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Influence of combined abiotic/biotic factors on decay of P. aeruginosa and E. coli in Rhine River water
In this study, the effect of salinity, light, and temperature and the presence of indigenous microbiota, on the decay/persistence of Escherichia coli and Pseudomonas aeruginosa in Rhine River water were tested during 7 days of incubation with varying salinity (0.4, 5.4, 9.4, and 15.4 ppt), with light under a light/dark regime (light/dark) and without light (dark), temperature (3, 12, and 20 °C), and presence/absence of indigenous microbiota. The results demonstrated that light, indigenous microbiota, and temperature significantly impacted the decay of E. coli. Moreover, a significant (p<0.01) four-factor interactive im...
Source: Applied Microbiology and Biotechnology - April 10, 2024 Category: Microbiology Authors: Sha Gao Nora B Sutton Thomas V Wagner Huub H M Rijnaarts Paul W J J van der Wielen Source Type: research

Manganese reductive dissolution coupled to Sb mobilization in contaminated shooting range soil
Appl Microbiol Biotechnol. 2024 Apr 10;108(1):295. doi: 10.1007/s00253-024-13133-2.ABSTRACTA "redox-stat" RMnR bioreactor was employed to simulate moderately reducing conditions (+ 420 mV) in Sb-contaminated shooting range soils for approximately 3 months, thermodynamically favoring Mn(IV) reduction. The impact of moderately reducing conditions on elemental mobilization (Mn, Sb, Fe) and speciation [Sb(III) versus Sb(V); Fe2+/Fe3+] was compared to a control bioreactor RCTRL without a fixed redox potential. In both bioreactors, reducing conditions were accompanied by an increase in effluent Sb(V) and Mn(II) concentrations, s...
Source: Applied Microbiology and Biotechnology - April 10, 2024 Category: Microbiology Authors: Lara Costa Mathieu Martinez Marcel Suleiman Rolf Keiser Moritz Lehmann Markus Lenz Source Type: research

Transcriptome analysis of Kluyveromyces marxianus under succinic acid stress and development of robust strains
Appl Microbiol Biotechnol. 2024 Apr 9;108(1):293. doi: 10.1007/s00253-024-13097-3.ABSTRACTKluyveromyces marxianus has become an attractive non-conventional yeast cell factory due to its advantageous properties such as high thermal tolerance and rapid growth. Succinic acid (SA) is an important platform molecule that has been applied in various industries such as food, material, cosmetics, and pharmaceuticals. SA bioproduction may be compromised by its toxicity. Besides, metabolite-responsive promoters are known to be important for dynamic control of gene transcription. Therefore, studies on global gene transcription under v...
Source: Applied Microbiology and Biotechnology - April 9, 2024 Category: Microbiology Authors: Du-Wen Zeng Yong-Qiang Yang Qi Wang Feng-Li Zhang Mao-Dong Zhang Sha Liao Zhi-Qiang Liu Ya-Chao Fan Chen-Guang Liu Lin Zhang Xin-Qing Zhao Source Type: research

Melanin depletion affects Aspergillus flavus conidial surface proteins, architecture, and virulence
Appl Microbiol Biotechnol. 2024 Apr 9;108(1):291. doi: 10.1007/s00253-024-13107-4.ABSTRACTMelanin is an Aspergillus flavus cell wall component that provides chemical and physical protection to the organism. However, the molecular and biological mechanisms modulating melanin-mediated host-pathogen interaction in A. flavus keratitis are not well understood. This work aimed to compare the morphology, surface proteome profile, and virulence of melanized conidia (MC) and non-melanized conidia (NMC) of A. flavus. Kojic acid treatment inhibited melanin synthesis in A. flavus, and the conidial surface protein profile was significa...
Source: Applied Microbiology and Biotechnology - April 9, 2024 Category: Microbiology Authors: Ondippili Rudhra Hariharan Gnanam Sivaramakrishnan Sivaperumal VenkateshPrajna Namperumalsamy Lalitha Prajna Dharmalingam Kuppamuthu Source Type: research

Pulchinenoside B4 ameliorates oral ulcers in rats by modulating gut microbiota and metabolites
This study demonstrated that the gut microbiota and metabolites were altered in the OU rat model, which were significantly restored to the normal level by B4, thereby showing good application prospects in the treatment of OU. KEY POINTS: • The first investigating the correlation between OU and gut microbiota. • A close correlation between metabolites and gut microbiota in OU disease was successfully identified. • Pulchinenoside B4 ameliorates oral ulcers in rats by modulating gut microbiota and metabolites.PMID:38592514 | PMC:PMC11003895 | DOI:10.1007/s00253-024-13099-1 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - April 9, 2024 Category: Microbiology Authors: Dewei Luo Li Yan Zhujun Wang Xiaofan Ji Na Pei Jing Jia Yingying Luo Hui Ouyang Shilin Yang Yulin Feng Source Type: research

