Exploring the potential of SrTi < sub > 0.7 < /sub > Fe < sub > 0.3 < /sub > O < sub > 3 < /sub > perovskite/chitosan nanosheets for the development of a label-free electrochemical sensing assay for determination of naproxen in human plasma samples
Anal Biochem. 2024 Mar 24:115513. doi: 10.1016/j.ab.2024.115513. Online ahead of print.ABSTRACTNaproxen is a nonsteroidal anti-inflammatory drug used to treat nonrheumatic inflammation, migraine, and gout. Therefore, the determination of naproxen in pharmaceutical and biological samples is of particular importance. In the present work, SrTi0.7Fe0.3O3 perovskite/Chitosan nanosheets were used to modify the surface of a glassy carbon electrode (GCE) for highly sensitive determination of naproxen. To ensure the successful synthesis of the perovskite nanosheets, morphological studies including scanning electron microscopy (SEM)...
Source: Analytical Biochemistry - March 26, 2024 Category: Biochemistry Authors: Roghayeh Mahmoudi-Maleki Mir Reza Majidi Hessamaddin Sohrabi Elham Mahmoudi Homa Fooladvand Ali Coruh Aligholi Niaei Source Type: research

Self-signal electrochemical identification of circulating tumor DNA employing poly-xanthurenic acid assembled on black phosphorus nanosheets
Anal Biochem. 2024 Mar 25;690:115512. doi: 10.1016/j.ab.2024.115512. Online ahead of print.ABSTRACTA self-signal electrochemical identification interface was prepared for the determination of circulating tumor DNA (ctDNA) in peripheral blood based on poly-xanthurenic acid (PXTA) assembled on black phosphorus nanosheets (BPNSs) acquired through simple ultrasonication method. The BPNSs with large surface area could be integrated with the xanthurenic acid (XTA) monomers by right of physisorption, and hence improved the electropolymerization efficiency and was beneficial to the enlargement of the signal response of PXTA. The a...
Source: Analytical Biochemistry - March 25, 2024 Category: Biochemistry Authors: Xinyu Yan Wei Zhang Jimin Yang Source Type: research

Polysaccharide-coated polystyrene microtiter plates for improved protein immobilization
Anal Biochem. 2024 Mar 22:115514. doi: 10.1016/j.ab.2024.115514. Online ahead of print.NO ABSTRACTPMID:38522810 | DOI:10.1016/j.ab.2024.115514 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - March 24, 2024 Category: Biochemistry Authors: Anton Plekhanov Sergey Shishlyannikov Source Type: research

Simultaneous determination of plasma protein binding of five C-glycosylflavones from TFDS by rapid equilibrium dialysis
In this study, the plasma protein binding of these compounds was determined for the first time in rat and human plasma by rapid equilibrium dialysis combined with HPLC-MS/MS method. The developed method was validated in terms of specificity, linearity, accuracy, precision, extraction effect, matrix effect, and stability. Schaftoside, vicenin-1, vicenin-2, and vicenin-3 exhibited moderate plasma protein binding, ranging from 56.6% to 61.5% in rat plasma and 55.0%-62.9% in human plasma. In comparison, isovitexin demonstrated a higher plasma protein binding in the range of 92.3-93.1% and 95.1-96.2% in rat and human plasma, re...
Source: Analytical Biochemistry - March 24, 2024 Category: Biochemistry Authors: Nan Ding Chen Chao Yishu Liu Peiyong Zheng Xue Li Ming Yang Source Type: research

Termostable and effective immobilized invertase for sucrose determination in fruit juices
In this study, immobilization of invertase enzyme was performed on a previously synthesized and characterized poly(N-vinylpyrrolidone-co-butylacrylate-co-N hydroxymethylacrylamide) terpolymer membranes by covalent bonding method. Glutaraldehyde(GA) was used as the crosslinker and Bovine Serum Albumin(BSA) was used as the binding agent. Optimum pH, temperature, amount of polymer, substrate concentration, amount of BSA and amount of GA values were determined for both free and immobilized enzyme. Optimum pH and temperature values were found as pH = 5.0, T = 55 °C, pH = 7.0 and T = 80 °C for free and immobilized enzyme, resp...
Source: Analytical Biochemistry - March 24, 2024 Category: Biochemistry Authors: Orhun Hakkoymaz Hidayet Maz ı Source Type: research

