Septin-dependent defense mechanisms against Pseudomonas aeruginosa are stalled in cystic fibrosis bronchial epithelial cells
This study aimed to investigate the role of SEPT7 in controlling P. aeruginosa infection in bronchial epithelial cells, particularly in CF. The study findings showed that SEPT7 encages P. aeruginosa in bronchial epithelial cells and its inhibition downregulates the expression of other SEPTs. In addition, P. aeruginosa does not regulate SEPT7 expression. Finally, we found that inhibiting SEPT7 expression in bronchial epithelial cells (BEAS-2B 16HBE14o- and primary cells) resulted in higher levels of internalized P. aeruginosa and decreased IL-6 production during infection, suggesting a crucial role of SEPT7 in the host resp...
Source: European Journal of Cell Biology - April 18, 2024 Category: Cytology Authors: Sylvain Brax Cl émence Gaudin Claire Calmel Pierre-Yves Bo ëlle Harriet Corvol Manon Ruffin Lo ïc Guillot Source Type: research
Transmembrane signaling through single-spanning receptors modulated by phase separation at the cell surface
This study describes the emerging features of transmembrane signaling through single-spanning receptors from the perspective of phase separation. Possible physicochemical modulations of LLPS-based transmembrane signaling are also discussed.PMID:38631097 | DOI:10.1016/j.ejcb.2024.151413 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - April 17, 2024 Category: Cytology Authors: Toshiyuki Murai Source Type: research
Role of the 3-mercaptopyruvate sulfurtransferase in colon/colorectal cancers
Eur J Cell Biol. 2024 Apr 15;103(2):151415. doi: 10.1016/j.ejcb.2024.151415. Online ahead of print.ABSTRACTThe 3-mercaptopyruvate sulfurtransferase (MPST) is a protein persulfidase, occurring mainly in mitochondria. Although function of this protein in cancer cells has been already studied, no clear outcome can be postulated up to now. Therefore, we focused on the determination of function of MPST in colon (HCT116 cells)/colorectal (DLD1 cells) cancers. In silico analysis revealed that in gastrointestinal cancers, MPST together with its binding partners can be either of a high risk or might have a protective effect. Silenc...
Source: European Journal of Cell Biology - April 17, 2024 Category: Cytology Authors: Katarina Matyasova Andrea Soltysova Petr Babula Olga Krizanova Veronika Liskova Source Type: research
Cause and chondroprotective effects of prostaglandin E2 secretion during mesenchymal stromal cell chondrogenesis
This study aimed to identify drivers of PGE2 and its role in the inadvertent MSC misdifferentiation into hypertrophic chondrocytes. PGE2 release, which rose in the first three weeks of MSC chondrogenesis, was jointly stimulated by endogenous BMP, WNT, and hedgehog activity that supported the exogenous stimulation by TGF-β1 and insulin to overcome the PGE2 inhibition by dexamethasone. Experiments with PGE2 treatment or the inhibitor celecoxib or specific receptor antagonists demonstrated that PGE2, although driven by prohypertrophic signals, exerted broad autocrine antihypertrophic effects. This chondroprotective effect ma...
Source: European Journal of Cell Biology - April 12, 2024 Category: Cytology Authors: Sven Schmidt Felicia A M Klampfleuthner Tobias Renkawitz Solvig Diederichs Source Type: research
Cause and chondroprotective effects of prostaglandin E2 secretion during mesenchymal stromal cell chondrogenesis
This study aimed to identify drivers of PGE2 and its role in the inadvertent MSC misdifferentiation into hypertrophic chondrocytes. PGE2 release, which rose in the first three weeks of MSC chondrogenesis, was jointly stimulated by endogenous BMP, WNT, and hedgehog activity that supported the exogenous stimulation by TGF-β1 and insulin to overcome the PGE2 inhibition by dexamethasone. Experiments with PGE2 treatment or the inhibitor celecoxib or specific receptor antagonists demonstrated that PGE2, although driven by prohypertrophic signals, exerted broad autocrine antihypertrophic effects. This chondroprotective effect ma...
