Biotechnology and Applied Biochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

In vitro antibacterial activity of antibiotics and plant essential oils against Escherichia coli MTCC443 supported through the molecular docking and pharmacokinetics study
This study has been made to compare the in vitro antibacterial activity and further validated the same through the molecular docking study of 13 antibiotics such as ciprofloxacin, chloramphenicol, erythromycin, ampicillin, cefotaxime, rifampicin, kanamycin, vancomycin, streptomycin, penicillin, nalidixic acid, trimethoprim, and polymyxin, and 10 EOs such as garlic, tulsi, neem, clove, thyme, peppermint, coriander, tea, lavender, and eucalyptus against the target protein (DNA gyrase) ofE. coli MTCC443.E. coli Microbial Type Culture Collection 443 was found to be highly sensitive to ciprofloxacin (zone of inhibition [ZOI], 2...
Source: Biotechnology and Applied Biochemistry - April 17, 2024 Category: Biochemistry Authors: Saurabh Jain, Adarsh Kumar Shukla, Surbhi Panwar, Rajesh Kumar, Ashwani Kumar Tags: ORIGINAL ARTICLE Source Type: research

Characterization and validation of fatty acid metabolism ‐related genes predicting prognosis, immune infiltration, and drug sensitivity in endometrial cancer
AbstractEndometrial cancer is considered to be the second most common tumor of the female reproductive system, and patients diagnosed with advanced endometrial cancer have a poor prognosis. The influence of fatty acid metabolism in the prognosis of patients with endometrial cancer remains unclear. We constructed a prognostic risk model using transcriptome sequencing data of endometrial cancer and clinical information of patients from The Cancer Genome Atlas (TCGA) database via least absolute shrinkage and selection operator regression analysis. The tumor immune microenvironment was analyzed using the CIBERSORT algorithm, f...
Source: Biotechnology and Applied Biochemistry - April 15, 2024 Category: Biochemistry Authors: Haojia Li, Ting Zhou, Qi Zhang, Yuwei Yao, Teng Hua, Jun Zhang, Hongbo Wang Tags: ORIGINAL ARTICLE Source Type: research

Sensitive osteoarthritis sensing by salt ‐induced aggregation and dispersion of gold nanoparticles
AbstractOsteoarthritis occurs in any joints, and identification in its earlier stages helps to treat the disease and increase the recovery rate. The radiography method and imaging techniques are traditionally used to identify osteoarthritis. But these methods are expensive, and with the complicated steps. Researchers are working toward developing a highly sensitive biosensor in identifying the osteoarthritis biomarker. This research was focused on developing a C-terminal telopeptide of type II collagen (CTX-II) colorimetric sensor with gold nanoparticle (AuNP) for diagnosing osteoarthritis. Anti-CTX-II was conjugated with ...
Source: Biotechnology and Applied Biochemistry - April 13, 2024 Category: Biochemistry Authors: Yong Fan, Renjie Li, Xuekai Deng, Ming Li, Yu Zhou, Dan Wu, Gang Liu Tags: ORIGINAL ARTICLE Source Type: research

The effects of hyaluronan and proteoglycan link protein 1 (HAPLN1) in ameliorating spinal cord injury mediated by Nrf2
In this study, both in vitro and in vivo SCI models were established. Nissl staining and terminal deoxynucleotidyl transferase dUTP nick end labeling staining assays were used. Western blotting and enzyme-linked immunosorbent assay were employed to assess the expression of proteins. Our results demonstrate that the administration of HAPLN1 promoted the recovery of motor neurons after SCI by increasing the Basso mouse scale score, increasing the numbers of motor neurons, and preventing apoptosis of spinal cord cells. Additionally, HAPLN1 mitigated OS in spinal cord tissue after SCI by increasing the content of superoxide di...
Source: Biotechnology and Applied Biochemistry - April 12, 2024 Category: Biochemistry Authors: Hongzhi Yang, Bin Hu, Xichun Wang, Wenjie Chen, Huanbin Zhou Tags: ORIGINAL ARTICLE Source Type: research

Issue Information
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Source: Biotechnology and Applied Biochemistry - April 10, 2024 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research

Facile construction of gefitinib ‐loaded zeolitic imidazolate framework nanocomposites for the treatment of different lung cancer cells
AbstractGefitinib (GET) is a revolutionary targeted treatment inhibiting the epidermal growth factor receptor's tyrosine kinase action by competitively inhibiting the ATP binding site. In preclinical trials, several lung cancer cell lines and xenografts have demonstrated potential activity with GET. Response rates neared 25% in preclinical trials for non-small cell lung cancer. Here, we describe the one-pot synthesis of GET@ZIF-8 nanocomposites (NCs) in pure water, encapsulating zeolitic imidazolate framework 8 (ZIF-8). This method developed NCs with consistent morphology and a loading efficiency of 9%, resulting in a load...
Source: Biotechnology and Applied Biochemistry - April 10, 2024 Category: Biochemistry Authors: Kalaivani Aiyasamy, Malathi Ramasamy, Abdurahman Hajinur Hirad, Palanisamy Arulselvan, Ravindran Jaganathan, Jagadeesh Suriyaprakash, Indumathi Thangavelu, Abdullah A. Alarfaj Tags: ORIGINAL ARTICLE Source Type: research

