Current Status and Future Prospects of Cladosporium sp., a Biocontrol Agent for Sustainable Plant Protection
Biocontrol Sci. 2022;27(4):185-191. doi: 10.4265/bio.27.185.ABSTRACTThe fungal biocontrol agents, Cladosporium sp. have great economic importance on account of their beneficial effects on the integrated pest management (IPM) program. The different species of this genus can control different arthropod pests like sweetpotato whitefly, sugarcane woolly aphid, two-spotted mite, cotton aphid, cotton leaf worm, red spider mite, armyworm; and different plant diseases like- rice blast, wheat stripe rust, chrysanthemum white rust, etc. Chemical pesticides are a common practice by the user to protect their crops from these pests. Bu...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Md Touhidul Islam Source Type: research

The Effectiveness of Neutral Electrolyzed Water for Decontaminating the Spray Nozzles of Electric Tankless and Tank-Type Warm-Water Bidet Toilet Seats
Biocontrol Sci. 2022;27(4):193-200. doi: 10.4265/bio.27.193.ABSTRACTElectrolyzed water is a novel disinfectant that is widely used in the food industry. We conducted an experimental model-based study to determine the effectiveness of neutral electrolyzed water (NEW) for the daily nozzle cleaning of artificially contaminated tankless and tank-type bidet toilet seats. The toilet seats were designed to automatically self-clean the spray nozzles using tap water or NEW after each use or at specified intervals. The numbers of Pseudomonas aeruginosa and Escherichia coli microorganisms in the spray water were measured twice or thr...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Toru Iyo Keisuke Iwahori Source Type: research

Isolation, Screening and Identification of Native and New Bacillus subtilis with Strong Antifungal Compound against Fusarium oxysporum
Biocontrol Sci. 2022;27(4):201-208. doi: 10.4265/bio.27.201.ABSTRACTThe genus Fusarium causes a wide range of infections in human, animals and herbs. The purpose of this research was to investigate and identify the native strains of Bacillus subtilis playing an inhibitory role against Fusarium oxysporum by producing surfactin. B. subtilis was isolated from the soil of various parks in Tehran-Iran, and identified by biochemical tests. Growth inhibition zone, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of B. subtilis were determined. After purification of surfactin, quantitative and qual...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Fatemeh Rafiee Mohammad Reza Fazeli Abbas Akhavan Sepahi Zahra Noormohammadi Source Type: research

Simple and Rapid Detection of ESBL bla < sub > SHV < /sub > gene from an Urban River in Tokyo by Loop-Mediated Isothermal Amplification
Biocontrol Sci. 2022;27(4):209-215. doi: 10.4265/bio.27.209.ABSTRACTExtended-spectrum β-lactamases (ESBLs) are produced mainly by gram-negative bacteria in Enterobacteriaceae. One of the major types of ESBLs is sulfhydryl variable (SHV) -type ESBL. Herein, we attempted to develop a simple and rapid method for the detection of the ESBL blaSHV gene by loop-mediated isothermal amplification (LAMP) . The five-primer set designed could amplify blaSHV gene at an isothermal temperature of 65℃. The detection limit of the LAMP method with the LF loop primer was 1 copy/tube, which was 10,000-fold more sensitive than that of the c...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Masahiko Okai Rintaro Endo Masachika Takashio Masami Ishida Naoto Urano Source Type: research

Remote Bactericidal Effect of Anatase TiO < sub > 2 < /sub > Photocatalytic Nanoparticles Annealed with Low-Temperature O < sub > 2 < /sub > Plasma
Biocontrol Sci. 2022;27(4):217-222. doi: 10.4265/bio.27.217.ABSTRACTThe remote bactericidal effect of TiO2 photocatalyst, i.e., the bactericidal effect away from the photocatalyst, was successfully achieved using a humidified airflow. The TiO2 photocatalyst used was anatase-type TiO2 nanoparticles (NPs) annealed with a low-temperature O2 plasma. For comparison, anatase-type TiO2 NPs annealed in the air were used. The bacteria, Bacillus subtilis, were placed away from the TiO2 NPs. The plasma-assisted-annealed TiO2 NPs significantly inactivated 99% of the bacterial cells in 5 h, whereas the pristine and air-annealed TiO2 NP...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Retsuo Kawakami Yuki Takao Akihiro Shirai Takashi Mukai Source Type: research