Source: European Journal of Cell Biology - April 12, 2024 Category: Cytology Authors: Sven Schmidt Felicia A M Klampfleuthner Tobias Renkawitz Solvig Diederichs Source Type: research
Cause and chondroprotective effects of prostaglandin E2 secretion during mesenchymal stromal cell chondrogenesis
This study aimed to identify drivers of PGE2 and its role in the inadvertent MSC misdifferentiation into hypertrophic chondrocytes. PGE2 release, which rose in the first three weeks of MSC chondrogenesis, was jointly stimulated by endogenous BMP, WNT, and hedgehog activity that supported the exogenous stimulation by TGF-β1 and insulin to overcome the PGE2 inhibition by dexamethasone. Experiments with PGE2 treatment or the inhibitor celecoxib or specific receptor antagonists demonstrated that PGE2, although driven by prohypertrophic signals, exerted broad autocrine antihypertrophic effects. This chondroprotective effect ma...
Source: European Journal of Cell Biology - April 12, 2024 Category: Cytology Authors: Sven Schmidt Felicia A M Klampfleuthner Tobias Renkawitz Solvig Diederichs Source Type: research
Cause and chondroprotective effects of prostaglandin E2 secretion during mesenchymal stromal cell chondrogenesis
This study aimed to identify drivers of PGE2 and its role in the inadvertent MSC misdifferentiation into hypertrophic chondrocytes. PGE2 release, which rose in the first three weeks of MSC chondrogenesis, was jointly stimulated by endogenous BMP, WNT, and hedgehog activity that supported the exogenous stimulation by TGF-β1 and insulin to overcome the PGE2 inhibition by dexamethasone. Experiments with PGE2 treatment or the inhibitor celecoxib or specific receptor antagonists demonstrated that PGE2, although driven by prohypertrophic signals, exerted broad autocrine antihypertrophic effects. This chondroprotective effect ma...
Source: European Journal of Cell Biology - April 12, 2024 Category: Cytology Authors: Sven Schmidt Felicia A M Klampfleuthner Tobias Renkawitz Solvig Diederichs Source Type: research
Olfactory receptors impact pathophysiological processes of lung diseases in bronchial epithelial cells
CONCLUSION: ORs might be promising drug target candidates for lung diseases with non-type 2 inflammation. Their stimulation might reduce inflammation or prevent tissue remodeling by promoting wound healing.PMID:38583306 | DOI:10.1016/j.ejcb.2024.151408 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - April 7, 2024 Category: Cytology Authors: Daniel Weidinger Julian Jacobsen Desiree Alisch Hendrik Uebner Natalie Heinen Lea Greune Saskia Westhoven Kaschin Jamal Jameel Juliane Kronsbein Stephanie Pfaender Christian Taube Sebastian Reuter Marcus Peters Hanns Hatt J ürgen Knobloch Source Type: research
Olfactory receptors impact pathophysiological processes of lung diseases in bronchial epithelial cells
CONCLUSION: ORs might be promising drug target candidates for lung diseases with non-type 2 inflammation. Their stimulation might reduce inflammation or prevent tissue remodeling by promoting wound healing.PMID:38583306 | DOI:10.1016/j.ejcb.2024.151408 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - April 7, 2024 Category: Cytology Authors: Daniel Weidinger Julian Jacobsen Desiree Alisch Hendrik Uebner Natalie Heinen Lea Greune Saskia Westhoven Kaschin Jamal Jameel Juliane Kronsbein Stephanie Pfaender Christian Taube Sebastian Reuter Marcus Peters Hanns Hatt J ürgen Knobloch Source Type: research
Olfactory receptors impact pathophysiological processes of lung diseases in bronchial epithelial cells
CONCLUSION: ORs might be promising drug target candidates for lung diseases with non-type 2 inflammation. Their stimulation might reduce inflammation or prevent tissue remodeling by promoting wound healing.PMID:38583306 | DOI:10.1016/j.ejcb.2024.151408 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - April 7, 2024 Category: Cytology Authors: Daniel Weidinger Julian Jacobsen Desiree Alisch Hendrik Uebner Natalie Heinen Lea Greune Saskia Westhoven Kaschin Jamal Jameel Juliane Kronsbein Stephanie Pfaender Christian Taube Sebastian Reuter Marcus Peters Hanns Hatt J ürgen Knobloch Source Type: research
Olfactory receptors impact pathophysiological processes of lung diseases in bronchial epithelial cells
CONCLUSION: ORs might be promising drug target candidates for lung diseases with non-type 2 inflammation. Their stimulation might reduce inflammation or prevent tissue remodeling by promoting wound healing.PMID:38583306 | DOI:10.1016/j.ejcb.2024.151408 (Source: European Journal of Cell Biology)
Source: European Journal of Cell Biology - April 7, 2024 Category: Cytology Authors: Daniel Weidinger Julian Jacobsen Desiree Alisch Hendrik Uebner Natalie Heinen Lea Greune Saskia Westhoven Kaschin Jamal Jameel Juliane Kronsbein Stephanie Pfaender Christian Taube Sebastian Reuter Marcus Peters Hanns Hatt J ürgen Knobloch Source Type: research
Degradation of hexosylceramides is required for timely corpse clearance via formation of cargo-containing phagolysosomal vesicles
Eur J Cell Biol. 2024 Apr 5;103(2):151411. doi: 10.1016/j.ejcb.2024.151411. Online ahead of print.ABSTRACTEfficient degradation of phagocytic cargo in lysosomes is crucial to maintain cellular homeostasis and defending cells against pathogens. However, the mechanisms underlying the degradation and recycling of macromolecular cargo within the phagolysosome remain incompletely understood. We previously reported that the phagolysosome containing the corpse of the polar body in C. elegans tubulates into small vesicles to facilitate corpse clearance, a process that requires cargo protein degradation and amino acid export. Here ...
