged male Wistar rats (Rattus Norvegicus) (12 –15 months) were divided into four groups: ...
Source: Indian Journal of Clinical Biochemistry - April 4, 2024 Category: Biochemistry Source Type: research

Double Jeopardy!
AbstractCo-presentation of anti GBM and anti MPO antibodies is considered to be a rare phenomena. When patients present both types of antibodies, the condition is referred to as “double positive” for anti-GBM and ANCA associated antibodies which can present clinically as rapidly progressive glomerulonephritis [RPGN], often associated with alveolar bleeding and renal manifestations which constitutes a medical emergency associated with increased morbidity and mortality. I n current clinical practice, the diagnosis is obtained through serological tests that detect the presence of antibodies and kidney biopsy to identify t...
Source: Indian Journal of Clinical Biochemistry - April 3, 2024 Category: Biochemistry Source Type: research

Analysis of Specific Serum Markers for Early Prediction of Alzheimer's Disease in Adolescents with Down Syndrome
This study aimed to uncover early serum marker candidates of amyloid precursor protein-like protein 1 beta species denoted APL1 β25, APL1β27 and APL1β28 and the noradrenergic metabolite 3-methoxy-4-hydroxyphenylglycol (MHPG) as predictors of Alzheimer's disease (AD) in adolescents with DS and to elucidate the correlation between these parameters and the cognition of DS patients. This study included 30 DS cases (13–18 y ears old) with full trisomy 21 in addition to 30 healthy age-matched controls. The cognitive decline in DS subjects was evaluated using the short form of the Informant Questionnaire on Cognitive Declin...
Source: Indian Journal of Clinical Biochemistry - April 2, 2024 Category: Biochemistry Source Type: research

Establishment of Age Specific Reference Interval for Aminoacids and Acylcarnitine in Dried Blood Spot by Tandem Mass Spectrometry
This study was proposed to establish age specific reference internal for aminoacids and acylcartinitine in dried blood spot by tandem mass spectrometry. A total of 480 apparently healthy children were enrolled for the study and sub classified into four groups as follows: Group A: 0 –1 month, Group B: 1 month–1 year, Group C: 1–5 year and Group D: 5–12 years each having 120 participants. Sample size were calculated as per CLSI approved guidelines. Tables1 and2 presents the age-specific percentile distribution of aminoacids and acylcarnitines established from healthy subjects as per rank-based method recommended...
Source: Indian Journal of Clinical Biochemistry - April 1, 2024 Category: Biochemistry Source Type: research

Crosstalk Between Long Non-coding RNA MALAT1, miRNA-181a, and IL-17 in Cirrhotic Patients and Their Possible Correlation SIRT1/NF- Ƙβ Axis
AbstractIn liver diseases, the interplay of different noncoding RNA expressions, and inflammatory biomarkers show high context dependencies. Interrelations between these noncoding RNA and inflammatory biomarkers paved the way for the diagnosis of various diseases. Here, we analyzed the expression of MALAT1, miR-181a in liver cirrhosis and a panel of pro-inflammatory cytokines (IL-17, SIRT1 and NF- Ƙβ p65). The association between all measured parameters was monitored. Fifty healthy volunteers with normal liver function, hepatic ultrasonography, and negative results for HCV and HBV participated in our study as a healthy c...
Source: Indian Journal of Clinical Biochemistry - March 26, 2024 Category: Biochemistry Source Type: research

Multivariate Statistical Evaluation of 20 Metals/Metalloid Levels in the Serum of Patients with Prostate Gland Diseases
This study elucidates the imbalances of the elements with BPH/PC patients and healthy subjects. For multiple comparisons, Bonferroni test was applied and principal component analysis was performed for measuring the multiple metals/metalloid exposure. Mean concentrations of Al, Cr, Pb, Cd, Na, Ni and K were found higher significantly (p <  0.05) in the serum of BPH patients compared with healthy controls, while average levels of Sb, Al, Cd, As, Mn, Sr, K and Pb were significantly (p <  0.05) elevated in PC patients than controls. The correlation patterns revealed significantly different mutual associations amo...
Source: Indian Journal of Clinical Biochemistry - March 25, 2024 Category: Biochemistry Source Type: research

Global DNA Methylation and Cognitive Impairment: A Population-Based Study from Haryana, North India
This study was conducted among 614 individuals, aged 30 to 79  years from Palwal, Haryana. Cognitive impairment (CI) was assessed using Mini-Mental State Examination (MMSE). Folate, vitamin B12, and homocysteine levels were estimated using chemiluminescence technique. Estimation of global DNA methylation (5mC) levels was performed using the ELISA-based colo rimetric technique. Appropriate comparison tests (based on normality distribution) were applied to compare the levels of global DNA methylation in different study groups. Logistic regression models were run to examine association between global DNA methylation and CI...
Source: Indian Journal of Clinical Biochemistry - March 23, 2024 Category: Biochemistry Source Type: research

Cytotoxic and Apoptotic Effects of Tin(IV) Complexes Containing a Schiff Base Derived from Cephalexin on a Breast Cancer Cell Line
This study involves the synthesis of new metal complexes and their evaluation for cytotoxic and apoptotic effects against human breast cancer cells (MCF-7). The reaction of cephalexin and 4-(dimethylamino)benzaldehyde in boiling methanol gave the corresponding Schiff base in a good yield. Subsequently, the reactions of disubstituted (methyl, butyl, and phenyl) tin chlorides and the Schiff base in boiling methanol produced the corresponding organotin(IV) complexes in high yields. The effect of the synthesized organotin complexes on MCF-7 was assessed. Based on the half-maximal inhibitory concentration (IC50) results, the 3-...
Source: Indian Journal of Clinical Biochemistry - March 23, 2024 Category: Biochemistry Source Type: research