Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Short communication: The boring sponge (Pione vastifica, Hancock, 1849) induces oxidative stress in the Pacific oyster (Magallana gigas, Thunberg, 1793)
In this study, we investigated the effects of boring sponge on the activity of three antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GP)) in the mantle, and the production of reactive oxygen species (ROS) and potential genotoxicity in hemocytes of the Pacific oyster Magallana gigas. Our results showed a significant increase in ROS production and DNA damage in hemocytes. Notably, the activity of SOD, CAT, and GP in the mantle was not significantly affected by boring sponge infection. Collectively, these results suggest that sponge invasion may cause oxidative stress in Pacific oys...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 18, 2024 Category: Biochemistry Authors: Elina S Chelebieva Daria S Lavrichenko Olga L Gostyukhina Maria S Podolskaya Ekaterina S Kladchenko Source Type: research
Establishment and characterization of a kidney cell line from hybrid snakehead (male Channa argus × female Channa maculata) and its susceptibility to hybrid snakehead rhabdovirus (HSHRV)
In this study, a fish cell line called CAMK, derived from the kidneys of hybrid snakehead, was established and characterized. CAMK cells exhibited the maximum growth rate at 28 °C in Leibovitz's-15 medium supplemented with 10% fetal bovine serum(FBS). Karyotyping revealed diploid chromosomes in 54% of the cells at the 50th passage (2n = 66), and 16S rRNA sequencing validated that CAMK cells originated fromhybrid snakehead, and the detection of kidney-specific antibodies suggested that it originated from kidney. .The culture was free from mycoplasma contamination, and the green fluorescent protein gene was effectively tran...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 15, 2024 Category: Biochemistry Authors: Huiling Deng Shucheng Zheng Yingying Li Xubing Mo Jian Zhao Jiyuan Yin Cunbin Shi Qing Wang Yingying Wang Source Type: research
Establishment and characterization of a kidney cell line from hybrid snakehead (male Channa argus × female Channa maculata) and its susceptibility to hybrid snakehead rhabdovirus (HSHRV)
In this study, a fish cell line called CAMK, derived from the kidneys of hybrid snakehead, was established and characterized. CAMK cells exhibited the maximum growth rate at 28 °C in Leibovitz's-15 medium supplemented with 10% fetal bovine serum(FBS). Karyotyping revealed diploid chromosomes in 54% of the cells at the 50th passage (2n = 66), and 16S rRNA sequencing validated that CAMK cells originated fromhybrid snakehead, and the detection of kidney-specific antibodies suggested that it originated from kidney. .The culture was free from mycoplasma contamination, and the green fluorescent protein gene was effectively tran...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 15, 2024 Category: Biochemistry Authors: Huiling Deng Shucheng Zheng Yingying Li Xubing Mo Jian Zhao Jiyuan Yin Cunbin Shi Qing Wang Yingying Wang Source Type: research
Establishment and characterization of a kidney cell line from hybrid snakehead (male Channa argus × female Channa maculata) and its susceptibility to hybrid snakehead rhabdovirus (HSHRV)
In this study, a fish cell line called CAMK, derived from the kidneys of hybrid snakehead, was established and characterized. CAMK cells exhibited the maximum growth rate at 28 °C in Leibovitz's-15 medium supplemented with 10% fetal bovine serum(FBS). Karyotyping revealed diploid chromosomes in 54% of the cells at the 50th passage (2n = 66), and 16S rRNA sequencing validated that CAMK cells originated fromhybrid snakehead, and the detection of kidney-specific antibodies suggested that it originated from kidney. .The culture was free from mycoplasma contamination, and the green fluorescent protein gene was effectively tran...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 15, 2024 Category: Biochemistry Authors: Huiling Deng Shucheng Zheng Yingying Li Xubing Mo Jian Zhao Jiyuan Yin Cunbin Shi Qing Wang Yingying Wang Source Type: research
Growth performance, health status, gut microbiome, and expression of immune and growth-related genes of rainbow trout (Oncorhynchus mykiss) fed diets with pea protein replacement of fish meal
