Single-cell profiling transcriptomic reveals cellular heterogeneity and cellular crosstalk in choroidal neovascularization model
Exp Eye Res. 2024 Mar 25:109877. doi: 10.1016/j.exer.2024.109877. Online ahead of print.ABSTRACTChoroidal neovascularization (CNV) is a hallmark of neovascular age-related macular degeneration (nAMD) and a major contributor to vision loss in nAMD cases. However, the identification of specific cell types associated with nAMD remains challenging. Herein, we performed single-cell sequencing to comprehensively explore the cellular diversity and understand the foundational components of the retinal pigment epithelium (RPE)/choroid complex. We unveiled 10 distinct cell types within the RPE/choroid complex. Notably, we observed s...
Source: Experimental Eye Research - March 27, 2024 Category: Opthalmology Authors: Ming Tong Yun Bai Xiaoyan Han Lingjie Kong Ling Ren Linyu Zhang Xiumiao Li Jin Yao Yan Biao Source Type: research

Local administration of myeloid-derived suppressor cells prevents progression of immune-mediated dry eye disease
Exp Eye Res. 2024 Mar 23:109871. doi: 10.1016/j.exer.2024.109871. Online ahead of print.ABSTRACTMyeloid derived suppressor cells (MDSCs) are a heterogenous population of immature hematopoietic precursors with known immunoregulatory functions. The immunosuppressive role of MDSCs has been highlighted in several inflammatory ophthalmic disorders; however, their therapeutic application in suppressing the immune-mediated changes in dry eye disease (DED) have not been studied. We observed significant reduction in antigen presenting cell (APC) frequencies and their maturation in the presence of MDSCs. Moreover, co-culturing MDSCs...
Source: Experimental Eye Research - March 25, 2024 Category: Opthalmology Authors: Pier Luigi Surico Seokjoo Lee Rohan Bir Singh Amirreza Naderi Shilpy Bhullar Tomas Blanco Yihe Chen Reza Dana Source Type: research

Local administration of myeloid-derived suppressor cells prevents progression of immune-mediated dry eye disease
Exp Eye Res. 2024 Mar 23:109871. doi: 10.1016/j.exer.2024.109871. Online ahead of print.ABSTRACTMyeloid derived suppressor cells (MDSCs) are a heterogenous population of immature hematopoietic precursors with known immunoregulatory functions. The immunosuppressive role of MDSCs has been highlighted in several inflammatory ophthalmic disorders; however, their therapeutic application in suppressing the immune-mediated changes in dry eye disease (DED) have not been studied. We observed significant reduction in antigen presenting cell (APC) frequencies and their maturation in the presence of MDSCs. Moreover, co-culturing MDSCs...
Source: Experimental Eye Research - March 25, 2024 Category: Opthalmology Authors: Pier Luigi Surico Seokjoo Lee Rohan Bir Singh Amirreza Naderi Shilpy Bhullar Tomas Blanco Yihe Chen Reza Dana Source Type: research

PEDF-derived peptide protects against Amyloid- β toxicity in vitro and prevents retinal dysfunction in rats
Exp Eye Res. 2024 Mar 22:109861. doi: 10.1016/j.exer.2024.109861. Online ahead of print.ABSTRACTAmyloid-beta (Aβ), a family of aggregation-prone and neurotoxic peptides, has been implicated in the pathophysiology of age-related macular degeneration (AMD). We have previously shown that oligomeric and fibrillar species of Aβ42 exerted retinal toxicity in rats, but while the consequences of exposure to amyloid were related to intracellular effects, the mechanism of Aβ42 internalization in the retina is not well characterized. In the brain, the 67 kDa laminin receptor (67LR) participates in Aβ-related neuronal cell death. ...
Source: Experimental Eye Research - March 24, 2024 Category: Opthalmology Authors: Amanda Qarawani Efrat Naaman Rony Ben- Tzvi Elimelech Michal Harel Chen Itzkovich Shadi Safuri Nitsan Dahan Jack Henkin Shiri Soudry Source Type: research