Polysaccharide-coated polystyrene microtiter plates for improved protein immobilization
Anal Biochem. 2024 Mar 22:115514. doi: 10.1016/j.ab.2024.115514. Online ahead of print.NO ABSTRACTPMID:38522810 | DOI:10.1016/j.ab.2024.115514 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - March 24, 2024 Category: Biochemistry Authors: Anton Plekhanov Sergey Shishlyannikov Source Type: research

Simultaneous determination of plasma protein binding of five C-glycosylflavones from TFDS by rapid equilibrium dialysis
In this study, the plasma protein binding of these compounds was determined for the first time in rat and human plasma by rapid equilibrium dialysis combined with HPLC-MS/MS method. The developed method was validated in terms of specificity, linearity, accuracy, precision, extraction effect, matrix effect, and stability. Schaftoside, vicenin-1, vicenin-2, and vicenin-3 exhibited moderate plasma protein binding, ranging from 56.6% to 61.5% in rat plasma and 55.0%-62.9% in human plasma. In comparison, isovitexin demonstrated a higher plasma protein binding in the range of 92.3-93.1% and 95.1-96.2% in rat and human plasma, re...
Source: Analytical Biochemistry - March 24, 2024 Category: Biochemistry Authors: Nan Ding Chen Chao Yishu Liu Peiyong Zheng Xue Li Ming Yang Source Type: research

Termostable and effective immobilized invertase for sucrose determination in fruit juices
In this study, immobilization of invertase enzyme was performed on a previously synthesized and characterized poly(N-vinylpyrrolidone-co-butylacrylate-co-N hydroxymethylacrylamide) terpolymer membranes by covalent bonding method. Glutaraldehyde(GA) was used as the crosslinker and Bovine Serum Albumin(BSA) was used as the binding agent. Optimum pH, temperature, amount of polymer, substrate concentration, amount of BSA and amount of GA values were determined for both free and immobilized enzyme. Optimum pH and temperature values were found as pH = 5.0, T = 55 °C, pH = 7.0 and T = 80 °C for free and immobilized enzyme, resp...
Source: Analytical Biochemistry - March 24, 2024 Category: Biochemistry Authors: Orhun Hakkoymaz Hidayet Maz ı Source Type: research

Comparison of two acquisition modes in mass spectrometry for quantification of 12 antibiotics, including beta-lactams, linezolid, fluoroquinolones and clindamycin: Multiple reactions monitoring (MRM) and fullMS/dd-MS < sup > 2 < /sup >
Anal Biochem. 2024 Mar 19;690:115507. doi: 10.1016/j.ab.2024.115507. Online ahead of print.ABSTRACTThe quantification of antibiotics, using mass spectrometry, for monitoring therapeutic drugs is a key benefit in infection management. After an easy work-up of plasma samples, analysis were performed using both two widely used acquisition modes: MRM for the triple quadrupole spectrometer and fullMS/ddMS2 for the HRMS to quantify twelve antibiotics. Comparison between the two acquisition modes were performed. Validation parameters and sample values were used as comparison criteria. The results indicated a good correlation betw...
Source: Analytical Biochemistry - March 21, 2024 Category: Biochemistry Authors: Catherine Feliu Celine Konecki Damien Vautier Jessica Rogier Claire Gozalo Yoann Cazaubon Zoubir Djerada Source Type: research

PhosAF: An integrated deep learning architecture for predicting protein phosphorylation sites with AlphaFold2 predicted structures
Anal Biochem. 2024 Mar 19;690:115510. doi: 10.1016/j.ab.2024.115510. Online ahead of print.ABSTRACTPhosphorylation is indispensable in comprehending biological processes, while biological experimental methods for identifying phosphorylation sites are tedious and arduous. With the rapid growth of biotechnology, deep learning methods have made significant progress in site prediction tasks. Nevertheless, most existing predictors only consider protein sequence information, that limits the capture of protein spatial information. Building upon the latest advancement in protein structure prediction by AlphaFold2, a novel integrat...
Source: Analytical Biochemistry - March 21, 2024 Category: Biochemistry Authors: Ziyuan Yu Jialin Yu Hongmei Wang Shuai Zhang Long Zhao Shaoping Shi Source Type: research