Inactivation of SARS-CoV-2 by Commercially Available Disinfectants and Cleaners
In this study, the virucidal efficacy of forty-eight commercially available products were evaluated according to the standardized suspension method EN 14476 and the following results were obtained: Alcohol-based disinfectants, hand soaps, wet wipes, alkaline cleaners, quaternary ammonium compound sanitizers and oxygen bleach had great virucidal efficacy against SARS-CoV-2. Enveloped viruses such as SARS-CoV-2 are among the most susceptible of pathogens to formulated microbicidal actives and detergents, but as the results of this study showed, it is also necessary to pay attention to the concentration at the time of use and...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Y U Harada Hitomi Amo Takuto Ono Hiroki Murai Tadahiro Sasaki Tatsuo Shioda Masahito Ikawa Yoshihiko Hirata Source Type: research

Use of ATP Bioluminescence Assay to Evaluate Oral Streptococci
Biocontrol Sci. 2022;27(4):229-233. doi: 10.4265/bio.27.229.ABSTRACTThe statistical correlation between the number of oral streptococci and the results of ATP bioluminescence assay was examined and compared with the results from Streptococcus plate counts and an oral bacteria quantification system. Because a significant correlation was found between ATP (RLU) and the number of bacteria in the oral bacteria quantification system for all seven types of oral streptococci examined, ATP would reflect a conditions of oral hygiene. However, using this assay, it was observed it may be difficult to correctly evaluate bacteria that ...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Akari Watanabe Naofumi Tamaki Kenji Yokota Susumu Kokeguchi Hiro-O Ito Miwa Matsuyama Source Type: research

Current Status and Future Prospects of Cladosporium sp., a Biocontrol Agent for Sustainable Plant Protection
Biocontrol Sci. 2022;27(4):185-191. doi: 10.4265/bio.27.185.ABSTRACTThe fungal biocontrol agents, Cladosporium sp. have great economic importance on account of their beneficial effects on the integrated pest management (IPM) program. The different species of this genus can control different arthropod pests like sweetpotato whitefly, sugarcane woolly aphid, two-spotted mite, cotton aphid, cotton leaf worm, red spider mite, armyworm; and different plant diseases like- rice blast, wheat stripe rust, chrysanthemum white rust, etc. Chemical pesticides are a common practice by the user to protect their crops from these pests. Bu...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Md Touhidul Islam Source Type: research

The Effectiveness of Neutral Electrolyzed Water for Decontaminating the Spray Nozzles of Electric Tankless and Tank-Type Warm-Water Bidet Toilet Seats
Biocontrol Sci. 2022;27(4):193-200. doi: 10.4265/bio.27.193.ABSTRACTElectrolyzed water is a novel disinfectant that is widely used in the food industry. We conducted an experimental model-based study to determine the effectiveness of neutral electrolyzed water (NEW) for the daily nozzle cleaning of artificially contaminated tankless and tank-type bidet toilet seats. The toilet seats were designed to automatically self-clean the spray nozzles using tap water or NEW after each use or at specified intervals. The numbers of Pseudomonas aeruginosa and Escherichia coli microorganisms in the spray water were measured twice or thr...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Toru Iyo Keisuke Iwahori Source Type: research

Isolation, Screening and Identification of Native and New Bacillus subtilis with Strong Antifungal Compound against Fusarium oxysporum
Biocontrol Sci. 2022;27(4):201-208. doi: 10.4265/bio.27.201.ABSTRACTThe genus Fusarium causes a wide range of infections in human, animals and herbs. The purpose of this research was to investigate and identify the native strains of Bacillus subtilis playing an inhibitory role against Fusarium oxysporum by producing surfactin. B. subtilis was isolated from the soil of various parks in Tehran-Iran, and identified by biochemical tests. Growth inhibition zone, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of B. subtilis were determined. After purification of surfactin, quantitative and qual...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Fatemeh Rafiee Mohammad Reza Fazeli Abbas Akhavan Sepahi Zahra Noormohammadi Source Type: research