PEDF-derived peptide protects against Amyloid- β toxicity in vitro and prevents retinal dysfunction in rats
Exp Eye Res. 2024 Mar 22:109861. doi: 10.1016/j.exer.2024.109861. Online ahead of print.ABSTRACTAmyloid-beta (Aβ), a family of aggregation-prone and neurotoxic peptides, has been implicated in the pathophysiology of age-related macular degeneration (AMD). We have previously shown that oligomeric and fibrillar species of Aβ42 exerted retinal toxicity in rats, but while the consequences of exposure to amyloid were related to intracellular effects, the mechanism of Aβ42 internalization in the retina is not well characterized. In the brain, the 67 kDa laminin receptor (67LR) participates in Aβ-related neuronal cell death. ...
Source: Experimental Eye Research - March 24, 2024 Category: Opthalmology Authors: Amanda Qarawani Efrat Naaman Rony Ben- Tzvi Elimelech Michal Harel Chen Itzkovich Shadi Safuri Nitsan Dahan Jack Henkin Shiri Soudry Source Type: research

Role of mRNA-binding proteins in retinal neovascularization
Exp Eye Res. 2024 Mar 19:109870. doi: 10.1016/j.exer.2024.109870. Online ahead of print.ABSTRACTRetinal neovascularization (RNV) is a pathological process that primarily occurs in diabetic retinopathy, retinopathy of prematurity, and retinal vein occlusion. It is a common yet debilitating clinical condition that culminates in blindness. Urgent efforts are required to explore more efficient and less limiting therapeutic strategies. Key RNA-binding proteins (RBPs), crucial for post-transcriptional regulation of gene expression by binding to RNAs, are closely correlated with RNV development. RBP-RNA interactions are altered d...
Source: Experimental Eye Research - March 21, 2024 Category: Opthalmology Authors: Pei Lin Wenye Cao Xuemei Chen Ningzhi Zhang Yiqiao Xing Ning Yang Source Type: research

Photoreceptor deficits appear at eye opening in Rs1 mutant mouse models of X-linked retinoschisis
We report that ERG abnormalities begin near eye-opening and that all ERG components are involved.PMID:38514024 | DOI:10.1016/j.exer.2024.109872 (Source: Experimental Eye Research)
Source: Experimental Eye Research - March 21, 2024 Category: Opthalmology Authors: Matthew J Tarchick Craig Beight Paul B Bonezzi Neal S Peachey Jordan M Renna Source Type: research

Role of mRNA-binding proteins in retinal neovascularization
Exp Eye Res. 2024 Mar 19:109870. doi: 10.1016/j.exer.2024.109870. Online ahead of print.ABSTRACTRetinal neovascularization (RNV) is a pathological process that primarily occurs in diabetic retinopathy, retinopathy of prematurity, and retinal vein occlusion. It is a common yet debilitating clinical condition that culminates in blindness. Urgent efforts are required to explore more efficient and less limiting therapeutic strategies. Key RNA-binding proteins (RBPs), crucial for post-transcriptional regulation of gene expression by binding to RNAs, are closely correlated with RNV development. RBP-RNA interactions are altered d...
Source: Experimental Eye Research - March 21, 2024 Category: Opthalmology Authors: Pei Lin Wenye Cao Xuemei Chen Ningzhi Zhang Yiqiao Xing Ning Yang Source Type: research

Photoreceptor deficits appear at eye opening in Rs1 mutant mouse models of X-linked retinoschisis
We report that ERG abnormalities begin near eye-opening and that all ERG components are involved.PMID:38514024 | DOI:10.1016/j.exer.2024.109872 (Source: Experimental Eye Research)
Source: Experimental Eye Research - March 21, 2024 Category: Opthalmology Authors: Matthew J Tarchick Craig Beight Paul B Bonezzi Neal S Peachey Jordan M Renna Source Type: research