Comparison of two acquisition modes in mass spectrometry for quantification of 12 antibiotics, including beta-lactams, linezolid, fluoroquinolones and clindamycin: Multiple reactions monitoring (MRM) and fullMS/dd-MS < sup > 2 < /sup >
Anal Biochem. 2024 Mar 19;690:115507. doi: 10.1016/j.ab.2024.115507. Online ahead of print.ABSTRACTThe quantification of antibiotics, using mass spectrometry, for monitoring therapeutic drugs is a key benefit in infection management. After an easy work-up of plasma samples, analysis were performed using both two widely used acquisition modes: MRM for the triple quadrupole spectrometer and fullMS/ddMS2 for the HRMS to quantify twelve antibiotics. Comparison between the two acquisition modes were performed. Validation parameters and sample values were used as comparison criteria. The results indicated a good correlation betw...
Source: Analytical Biochemistry - March 21, 2024 Category: Biochemistry Authors: Catherine Feliu Celine Konecki Damien Vautier Jessica Rogier Claire Gozalo Yoann Cazaubon Zoubir Djerada Source Type: research

PhosAF: An integrated deep learning architecture for predicting protein phosphorylation sites with AlphaFold2 predicted structures
Anal Biochem. 2024 Mar 19:115510. doi: 10.1016/j.ab.2024.115510. Online ahead of print.ABSTRACTPhosphorylation is indispensable in comprehending biological processes, while biological experimental methods for identifying phosphorylation sites are tedious and arduous. With the rapid growth of biotechnology, deep learning methods have made significant progress in site prediction tasks. Nevertheless, most existing predictors only consider protein sequence information, that limits the capture of protein spatial information. Building upon the latest advancement in protein structure prediction by AlphaFold2, a novel integrated d...
Source: Analytical Biochemistry - March 21, 2024 Category: Biochemistry Authors: Ziyuan Yu Jialin Yu Hongmei Wang Shuai Zhang Long Zhao Shaoping Shi Source Type: research

Comparison of two acquisition modes in mass spectrometry for quantification of 12 antibiotics, including beta-lactams, linezolid, fluoroquinolones and clindamycin: Multiple reactions monitoring (MRM) and fullMS/dd-MS < sup > 2 < /sup >
Anal Biochem. 2024 Mar 19:115507. doi: 10.1016/j.ab.2024.115507. Online ahead of print.ABSTRACTThe quantification of antibiotics, using mass spectrometry, for monitoring therapeutic drugs is a key benefit in infection management for many hospitals. After an easy work-up of plasma samples, analysis were performed using both We aimed to evaluate the differences and agreements between the two widely used acquisition modes: MRM for the triple quadrupole spectrometer and fullMS/ddMS2 for the HRMS to quantify twelve antibiotics. Comparison between the two acquisition modes were performed. Validation parameters and sample values ...
Source: Analytical Biochemistry - March 21, 2024 Category: Biochemistry Authors: Catherine Feliu Celine Konecki Damien Vautier Jessica Rogier Claire Gozalo Yoann Cazaubon Zoubir Djerada Source Type: research

PhosAF: An integrated deep learning architecture for predicting protein phosphorylation sites with AlphaFold2 predicted structures
Anal Biochem. 2024 Mar 19:115510. doi: 10.1016/j.ab.2024.115510. Online ahead of print.ABSTRACTPhosphorylation is indispensable in comprehending biological processes, while biological experimental methods for identifying phosphorylation sites are tedious and arduous. With the rapid growth of biotechnology, deep learning methods have made significant progress in site prediction tasks. Nevertheless, most existing predictors only consider protein sequence information, that limits the capture of protein spatial information. Building upon the latest advancement in protein structure prediction by AlphaFold2, a novel integrated d...
Source: Analytical Biochemistry - March 21, 2024 Category: Biochemistry Authors: Ziyuan Yu Jialin Yu Hongmei Wang Shuai Zhang Long Zhao Shaoping Shi Source Type: research

Periodic magnetic modulation enhanced electrochemical analysis for highly sensitive determination of genomic DNA methylation
Anal Biochem. 2024 Mar 18:115509. doi: 10.1016/j.ab.2024.115509. Online ahead of print.ABSTRACTDNA methylation aberrations have a strong correlation with cancer in early detection, diagnosis, and prognosis, which make them possible candidate biomarkers. Electrochemical biosensors offer rapid protocols for detecting DNA methylation status with minimal pretreatment of samples. However, the inevitable presence of background current in the time domain, including electrochemical noise and variations, limits the detection performance of these biosensors, especially for low concentration analytes. Here, we propose an ultrasensiti...
Source: Analytical Biochemistry - March 20, 2024 Category: Biochemistry Authors: Yitao Liang Yu Jiang Lu Fang Zhen Dai Shanshan Zhang Yue Zhou Yu Cai Dong Wang Zhaoyang Wang Xuesong Ye Bo Liang Source Type: research