Comp Biochem Physiol B Biochem Mol Biol. 2024 Apr 9;273:110968. doi: 10.1016/j.cbpb.2024.110968. Online ahead of print.ABSTRACTThe need for fish meal constrains fish farming and significantly impacts sustainability of the aquaculture industry. Hence, it is important to investigate the use of plant-based protein sources in fish diets. The present study was conducted to determine the effects of different levels of fish meal (FM) replacement by pea protein (PP) in a 60-day feeding experiment in rainbow trout, Oncorhynchus mykiss. Effects on growth performance, body composition, hematology, serum biochemistry and immunology, a...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 11, 2024 Category: Biochemistry Authors: Osman Sabri Kesbi ç Ümit Acar Fevziye I şıl Kesbiç Sevdan Y ılmaz Source Type: research
Maximum storage time of refrigerated blood and frozen plasma samples from tambaqui, Colossoma macropomum
This study sought to determine the maximum time refrigerated blood and frozen plasma samples of the tambaqui, Colossoma macropomum, can be stored without affecting analysis. Samples from 12 fish were obtained, stored under refrigeration at 4 °C and evaluated after 0, 24, 48, 72, and 96 h, while samples from 14 fish were centrifuged, and the resulting plasma was frozen at -20 °C and then evaluated after 0, 8, 12, 16 and 20 weeks. The parameters analyzed were hematocrit (Ht), hemoglobin content (Hb), total erythrocytes (RBC), total (WBC) and differential leukocytes, total thrombocytes (TC), glucose content (Glc), total pro...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 11, 2024 Category: Biochemistry Authors: Marcela C Sena F ábio C Sterzelecki Paola Fabiana F Gomes Helen Cristiane A Souza Nuno Filipe A Correia de Melo J Natividade B Baldisserotto Glauber David A Palheta Simone do Socorro D Santos Source Type: research
Molecular characterization of transcription factor CREB3L2 and CREB3L3 and their role in melanogenesis in Pacific oysters (Crassostrea gigas)
In this study, we characterized two CREB genes (CREB3L2 and CREB3L3) from Pacific oysters. Both of them contained a conserved DNA-binding and dimerization domain (a basic-leucine zipper domain). CREB3L2 and CREB3L3 were expressed highly in the mantle tissues and exhibited higher expression levels in the black-shell oyster than in the white. Masson-Fontana melanin staining and immunofluorescence analysis showed that the location of CREB3L2 protein was generally consistent with the distribution of melanin in oyster edge mantle. Dual-luciferase reporter assays revealed that CREB3L2 and CREB3L3 could activate the microphthalmi...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 11, 2024 Category: Biochemistry Authors: Kunyin Jiang Hong Yu Lingfeng Kong Shikai Liu Qi Li Source Type: research
Growth performance, health status, gut microbiome, and expression of immune and growth-related genes of rainbow trout (Oncorhynchus mykiss) fed diets with pea protein replacement of fish meal
Comp Biochem Physiol B Biochem Mol Biol. 2024 Apr 9:110968. doi: 10.1016/j.cbpb.2024.110968. Online ahead of print.ABSTRACTThe need for fish meal constrains fish farming and significantly impacts sustainability of the aquaculture industry. Hence, it is important to investigate the use of plant-based protein sources in fish diets. The present study was conducted to determine the effects of different levels of fish meal (FM) replacement by pea protein (PP) in a 60-day feeding experiment in rainbow trout, Oncorhynchus mykiss. Effects on growth performance, body composition, hematology, serum biochemistry and immunology, and i...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 11, 2024 Category: Biochemistry Authors: Osman Sabri Kesbi ç Ümit Acar Fevziye I şıl Kesbiç Sevdan Y ılmaz Source Type: research
Maximum storage time of refrigerated blood and frozen plasma samples from tambaqui, Colossoma macropomum
This study sought to determine the maximum time refrigerated blood and frozen plasma samples of the tambaqui, Colossoma macropomum, can be stored without affecting analysis. Samples from 12 fish were obtained, stored under refrigeration at 4 °C and evaluated after 0, 24, 48, 72, and 96 h, while samples from 14 fish were centrifuged, and the resulting plasma was frozen at -20 °C and then evaluated after 0, 8, 12, 16 and 20 weeks. The parameters analyzed were hematocrit (Ht), hemoglobin content (Hb), total erythrocytes (RBC), total (WBC) and differential leukocytes, total thrombocytes (TC), glucose content (Glc), total pro...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 11, 2024 Category: Biochemistry Authors: Marcela C Sena F ábio C Sterzelecki Paola Fabiana F Gomes Helen Cristiane A Souza Nuno Filipe A Correia de Melo J Natividade B Baldisserotto Glauber David A Palheta Simone do Socorro D Santos Source Type: research
Molecular characterization of transcription factor CREB3L2 and CREB3L3 and their role in melanogenesis in Pacific oysters (Crassostrea gigas)