Role of mRNA-binding proteins in retinal neovascularization
Exp Eye Res. 2024 Mar 19:109870. doi: 10.1016/j.exer.2024.109870. Online ahead of print.ABSTRACTRetinal neovascularization (RNV) is a pathological process that primarily occurs in diabetic retinopathy, retinopathy of prematurity, and retinal vein occlusion. It is a common yet debilitating clinical condition that culminates in blindness. Urgent efforts are required to explore more efficient and less limiting therapeutic strategies. Key RNA-binding proteins (RBPs), crucial for post-transcriptional regulation of gene expression by binding to RNAs, are closely correlated with RNV development. RBP-RNA interactions are altered d...
Source: Experimental Eye Research - March 21, 2024 Category: Opthalmology Authors: Pei Lin Wenye Cao Xuemei Chen Ningzhi Zhang Yiqiao Xing Ning Yang Source Type: research

Photoreceptor deficits appear at eye opening in Rs1 mutant mouse models of X-linked retinoschisis
We report that ERG abnormalities begin near eye-opening and that all ERG components are involved.PMID:38514024 | DOI:10.1016/j.exer.2024.109872 (Source: Experimental Eye Research)
Source: Experimental Eye Research - March 21, 2024 Category: Opthalmology Authors: Matthew J Tarchick Craig Beight Paul B Bonezzi Neal S Peachey Jordan M Renna Source Type: research

Mesenchymal stem cell-based adjunctive therapy for Pseudomonas aeruginosa-induced keratitis: A proof-of-concept in-vitro
CONCLUSION: MSC-CM holds promise as an adjunctive therapeutic approach for P. aeruginosa-induced corneal epithelial damage.PMID:38494102 | DOI:10.1016/j.exer.2024.109863 (Source: Experimental Eye Research)
Source: Experimental Eye Research - March 17, 2024 Category: Opthalmology Authors: Sreya Mitra Vasudeva Tati Prabhudatta Das Joveeta Joseph Bhupesh Bagga Sachin Shukla Source Type: research

Mesenchymal stem cell-based adjunctive therapy for Pseudomonas aeruginosa-induced keratitis: A proof-of-concept in-vitro
CONCLUSION: MSC-CM holds promise as an adjunctive therapeutic approach for P. aeruginosa-induced corneal epithelial damage.PMID:38494102 | DOI:10.1016/j.exer.2024.109863 (Source: Experimental Eye Research)
Source: Experimental Eye Research - March 17, 2024 Category: Opthalmology Authors: Sreya Mitra Vasudeva Tati Prabhudatta Das Joveeta Joseph Bhupesh Bagga Sachin Shukla Source Type: research

Mesenchymal stem cell-based adjunctive therapy for Pseudomonas aeruginosa-induced keratitis: A proof-of-concept in-vitro
CONCLUSION: MSC-CM holds promise as an adjunctive therapeutic approach for P. aeruginosa-induced corneal epithelial damage.PMID:38494102 | DOI:10.1016/j.exer.2024.109863 (Source: Experimental Eye Research)
Source: Experimental Eye Research - March 17, 2024 Category: Opthalmology Authors: Sreya Mitra Vasudeva Tati Prabhudatta Das Joveeta Joseph Bhupesh Bagga Sachin Shukla Source Type: research

Mesenchymal stem cell-based adjunctive therapy for Pseudomonas aeruginosa-induced keratitis: A proof-of-concept in-vitro
CONCLUSION: MSC-CM holds promise as an adjunctive therapeutic approach for P. aeruginosa-induced corneal epithelial damage.PMID:38494102 | DOI:10.1016/j.exer.2024.109863 (Source: Experimental Eye Research)
Source: Experimental Eye Research - March 17, 2024 Category: Opthalmology Authors: Sreya Mitra Vasudeva Tati Prabhudatta Das Joveeta Joseph Bhupesh Bagga Sachin Shukla Source Type: research