Source: European Journal of Cell Biology - April 6, 2024 Category: Cytology Authors: Rebecca Holzapfel Agata Prell Fabian Schumacher Veronika Perschin Jos é Pedro Friedmann Angeli Burkhard Kleuser Christian Stigloher Gholamreza Fazeli Source Type: research
Degradation of hexosylceramides is required for timely corpse clearance via formation of cargo-containing phagolysosomal vesicles
Eur J Cell Biol. 2024 Apr 5;103(2):151411. doi: 10.1016/j.ejcb.2024.151411. Online ahead of print.ABSTRACTEfficient degradation of phagocytic cargo in lysosomes is crucial to maintain cellular homeostasis and defending cells against pathogens. However, the mechanisms underlying the degradation and recycling of macromolecular cargo within the phagolysosome remain incompletely understood. We previously reported that the phagolysosome containing the corpse of the polar body in C. elegans tubulates into small vesicles to facilitate corpse clearance, a process that requires cargo protein degradation and amino acid export. Here ...
Source: European Journal of Cell Biology - April 6, 2024 Category: Cytology Authors: Rebecca Holzapfel Agata Prell Fabian Schumacher Veronika Perschin Jos é Pedro Friedmann Angeli Burkhard Kleuser Christian Stigloher Gholamreza Fazeli Source Type: research
Damage control of epithelial barrier function in dynamic environments
Eur J Cell Biol. 2024 Apr 3;103(2):151410. doi: 10.1016/j.ejcb.2024.151410. Online ahead of print.ABSTRACTEpithelial tissues cover the surfaces and lumens of the internal organs of multicellular animals and crucially contribute to internal environment homeostasis by delineating distinct compartments within the body. This vital role is known as epithelial barrier function. Epithelial cells are arranged like cobblestones and intricately bind together to form an epithelial sheet that upholds this barrier function. Central to the restriction of solute and fluid diffusion through intercellular spaces are occluding junctions, ti...
Source: European Journal of Cell Biology - April 5, 2024 Category: Cytology Authors: Tomohito Higashi Akira C Saito Hideki Chiba Source Type: research
Cortactin interacts with αDystrobrevin-1 and regulates murine neuromuscular junction morphology
Eur J Cell Biol. 2024 Mar 31;103(2):151409. doi: 10.1016/j.ejcb.2024.151409. Online ahead of print.ABSTRACTNeuromuscular junctions transmit signals from the nervous system to skeletal muscles, triggering their contraction, and their proper organization is essential for breathing and voluntary movements. αDystrobrevin-1 is a cytoplasmic component of the dystrophin-glycoprotein complex and has pivotal functions in regulating the integrity of muscle fibers and neuromuscular junctions. Previous studies identified that αDystrobrevin-1 functions in the organization of the neuromuscular junction and that its phosphorylation in ...
Source: European Journal of Cell Biology - April 5, 2024 Category: Cytology Authors: Teresa De Cicco Marcin P ęziński Olga W ójcicka Bhola Shankar Pradhan Margareta Jab łońska Klemens Rottner Tomasz J Pr ószyński Source Type: research