Pulchinenoside B4 ameliorates oral ulcers in rats by modulating gut microbiota and metabolites
This study demonstrated that the gut microbiota and metabolites were altered in the OU rat model, which were significantly restored to the normal level by B4, thereby showing good application prospects in the treatment of OU. KEY POINTS: • The first investigating the correlation between OU and gut microbiota. • A close correlation between metabolites and gut microbiota in OU disease was successfully identified. • Pulchinenoside B4 ameliorates oral ulcers in rats by modulating gut microbiota and metabolites.PMID:38592514 | DOI:10.1007/s00253-024-13099-1 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - April 9, 2024 Category: Microbiology Authors: Dewei Luo Li Yan Zhujun Wang Xiaofan Ji Na Pei Jing Jia Yingying Luo Hui Ouyang Shilin Yang Yulin Feng Source Type: research

A candidate competitive ELISA based on monoclonal antibody 3A8 for diagnosis of contagious bovine pleuropneumonia
Appl Microbiol Biotechnol. 2024 Apr 8;108(1):290. doi: 10.1007/s00253-024-13127-0.ABSTRACTFor the development of a competitive ELISA (cELISA) to detect serum antibodies against the Mycoplasma mycoides subsp. Mycoides (Mmm) (strain PG1), the causative agent of contagious bovine pleuropneumonia (CBPP), all the proteins of this pathogen were analyzed. Then, a specific extracellular region of a transmembrane protein with the potential for diagnosis was identified. After that, a monoclonal antibody (Mab) named 3A8 was obtained using this extracellular region as an immunogen. Finally, a cELISA was established with the extracellu...
Source: Applied Microbiology and Biotechnology - April 8, 2024 Category: Microbiology Authors: Qi Wu Zhixin Ma Qiao Pan Tong Liu Yidan Zhang Jiuqing Xin Qingyuan Xu Source Type: research

Enhancing Escherichia coli abiotic stress resistance through ornithine lipid formation
In this study, we engineered different E. coli strains to produce unmodified OLs and hydroxylated OLs by expressing the synthetic operon olsFC. Our results showed that OL formation improved pH resistance and increased biomass under phosphate limitation. Transcriptome analysis revealed that OL-forming strains differentially expressed stress- and membrane-related genes. OL-producing strains also showed better growth in the presence of the ionophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP), suggesting reduced proton leakiness in OL-producing strains. Furthermore, our engineered strains showed improved heterologous viol...
Source: Applied Microbiology and Biotechnology - April 8, 2024 Category: Microbiology Authors: Leidy Patricia Bedoya-P érez Alejandro Aguilar-Vera Mishael S ánchez-Pérez Jos é Utrilla Christian Sohlenkamp Source Type: research

Epiberberine: a potential rumen microbial urease inhibitor to reduce ammonia release screened by targeting UreG
In conclusion, our study clearly indicates that epiberberine is a promising candidate as a safe and effective inhibitor of rumen microbial urease and provides an optimal strategy and suitable feed additive for regulating nitrogen excretion in ruminants in the future. KEY POINTS: • Epiberberine is the most effective inhibitor of rumen urease from Rhizoma Coptidis. • Urease accessory protein UreG is an effective target for design of urease inhibitor. • Epiberberine may be used as natural feed additive to reducing NH3 release in ruminants.PMID:38587649 | PMC:PMC11001712 | DOI:10.1007/s00253-024-13131-4 (Source: Applied ...
Source: Applied Microbiology and Biotechnology - April 8, 2024 Category: Microbiology Authors: Xiaoyin Zhang Zhanbo Xiong Yue He Nan Zheng Shengguo Zhao Jiaqi Wang Source Type: research

Epiberberine: a potential rumen microbial urease inhibitor to reduce ammonia release screened by targeting UreG
In conclusion, our study clearly indicates that epiberberine is a promising candidate as a safe and effective inhibitor of rumen microbial urease and provides an optimal strategy and suitable feed additive for regulating nitrogen excretion in ruminants in the future. KEY POINTS: • Epiberberine is the most effective inhibitor of rumen urease from Rhizoma Coptidis. • Urease accessory protein UreG is an effective target for design of urease inhibitor. • Epiberberine may be used as natural feed additive to reducing NH3 release in ruminants.PMID:38587649 | DOI:10.1007/s00253-024-13131-4 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - April 8, 2024 Category: Microbiology Authors: Xiaoyin Zhang Zhanbo Xiong Yue He Nan Zheng Shengguo Zhao Jiaqi Wang Source Type: research

Associations between the rumen microbiota and carcass merit and meat quality in beef cattle
In this study, we examined the associations between archaeal and bacterial taxa in the rumen microbiota of beef cattle and 12 different attributes, including hot carcass weight (HCW), dressing percentage, ribeye area (REA), intramuscular fat content, marbling score, fat thickness, yield grade, moisture content, purge loss, and shear force. There were significant correlations between the relative abundance of certain archaeal and bacterial genera and these attributes. Notably, Selenomonas spp. were positively correlated with live weight and HCW, while also being negatively correlated with purge loss. Members of the Christen...
Source: Applied Microbiology and Biotechnology - April 6, 2024 Category: Microbiology Authors: Devin B Holman Katherine E Gzyl Haley Scott Cara Service Nuria Prieto Óscar López-Campos Source Type: research