Simple and Rapid Detection of ESBL bla < sub > SHV < /sub > gene from an Urban River in Tokyo by Loop-Mediated Isothermal Amplification
Biocontrol Sci. 2022;27(4):209-215. doi: 10.4265/bio.27.209.ABSTRACTExtended-spectrum β-lactamases (ESBLs) are produced mainly by gram-negative bacteria in Enterobacteriaceae. One of the major types of ESBLs is sulfhydryl variable (SHV) -type ESBL. Herein, we attempted to develop a simple and rapid method for the detection of the ESBL blaSHV gene by loop-mediated isothermal amplification (LAMP) . The five-primer set designed could amplify blaSHV gene at an isothermal temperature of 65℃. The detection limit of the LAMP method with the LF loop primer was 1 copy/tube, which was 10,000-fold more sensitive than that of the c...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Masahiko Okai Rintaro Endo Masachika Takashio Masami Ishida Naoto Urano Source Type: research

Remote Bactericidal Effect of Anatase TiO < sub > 2 < /sub > Photocatalytic Nanoparticles Annealed with Low-Temperature O < sub > 2 < /sub > Plasma
Biocontrol Sci. 2022;27(4):217-222. doi: 10.4265/bio.27.217.ABSTRACTThe remote bactericidal effect of TiO2 photocatalyst, i.e., the bactericidal effect away from the photocatalyst, was successfully achieved using a humidified airflow. The TiO2 photocatalyst used was anatase-type TiO2 nanoparticles (NPs) annealed with a low-temperature O2 plasma. For comparison, anatase-type TiO2 NPs annealed in the air were used. The bacteria, Bacillus subtilis, were placed away from the TiO2 NPs. The plasma-assisted-annealed TiO2 NPs significantly inactivated 99% of the bacterial cells in 5 h, whereas the pristine and air-annealed TiO2 NP...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Retsuo Kawakami Yuki Takao Akihiro Shirai Takashi Mukai Source Type: research

Inactivation of SARS-CoV-2 by Commercially Available Disinfectants and Cleaners
In this study, the virucidal efficacy of forty-eight commercially available products were evaluated according to the standardized suspension method EN 14476 and the following results were obtained: Alcohol-based disinfectants, hand soaps, wet wipes, alkaline cleaners, quaternary ammonium compound sanitizers and oxygen bleach had great virucidal efficacy against SARS-CoV-2. Enveloped viruses such as SARS-CoV-2 are among the most susceptible of pathogens to formulated microbicidal actives and detergents, but as the results of this study showed, it is also necessary to pay attention to the concentration at the time of use and...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Y U Harada Hitomi Amo Takuto Ono Hiroki Murai Tadahiro Sasaki Tatsuo Shioda Masahito Ikawa Yoshihiko Hirata Source Type: research

Use of ATP Bioluminescence Assay to Evaluate Oral Streptococci
Biocontrol Sci. 2022;27(4):229-233. doi: 10.4265/bio.27.229.ABSTRACTThe statistical correlation between the number of oral streptococci and the results of ATP bioluminescence assay was examined and compared with the results from Streptococcus plate counts and an oral bacteria quantification system. Because a significant correlation was found between ATP (RLU) and the number of bacteria in the oral bacteria quantification system for all seven types of oral streptococci examined, ATP would reflect a conditions of oral hygiene. However, using this assay, it was observed it may be difficult to correctly evaluate bacteria that ...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Akari Watanabe Naofumi Tamaki Kenji Yokota Susumu Kokeguchi Hiro-O Ito Miwa Matsuyama Source Type: research

Current Status and Future Prospects of Cladosporium sp., a Biocontrol Agent for Sustainable Plant Protection
Biocontrol Sci. 2022;27(4):185-191. doi: 10.4265/bio.27.185.ABSTRACTThe fungal biocontrol agents, Cladosporium sp. have great economic importance on account of their beneficial effects on the integrated pest management (IPM) program. The different species of this genus can control different arthropod pests like sweetpotato whitefly, sugarcane woolly aphid, two-spotted mite, cotton aphid, cotton leaf worm, red spider mite, armyworm; and different plant diseases like- rice blast, wheat stripe rust, chrysanthemum white rust, etc. Chemical pesticides are a common practice by the user to protect their crops from these pests. Bu...
Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Md Touhidul Islam Source Type: research