Overview of cloning in lactic acid bacteria: Expression and its application of probiotic potential in inflammatory bowel diseases
AbstractInflammatory bowel disease (IBD) imposes a significant impact on the quality of life for affected individuals. However, there was a current lack of a systematic summary regarding the latest epidemic trends and the underlying pathogenesis of IBD. This highlights the need for a thorough examination of both the epidemiological aspects of IBD and the specific mechanisms by which lactic acid bacteria (LAB) contribute to mitigating this condition. In developed countries, higher incidences and death rates of IBD have been observed, influenced by a combination of environmental and genetic factors. LAB offer significant adv...
Source: Biotechnology and Applied Biochemistry - April 5, 2024 Category: Biochemistry Authors: K. E. Vivekanandan, R. Kasimani, P. Vinoth Kumar, S. Meenatchisundaram, William Arputha Prabhu Tags: REVIEW ARTICLE Source Type: research

CB ‐0821, a novel CC chemokine receptor 5 (CCR5) inhibitor with improved binding efficacy proposed as anti‐HIV candidate: Computational and in vitro approach
In this study, CB-0821, identified from the ChemBridge library, emerged as a promising CCR5 inhibitor. Molecular dynamics simulations indicate comparable dynamic properties for CB-0821 and MVC. In silico comparisons with other CCR5 inhibitors emphasize CB-0821's superior binding affinity, positioning it as a potential lead compound. Evaluations of the dissociation constant (Ki) and absorption, distribution, metabolism, and excretion predictions suggest CB-0821 as a well-tolerated drug. Furthermore, the dose-dependent inhibition of CCR5 by CB-0821 in Peripheral blood mononuclear cells (PBMCs) (ranging from 10 to 200  nM) d...
Source: Biotechnology and Applied Biochemistry - April 3, 2024 Category: Biochemistry Authors: Ashish Kumar Tags: ORIGINAL ARTICLE Source Type: research

Microsatellite instability evaluation by a novel PCR ‐based 8‐loci test kit in colorectal cancer
This study evaluated the reliability and accuracy of a novel polymerase chain reaction (PCR)-based 8-loci MSI test kit, a rapid test kit designed to detect MSI, by comparing its performance with immunohistochemistry (IHC) and the National Cancer Institute (NCI) 2B3D Panel. MSI status was determined in 186 formalin-fixed paraffin-embedded (FFPE) colorectal cancer tissue samples with known mismatch repair (MMR) status by IHC using the novel PCR-based 8-loci MSI test kit. Additionally, the consistency between the NCI 2B3D Panel and the novel PCR-based 8-loci panel was compared using 69 FFPE tumor tissues paired with adjacent ...
Source: Biotechnology and Applied Biochemistry - April 1, 2024 Category: Biochemistry Authors: Gaofang Xiao, Jing Li, Lijun Deng, Shuangquan Gao, Caiyun Tan, Guiqing He, Richang Du Tags: ORIGINAL ARTICLE Source Type: research

The effect of thyme essential oil and endothelial progenitor stem cells on lipopolysaccharide ‐induced sepsis in C57BL/6 mice
AbstractSepsis is a potentially fatal syndrome related to severe systemic inflammation developed by infection. Despite different antimicrobial therapies, morbidity and mortality rates remain high. Herbs along with cell therapy have been introduced as a promising option to improve the symptoms of sepsis. The present study aimed to evaluate the therapeutic effect of simultaneous administration of thyme essential oil (TEO) and endothelial progenitor stem cells (EPCs) on lipopolysaccharide (LPS)-induced sepsis in C57BL/6 mice. Sepsis was induced in C57Bl/6J mice by intraperitoneal injection of LPS, followed 2  h later by an i...
Source: Biotechnology and Applied Biochemistry - March 22, 2024 Category: Biochemistry Authors: Mohammad Mehdi Soltan Dallal, Maryam Siavashi, Samira Karimaei, Vahid Siavashi, Milad Abdi, Mehdi Yaseri, Seyed Alireza Razavi, Ronak Bakhtiari Tags: ORIGINAL ARTICLE Source Type: research

Combination MEG3 lncRNA and Ciprofloxacin dramatically decreases cell migration and viability as well as induces apoptosis in GC cells in vitro
In conclusion, combining MEG3 lncRNA with Ciprofloxacin may be an effective therapeutic approach for GC treatment by influencing the miR-14-BCl-2 axis, resulting in reduced cell viability, migration, and increased apoptosis. (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - March 19, 2024 Category: Biochemistry Authors: Dena Najafi, Goli Siri, Maryam Sadri, Omid Yazdani, Romina Esbati, Parvin Karimi, Ali Keshavarz, Amirreza Mehmandar ‐Oskuie, Mehmet Ilktac Tags: ORIGINAL ARTICLE Source Type: research