In this study, we characterized two CREB genes (CREB3L2 and CREB3L3) from Pacific oysters. Both of them contained a conserved DNA-binding and dimerization domain (a basic-leucine zipper domain). CREB3L2 and CREB3L3 were expressed highly in the mantle tissues and exhibited higher expression levels in the black-shell oyster than in the white. Masson-Fontana melanin staining and immunofluorescence analysis showed that the location of CREB3L2 protein was generally consistent with the distribution of melanin in oyster edge mantle. Dual-luciferase reporter assays revealed that CREB3L2 and CREB3L3 could activate the microphthalmi...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 11, 2024 Category: Biochemistry Authors: Kunyin Jiang Hong Yu Lingfeng Kong Shikai Liu Qi Li Source Type: research
Growth performance, health status, gut microbiome, and expression of immune and growth-related genes of rainbow trout (Oncorhynchus mykiss) fed diets with pea protein replacement of fish meal
Comp Biochem Physiol B Biochem Mol Biol. 2024 Apr 9:110968. doi: 10.1016/j.cbpb.2024.110968. Online ahead of print.ABSTRACTThe need for fish meal constrains fish farming and significantly impacts sustainability of the aquaculture industry. Hence, it is important to investigate the use of plant-based protein sources in fish diets. The present study was conducted to determine the effects of different levels of fish meal (FM) replacement by pea protein (PP) in a 60-day feeding experiment in rainbow trout, Oncorhynchus mykiss. Effects on growth performance, body composition, hematology, serum biochemistry and immunology, and i...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 11, 2024 Category: Biochemistry Authors: Osman Sabri Kesbi ç Ümit Acar Fevziye I şıl Kesbiç Sevdan Y ılmaz Source Type: research
Maximum storage time of refrigerated blood and frozen plasma samples from tambaqui, Colossoma macropomum
This study sought to determine the maximum time refrigerated blood and frozen plasma samples of the tambaqui, Colossoma macropomum, can be stored without affecting analysis. Samples from 12 fish were obtained, stored under refrigeration at 4 °C and evaluated after 0, 24, 48, 72, and 96 h, while samples from 14 fish were centrifuged, and the resulting plasma was frozen at -20 °C and then evaluated after 0, 8, 12, 16 and 20 weeks. The parameters analyzed were hematocrit (Ht), hemoglobin content (Hb), total erythrocytes (RBC), total (WBC) and differential leukocytes, total thrombocytes (TC), glucose content (Glc), total pro...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 11, 2024 Category: Biochemistry Authors: Marcela C Sena F ábio C Sterzelecki Paola Fabiana F Gomes Helen Cristiane A Souza Nuno Filipe A Correia de Melo J Natividade B Baldisserotto Glauber David A Palheta Simone do Socorro D Santos Source Type: research
Molecular characterization of transcription factor CREB3L2 and CREB3L3 and their role in melanogenesis in Pacific oysters (Crassostrea gigas)
In this study, we characterized two CREB genes (CREB3L2 and CREB3L3) from Pacific oysters. Both of them contained a conserved DNA-binding and dimerization domain (a basic-leucine zipper domain). CREB3L2 and CREB3L3 were expressed highly in the mantle tissues and exhibited higher expression levels in the black-shell oyster than in the white. Masson-Fontana melanin staining and immunofluorescence analysis showed that the location of CREB3L2 protein was generally consistent with the distribution of melanin in oyster edge mantle. Dual-luciferase reporter assays revealed that CREB3L2 and CREB3L3 could activate the microphthalmi...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 11, 2024 Category: Biochemistry Authors: Kunyin Jiang Hong Yu Lingfeng Kong Shikai Liu Qi Li Source Type: research
MicroRNA miR-7-5p targets MARK2 to control metamorphosis in Galeruca daurica
This study reveals that the accurate regulation of miRNAs and their target genes is indispensable for insect metamorphosis.PMID:38521445 | DOI:10.1016/j.cbpb.2024.110967 (Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology.)
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - March 23, 2024 Category: Biochemistry Authors: Hai-Chao Wang Ling Li Jing-Hang Zhang Zhi-Han Yao Bao-Ping Pang Source Type: research
MicroRNA miR-7-5p targets MARK2 to control metamorphosis in Galeruca daurica
This study reveals that the accurate regulation of miRNAs and their target genes is indispensable for insect metamorphosis.PMID:38521445 | DOI:10.1016/j.cbpb.2024.110967 (Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology.)
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - March 23, 2024 Category: Biochemistry Authors: Hai-Chao Wang Ling Li Jing-Hang Zhang Zhi-Han Yao Bao-Ping Pang Source Type: research
